BSc Biotechnology Sem IV ATKT BIOTECH MOLECULAR DIAGNOSTICS Question Paper - Mumbai University | munotes

Questions asked in this paper

• 1. Attempt all questions

1. Q2 All questions carry equal marks
2. Q4 Use of log tables and non-programmable calculator is allowed
3. Q5 For Q 4 attempt A and B OR C and D
4. Q1 Do as directed (Any fifteen) 15 marks
   • l. What is CTAB Define restriction endonuclease
5. Q3 What is the role of primary and secondary antibody in western
6. Q4 What is the role.of phenol in DNA extraction? Difference between probe and primer
7. Q6 Write the name of any one method utilized in protein purification Ts Define personalized medicine
8. Q8 What is random hexamer pimer
9. Q9 What is oligo dT primer? What is the full form of LCR? What are the three different temperatures used in PCR reaction
10. Q13 What are psoralens
11. Q14 What is hot starts PCR
12. Q15 Write down the full form of RFLP
13. Q16 What is genetic counselling
14. Q17 Any one symptom of sickle cell anaemia
15. Q18 What do you mean by case study? 19, Name any one method to diagnose neisseria infection
16. Q20 is molecular diagnostics? Elaborate the commercializing of molecular diagnostics. 08 personalized medicine and theronastics. 07 Mechanism of northern blotting and northern hybridization. 08 Write about the electrophoretic techniques to detect DNA and proteins. 07 Write about the components of a typical PCR reaction. 08 are the essential features to be checked while designing a primer. 07
17. Q3 C Write about the detection of PCR amplicons
18. Q3 D_ Elaborate the process of RT-PCR. 7 marks
19. Q4 A_ Using RFLP technique how you will detect sickle cell anaemia. Write about the molecular testing of neisseria 07 8 marks
20. Q4 C Write one case study of carrier testing. What are the ethical, legal and social issues of genetic testing. 07 Write Short notes on any three of the following 15 8 marks
    • a. A short note on FISH,
    • b. Principle of ligase chain reaction What is molecular diagnostics and its role in post genomic era
    • d. Clinical application of probes
    • e. A short note on personalized medicine

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