BSc Biotechnology Sem II 2014 2015 2015 Paper I Question Paper - Mumbai University | munotes

Questions asked in this paper

1. Q2 Filters employed in laminar air flow biological safety cabinets to remove 99.97% of
2. Q3 Shortest time needed to kill all organisms in a microbial suspension at a specific temperature and under defined conditions
3. Q4 Halogen used as skin antiseptic, which kills by oxidizing cell constituents and iodinating cell
   • (B) Explain the following terms (Any three) 6
4. Q5 Tyndallisation 6. radiations
   • (C) Answer the following (Any two)
5. Q1 Briefly describe alcohols and aldehydes as chemical antimicrobial agents
6. Q2 Give the conditions influencing the effectiveness of antimicrobial agents
7. Q3 Describe Phenol Coefficient method as a method for evaluating chemical antimicrobial agents
8. Q4 Give brief account on radiations as physical agent in controlling microorganisms
   • (A) Explain the following terms (Any four) 8
9. Q11 (B) Answer of the following.(Any two) 12 marks
10. Q1 Write about the history of development of tissue culture technique with respect to plant tissue Write about the use of growth regulators in media for plant tissue culture. Tah
11. Q3 note on Aseptic culture techniques maintained during the plant tissue culture
12. Q4 What is callus tissue? Describe the protocol for callus culture Q (A) Give significance of the following in animal cell culture ( Any two ) (04)
13. Q2 Trypsin 4. BSS State whether the following statements are true or false. (Any four) (04)
14. Q1 Subculturing of animal cell line is known as passaging
15. Q2 The optimum CO) concentration required for animal cell culture is 0.5 %
16. Q3 Trypsin is used as cell disaggregating agent in animal cel! culture
17. Q4 If Trypsin is added to the animal cell culture medium , addition of various growth factors can
18. Q5 The specific culture vessels used for animal cell culture are known as T
19. Q6 DMEM stands for Dolphin’s essential medium
20. Q7 The cancerous cell lines are maintained as a suspension culture
21. Q8 The air flow direction of the vertical laminar air flow is perpendicular to the surface of
    • (C) Explain the following. (Any two) 1, Applications of cell culture 12
22. Q2 Physiochemical requirements of animal cell culture
23. Q3 Layout of the animal cell culture laboratory Working of incubator with suitable
24. Q1 V Write a note on the following. (Any Three) 15 marks
25. Q2 Genetic transformation Dry heat as a physical agent for control of microorganisms 4, Ethylene oxide as antimicrobial agent History of animal cell
26. Q6 Chemically defined

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