BSc Biochemistry Sem III 2016 2017 2017 BIOCHEMISTRY 1 Question Paper - Mumbai University | munotes

Questions asked in this paper

1. Q1 A. Answer the following questions (any four) [8M]
   • 1) Who first demonstrated about enzyme? Who coined the term enzyme?
   • 2) What is the contribution of James Sumner in the field of enzymology?
   • 3) What is the chemical nature of an enzyme? Make a comment on the exception on this regard
   • 4) Define Kear
   • 5) What is holoenzyme?
   • 6) What do you mean by coenzyme? Give
   • 7) What is enzyme specificity? ) 8) What is transition state?
2. Q1 B. Answer the following questions. (Any two) [6M]
   • 1) What do you mean by Give the significance of Km
   • 2) How does substrate concentration affects rate of enzyme reaction?
   • 3) Write down Koshland’s induced fit theory of enzyme substrate interaction
   • 4) What do you mean by competitive inhibitor? Give the Lineweaver-Burk plot for competitive inhibitor
3. Q1 C. Answer the following questions in brief.(any one). [6M]
   • 1). Write a note on classification of enzyme according to enzyme commission
   • 2). Derive Michaelis-Menten equation for a single enzyme- substrate reaction
4. Q2 A Answer the following (any four) [8M]
   • 2) Other name of ADH
   • 3) Define steroid hormone
   • 4) What is exocrine gland ? Give example
   • 5) State true or False : Thyroid gland is known as the master gland
   • 6) Give example of peptide hormones
   • 7) Thyronine is a derivative of iodine
   • 8) Explain vasopressin in short
5. Q2 B Do as directed (Any Two) [6M]
   • 1) Give the physiological role of estrogens
   • 2) State the mode of action of steroid hormone
   • 3) State functions of ethylene
   • 4) Write down the structure of auxin and state any three functions
6. Q2 C Answer briefly ( Any one )
   • 1) State the physiological role of
   • 2) State the physiological role of glucocorticoids
7. Q3 A Answer the following (any four) (5M
   • 1) What is pH scale ?
   • 2) What is monoproticacid ?give one example
   • 3) What is pH scale
   • 4) Define physiological buffer
   • 6) What is pKa
   • 7) Give pK values of Glysine
   • 8) Define isoelectric pH
8. Q3 B directed (Any Two) [6M]
   • 1) Explain the ionization of nonpolar amino acid with suitable example
   • 2) Explain bicarbonate as a physiological buffer
   • 3) Derive ionic product of water
   • 4) - Explain the ionization of Glycine C Answer briefly ( Any one )
   • 1) Calculate the pH of a buffer solution prepared by dissolving 363 mg of Tris in 10 mL of 0.2M HCI and diluting to 100 mL with water [Tris: mw 121 g/mol and pKa = 8.08 for the conjugate acid
   • 2) Explain the ionization of Aspartic acid with suitable titration curve
9. Q4 1.A. Answer in short.(any one)
   • 1) Active site . Define Apoenzyme Fill up the blanks(any three)
   • 1) Unit of specific activity is .(Katal/ U/ pmole
   • 2) According to E.C number 6 denotes group of enzyme
   • 3) ATP, FAD are . (Cofactor/ Coenzyme/ Prosthetic
   • 4) Higher activation energy the reaction rate.(Slower/ Higher/ Moderate)
   • 5) Substrate binds to enzyme at . (catalytic site/ Binding site/ action site)
   • 6) Hydrolysis type of reaction catalysed by enzyme
10. Q4 2.A. Answer the following : (any 1) [2M]
    • 1) Classify hormones based on distance travelled
    • 2) Classify hormones based on chemistry
11. Q4 2.B. Match the following : (any 3) [ 3M]
    • 2. Pituitary gland b. T3 & T4
    • 4. Ovaries d. Oxytocin
    • 6. Root growth f
    • Q. Answer the following : (any [2M]
    • 1) Give one example of acidic amino acid with
    • 2) Give pl value of Glycine Fill in the blanks (any 3)
    • 1) Ammonium hydroxide — ammonium chloride is the example of buffer pK, value of Valine /10.5 ) is the simplest amino acid ( Proline lysine Glycine )
    • 4) Ifthe pH of Tris buffer is 12 then the ion concentration of bufferis If the pK value of molecule is will have a zero charge Conjugate base of

Read from the scan above, so a character or two may differ. The scan is the original.