B.Sc. (Biotechnology) Sem II 2022 2023 Apr 2023 FUNDAMENTALS OF BIOTECHNOLOGY II Question Paper - Mumbai University | munotes

Questions asked in this paper

• 1. Attempt all questions

1. Q2 All questions carry equal marks
2. Q4 For and Q4 attempt A and B OR C and D directed (Any fifteen) 15 is one of the oldest practiced biotechnology processes (Fuel ethanol production, Beer and wine production, Antibiotic production, Enzyme Give an example of fermented food product of indigenous origin What is putrefaction?
3. Q4 State true or false. Fungus requires more moisture than bacteria to grow
4. Q5 Vinegar is made up of :
5. Q6 Name any method of food preservation Give full form of the abbreviation HACCP
6. Q8 State True or False: Unipotent stem cells can only produce cells of one kind, which is their own type
7. Q9 Give any one example of killed vaccine
8. Q10 Give any one use of monoclonal antibodies
9. Q11 Give any one application of stem cells
10. Q12 Name any one type of cells which can be used in tissue cultures for the production of viral vaccines
11. Q13 Give any one example which can be used as a vector for recombinant vector
12. Q14 What does “E” stand for in EcoRI?
13. Q15 The enzyme removes the phosphate moiety at the 5’-end of a DNA
14. Q16 What are the different requirements needed for Type I endonucleases activity? VCD/ CLASS: FYBT SEMII SUB- PAPER 1 Fundamentals of
15. Q17 | What do you mean by vector?
16. Q18 The is a process by which bacterial DNA fragments are introduced via natural bacteriophage vectors that infect the bacterial strain
17. Q19 Give one example of bacteria used as a Host cell in cloning
18. Q20 Which restriction enzyme gives ended tails after digestion? What are the criteria for the assurance of fitness or unfitness of food for 08 explain the quick method or the trickle method of vinegar 07 Give an account of classification of food by ease of spoilage. 08
19. Q2 D How would you prepare sauerkraut in the laboratory and which parameters would you check for its quality brief account on live ,attenuated vaccines and killed vaccines 08 7 marks
20. Q3 B_ Describe monoclonal antibody preparation in brief and add a note on its applications brief account on virus vaccine production. 08 7 marks
21. Q3 D_ Describe the type of stem cells and their significance in brief. 7 marks
22. Q4 A_ What is the plasmid? Discuss the essential features of plasmid vectors. 8 marks
23. Q4 B_ Explain the construction of DNA with the help of a diagram. 7 marks
24. Q4 C_ Discuss different Natural Parasexual Models of Gene Transfer in prokaryotes. 8 marks
25. Q4 D_ Give a detailed note on microinjection and electroporation techniques. Write a short note on any three of the following. 15 d Blue and white screening 7 marks

Read from the scan above, so a character or two may differ. The scan is the original.