B.Sc. (Biotechnology) Microbial Laboratory Techniques Syllabus - Mumbai University 2026

• 1 Isolation of fungi from different sources.
• 2 Preservation of microbial cultures by various techniques.
• 3 Microbial examination of sterile and non sterile products.
• 4 Replica plate technique to isolate drug resistant mutants.
• 5 Gradient plate technique to isolate drug/dye resistant mutants..
• 6 MPN estimation - [Presumptive, Confirmed and Completed] of bacteria in liquid culture/water.
• 7 Slide culture of Streptomyces.
• 8 Microbial methods of determination of Penicillin/ Streptomycin.
• 9 Enrichment and isolation of Rhizobium species on Yeast mannitol agar (YMA)
• 10 Single cell protein Biomass production at flask level (Yeast/ Spirulina)
• 11 Estimation of invertase enzyme activity by immobilization of yeast.
• 12 Pigment production from any one fungus
• 13 Workshop/ seminar with industrial collaboration on interactions with Entrepreneurs in Microbial Biotechnology and startups.

Text Books

• 1 Alexopoulus, C.J., Mims,C.W. and Blackwell, M, Introductory Mycology. John Wiley, New York.
• 2 Microbiology - A Laboratory Manual - James Cappuccino and Natalie Sherman.
• 3 A Textbook of Pharmaceutical Microbiology Paperback (2018) by Pulak Mujumder, Sameer Rajan Sahoo Everest Publishing
• 4 Practical Microbiology by R C Dubey and D K Maheshwari.2002. Chand Publications.
• 5 BlackJ.G.Microbiology-Principles Explorations.JohnWiley&SonsInc NewYork,(2002).
• 6 Tom Besty,D.C Jim Koegh.Microbiology Demystified McGRAW-HILL.
• 7 Madigan,Martinko, Bender,Buckley,Stahl.Brock Biology of Microorganisms. Pearson.
• 8 H.D. Kumar and H.N. Singh.A Textbook on Algae (Macmillan international college edition)
• 1 Mehrotra, R.S. and K.R.Aneja An Introduction to Mycology. New Age International A. V. S. S. .Sambamurty. A Textbook of Algae. I.K. International Publishing House Pvt. Limited, 2010.
• 2 Richard H. Baltz. Julian E Davies and Arnold L.Demain Manual of Industrial Microbiology and Biotechnology. 3rd edition, ASM Press (2010).