BSc Biochemistry Sem IV 2015 16 2015-16 BIOCHEM 1 Question Paper - Mumbai University | munotes

Questions asked in this paper

1. Q1 questions in one sentence. (any 4) 8 marks
2. Q2 How is viscosity affected by molecular conformation?
3. Q3 What is diffusion pressure?
4. Q4 What is the relation of concentration and osmotic pressure? of ions against concentration gradient?
5. Q7 What is osmolarity?
6. Q8 Explain Endosmosis and Exosmosis
   • B) Answer the following in brief. (Any 2) 6 Define mole. Explain molarity (
7. Q2 Explain viscosity
8. Q3 Write a note on mechanism of osmosis
9. Q4 . Describe colloidal state
   • C) Answer the following in detail. (Any 1) 6 1). Explain Donan’s Equilibrium with example
10. Q2 Give any two methods of measuring viscosity Answer the following in one sentence. (Any 8
11. Q2 State the use of collagenase
12. Q3 What is microdissection?
13. Q4 an isotonic solution?
14. Q5 Give two examples of chemicals used for cell disruption
15. Q6 What is the significance of paraffin wax in tissue studies?
16. Q7 Give the name of the plant used as a model organism
    • B) Answer the following in brief. (Any 2) 6 Writea short note on C. elegans as a model organism
17. Q2 Describe coulter counter
18. Q3 Explain the use of mouse as an exper imental model
19. Q4 Whatisliquid shear? 6 marks
    • C) Answer the following in detail. (Any
20. Q1 Give an account of Centrifugation as a tool for cell fractionation
21. Q2 Give an account of E. Coli as.a model organism Answer the followings in one sentence. (Any
22. Q1 What is the source of light In compound
23. Q2 Whatis the function of a mirror In
24. Q3 What is the function of fine focus
25. Q4 Which:objectives are known as Wet objectives?
26. Q5 resolving power?
27. Q6 What is Fluoroscence?
28. Q7 What is a barrier filter?
29. Q8 What is Numerical aperture?
    • B) Answer the following in brief. (Any 2) 6 Draw ray diagram of Fluorescence microscope
30. Q2 Draw ray diagram of phase contrast microscope
31. Q3 Give the advantages of TEM
32. Q4 Give the disadvantages of light microscope Answer the following questions in detail. (Any 1)
33. Q1 Give the working of SEM
34. Q2 Give the working of light microscope
    • A) Explain the followings. (Any 1)
35. Q1 Dialysis Match the following. (Any 3) : 1) Diffusion pressure a) Movement of solvent
36. Q2 b) Shear force A
37. Q5 Osmotic pressure e) Pressure required to stop osmosis
38. Q6 Dialysis Force generated by diffusion : Explain the following. | 2 2). Tissue embedding Fill in the blanks. (Any 3) 3
39. Q1 are capable of bringing some hydrophobic compounds into aqueous solutions. )
40. Q2 is an appliance that can count cells as well as measures their volume
41. Q3 Cell cultures prepared directly from the tissues of an organism are called as
42. Q4 The has been a crucial model organism in developmental biology
    • (E.coli Drosophila)
43. Q5 When centrifuged, the various components in a mixture move as a series of distinct bands through the salt solution, each at a different a process called
44. Q6 employs high frequency sound for cell disruption
    • E) Write short note on: (Any1)
45. Q1 Application of phase contrast microscope
46. Q2 Application of Fluorescence
    • F) Fill in the blanks. (Any 3) 3
47. Q1 The resolving power is limited by illuminating The direction and magnitude of bending of light is determined by two media forming the interface. (Refractive indices Refraction The distance between the center of the lens and the focal pointis called The ability of to gather light is known used as-an intense beam of light of epifluorescence microscope
48. Q6 Thin slices of sample embedded in solid epoxyplastic is cut by using 3 marks

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