B.Sc. (Biotechnology) Microbial Techniques Syllabus - Mumbai University
This is the FY BSc Biotechnology syllabus under NEP 2020, in force from the academic year 2023-24. The University still examines the earlier Choice Based papers alongside it — her third-year office registers name that scheme — so check which scheme your exam form names before you revise.
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Syllabus for Microbial Techniques
1
- 1 Good laboratory practices in microbiology laboratory and Biosafety measures.
- 2 Enumeration of microorganisms by Breed’s count method/using counting chambers.
- 3 Observation of cell motility in different microorganisms - bacteria, algae, paramecium by wet mount method/hanging drop method /swarming growth method.
- 4 Staining of spirochetes from tooth tartar.
- 5 Special staining: Endospores staining/Capsule Staining/cell wall staining
- 6 Study of the effect of different A) Physical factors like pH, temperature B) Chemical factors like Sodium Chloride, heavy metals (oligodynamic action) on growth of E.coli.
- 7 Qualitative detection of microbial enzymes like catalase, amylase, protease, lipase, dehydrogenase.
- 8 Enrichment and isolation of halophilic bacteria from marine environments.
- 9 Study of the effect of washing skin with soap and disinfectants on its microflora.
- 10 Isolation of different fungal species from spoiled fruit/vegetable samples.
- 11 Comparative microscopic study of microflora from different fermented foods.
Text Books
- 1 Textbook of Biochemistry with Clinical Correlations, 7th Edition, Thomas M. Devlin, January 2010,
- 2 Biosafety in Microbiological and Biomedical Laboratories-CDC
- 3 LABORATORY BIOSAFETY MANUAL FOURTH EDITION-WHO
- 1 Basic Practical Microbiology A manual, Published by the Society for General Microbiology, Marlborough House, Basingstoke Road, Spencers Wood, Reading RG7 1AG, UK
- 2 Forbes B. A, et al: Baily and Scotts: Diagnostic Microbiology 12th Edition.
- 3 Ochei J and Kolhatkar (2000); Medical Laboratory Science, Theory and Practice, Tata McGraw-Hill Publishing Company
- 4 Biochemical Methods.1st , (1995), S. Sadashivam, A.Manickam, New Age International Publishers, India
Reproduced from the University of Mumbai syllabus for B.Sc. (Biotechnology) under NEP 2020, in force from the academic year 2023-24. Wording is as printed in that syllabus. Module numbering is as printed there too.