B.Sc. (Biotechnology) Sem II 2018 19 Apr 2018-19 BIOTECH BIOTECHNOLOGY I TISSUE CULTURE & SCIENTIFIC WRITING & COMMUNICATION Question Paper - Mumbai University | munotes

Questions asked in this paper

1. Q1 All questions are compulsory
2. Q2 Figures to right indicate marks
3. Q1 Do as directed (ANY 15) 15 marks
4. Q1 Name one method for the isolation of single cell from plant organ in plant tissue culture
5. Q2 is the cell characteristics in which the potential for forming all the cell types in an adult plant is retained
6. Q3 Give one example of plant tissue culture medium
7. Q4 Give the significance of antibiotic used in PTC medium
8. Q5 Name the German scientist who developed the concept of invitro cell cultures of the plant tissue
9. Q6 Define—Callus
10. Q7 What does MLMH stands for in plant tissue culture medium?
11. Q8 Define:Feeding of cells
12. Q9 Define: Passage in animal cell culture
13. Q10 State true or false: Tumor cells give rise to continuous cell lines
14. Q11 State true or false: Cells from normal tissues show density dependency and contact inhibition at
15. Q12 Give one example of intracellular activity in animal cell
16. Q13 Fill in the blank: Tissue culture was first developed by
17. Q14 Fill in the blank: tissue was first used as first source for cell culture
18. Q15 Power point presentation is used for writing the research report.(State True or False)
19. Q16 Explain the term plagiarism
20. Q17 Language is one of the physiological barrier of communication. (State true or false)
21. Q18 Enlist any two examples of verbal communication
22. Q19 is a type of communication that employs gestures and body language
23. Q20 Communication is way process
24. Q2 A) Explain in detail the organization and role of culture room and transfer area in plant tissue Discuss the importance of totipotency in plant science. (07)
25. Q2 C)What are macronutrients? Discuss the role of macronutrients and any two plant growth hormone in plant tissue culture medium. (08)
26. Q2 D) Discuss the protocol for callus culture of the carrot root in detail. 7 marks
27. Q3 A) Define primary culture? Explain how to obtain a primary cell culture. 8 marks
28. Q3 B) Give methods of sterilization of animal tissue culture media. 7 marks
29. Q3 C) Write a note on any eight applications of animal tissue culture. 8 marks
30. Q3 D) Draw a neat labeled diagram of carbon dioxide incubator and explain its use in animal cell
    • A) Explain the process of communication in brief. 8
    • B) Enlist the components of research paper and elaborate any two components. 7
    • C) Read the following abstract and answer the following questions. Abstract: A wide range of medicinal and aromatic plants (MAPs) have been explored for their essential oils in the past few decades. Essential oils are complex volatile compounds, synthesized naturally in different plant parts during the process of secondary metabolism. The goals of the study were to determine and compare the phytochemical screening, antioxidant and antimicrobial activities of the essential oils of Azadirachta indica and. Vitex negundo. The phytochemical screening of the twoplants showed presence of alkaloids, carbohydrates, glycosides, phenolic andtannin compounds, flavonoids, saponins, lipids and terpenoids. About the antioxidant potential, Azadirachta indica showed a higher ability to scavenge free radicals as compared to Vitex negundo, which was investigated by (DPPH) radical scavenging assay. Regarding the antimicrobial activity, Vitex negundo showed a better ability to inhibit the growth of pathogenic micro-organisms as compared to Azadirachta indica, which was investigated by the Agar diffusion method a suitable title to the research work 11.What is the goal of the study? among the two plant exhibited better antioxidant activity? 8
    • iv.Give four key words for the research work based on the abstract
    • D) Explain the important differentiating points of verbal and non-verbal communication
31. Q5 Write short notes on: (Any three) 15 marks
32. Q1 Use of rotary shakers in PTC lab
33. Q2 Importance of callus culture
34. Q3 Inverted microscope
35. Q4 Importance of effective communication
36. Q5 Growth factors

Read from the scan above, so a character or two may differ. The scan is the original.