BSc Biochemistry Sem VI 2019 20 Nov 2019-20 ATKT BIO CHEM METABOLISM AND ANALYTICAL TECHNIQUES BIOCHEMISTRY PAPER I Question Paper - Mumbai University | munotes

Questions asked in this paper

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1. Q1 All the questions are compulsory. Choice is internal
2. Q2 Figures to the right indicate full marks
3. Q3 All questions carry equal marks
4. Q1 (A) the MOST APPROPRIATE answer (any three): 3 marks
5. Q1 A fatty acid with 20 carbon atoms will undergo cycles of oxidation
   • ii) Animals cannot convert fatty acid into glucose because
   • a) acetyl coA cannot be converted to pyruvate
   • b) of absence of malate synthase
   • c) of absence of malate dehydrogenase ili) number of NADH + H molecules are produced,, after 6 "turns" of the B-oxidation pathway
   • iv) is not a ketone body
   • v) ACP is abbreviation of v1) NADPH is the reducing agent needed in the process of
6. Q1 (B) Answer in brief any one of the following: 2 marks
7. Q1 State the site/s of lipolysis and ketogenesis li) State true or false giving reason- Fatty acids are the preferred fuel for “running” the heart (cardiac muscle) and the brain Attempt any one of the following: 4
8. Q1 Write a short note on the FAS complex li) For the metabolic processes - ketogenesis, lipogenesis or B-oxidation pathway; select the appropriate biomolecules, with which they are associated. The responses may be used more than once or not be used at all
   • Q.P. Code: 34901 Answer any one of the following: 6
9. Q1 Schematically represent synthesis of a saturated fatty on the utilization of ATP in li) Elaborate on ketone body formation and its significance in diabetes mellitus
10. Q2 (A) the MOST APPROPRIATE answer (any three): 3 marks
11. Q1 In A chain of Insulin molecule the C-terminal amino li) Thiocynate competes with uptake mechanism ill) Glycogen phosphorylase is active in state
    • iv) Oxidative deamination is the conversion of an amino
    • a) group from an amino acid to.a keto acid b) acid to a keto-acid and ammonia
    • c) acid to a carboxylic acid and ammonia
    • v) is a lipid soluble hormone
    • a) Insulin b) Tetraiodothyronine v1) Transaminases are present in
12. Q2 (B) Attempt in brief any one: 2 marks
13. Q1 State true or false, giving reasons: Amino acid degradation is similar to any other catabolic
    • ii) Define and explain the term- Myxedema
14. Q2 (C) Write a noteonany one of the following : 4 marks
15. Q1 Deamination reactions li) Physiological role of glucocorticoids
16. Q2 (D) Write detailed answers to any one of the following: 6 marks
17. Q1 Discuss the effect of epinephrine on glycogen synthesis and its breakdown li) Elaborate on Krebs -Henseleit cycle and give its significance
18. Q3 (A) Choose the MOST APPROPRIATE answer (any three): 3 marks
19. Q1 wavelength ranges is NOT associated with UV spectroscopy
    • Q.P. Code: 34901 li) A series of 3 coloured glass plates of equal thickness are placed in a light beam. Each sheet absorbs half of the light incident upon it. The intensity of the light transmitted by the third glass ili) The wavelength of light source is 560 nm. is the corresponding wave number
    • a) 0.178 x 102 cm-1
    • iv) High speed centrifuge operates at the maximum speed upto
    • v) Rayleigh scatterring is used in centrifuge Vi) compound/s can be used as denisty gradient substance
20. Q3 (B) and explain any one: 2 marks
21. Q3 (C) Describe the applications/uses of. any one of the following: 4 marks
    • i) Colorimeter
22. Q3 (D) Write detailed answers for any one of the following: 6 marks
23. Q1 Differentiate between rate zonal and isopycnic centrifugation. Add a note on sedimentation li) Write the (a) Derivation (b) limitations and (c) applications of Beer- Lambert law
24. Q4 (A) Choose the MOST APPROPRIATE answer (any three): 3 marks
    • i) TEMED is used as a li) In electrophoresis proteins will migrate to
    • a) cathode (b) anode
    • c) cathode or anode and is dependent on charge on the protein iil) In SDS-PAGE seperation is
    • (a) molecular weight (b) shape (c) shape and molecular weight Iv) The pH of resolving gel is that of stacking gel
    • (a) more than less than same as
    • v) The electrophoretic mobility denoted by y is denoted as
    • Q.P. Code: 34901 Vi) blotting technique uses probes to detect RNA, post electrophoresis
25. Q4 (B) Give the role of any one of the following:
26. Q4 (C) Write short notes on any one of the following: 4 marks
27. Q1 Use of electrophoresis in nucleic acids separation li) Support material used in electrophoresis
28. Q4 (D) any one the following: 6 marks
    • i) Write an elaborative note on NATIVE PAGE li) Justify: "Electrophoresis is a technique governed by various factors"
29. Q5 (A) Write short note on any one of the following: 3 marks
30. Q1 Acyl shuttle li) Utilization of ketone bodies
31. Q5 (B) a note on any one of the following: 3 marks
    • i) Insulin
    • ii) Transamination
32. Q5 (C) in brief any one of the following: 3 marks
33. Q1 State true or false giving reasons: No relation exists between RCF and RPM li) Write a note on construction and working of a simple colorimeter
34. Q5 (D) Answer in brief any one of the following: 3 marks
35. Q1 Describe the principle of electrophoresis and enlist two buffers used for the same li) Write a note on application of electrophoresis in protein study State true or false (any three) 3
    • i) Oxytocin is a female hormone li) Acetyl coenzyme A is associated with ketolysis Molar absorptivity is not constant for any single biomolecule Extinction and absorbance are antonyms Vi) Centripetal force and centrifugal forces act in opposite direction

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