B.E. (Chemical Engineering) Biotechnology Lab Syllabus - Mumbai University 2026
The University has moved this degree onto NEP 2020 one year at a time. The first, second and third years are on NEP 2020; the final year is still on the REV-2019 'C' Scheme, which is what the University sets for it this year.
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Syllabus for Biotechnology Lab
Module 1: To estimate the concentration of glucose in a given
- sample using the DNSA colorimetric method.
Module 2: To determine the amount of DNA present in a biological
- sample using the diphenylamine (DPA) assay.
Module 3: To estimate the RNA content of a sample using the orcinol
- colorimetric method.
Module 4: To quantify the protein concentration in a sample using
- either the Lowry method or the Bradford assay.
Module 5: To determine the optimum pH at which a given enzyme
- exhibits maximum activity.
Module 6: To determine the optimum temperature for maximum
- enzymatic activity.
Module 7: To calculate the Vmax and Km values of an enzyme using
- Michaelis Menten kinetics.
Module 8: To study the effect of different inhibitors on enzyme
- activity and identify the type of inhibition.
Module 9: To create a simplified process flow diagram (PFD)
- illustrating the upstream processing, purification, and formulation steps involved in vaccine or monoclonal antibody production
Module 10: To study major biological databases and understand their
- applications in storing, retrieving, and analyzing biological information.
Module 11: To perform biological sequence alignment using
- bioinformatics tools and interpret the alignment results.
Module 12: To prepare a case study demonstrating the applications of
- biotechnology in agriculture, food and beverage, chemical industries, environmental management, or bioenergy.
Module 13: To precipitate proteins from a sample using ammonium
- sulfate (salting-out method).
Module 14: To perform dialysis to remove small molecules from a
- protein or dye solution using a semipermeable membrane.
Module 15: To separate amino acids using paper chromatography and
- analyze their migration patterns.
Reproduced from the University of Mumbai syllabus for B.E. (Chemical Engineering) under NEP 2020, in force from the academic year 2026-27. Wording is as printed in that syllabus. Module numbering is as printed there too.
The complete syllabus
This subject is cut from the University circular for its year. Open a document here if you want the whole thing rather than a single subject.