B.Sc. (Biochemistry) Sem III 2022 2023 Oct 2023 BIOCHEMISTRY I Question Paper - Mumbai University | munotes

Questions asked in this paper

• 2. Figures to the right indicate full marks

1. Q1 A) State True or false: 4 marks
   • (i) pl is used in protein separation using PAGE (it) Weak acid are proton donor
   • (iii) Buffers cannot resist the change in pH
   • (iv) pl stands for isoelectric permeability
2. Q1 B) Write short notes on: (Any three) 9 marks
   • (i) Formulas used for calculating pH and pOH
   • (ii) pK
   • (iii) Titration curve of lysine
   • (v) pH and pOH
   • (vi) pKa, and
3. Q1 C) Answer of the following: (Any two)
   • (i) Explain what are buffers, buffering action and isoelectric point in detail
   • (ii) Explain any two buffer system in detail
   • (iii) Describe in detail about Henderson —Hasselbalch equation
   • (iv) Explain in brief the titration curve of glycine
4. Q2 A) State True or False: 4 marks
   • (i) Colloids do not precipitate on addition of electrolyte
   • (ii) Viscosity of sucrose solution is less than water
   • (iv) Colloidal solutions have higher surface area than suspension solutions
5. Q2 B) Answer the following: (Any three) 9 marks
   • (i) Elaborate on surface area of colloids
   • (ii) Explain the tyndall effect with an example
   • (iii) Describe role of bile in digestion
   • (iv) Explain flocculation of colloids
   • (v) What is the volume percentage when 63 grams of NaCl is dissolved in 250 ml water?
   • (vi) Describe renal dialysis
6. Q2 C) Answer the following: (Any two) 12 marks
   • (i) Calculate the ionic strength of 1M HCI solution
   • (ii) Explain the mechanism of osmosis
   • (iii) Methods to measure surface tension
   • (iv) Elaborate on properties of colloids VCD OF Sem III Biochemistry
7. Q3 A) State True or False: 4 marks
   • (i) Anthony Leeuwenhoek is called the "Father of Microscopy"
   • (ii) In dark field microscope, the slide, stage, nose and light source should be free from small particles such as dust
   • (iii)Nomarski Prism is also known as a Winston prism or condenser prism
   • (iv)A rotating turret that houses the objective lenses is called as eyepiece
8. Q3 B) Write short notes on: (Any three) 9 marks
   • (i) Explain simple microscope
   • (ii) Prism used in difference interference contrast
   • (v) Applications of microscope
9. Q3 C) Answer the following: (Any two) 12 marks
   • (i) Explain fluorescence microscopy in detail
   • (ii) With the help of ray diagram explain differential interference contrast
   • (iii) Explain the advantages and of dark field microscope
   • (iv) With the help of ray diagram phase contrast microscope
10. Q4 A) Define and explain: (Any five) 10 marks
11. Q4 B) Write short notes on: (Any three) (145)
    • (i) Write a short note on titration curve of aspartate
    • (ii) Explain in detail the buffering system of bicarbonate
    • (iii) Explain the importance of surface tension in the role of bile acid in digestion
    • (iv) Enlist the importance of osmosis in physiology
    • (v) With the help of diagram explain dark field microscopy
    • (vi) Write in brief about the history of microscope

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