B.Sc. (Biotechnology) Practical in Industrial Biotechnology Syllabus - Mumbai University 2026

• 1 Screening of microorganisms from natural sources for their industrial potential.
• a Crowded plate technique for primary screening of antibiotics producing microorganisms from soil.
• b Wilkins overlay method for and secondary screening and identification of antibiotics producing microorganisms from soil.
• c Auxanography of growth factors
• 2 Cellulase production by Aspergillus niger:
• a Enzyme activity assay,
• b Optimization parameters of and
• c Yield comparison between surface / submerged cultures.
• 3 Preparation of growth media used in industrial fermentations.
• 4 Optimization of culture media for industrial microbial cultures.(Any one media parameter - Carbon /Nitrogen)
• 5 Inoculum preparation fungal culture (yeast / mold) for inoculation into a fermentor.
• 6 Comparison of shaker flask vs static bioreactor conditions.
• 7 Extraction of alginate biopolymer from Azotobacter species.
• 8 Gradient plate technique
• 9 Isolation of Antibiotic resistant organisms using replica plate technique.
• 10 Isolation of industrially important enzymes (amylase) producing micro- organisms from environmental samples.
• 11 Lab scale production of ethanol using S. cerevisiae - monitoring fermentation parameters like product formation, biomass etc.
• 12 Estimation of percentage of alcohol present in the recovery broth by dichromate method.
• 13 Recovery of ethanol from fermented broth by distillation.
• 14 Determination of total biomass present in the fermentation broth by wet weight and dry weight method.

Textbooks

• 1 Aneja, K. R. (2014). Experiments in Microbiology, Plant Pathology and Biotechnology (4th ed.). New Delhi: New Age International Publishers.
• 2 Bailey, J. E., & Ollis, D. F. (2010). Biochemical Engineering Fundamentals (2nd ed.). New York: McGraw-Hill.
• 1 Cappuccino, J. G., & Sherman, N. (2018). Microbiology: A Laboratory Manual (11th ed.). Pearson Education.
• 2 Dubey, R. C., & Maheshwari, D. K. (2011). Practical Microbiology. New Delhi: S. Chand & Company Ltd.
• 3 Waksman, S. A. (1967). The Actinomycetes: A Summary of Current Knowledge. New York: Ronald Press.
• 4 American Public Health Association (APHA). (2017). Standard Methods for the Examination of Water and Wastewater (23rd ed.). Washington, DC: APHA.
• 5 Central Public Health and Environmental Engineering Organisation (CPHEEO). (2013). Manual on Sewerage and Sewage Treatment. Ministry of Housing and Urban Affairs, Government of India.
• 6 Department of Biotechnology (DBT), Government of India. (2022). Indian Biotechnology Industry Report. New Delhi: DBT.