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B.Sc. (Biotechnology) Practicals in Biochemistry Syllabus - Mumbai University

This is the SY BSc Biotechnology syllabus under NEP 2020, in force from the academic year 2025-26. The University still examines the earlier Choice Based papers alongside it — her third-year office registers name that scheme — so check which scheme your exam form names before you revise.

Practicals in Biochemistry Syllabus.pdf
Major · Semester 4 · SY BSc Biotechnology · 2 credits · 50 marks

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Syllabus for Practicals in Biochemistry

Major · Semester 4 · SY BSc Biotechnology · 2 credits · 50 marks

  • 1 Estimation of Reducing sugar by DNSA method
  • 2 Extraction of enzyme plant/Animal and detection of its activity (e.g Amylase/ Proteolytic )
  • 3 Study of the effect of pH on 𝛃 amylase activity
  • 4 Study of the effect of Temperature on 𝛃 amylase activity
  • 5 Study of the effect of Inhibitors on 𝛃 amylase activity
  • 6 Study of Effect of Substrate Concentration on enzyme activity and determination of Vmax and Km
  • 7 Isolation of Mitochondria and Demonstration of ETC using a Marker Enzyme.
  • 8 Separation of fatty acids by TLC.
  • 9 Meat tenderization using papain.
  • 10 Study of titration curve of amino acids.
  • 11 Activity of Salivary Amylase on Starch
  • 12 Immobilization of enzymes by entrapment method.
  • 13 Assignment- The application of enzymes in the production of active pharmaceutical ingredients (APIs), drug synthesis, and how they help
  • improve efficiency and sustainability in the pharmaceutical industry.
  • 14 Assignment- Cancer cell metabolism-key altered enzymes.

Text Books

  • 1 Biochemical methods, 4th edition, S.Sadasivam and A.Manickam, New Age International Publishers.
  • 1 An Introduction to Practical Biochemistry, 3rd edition, David Plummer, TATA McGrawHill Edition.
  • 2 Experimental Biochemistry: A Student Companion (2005), Beedu Shashidhar Rao and Vijay Deshpande, New Delhi: I.K. International. Introductory Practical Biochemistry, S.K. Sawhney and Randhir Singh, Narosa Publishing House.

Reproduced from the University of Mumbai syllabus for B.Sc. (Biotechnology) under NEP 2020, in force from the academic year 2025-26. Wording is as printed in that syllabus. Module numbering is as printed there too.

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