B.Sc. (Biotechnology) Practical in Genomics and Proteomics Syllabus - Mumbai University 2026
This is the TY BSc Biotechnology syllabus under NEP 2020, in force from the academic year 2026-27. The University still examines the earlier Choice Based papers alongside it — her third-year office registers name that scheme — so check which scheme your exam form names before you revise.
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Syllabus for Practical in Genomics and Proteomics
- 1 Genomic DNA Isolation: Animal cells.
- 2 Isolation of plasmid from E. coli
- 3 Qualitative analysis of isolated genomic DNA using AGE.
- 4 Qualitative analysis of an isolated plasmid using AGE.
- 5 Transformation in E. coli.
- 6 Real-Time qPCR- Working Principle and data analysis (Demonstration)
- 7 Protein extraction from biological samples (Plant/Animal/Bacterial)
- 8 Protein estimation (Lowry method)
- 9 Determine the viscosity of protein by using a viscometer.
- 10 Protein purity analysis using PAGE.
- 11 2-D gel electrophoresis (Demonstration)
- 12 Protein Secondary Structure Prediction Using PSIPRED
- 13 AI-Based Prediction of Protein Tertiary Structure Using AlphaFold.
- 14 AI-Based Variant Calling Using DeepVariant.
- 15 Assignment on a case study on proteomics-based disease diagnosis
Textbooks
- 1 Molecular Cloning: A Laboratory Manual (Fourth Edition), Michael R. Green, Joseph Sambrook.
- 2 Lovrien, R. Proteomics: Methods and Protocols, Humana Press
- 3 Srivastava, S. (2023). From Proteins to Proteomics: Basic Concepts, Techniques, and Applications. 11 Weblinks :
- 1 https://www.bio-rad.com/webroot/web/pdf/lsr/literature/Bulletin_5279.pdf
- 2 IDT Real-time PCR Handbook (RUO22-0835_001 09/23)
Reproduced from the University of Mumbai syllabus for B.Sc. (Biotechnology) under NEP 2020, in force from the academic year 2026-27. Wording is as printed in that syllabus. Module numbering is as printed there too.