BSc Biotechnology Sem IV ATKT 2018-19 ATKT Biotech Molecular Diagnostics Question Paper - Mumbai University | munotes

Questions asked in this paper

• 1) Attempt all questions

1. Q2 All questions carry equal marks
2. Q4 Use of log tables and non-programmable calculator is allowed
3. Q5 For Q.2,Q.3 and attempt A and B OR Cand D
4. Q1 Do as directed (Any fifteen) 15 marks
5. Q1 Give significance of molecular diagnostics
6. Q2 Give significance of using mixture of phenol and chloroform in DNA
7. Q3 State the use of proteinase K
8. Q4 State the significance of polyT or polyU oligomers in mRNA isolation
9. Q5 How depurination of larger fragment of DNA is is achieved during
10. Q6 Proteins are separated using
11. Q7 State true or false: Enzymatical amplification of RNA is called PCR
12. Q8 Give an application of Taq Pol
13. Q9 What is UNG?
14. Q10 Who is the Inventor of PCR?
15. Q11 Define Forward primer
16. Q12 State true or false: DNA can be found in aerosol
17. Q13 Give an application of thermostable DNA ligase 14 State true or false: Exonuclease-I can degrade primers
18. Q15 Give the full form of VNTR
19. Q16 State true or false: RFLPs can be used as original molecular targets for gene mapping, human identification, and parentage testing
20. Q17 In sickle cell anaemia valine is inserted into the polypeptide instead of
21. Q18 Gonorrhea is caused by the microbe
22. Q19 State true or false: Fragile X Syndrome leads to Mental Retardation
23. Q20 Define Clinical Genetics
    • Q.P.Code: 33973
24. Q2 A_ Explain restriction enzyme mapping. Elaborate on DNA probes and protein probes 07 Explain the initial steps in nucleic acid isolation depending on the nature 08 of the starting material 8 marks
25. Q2 D_ Explain in detail the DNA transfer methods from gel to membrane. 7 marks
26. Q3 A_ Explain the process of PCR. Discuss the process of synthesis of cDNA. 07 8 marks
27. Q3 C is Real time PCR and explain it in detail. 8 marks
28. Q3 D__ Discuss: Primer designing. Discuss molecular testing for Neisseria. 08 7 marks
29. Q4 B is an Informed Consent? Mention all the information that should be provided in an Informed Consent Form for Clinical Genetic testing explain the application of RFLP in detection of Sickle 08 7 marks
30. Q4 D_ Explain the classification of Genetic testing Indications. 7 marks
31. Q5 Write Short notes on any three of the following 15 marks
    • a. Specimen requirements for HIV viral assays
    • b. Patents relating to molecular diagnostics Physical methods used for control of PCR contamination
    • e. Any one Method used for probe labelling

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