BSc Biochemistry Sem IV 2018 19 Apr 2018-19 BIO CHEM BIOCHEMISTRY PAPER I Question Paper - Mumbai University | munotes

Questions asked in this paper

1. Q1 All questions are compulsory. Choice is internal
2. Q2 Figures to the right indicate full marks
3. Q3 Draw structures, diagrams or flowcharts wherever necessary
4. Q4 Normal calculator or log tables can be used if required
5. Q1 A) State true or false: 4 marks
6. Q1 Almost all enzymes are proteins
   • ii) NAD acts as a cofactor
   • iii) is a reversible inhibitor
   • iv) IU stands for Indian units of enzyme
7. Q1 B) Attempt any three of the following 9 marks
8. Q1 Differentiate between Fischer and Koshlands model of enzyme action
   • ii) The rate of an enzyme catalyzed reaction is 45 mol/min at [S] = M. Km for this substrate is 5 x M. Calculate the rate where [S]=2x mol
   • iii) Discuss the properties that differentiate an enzyme from a catalyst
   • iv) Describe the factors affecting enzyme activity
   • v) Write a note on active site of an enzyme
   • vi) With the help of a diagram explain how the activation energy of reaction is modulated in presence of an enzyme
9. Q1 C) Answer any two of the following 12 marks
10. Q1 Compare and contrast: Competitive and Noncompetitive inhibition of enzyme
    • ii) Derive an equation for Michaelis Menten constant for monosubstrate reaction ili) Give an account of enzyme specificity
    • iv) Graphically represent Lineweaver-Burk plot for enzyme catalysed reaction. Given an enzyme with a Km = 10 mM and Vmax = 100 mmol/min. Calculate Vo. If [S]=10mM, which will increase the velocity more: a 10-fold decrease in Km, or a 10 fold increase in Vmax?
11. Q2 A) Give an example of the following: 4 marks
12. Q1 The largest endocrine gland in the body
    • ii) Hormone helping reabsorption of water
    • iii) An aldosterone
    • iv) An auxin
13. Q2 B) Attempt any three of the following: 9 marks
    • i) Write a note on ‘Cytokinin’
    • ii) Distinguish between endocrine and exocrine secretion ili) Discuss the biochemical functions of progesterone
    • iv) Explain chemistry and functions of oxytocin
    • v) Describe various hormone receptors
    • vi) Write a short note on ethylene as a plant hormone
14. Q2 C) Answer any two of the following 12 marks
15. Q1 With the help of schematic representation explain amplification cascade in
    • ii) Write an informative note on chemistry and physiological role of thyroxine
    • iii) Give an elaborative account of physiological role of gibberellins
    • iv) Write a note on hormone
16. Q3 A) Match the following: 4 marks
    • iii) Scenedesmus c) cultivation of plant in nutrient medium
    • iv) Potassium chloride d) agglutination of liver homogenate
    • e) tracer for protein synthesis
    • f) assessment of viability of cells
17. Q3 B) Attempt any three of the following: 9 marks
18. Q1 Explain the working of a French Press
    • ii) State the disadvantages involved in perfusion of isolated organs ili) Write in brief about the different techniques adopted for isolation of individual cells from the tissue
    • iv) Give a brief account on any two techniques of membrane separation
    • v) Elaborate on the importance of enzymes in biochemical investigations
    • vi) Write advantages and disadvantages of Arabidopsis as model organisms Answer any two of the following: 12
19. Q1 Write a detailed account of any one equipment used for determination of cell size and
    • ii) With the help of a flow chart describe the steps involved in isolation of different cell ili) Write a short note on whole plant studies. Also give examples of the plants used for
    • iv) Write an elaborative note on C. elegans and Dictyostellium as model organism Define and explain any five terms: 10
20. Q4 B) Attempt any three of the following: 15 marks
21. Q1 Explain IUB classification of enzymes in detail li) Define coenzyme. Give an account of vitamins and their coenzyme forms
    • iii) Explain mode of action of steroid hormone
    • iv) Give an account of Abscisic acid as plant growth regulator
    • v) Draw a diagram representing path analysis of a biochemist’s approach to study
    • vi) Give an account of cell counting

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