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B.Sc. (Biotechnology) Fundamentals of Biotechnology II Syllabus - Mumbai University 2026

This is the FY BSc Biotechnology syllabus under NEP 2020, in force from the academic year 2023-24. The University still examines the earlier Choice Based papers alongside it — her third-year office registers name that scheme — so check which scheme your exam form names before you revise.

Fundamentals of biotechnology - II Syllabus.pdf
Major · Semester 2 · FY BSc Biotechnology · 2 credits · 50 marks

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Syllabus for Fundamentals of biotechnology - II

Major · Semester 2 · FY BSc Biotechnology · 2 credits · 50 marks

Module I: Introduction to Genetic Engineering

  • 1 What is Genetic engineering: Definition and developments, gene cloning, Steps for cloning (2 Lectures)
  • 2 Enzymes in genetic engineering: Restriction endonuclease; DNA ligase; Enzymes to modify ends of DNA molecules - exonuclease; endonuclease; S1 nuclease; alkaline phosphatase; polynucleotide kinase; DNA polymerase and klenow fragment; reverse transcriptase; terminal deoxynucleotidyl transferase (3
  • Lectures)
  • 3 Vectors: Role as agents of transfer, Features of plasmid vectors, Plasmid vectors - pBR322, pUC etc. (4 Lectures)
  • 4 Host cells: E. coli; Bacillus subtilis; Saccharomyces cerevisiae; Xenopus oocytes; Mammalian fertilized egg cell (3 Lectures)
  • 5 Introducing vector into host: Prokaryote, Eukaryote, Identification of recombinant clones. (3 Lectures)

Module II: Applications of biotechnology

  • 1 Agriculture: GM fruits- GM papaya, GM tomato, Insect resistant transgenic plants – Bt cotton, Bt brinjal, Modifications in nutrient quality – starch, oilseed protein,
  • golden rice (4 Lectures)
  • 2 Animal Biotechnology: Growth, disease resistance, product quality, pharmaceuticals and nutritional supplements, industrial applications
  • Lectures)
  • 3 Human welfare: Cloned genes for production of -Insulin; recombinant vaccine for Hepatitis B virus. Molecular farming, Edible vaccines and their advantages
  • Lectures)
  • 4 Environment Pollution: Role of Biotechnology in control of pollution
  • (2 Lectures)

Text Books

  • 1 Dubey, R. C. (1993). A textbook of Biotechnology. S. Chand Publishing.
  • 2 Dubey, R. C. (2014). Advanced biotechnology. S. Chand Publishing.
  • 3 Singh, B. D., & Singh, B. D. (2007). Biotechnology expanding horizons. Kalyani publishers.
  • 1 Nicholl, D. S. T. (2002). An Introduction to Genetic Engineering (Studies in Biology). India: Cambridge University Press.
  • 2 Brown, T. A. (2013). Gene Cloning and DNA Analysis: An Introduction. Germany: Wiley.
  • 3 Genetic Engineering: Principles and Practice. (n.d.). India: McGraw-Hill Education.
  • 4 Principles of Gene Manipulation and Genomics - Richard M Twyman and S. B. Primrose
  • 5 Molecular Biotechnology - Principles and Applications of Recombinant DNA - Bernard R. Glick, Jack J. Pasternak

Reproduced from the University of Mumbai syllabus for B.Sc. (Biotechnology) under NEP 2020, in force from the academic year 2023-24. Wording is as printed in that syllabus. Module numbering is as printed there too.

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