BSc Biotechnology Sem II 2022 2023 Apr 2023 BIOTECHNOLOGY P 4 BIOCHEMISTRY Question Paper - Mumbai University | munotes

Questions asked in this paper

• 1. Attempt all questions

1. Q2 All questions carry equal marks
2. Q4 and Q 4 attempt A and B OR C and D directed (Any fifteen) 15 Which groups are involved in peptide bond formation?
3. Q2 Name chemical reagent which can be used for protein precipitation by process of ‘salting out’ Proteins in which the ratio (Lys + Arg) (Glu + Asp) is less than | are referred to as acidic proteins. (True OR False)
4. Q4 Enlist the covalent bonds found in tertiary structure of proteins Give an example of fibrous proteins
5. Q6 The alpha-helix is stabilized by extensive bonding Serine, threonine and tyrosine containing amino acids
6. Q8 Explain the term co-enzyme
7. Q9 State true affinity of the enzyme for substrate,
8. Q10 What do you mean by irreversible inhibition? Give one significance of the enzyme alkaline phosphatase What are isozymes? State true false: Extremes of pH does not denature the enzyme
9. Q14 What is a stationary phase in a paper chromatography?
10. Q15 Give full form of the abbreviation HPLC Give an equation for the Beer-Lambert’s law What is the partition coefficient? What is the significance of the molar extinction coefficient? 19, What is the use of the Retardation Factor in chromatography? Which instrument will need a cuvette? VCD FYBT SEMI BIOTECHNOLOGY-P 4- Biochemistry 75 MARKS 2HRS 30MINS Elaborate on tertiary structure of proteins. 08 Draw structure of amino acids which have a Sulfur group. 07 Discuss the properties of proteins. 08
11. Q2 D_ Give an account on isoelectric pH of proteins. 7 marks
12. Q3 A_ With appropriate examples explain how enzymes are classified on the basis of the reactions they catalyse 8 marks
13. Q3 B_ Elaborate on non-competitive enzymes. 7 marks
14. Q3 C_ Discuss the various factors that influence the reaction velocity of 8 marks
15. Q3 D_ Describe the applications of enzymes in various industries. 7 marks
16. Q4 What are the principles and components of chromatography? Give an account of column chromatography by giving suitable examples. 07 Compare and contrast paper and Thin Layer Chromatography. 08 8 marks
17. Q4 D_ Give an account of colorimetry. 7 marks
18. Q5 Short notes on any three of the following reaction with amino acids 15 marks
    • d. of coloured compounds
    • e. Factor in chromatography

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