BSc Biochemistry Sem VI 2014 15 2014-15 Paper L Add Question Paper - Mumbai University | munotes

Questions asked in this paper

1. Q2 All questions are compulsory
2. Q1 A. Define the following (any Four) 3 marks
   • B. Explain the following: (any Two) 6
   • i. Write down the stalagmometric method for surface tension What do you mean by flocculation?
   • iii. Briefly mentioned about any two properties of colloids?
   • iv. Describe percentile method of solute concentration
   • C. Answer in brief : (any One) 6
   • i. Explain : Hoff,s law
   • ii. Briefly describe how different factors affecting diffusion of solute in solution QU) A. Answer the following (any Four) [8]
   • i. What is a suspension medium?
   • ii. Define isotonic solution
   • iii. Name any 2 properties of C, elegans which makes it a model organism
   • iv. Explain the process of sonication What is osmosis? ‘
   • vi. Give any 4 advantages of D. melanogaster as a model organism
   • vii. are tissue studies?
   • viii. Give examples of cell fractionation techniques
   • B. Explain the following: (any Two) 16]
   • i. What is cell rupture?
   • ii. What is significance of organ studies?
   • iii. Importance of a model
   • iv. What are factors of suspension medium?
   • C. Answer in brief : (any One) 16]
   • i. Write a note on significance. of Drosophila organisms in
   • ii. Write a brief note on tissue techniques and significance of cel] disruption Q A: Answer the following (any Four) 3
   • i. How does the distance between specimen affect the resolution of image formed 2 ii, What is the RI of air? ili. Define condenser,
   • iv. What is the function of a prism?’ What is the function of objective lens?
   • vi. Define focal length What is the function of Dissection microscope? Name the lens used by
   • B. Answer the following: (any Two) ‘
   • i. the working of Scanning electron microscope li. Draw the focussing of light with the help of lenses Define refractive index with an example
   • iv. Explain the phenomenon of fluorescence
   • C. Answer in brief: (any
   • i. Explain the working of confocal microscope ii. Explain the working of
3. Q1 V] 1.(A) Answer any one of the following: ! unit of viscosity? ii. How osmosis help in diagnosis of
4. Q1 (B) Answer of the following: (any
   • i. is 1 mole of solute dissolved in 1000g of solvent (molality/ normality/ molarity)
   • ii. 9ml saline dissolved in 100 mI water means.saline.(9%/ 9gm%/ 9mi%)
   • iv. Colloidial state is denoted by particle size range from
   • v. Rate of diffusion proportiotional to size of particle
   • vi. The energy of . denoted by y (surface tension/ viscosity/ osmotic pressure) 2.(A) Give example of one the following [2]
   • i. Technique of cell rupture. fi.Any one unicellular model organism
5. Q2 (B) Answer of the following: (any Three) 3 marks
   • i. Name a model organism which is easy to store Name a cell rupture technique which involves freezing and crushing Name any one eukaryotic model organism
   • iv. Name any one organism which has homologous genes with human genes
   • v. Give any use of homegeniser
   • vi. Give any one importance of biochemical investigations 3.(A) Give application in industry of one the following:
   • i. Microorganisms
6. Q3 (B) Answer of the following: (any Three) What is the function of the mechanical stage in a microscope? Give examples of simple stains used in the laboratory, What is the highest magnification of objective lens in a compound microscope? 3 marks
   • iv. Name the lens employed in electron microscope,
   • v. compound used in shadowing a specimen in TEM Vi. uch kind of microscope i i Is used in the (ory for Studying simple structures

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