B.Sc. (Biotechnology) Sem IV 2018 19 May 2018-19 BIOTECH MOLECULAR DAIGNOSTICS Question Paper - Mumbai University | munotes

Questions asked in this paper

• 1. Attempt all questions

1. Q2 All questions carry equal marks
2. Q4 Use of log tables and non-programmable calculator is allowed
3. Q5 and attempt A and B OR C and D
4. Q1 Do as directed: (Any fifteen) 15 marks
5. Q1 State true or false: Nucleic acids absorb light at 260 nm through the
6. Q2 State true or false: After release of DNA the cell, further purification requires removal of contaminating proteins, lipids, carbohydrates, and cell debris which is achieved by a mixture of Phenol
7. Q3 Fill in the blank: In Southern blotting, the double stranded DNA fragments in the gel must be denatured and transferred to a
8. Q4 Explain the role of restriction endonucleases
9. Q5 Fill in the blank: Proteins are separated using
10. Q6 Fill in the blank: Taq DNA polymerase enzyme from has facilitated the process of molecular diagnostics
11. Q7 In paternity testing, if a man lacked the allele that must have come from the baby’s father, then the DNA typing data would have proved that he is not the father; this is known as result
12. Q8 Give an example of chelator present in master mix
13. Q9 Fill in the blank: The type of PCR control which ensures that the enzyme is active, the buffer is optimal, the primers are priming the write sequences, and the thermal cycler is cycling appropriately is called as
14. Q10 Fill in the blank: is used to increase the effectiveness of UV light to decontaminate and maintain pre-PCR area
15. Q11 Fill in the blank: The Tth polymerase is isolated from
16. Q12 Give an example of: Monovalent cations present in PCR master mixture Define the term ‘amplicons’
17. Q14 What is the significance of thermocyclers?
18. Q15 State true or false: Presence of quasi species of HIV is due to the error prone nature of the reverse transcriptase enzyme
19. Q16 Fill in the blank: It is the time period between exposure to HIV and before seroconversion is known as
20. Q17 State true or false: Pace-2 is a second generation hybridisation assays used for N.gonorrhoeae
21. Q18 Fill in the gene analysis is done to detect fragile X
22. Q19 Fill in the blank: Intrapartum transmission of N.gonorrhoeae produces condition in neonates
23. Q20 Fill the blank: __ is the process of determining an individual's DNA characteristics, which are as unique as fingerprints What is molecular diagnostics? Explain in detail the of mutation detection techniques in molecular diagnostics
24. Q2 B_ Elaborate on FISH as a hybridisation technique. 7 marks
25. Q2 C_ Discuss the organic and inorganic method for isolation of DNA. 8 marks
26. Q2 D_ Explain western blotting in detail. 7 marks
27. Q3 A_ Give working principle of PCR. any three components of PCR. 07 What is the function of DNA polymerase in PCR? What are the different 08 types of DNA polymerases used in PCR method? 8 marks
28. Q3 D How to control contamination in PCR? Discuss uses of genetic testing. 08 Elaborate on HIV and clinical significance of viral load in the serum 07
29. Q4 C How RFLP is used to detect sickle cell anaemia? 8 marks
30. Q4 D_ Explain clinical significance of N.gonorrhoeae. 7 marks
31. Q5 Short notes on any three of the following 15 marks
    • a. Northern blotting
    • b. Any one method for labelling of a probe
    • e. Any one commercial assay available to detect HIV1 virus

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