BSc Biochemistry Sem III 2019 2020 Oct 2020 BIO CHEM BIOCHEMISTRY PAPER I Question Paper - Mumbai University | munotes

Questions asked in this paper

1. Q1 All the questions are compulsory. Choice is internal
2. Q2 Figures to the right indicates full marks 3.All questions carry equal marks
3. Q1 a) State True or False [4M]
   • i. For an acidic solution at
   • ii. NH.OH is the conjugate base of iil. Lysine is the simplest amino acid
   • iv. A solution with pOH 8 is acidic
   • b) Attempt any three of the following [9M]
   • i.Give titration curve of glycine What is pH and pKw ili. Explain phosphate buffer system
   • iv.Define physiological buffer and Henderson Hasselbalch equation
   • v . Explain buffering capacity
   • vi .Draw titration curve of aspartic acid
4. Q1 Attempt any two of the following [12M]
   • i. Write short note on Hb-HHb buffer system
   • ii. Explain Sorensons reaction and formol titration pH of mixture of M acetic acid and 0.056 M sodium acetate (pKa =4.76)
   • iv. Explain titration curve of lysine
5. Q2 a) State True or False [4M]
   • iii. Colloids are always neutral of water is higher than oil
6. Q2 b) Attempt any three of the following
   • i. Explain significance of osmosis
   • ii. Explain affecting surface tension iil .What is morality & normality. Give equation
   • iv .Explain renal dialysis How is diffusion affected by distance?
   • vi . Explain precipitation of colloids
7. Q2 c) Attempt any two of the following {12M]
   • i. Explain donnan membrane equilibrium
   • ii . Elaborate on surface area and electric charge on colloids
   • iii .What do you mean by volume percentage. What is volume percentage if 25.5 grams of NaCl dissolved in 250 ml solution
   • iv .Calculate the ionic strength of 2MKCI solution
8. Q3 a) State True or False: [4M]
   • i. In compound microscope the orientation of the image you see is flipped in relation to the actual
   • ii. Diaphragm determines how much light falls on the specimen
   • iii. Phase contrast microscopy is valuable for visualizing yeast cells
   • iv. Image observed in the microscope will not appear upside down
9. Q3 b) Attempt any three of the following: [9M] i, State the difference between SEM and TEM microscopy ii Elaborate on the applications of Dark field and bright field microscopy iii, Write about the principle and significance of Confocal Microscopy
   • iv.Write about the different parts of a compound microscope. Elaborate any one
   • v. State the importance of condenser and diaphragm in light microscopy
   • vi. Write about TEM and its advantages
10. Q3 c) Attempt any two of the following:
    • i. Elaborate on Electron microscopy. Applications and advantages of SEM ii, Write a detailed account of Time lapse microscopy with its applications, advantages and
    • iii. Write an informative note simple florescence microscopy
    • iv. Elaborate on Phase Contrast microscopy
11. Q4 a) Define any five of the following [10M]
12. Q4 b) Attempt any three of the following
13. Q1 Isoelectric precipitation of casein
    • ii. product of water ili. How is surface tension measured?
    • iv. Role of bile in digestion
    • v. Write a detailed account of Confocal Scanning Laser Microscopy
    • vi. Write an elaborative note on DIC

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