BSc Sem VI 2022 2023 Apr 2023 BIOCHEMISTRY I METABOLISM AND ANALYTICAL TECHNIQUESN II Question Paper - Mumbai University | munotes

Questions asked in this paper

1. Q1 All the questions are compulsory. Choice is internal
2. Q2 Figures to the right indicate full marks
3. Q3 All questions carry equal marks
4. Q5 Use of simple calculator is permitted
5. Q1 A) the blanks: 4 marks
6. Q1 Activation of fatty acids require breakdown of ATP to
   • ii) Acyl-CoA is transferred to the hydroxyl group of carnitine by
   • iii) Acetone, acetoacetate and are considered as ketone
   • iv) Oxidation of a fatty acid containing 18 carbon atoms to acetyl CoA would require number of cycles
7. Q1 B) Write a note on (ANY ONE) 4 marks
8. Q1 Beta oxidation of odd carbon chain fatty acids Answer ANY TWO 12
9. Q1 Compare the activation steps of beta-oxidation and lipogenesis. Elaborate the same with detailed chemical reactions
   • ii) Justify- The liver cannot utilise ketone bodies. Add a note on the significance of ketone bodies in starvation and alcoholism ili) Calculate the total number of ATPs generated from the stearic acid
10. Q2 A) the blanks 4 marks
11. Q1 The coenzyme used in transamination reaction Decarboxylation of histidine produces lil) is an intracellular receptor
    • iv) Epinephrine receptor on the cell membrane contains
12. Q2 B) Write a note on ANY ONE 4 marks
13. Q1 Hormone receptors li) Ketogenic and glucogenic amino acids
14. Q2 C) Answer ANY TWO 12 marks
15. Q1 Compare oxidative and non-oxidative deamination
    • ii) Discuss in detail the contributions of Kurt Henseliet and Hans Kreb to the field of protein metabolism
    • iii) With reference to signal transduction comment on
16. Q3 A) the blanks 4 marks
    • i) Wavelength selectors are of two types, filter and
    • ii) Centrifugal field with respect to gravity is known as
    • iii) Unit of sedimentation coefficient is
    • iv) Amino acids are estimated in colourimeter by reacting with as Compare ANY ONE 4
17. Q1 Preparative and Analytical ultracentrifuge
    • ii) Detectors in colorimeter and spectrophotometer
18. Q3 C) Attempt any TWO 12 marks
    • i) Derive an expression for Beer Lambert’s law. Calculate the molar extinction coefficient of a solution containing 3M of a given substance which absorbs 80% incident light of wavelength 340 nm when the path length is 1 cm is asked to prepare a write-up for gradient material used in density gradient centrifugation. Help her collate the data with the aid of following
    • a) Ideal properties of gradient materials
    • b) Two examples of gradient material
    • iii) Elaborate on the colorimeter with reference to
    • (a) Source of light (b) Wavelength selector (c) Sample holder Fillin the blanks 4
19. Q1 Increase in temperature the rate of migration of analytes
    • ii) The gas produced at the anode during an electrophoretic run is ili) radiation is used for food preservation
    • iv) In GM counter the outer chamber wall serves as electrode
20. Q4 B) short note on ANY ONE 4 marks
21. Q1 Agarose electrophoresis for the separation of DNA
    • i) Isoelectric focusing
22. Q4 C) Attempt any TWO 12 marks
23. Q1 Defend- Selecting the appropriate support medium, buffer and electric field strength is crucial for electrophoresis
    • ii) Deriving an expression, explain how the radioactive decay constant is associated with half-life
    • iii) Compare:
    • (a) Moving boundary and zone electrophoresis
    • (b) Beta and Gamma radiations
24. Q5 A) Define 8 marks
    • a) Lipogenesis
    • b) Beta oxidation
    • c) cAMP
    • d) Ornithine
    • g) Radioactivity
    • h) Southern blotting
25. Q5 B) State True or False with justification 12 marks
26. Q1 Oxidation of fatty acids takes place in mitochondrial matrix
    • ii) The atomic mass number is denoted by
    • iii) RCF and rpm are not related to each other
    • iv) Lambda max is independent of concentration for a_ particular
    • v) Svedberg (S) is the unit of absorbance
    • vi) pH of the separating gel is 2 units lower than that of stacking gel

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