BSc Biotechnology Sem IV ATKT 2016-17 ATKT BIOTECHNOLOGY 3 Question Paper - Mumbai University | munotes

Questions asked in this paper

1. Q1 Explain the following: (any four) 8 marks
2. Q4 Enhancers
3. Q5 core promoter
4. Q8 Sigma factor Answer the following: (any two). (12) a Elaborate on the E.coli RNA polymerases
5. Q2 In detail explain the Rho dependent termination of transcription in prokaryotes
6. Q3 Describe how the intervening sequence of prokaryotic pre- mRNA are removed
7. Q4 Explain the transcription initiation process in eukaryotes,
   • (A) Explain the following: (any four) 8
8. Q4 factors (Rf)
9. Q5 acyl (A) site
10. Q6 Wobble hypothesis
11. Q7 Degeneracy of code a
    • (B) Answer the foilowing: (any two) Explain the process of formation of initiation complex in translation of E.coli, 12
12. Q2 How does the step elongation in protein synthesis take place? Give the role of al! the factors involved in it In brief explain the amino acylation of tRNA with diagram
13. Q4 Write down the different characteristics of the genetic code j Vin the blanks (uy four) Protels are denatured by the SDS Proteins have the charge-to-mass ratio proteins More rapidly through the gel Do a Oot the above can be directly in gels by AY them with the dve OS. In isoelectric proteins are separated on the basis of their A, relative content of positively charged residue only content of Negatively charged residue only
    • D. content of positively and negatively charged residue native PAGE, proteins are separated on the basis of Proteins are separated in an SDS-PAGE expcriment on the basis of their
    • B. weight
14. Q6 What is ethidium bromide
    • A. buffer
    • B. dye 7, In agarose gel clectrophoresis, the DNA is moved towards the
    • A. cathode
    • C. uvesn't move
    • D. none of above
    • g. PAGE is a technique of separation of based on their molecular weight and charge density
    • A. Proteins
    • D. Amino acids
    • B) Give significance of the following (Any Two) 4
    • C) Answer the following (Any Two) 12
15. Q1 Explain the factors affecting electrophoretic mobility
16. Q2 Mass to charge ratio affects the electrophoretic mobility
17. Q3 Expalin the SDS PAGE for protein separation
18. Q4 Explain principle and uses of electrophoresis in biotechnology
19. Q4 Write Short Notes on. (Any Three) 15 marks
    • i) Rho-independent termination of transcription
    • iii) buffers used in electrophoresis
    • iv) Termination of Translation

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