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B.Sc. (Biotechnology) Introduction to Bioinformatics Syllabus - Mumbai University 2026

This is the SY BSc Biotechnology syllabus under NEP 2020, in force from the academic year 2025-26. The University still examines the earlier Choice Based papers alongside it — her third-year office registers name that scheme — so check which scheme your exam form names before you revise.

Introduction to Bioinformatics Syllabus.pdf
Major · Semester 3 · SY BSc Biotechnology · 2 credits · 50 marks

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Syllabus for Introduction to Bioinformatics

Major · Semester 3 · SY BSc Biotechnology · 2 credits · 50 marks

Biological Databases and Analysis Tools

  • 1 Introduction to Biological Databases: GenBank, EMBL, PDB, UniProt, Ensembl.
  • 2 Retrieve a sequence from Databases: Retrieve a DNA sequence from GenBank.
  • 3 Retrieve a sequence from Databases: Retrieve a Protein sequence from UniProt, PDB.
  • 4 Sequence alignment: Pairwise and Multiple Sequence Alignment of nucleotide sequences using BLASTn and Clustal Omega.
  • 5 Sequence alignment: Pairwise and Multiple Sequence Alignment of protein sequences using BLASTp, Clustal Omega and PDB tool.
  • 6 Chemical Sketch Tool- Search for matching molecules in the PDB Chemical Component Dictionary.
  • 7 Visualization PDB Molecules using PyMOL and Swiss-PDB Viewer.
  • 8 Classification of Proteins using CATH and SCOP.
  • 9 Detect restriction enzyme sites on a sequence map and perform a custom digest with selected enzymes using NEBcutter.
  • 10 Predict domains, motifs, and functions of proteins using InterPro.
  • 11 PCR Primer design using NCBI Primer BLAST.
  • 12 Verification of sequence for a target gene and primers for amplification of the gene. (based on published article).

Reference Book

  • 1 Mount, David W.. Bioinformatics: Sequence and Genome Analysis. Thailand, Cold Spring Harbor Laboratory Press, 2004.
  • 2 Bioinformatics - Volume I: Data, Sequence Analysis, and Evolution. Second Edition Jonathan M. Keith, Monash University, Humana Press
  • 3 Bioinformatics for DNA Sequence Analysis, David Posada, University of Vigo, Spain, Humana Press 11 Web Links
  • 1 https://www.ncbi.nlm.nih.gov/
  • 2 https://www.ebi.ac.uk/jdispatcher/
  • 3 https://www.uniprot.org/
  • 4 https://www.ebi.ac.uk/interpro/
  • 5 https://www.rcsb.org/alignment
  • 6 https://www.pdbus.org/docs/search-and-browse/advanced-search/chemical- sketch-tool
  • 7 https://www.ebi.ac.uk/jdispatcher/msa/clustalo
  • 8 https://www.ncbi.nlm.nih.gov/tools/primer-blast/
  • 9 https://nc3.neb.com/NEBcutter/

Reproduced from the University of Mumbai syllabus for B.Sc. (Biotechnology) under NEP 2020, in force from the academic year 2025-26. Wording is as printed in that syllabus. Module numbering is as printed there too.

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