MSc Biotechnology SEM I 2023 2024 Feb 2024 MOLECULAR DIAGNOSTICS Question Paper - Mumbai University | munotes

Questions asked in this paper

• 1. Attempt all questions

1. Q3 Use of log tables and non-programmable calculators is allowed
2. Q1 the following: (Any One) 1 Molecular amplification techniques- Any two types 2 Microarrays- Construction, types and applications account of the following: (Any One) 07 1 Direct detection and identification of tuberculosis 2 Mass spectrometry —Instrumentation and applications. Add a note on Principle 8 marks
3. Q3 directed 1 Fill in the blank: Molecular identification methods based on the extraction of total fungal DNA offer a distinctive that can be used to determine and identify various fungal isolates up to the species level 2 State True or False: Absolute quantification determines expression levels in absolute numbers of copies 3 Fill in the blank: To identify exon-level copy number variation amplification method is used 4 State True or False: The polymerase chain reaction consists of four steps: denaturation, annealing, hybridization, and extension 5 Fill in the blank: Historically due to its high conservation, sequencing is used as a marker for identification of pathogens 6 Give the reagent used in Edman degradation of proteins 7 Fill in the blank: technique in study of gene expression uses mRNA from a particular sample to create complementary DNA (cDNA) fragments which are then amplified and sequenced using high-throughput 8 Give the definition of protein microarray 9 Give any one example of commercial NGS platform 10 List the two dimensions in 2D PAGE 10 marks

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