B.Sc. (Biotechnology) Practicals in Molecular Biology Syllabus - Mumbai University 2026
This is the SY BSc Biotechnology syllabus under NEP 2020, in force from the academic year 2025-26. The University still examines the earlier Choice Based papers alongside it — her third-year office registers name that scheme — so check which scheme your exam form names before you revise.
Loading syllabus...
Syllabus for Practicals in Molecular Biology
- 1 Isolation of genomic DNA from plant cells.
- 2 Isolation of genomic DNA from bacterial cells.
- 3 Comparative genomic DNA profiling from different organisms using AGE.
- 4 Isolation and detection of RNA from bacteria or yeast.
- 5 Purity assessment of extracted RNA samples using spectrophotometry.
- 6 Purity assessment of extracted DNA samples using spectrophotometry.
- 7 Protein extraction from plant cells.
- 8 Protein extraction from bacterial cells.
- 9 Separation of extracted proteins using PAGE.
- 10 DNA Amplification – PCR. (Demonstration0
- 11 Demonstration of protein-DNA interactions through gel shift assays. is this doable
- 12 Study of lac gene expression using blue-white selection.
- 13 DNA microarray - Demonstration.
- 14 Chromatin immunoprecipitation - Demonstration.
- 15 Virtual lab for transcription and translation - https://learn.genetics.utah.edu/content/basics/transcribe.
Text Books
- 1 Sambrook, J., & Russell, D. W. (2001). Molecular cloning: A laboratory manual (3rd ed.). Cold Spring Harbor Laboratory Press.
- 2 Brown, T.A. (2016). Gene Cloning and DNA Analysis: An Introduction. 7th ed. Chichester: John Wiley & Sons Ltd.
- 3 Green, M.R. & Sambrook, J. (2012). Molecular Cloning: A Laboratory Manual (Fourth Edition). Cold Spring Harbor, NY: Cold Spring Harbor Laboratory Press.
- 4 Primrose, S.B. & Twyman, R. (2006). Principles of Gene Manipulation and Genomics. 7th Edition. Chichester: Wiley-Blackwell. ISBN: 978-1-405-13544-3.
- 5 Dieffenbach, C.W. & D'Veksler, G.S. (2003). PCR Primer: A Laboratory Manual. 2nd ed. Cold Spring Harbor Laboratory Press.
Reproduced from the University of Mumbai syllabus for B.Sc. (Biotechnology) under NEP 2020, in force from the academic year 2025-26. Wording is as printed in that syllabus. Module numbering is as printed there too.