BSc Biochemistry Sem IV ATKT 2018-19 ATKT SYBIO CHEM Biochemistry I Question Paper - Mumbai University | munotes

Questions asked in this paper

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1. Q1 A) Name the physicochemical property associated with the following scientists: 4 marks
2. Q1 B) Answer any three of the following: 9 marks
   • (i) Explain the applications of viscosity (i1) Define Ionic strength. Calculate the ionic strength of 0.5 M (i11)Discuss reverse osmosis and its applications
   • (iv) Write a note on protective colloid
   • (v) What is normality? Calculate the amount of potassium dichromate required to prepare 500 ml of 0.05 N solution for estimation of COD of water.(MW of potassium dichromate=294)
   • (vi) Differentiate between active and passive transport
3. Q1 C) Answer any two of the following: 12 marks
   • (i) Explain renal dialysis (i1) Give an account of properties of lyophilic and lyophobic sol (i11)Describe Donnan membrane equilibrium and explain its relation with osmotic pressure helps in digestion” — Justify. Elaborate on the factors which may affect the process
4. Q2 A) State True or False: 4 marks
   • (i) Liquid shear techniques use water as an abrasive material in order to break the microbial
   • (ii) C.elegans is a model organism for study of genetics of regeneration and healing
   • (iii) Phosphate buffer was designed to approximate ionic composition of mammalian serum
   • (iv). High temperature is used technique to separate cells
5. Q2 B) Answer any three of the following: 9 marks
   • (i) Justify the statement using examples: “Selection of suspending medium is crucial for
   • (ii) State TRUE or FALSE giving reasons: “Fishes cannot be used as a model organism”
   • (iii)Discuss in short giving examples on “Biochemical methods for shearing are better than
   • (iv) Briefly explain a technique that uses high pressure for cell disruption What is ‘Salt Fractionation’? Add a note on its applications
   • (vi) Give the advantages and disadvantages of Drosophila melanogaster as a model organism
6. Q2 C) Answer any two of the following: 12 marks
   • (i) With the help of a schematic representation depict path analysis of a biochemist’s approach to study exogenous compound
   • (ii). Give a detailed account of cell counting and sizing techniques ED29AE1247B4DC6F8A832BE3E86A 18CA
   • (iii) Discuss the role of the following in biochemical investigations: (a) Ultrasonicator
   • (iv) Write an elaborate note on different techniques of membrane separation
7. Q3 A) Match the following: 4 marks
   • e) Normal sunlight
   • f) Electron gun
8. Q3 B) State true or false, giving reasons: (Any three) 9 marks
   • (i) Higher numerical aperture, better is the resolution Phase contrast microscopy is useful for observing gram stained bacteria Red colour of light gives better resolution
   • (iv) Confocal microscopy is useful for live cell imaging
   • (v) Any sample slide can be observed by electron microscope
   • (vi) Fluorescence microscope has a special type of condenser
9. Q3 C) Answer any two of the following: 12 marks
   • (i) Elaborate on the similarities and dissimilarities between light and electron microscopy
   • (ii) Describe any two fluorescence microscopic techniques mentioning components and
   • (iii) Give comparative account of bright field, dark field and phase contrast microscopy
   • (iv) Discuss any four techniques used for sample preparation for electron microscopy
10. Q4 A) Define any five of the following: 10 marks
    • (v) (vi) Dynamic equilibrium (vii) Coefficient of viscosity
11. Q4 (B) Answer any three of the following: 15 marks
    • (i) Why immersion oil is necessary for 1000X but not with lower power objective? Would you use standard immersion oil on the plastic slide? Explain
    • (ii) List the properties of light. Explain how they are useful for observing small objects? an informative note on routes of administration in whole plant studies. Add a note on Arabidopsis as a model organism
    • (iv) Give a detailed account of use of radioactive compounds in biochemical studies
    • (v) Explain the factors affecting diffusion
    • (vi) Explain the principles of measurements of surface tension ED29AE1247B4DC6F8A832BE3E86A

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