BSc Biotechnology Sem II 2014 2015 2015 ATKT Paper I Question Paper - Mumbai University | munotes

Questions asked in this paper

1. Q0 (A) Name the following (any two) ‘ation in frozen state and then Process in which organisms are subjected to extreme dehydration in frozen state 2, The lowest temperature at which a microbial suspension is killed in 10 mins 3, Radiations which have enough energy to knock electrons away from molecules and i Halogen used as disinfectant for municipal water supplier and swimming pools
   • (B) Explain the following terms (Any three)
   • (C) Answer the following (Any two) Explain Filtration as a physical agent in controlling microorganisms, 12
2. Q2 Explain tube - dilution and agar plate techniques for the evaluation of antimicrobial
3. Q3 Explain the different methods With respect to moist heat that can be used for control of
4. Q4 Describe in brief the use of chemical agents in contro] of microorganisms with respect to and sterilizing gases, II (A) Explain the following terms (Any four) (08)
5. Q6 Germplasm conservation the following. (Any two) (12) Explain the process of development in Root-tip culture and Embry i mbryo culture techniques used in the of plant tissue culture Write a note on of media and culture room used for the inorganic used for plant tissue culture, q What is meant by totipotency of plant cell? And mention the importance in plane Give signitionnce of the following in animal cell culture (Any two ) State whether the following are true or false, (Any four) cell line is utilized for the production of antibodies > The eancerous cell lines ave maintained as a adherent culture
6. Q3 The optimum concentration for animal cell culture is 1.5%, 4 marks
7. Q4 Subculturing of'animal cell line is Known as pouring
8. Q5 DMEM stands for
9. Q6 The specific culture vessels used for animal cell culture are known as M flasks
10. Q7 The air direction of the vertical laminar is parallel to the surface of working bench Serum is used as cell disaggregating agent in animal cell culture,
    • (C) Explain the following, (Any two) Limitations of'animal cell culture 12
11. Q2 Working of Vertical laminar air flow hood with suitable diagram
12. Q3 Advantages of serum containing media
13. Q4 Initiation of animal cell culture. 3 marks
14. Q1 V Write a note on the following . (Any Three)
15. Q1 Somatic hybridisation
16. Q3 Ultra violet rays as a physical agent for the control of
17. Q4 Aldehydes as chemical in control of microorganisms Layout of animal cell culture laboratory Physiochemical factors of animal cell culture

Read from the scan above, so a character or two may differ. The scan is the original.