BSc Biotechnology Sem IV 2015 16 2015-16 BIOTECH III Question Paper - Mumbai University | munotes

Questions asked in this paper

• 2) Figures to the right indi

1. Q3 Draw diagrams marks
2. Q1 A) Explain the followin
   • i) Discuss the two)
   • ii) In detail explain the Rho
   • iii) ‘Many eukaryotic mRNAs. b ependent termination of transcription in these sequences are rem not prokaryotic mRNAs contain introns’. Describe how
   • iv) In detail explain the t during the production of mRNA ranscription initiation process in eukaryotes Explain the followi ng (Any four) 8
   • i) Translation an
   • iv) Degeneracy of code An viii) formyl methionine
   • B) swer the following. (Any the process of initiation of translation in E.coli of elongation of translation in prokaryotes rief about the production of aminoacyl - RNA with diagram
   • iv) Write down the characteristics of the genetic code
   • A) Fill in the blanks (Any 4) 4
   • i) P AGE is a technique of separation of pased on their molecular weight and charge ( ii) The technique which separates charged particles using electric field is :
   • a) no charge b) charge anegative charge d) the above
   • a) anionic b) cationic c) positive none of the above
   • v) gel electrophoresis, the DNA is moved towards the
   • c) DNA does'nt move d) none of the above
   • vi) The cross-linking agent used for PAGE gel is AGE Stacking concetration is always than that
   • a) Greater b) lesser
   • B) Same d). May be greater or lesser. = Give Significance of the following (any two) (Any two) tical
3. Q1 Explain the migration of an ion in an electric field with suitable diagram Explain the effect of temperature on electrophoretic mobility Explain the native PAGE for protein separation Iv) an account on the applications of short notes on: (any a — Protein sorting in the cell:

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