BSc Biochemistry Sem VI 2016 17 2016-17 BIOCHEMISTRY Paper 1 Metabolism Analytical Techniques Question Paper - Mumbai University | munotes

(2% Hours) [ Total Marks : 75
x (1) All the question are compulsory
(2) Internal choices have been given
to the right indicate marks
the MOST APPROPRIATE answer (any three): 03
acid will undergo . cycle of Beta
ii) Enzymes which digest fats
a) Lipases b) Hexokinase c)
iii) Acetyl CoA is converted to malonyl CoA with of a coenzyme
iv) The starting substance for the process of body formation is
vy) mediates during fatty acid are bound to
c) Biotin
2 Ae APPR answer
a) b)Hexokinase
utilization of ketone bodies by our body using biochemical
oxidation in for
inter mediates during fatty acid synthesis are
b) ACP Biotin
Describe the bjosynthesis of fatty acid using fatty acid synthetase enzyme
A~ - ii) the, bodies in the liver using biochemical
and significance in pregnancy and starvation
(any one):. ne
Dopamine is synthetised from amino acids
iv) is the steroid hormone in humans
V) Oxytocin be classified a
b) asteroid
in glycolysis and decreases inglycoleness
b) Increase in glyconeogenesis and in glycogenolysis
ine) Increase in glycogenesis and d es in glyconeogenesis
is synthetised fro amino acids
a) An acid residues
to show the formation of urea by urea cycle, showing all
es, and equations
the glycogen breakdown
c) in and elyconeogenesis
The fixst fraction obtained in differential 0:
i) . of
Dray showthe formation-of urea by urea cycle,
ii) not used as gradient
a) Glycerol in density gradient cetrifugation
iii) The visible light is generated in spectr c) maltose
ophometer by lamp
method b) Bi be estimated by
y) Analytical ultracentrifuge have methed c) Fiske
b) a max speed of 30,000.rpm S
been used to separate ino Q
vi) Preparative centrifugution is
a) The isolation of biologi
biological material for subsequentinvestigation
Determine the Concentration of DNA j 2 preparation
c) The study of pure molecules. ay T7134
ii) RCE
(c) Attempt jt is in 04
you determine the. coefficient of coloured solution
ii) of
"centrifuges. Write a note on analytical ultra contrifuge
uses principle of
acking gel in PAGE is protein molecules
Sodium dodeayl sulphate is used in SDS PAGE detergent
the types centrifuges
v) Proteins are separated by PAGE may be visualized by A
a) Bromophenol blue
b) Brilliant Blue S
B)Define attempt any one
i) Disuss the various supporting media of electrop, sis
to power pack
i) Discuss the factors which affect the of migration of sample in
and applications of
Explain the knoop's eriment . 2
i) Discuss of of Histidine with equation &
af and is. connect
i) any one applications of Beer law in the estimation of
Whe is Explain
and amt | of
i) Explain of buffer and ‘Ammonium persulphate in PAGE 3
Various dyes used for protein & nucleic acid separation by
Discuss the pf
B) Write True or False (any 3)
product of Lipogenenis is glycerol
ii) Action of insulin is supplemented with that of glucocorticot S. S
jii) Deamination of cysteine gives Acetyl CoA
iv) Ketonuria shows high level of ketone bodies in the blood
vi) Buffers are not required in electrophoretic experiment
ite True or False (any 3)
Action\of insulin is with that of glucocorticoids
ii) of cysteine Acetyl CoA
i) are in Experiment