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B.Sc. (Biotechnology) Practicals Syllabus - Mumbai University 2026

This is the FY BSc Biotechnology syllabus under NEP 2020, in force from the academic year 2023-24. The University still examines the earlier Choice Based papers alongside it — her third-year office registers name that scheme — so check which scheme your exam form names before you revise.

Practicals Syllabus.pdf
Major · Semester 2 · FY BSc Biotechnology · 2 credits · 50 marks

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Syllabus for Practicals

Major · Semester 2 · FY BSc Biotechnology · 2 credits · 50 marks

Module I

  • 1 Study of mitosis from suitable plant material
  • 2 Study of meiosis from suitable plant material/Permanent slides/Photographs
  • 3 Study the effect of disinfectants on fomite surfaces.
  • 4 Extraction and isolation of genomic DNA from various plant materials.
  • 5 Purity and estimation of extracted DNA and RNA using UV-Vis Spectroscopy
  • 6 Quantitative estimation of DNA
  • 7 Quantitative estimation of RNA
  • 8 Study of Karyotype - Normal male, Normal female, Down Syndrome, Klinefelter’s Syndrome and Turner’s Syndrome
  • 9 Barr body identification in cells of Buccal smear.
  • 10 Problems based on Mendelian Genetics, its modifications and gene interactions.
  • 11 Construction of pedigree charts and analysis of Human genetic traits using Pedigree
  • analysis.
  • 12 Extraction and isolation of Genomic DNA from E. coli.
  • 13 Separation and visualisation of DNA by Agarose gel electrophoresis (Demo)
  • 14 Basic problems on Restriction Digestion Mapping
  • 15 Study of ABO Blood groups in humans to understand the concept of multiple alleles using data collection strategies.
  • 16 Enzymes in Action: Exploring the Role of Restriction Endonucleases in Genetic Engineering(Assignment)
  • 17 Role of GMO’s in controlling Environmental pollutions(Assignment)

Text Books

  • 1 Principles of Genetics, 7th Edition D. Peter Snustad, Michael J. Simmons Publisher Wiley
  • 2 Principles Of Genetics by Gardner E.J Publisher Wiley India
  • 1 Gene Cloning & DNA Analysis: An Introduction T A Brown Publisher Wiley- Blackwell

Reproduced from the University of Mumbai syllabus for B.Sc. (Biotechnology) under NEP 2020, in force from the academic year 2023-24. Wording is as printed in that syllabus. Module numbering is as printed there too.

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