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Academic Council Item No. UNIVERSITY OF MUMBAI Revised Syllabus for T.Y.B.Sc. Programme- B.Sc. Course‐ Biotechnology (USBT) (Third Year – Sem. V & VI) (Credit Based Semester and Grading System with effect from the academic year 2016-2017)
TYBSc BT 16 17 REV SYLL 1 Syllabus Mumbai University by munotes
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T.Y.B.Sc. BIOTECHNOLOGY SEMESTER V AND VI REVISED SYLLABUS (CBSGS) 2016-2017 SEMESTER V COURSE TITLE UNIT TOPICS CREDITS L/WEEK I Cytoskeleton 1 USBT501 Cell Biology and II Cell membrane 1 Medical III Medical 2 Biotechnology Biotechnology- 1 Causative organisms IV Virology 1 I Immunology 1 USBT502 Biochemistry, II Endocrinology 1 Immunology and III Carbohydrate 2 1 Instrumentation metabolism IV Instrumentation 1 I Genetic mapping 1 USBT503 II Operon concept and Genetics and transposons 1 Molecular Biology 2 III Vectors and enzymes in Molecular Biology 1 IV Recombinant DNA libraries 1 I Types of fermentors USBT504 and process control 2 1 II Screening, Industrial inoculum and strain 1 Biotechnology development III Fermentations 1 IV Downstream processing 1 USBTP05 & 06 Practical Based on theory 2 9
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T.Y.B.Sc. BIOTECHNOLOGY SEMESTER V AND VI REVISED SYLLABUS (CBSGS) 2016-2017 SEMESTER VI COURSE TITLE UNIT TOPICS CREDITS L/WEEK I Cell signaling and USBT601 signal transduction 1 Cell Biology, Medical II Cell cycle, apoptosis Biotechnology and and cancer 2 1 Biostatistics III Chemotherapeutic agents 1 IV Biostatistics I Immunotechnology 1 USBT602 Biochemistry, II Endocrinology 1 Immunology and 2 III Lipid Metabolism 1 Instrumentation IV Instrumentation 1 I rDNA technology 1 USBT603 Molecular Biology 2 II Transgenic plants 1 and Bioinformatics III Transgenic animals 1 IV Bioinformatics 1 I Dairy technology 1 USBT604 II Environmental Industrial Biotechnology 1 Biotechnology 2 III Biofertilizers, Biopesticides, Biosurfactants and 1 biosensors IV IPR ,Quality Assurance, 1 Entrepreneurship USBTP07 & 08 Practical Based on theory 2 9 and Skill based project
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T.Y.B.Sc. BIOTECHNOLOGY SEMESTER V AND VI REVISED SYLLABUS (CBSGS) 2016-2017 SEMESTER V COURSE CODE TITLE CREDITS USBT501 Cell Biology and Medical Biotechnology 2 credits Overview of Cytoskeleton Unit I Microtubules: Structure, Composition, MAPs, Functions- Role in mitosis, Structural support and Cytoskeleton Intracellular motility. Motor proteins, MTOCs, Dynamic properties of microtubules, Microtubules in cilia and flagella. Drugs targeting microtubules Microfilaments: 15 lectures Structure, composition, Assembly and Disassembly Motor protein-myosin Muscle contractility- sliding filament model. Actin binding proteins: Examples of non-muscle motility Intermediate filaments: Structure and composition, Assembly and Disassembly Types and functions. Introduction and overview of functions: Unit II Fluid Mosaic Model, Chemical composition; Membrane Lipids- Phosphoglycerides, Sphingolipids, Cholesterol, Membrane Carbohydrates- Sugars, Oligosaccharides. Cell membrane Membrane Proteins- Integral, Peripheral, Lipid anchored. Membrane lipids and fluidity- Importance, Maintenance & Asymmetry. Dynamic nature- Flip flop and lateral movement. Cell permeability-Principles of membrane transport: Transporters and Channels. 15 lectures Active transport, Passive transport. Types of transporters, Types of ATP driven pumps - Na+ K+ pump. Cell junctions, cell adhesion and extracellular material Microvilli, Tight junctions, Gap junctions, Cell coat and cell recognition, Cellular interactions. ECM of animal connective tissue: Hyaluronic acid, GAGs, Proteoglycans.
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T.Y.B.Sc. BIOTECHNOLOGY SEMESTER V AND VI REVISED SYLLABUS (CBSGS) 2016-2017 Skin Infections- Staphylococcus: Unit III Characteristics Pathogenesis and immunity Toxins, Enzymes, Clinical Disease, Lab diagnosis, Medical Biotechnology- Treatment, Prevention and Control. Causative organisms Dermatophytes: Superficial and Cutaneous mycoses Respiratory Tract Infections- M. tuberculosis: Characteristics Transmission, Course of infection, Lab diagnosis, Management of TB, Prevention and Control, Immuno and Chemoprophylaxis, DOTS and MDR. Urinary Tract Infections- E.coli : Characteristics, Virulence, Clinical disease, Traveller’s diarrhoea and E.coli infections. 15 lectures GI Tract Infections- Salmonella: Characteristics, Virulence- Pathogenesis &Immunity, Clinical Disease, Carriers Lab diagnosis, Phage typing Prophylaxis and Treatment Sexually Transmitted Diseases- HIV: Characteristics Pathogenesis Transmission &Population at risk, Opportunistic infections, Lab diagnosis, Treatment, Prevention and Control, Vaccine development Parasitic Blood infection- Malarial parasites: Life Cycle, Diagnosis and Treatment Introduction to viruses-Position in biological spectrum Unit IV Virus properties, General structure of viruses Baltimore Classification and Taxonomy(ICTV), Cultivation of viruses, Virology Virulent phages and Lytic cycle - T even phages, One step growth experiment Temperate phages and lysogeny - lambda phage, 15 lectures Reproduction of ds DNA phages, animal viruses and plant virus Virus purification and assays Cytocidal infections and cell damage Viruses and cancer Viroids and Prions
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T.Y.B.Sc. BIOTECHNOLOGY SEMESTER V AND VI REVISED SYLLABUS (CBSGS) 2016-2017 COURSE CODE TITLE CREDITS USBT502 Biochemistry, Immunology and Instrumentation 2 credits Structure and Function of the Immune system: Unit I Cells and organs of immune system Membrane receptors for antigens: MHC–Class I and Class II molecules. Immunology Antigen Presentation by Endocytic and Exocytic Pathway Immune response Theories, B Cell Receptor, T Cell Receptor and Accessory Molecules CD4, CD8. 15 lectures Complement system : Classical Pathway and Alternate Pathway. Mechanism of action of group II hormones Unit II Storage, Release, transport, functions and disorders of – Hypothalamic hormones Endocrinology Anterior Pituitary hormones – GH & stimulating hormones (hCG, LH, FSH, TSH) 15 lectures Posterior pituitary hormones – ADH and oxytocin Adrenal medulla hormones – epihephrine and nor- epinephrine Pancreatic hormones – Insulin and Glucagon Biochemical pathway for Synthesis and regulation of Unit III carbohydrates in Bacteria –Peptidoglycan Carbohydrate metabolism Plants - Calvin cycle, C4, CAM, starch and sucrose 15 lectures Animals – Glycogen synthesis and breakdown Gluconeogenesis Chromatography: Unit IV Principle , working and application of Affinity, Ion-exchange, Gel permeation, Instrumentation HPLC and GC. 15 lectures Centrifugation: Types, principle, working and applications of Differential and Density Gradient - Isopycnic, Rate zonal, Gradient materials, preparation, sample application, recovery, choice of rotors.
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T.Y.B.Sc. BIOTECHNOLOGY SEMESTER V AND VI REVISED SYLLABUS (CBSGS) 2016-2017 COURSE CODE TITLE CREDITS USBT503 Genetics and Molecular Biology 2 Genetic mapping in bacteria and Bacteriophages: Unit I Gene mapping in bacteria by conjugation, transformation and transduction. 15 lectures Genetic mapping Mapping bacteriophage genes, Fine structure analysis of bacteriophage gene, Deletion mapping, and Defining genes by complementation tests. Regulation of gene expression in bacteria and Unit II bacteriophages: Operon concept. Operon concept and lac and trp operons- Gene organization and regulation. transposons Regulation of gene expression in phage λ. 15 lectures Transposons in prokaryotes and eukaryotes- IS elements, composite and non-composite transposons, plant transposons Transposable phage (phage Mu) Enzymes - Sources, types, mode of action and Unit III applications of Restriction endonucleases, DNA polymerases, Ligases, Kinases, Phosphatases, Terminal Vectors and enzymes in transferases, Reverse transcriptases and Nucleases, Molecular Biology Sequenase and Taq polymerase 15 lectures Vectors - Features and applications of pBR322, pUC19, cosmids, λ phage, M13 bacteriophage vector, Shuttle vector, Expression vector, Ti plasmid, Artificial chromosomes- YAC, BAC Construction and screening of Genomic libraries, Unit IV cDNA libraries, Chromosome libraries. Probes-synthesis (Random priming, Nick translation) 15 lectures Recombinant DNA libraries Use of probes to identify genes and cDNA in libraries. Molecular analysis of cloned DNA- restriction mapping
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T.Y.B.Sc. BIOTECHNOLOGY SEMESTER V AND VI REVISED SYLLABUS (CBSGS) 2016-2017 COURSE CODE TITLE CREDITS USBT504 Industrial Biotechnology 2 Fermentors – Unit I Acetator & Cavitator, Tower fermentor, Airlift fermentor, Bubble column fermentor, Deep jet Types of fermentors and fermentor, Packed Bed, Bioreactors for animal cell Process control culture, Stirred tank fermentor, Packed glass bead reactor. 15 lectures Process Control-Temperature control, flow measurement, pressure, biomass, DO, pH, redox, inlet and exit gas analysis. Addition of inoculum and nutrients. Sampling port, feed port, sensor probes, foam control. Unit II Primary screening, secondary screening, inoculum and Screening, inoculum and strain development 15 lectures strain development. Scale up, scale down Wine and beer: Introduction, manufacturing/processing, Unit III spoilage 15 lectures Malo-lactic fermentation Industrial Fermentations Production of : Penicillin, Streptomycin, Vinegar, Citric acid Single cell protein (Mushroom) Introduction of DSP Unit IV Foam separation, Types of Precipitation, Downstream processing Filtration, Centrifugation, Chromatography in DSP 15 lectures Cell disruption- physical and chemical methods. Solvent recovery, Membrane processes, Drying, Crystallization and Whole broth processing.
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T.Y.B.Sc. BIOTECHNOLOGY SEMESTER V AND VI REVISED SYLLABUS (CBSGS) 2016-2017 PRACTICALS COURSE CODE TITLE CREDITS Determination of Total RBC count Determination of Total WBC count Differential staining of Blood Film Medical Biotechnology: 9 Identification of S.aureus-Isolation, Catalase, Coagulase test USBTP05 9 Identification of E.coli-Isolation, Sugar 1 fermentations, IMViC 9 Identification of Salmonella- Isolation, Sugar fermentations, TSI slant MIC and MLC of antibiotic Preparation of Solutions Glucose estimation from serum using GOD-POD method Estimation of starch (Willstater’s method) Isolation of plasmid bearing culture and extraction of plasmid DNA and demonstration of its presence by agarose gel electrophoresis Transformation and Screening for transformants by replica plate method Construction of restriction map USBTP06 Problems on mapping of bacterial genes 1 Bioassay of penicillin Isolation of antibiotic producing organism- crowded plate technique & Wilkins agar overlay technique Encapsulation of yeast and estimation of invertase activity Vinegar analysis
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T.Y.B.Sc. BIOTECHNOLOGY SEMESTER V AND VI REVISED SYLLABUS (CBSGS) 2016-2017 SEMESTER VI COURSE CODE TITLE CREDITS USBT601 Cell biology and Medical Biotechnology 2 credits General Principles of cell communication: Unit I Introduction, Extracellular signal molecules binding to receptors, Cell signalling & Signal Forms of intercellular signalling - Autocrine, Transduction Contact dependent, Paracrine, Synaptic and Endocrine. Role of gap junction in signalling Response to multiple extracellular signal 15 lectures molecules Morphogens, Lifetime of intracellular molecule, Role of Nitric oxide and nuclear receptors Binding reaction and role of Kd, Extracellular messengers and their receptors, GPCRs RTKs, Second messengers Role of Calcium- Introduction, Calcium binding proteins Cell cycle- Overview of cell cycle and Phases, Unit II Check points Apoptosis and Necrosis: Definition and Process Cell cycle, apoptosis and Apoptosis: Caspases and targets, Factors which cancer regulate apoptotic death in normal cells. Significance and role of apoptosis in various diseases. 15 lectures Cancer: Definition, Characteristics of normal cell and cancerous cell. Tumor- Benign and malignant, types of cancer, oncogenes and tumor suppressor genes, invasion metastasis, angiogenesis, preventive measures for cancer Cancer vaccines Discovery and Design of antimicrobial agents: Unit III Classification of Antibacterial agents Selective toxicity, MIC,MLC, Chemotherapeutic agents Inhibition of cell wall synthesis: Beta lactam antibiotics, Penicillins Cephalosporins, Glycopeptides Vancomycin, Polypeptides Bacitracin, 15 lectures
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Injury to Plasma membrane: Polymyxin Inhibition of protein synthesis: Aminoglycosides, Tetracyclines Chloramphenicol, Macrolides-Erythromycin Inhibition of Nucleic acid synthesis: Quinolones Rifampin Metronidazole, Antimetabolites Sulphonamides, Trimethoprim Drug Resistance: Mechanism, Origin and transmission of drug resistance Use and misuse of antimicrobial agents, Antifungal drugs, Antiviral drugs Concept of Biostatistics- Definition of Unit IV biostatistics, terms used and Scope in Biological sciences, Biostatistics Types of Data and Representation of data using frequency distribution diagram (Simple/Multiple/Subdivided bar diagram, Pie chart), Graphs (Histogram, polygon, curve) Normal probability curve Measures of central tendency: Mean – Arithmetic mean, assumed mean. Median- raw data and grouped data. 15 lectures Mode: - raw data and grouped data. Measures of dispersion: Concept of Variance, Standard deviation and Coefficient of variance Coefficient of correlation and Regression analysis: Steps in testing statistical hypothesis: Parametric tests:- Z test – Single mean and Two means, t-test – Single mean, Paired and unpaired Nonparametric test:- Chi-square test.
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T.Y.B.Sc. BIOTECHNOLOGY SEMESTER V AND VI REVISED SYLLABUS (CBSGS) 2016-2017 COURSE CODE TITLE CREDITS USBT602 Biochemistry, Immunology and 2 credits Instrumentation Antigen-antibody reaction Unit I General Features of Antigen antibody reactions Measurements of Antigen-antibody reactions Immunotechnology Types of Serological Reactions: Precipitation, Agglutination, Flocculation. Types and Application of Immunoassay techniques: RIA, ELISA, Elispot, Immuno 15 lectures precipitation, Immunoflourescence. FACS ImmunoBlotting Diagnostic Tests: Coomb’s test and Complement Fixation Test Definition and Classification of hormones based Unit II on chemical nature and mode of action Mechanism of action of group I hormones: Endocrinology Storage, release, transport, functions and disorders of – Thyroid hormones – T3 and T4 15 lectures Adrenal cortex hormones – Glucocorticoids and mineralocorticoids Calcitriol Hormones of Gonads – Androgen, estrogen, progesterone. Biochemical pathway for Synthesis and Unit III regulation of- Fatty acids (even and unsaturated), Lipid metabolism Triacylglycerol, Phospholipids (Phosphatidyl 15 lectures choline, Phosphatidyl ethanolamine and Phosphatidyl serine), Cholesterol and Ketone bodies Isotopes in Biology: Detection Techniques of Unit IV Radioactivity using GM counter, Scintillation counter, Applications of Tracer techniques in Instrumentation Biology 15 lectures Microscopy: Principle, working and applications of TEM,SEM & confocal Spectroscopy- Principle, working and applications of IR and Spectrofluorimetry
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T.Y.B.Sc. BIOTECHNOLOGY SEMESTER V AND VI REVISED SYLLABUS (CBSGS) 2016-2017 COURSE CODE TITLE CREDITS USBT603 Molecular Biology and Bioinformatics 2 Principle, method and applications of Southern Unit I Hybridization, DNA fingerprinting, RFLP, DNA sequencing(Sangers, Automated) rDNA technology PCR(qPCR /Real time PCR, RT PCR) 15 lectures Vaccines: Sub unit vaccines(HSV), Peptide vaccines(FMDV), Attenuated vaccines(cholera), vector vaccines(vaccinia), DNA vaccine Genetic engineering of plants- Methodology. Unit II Plant transformation with the Ti plasmid of A.tumefaciens, Ti plasmid derived vector system Transgenic plants Physical methods of transferring genes to plants- 15 lectures electroporation, microprojectile bombardment, liposome mediated, protoplast fusion. Golden rice, bt cotton and edible vaccines Transgenic mice- methodology-retroviral Unit III method, DNA microinjection, ES method, genetic manipulation with cre-loxP Transgenic animals recombination system. Cloning live stock by 15 lectures nuclear transfer. Green Fluorescent Protein, Transgenic fish Bioinformatics:- Definition, history, scope and Unit IV applications of bioinformatics. Role of computer, internet, world wide web, Bioinformatics NCBI. Database:- Types of databases DNA databases 15 lectures Protein databases Tools :- Web search tools, Data retrieval tools – Entrez databases Heuristic tools in Sequence similarity searching - FASTA and BLAST
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T.Y.B.Sc. BIOTECHNOLOGY SEMESTER V AND VI REVISED SYLLABUS (CBSGS) 2016-2017 COURSE CODE TITLE CREDITS USBT604 Industrial Biotechnology 2 Milk: Unit I Normal flora, changes in raw milk, enumeration. Factorsaffectingbacteriological quality. Dairy technology Preservation methods, Pasteurisation. Starter Cultures. 15 lectures Fermented products-Production process and spoilage- Cheese: Swiss and Cheddar, Butter, Yogurt and Buttermilk Industrial Waste- Characteristics, Unit II Nature, Treatment Types-Dairy, Distillery and Pharmaceutical Environmental industry Biotechnology Monitoring criteria- pH, temp, TSS, TDS, TS, 15 lectures BOD, COD and heavy metals Energy sources renewable, non-renewable. Biofuels and biogas Bioremediation- plants and microbes Biopesticides and Biofertilizers- Unit III Production, application. advantages and limitations Biofertilizers, Biopesticides, Biosensors-principle, working and application 15 lectures and biosensors IPR-introduction, Patents, trade secrets Unit IV copyrights. Plant variety protection. Patenting genes and DNA sequences, genetic resources IPR ,Quality Assurance, Management of IPR 15 lectures Entrepreneurship Patenting related to GMO QA, QC and GMP Entrepreneurship
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T.Y.B.Sc. BIOTECHNOLOGY SEMESTER V AND VI REVISED SYLLABUS (CBSGS) 2016-2017 PRACTICALS COURSE CODE TITLE CREDITS Antibiotic sensitivity test using agar cup method, Antibiotic sensitivity test using paper disc method Antibiotic sensitivity test using ditch method. To determine synergistic action of two drugs. Problems based on : - USBTP07 Standard deviation , Coefficient of correlation, Regression, 1 Z-test , t-test chi-square test Cholesterol estimation in Serum Determination of Antigen identity by Ouchterlony and Mancini methods Sterility checking of injectible. Extraction of DNA from yeast Isolation of Normal flora from raw milk and curd Determination of milk protein USBTP08 Determination of BOD Determination of COD Enrichment and Isolation of Azotobacter and biopolymer production 1 Analysis of milk Methylene blue reduction test (MBRT) Resazurin reduction test (RRT) Phosphatase test for milk Skill based Project
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T.Y.B.Sc. BIOTECHNOLOGY SEMESTER V AND VI REVISED SYLLABUS (CBSGS) 2016-2017 REFERENCES Title Author Publisher Cell and Molecular Biology: Gerald Karp 5th edition Wiley International Concepts and Experiments, Student Version Molecular Biology of the Cell Alberts,B., Bray,D, Lewis,J, Garland Publishing, Inc Raff,M, Roberts,K, and Watson, J 3rd edition Biostatistics P.N.Arora and P.K. Malhan Himalaya Publishing House Methods in Biostatistics Mahajan ,B.K Jaypee brothers Textbook of Microbiology Pelczar, Kreig and Chan 5th edition Tata Mc Graw Hill Mims’ Medical Microbiology, Goering, R.V, Dockell, H.M, Elsevier Publications Zuckerman, M, Roitt, I.M. 5th edition Medical Microbiology Jawetz,E.,Brooks,G.E, Prentice Hall International Melnick,J.L., Butel,J.S, Adelberg, E. A 18th edition Foundations In Microbiology Talaro and Talaro Third edition W.C Brown Publishers Medical Microbiology Ananthnarayan 8th edition Orient Longman Microbiology Prescott Harley and Klein 5th edition Mc Graw Hill Principles of Biochemistry Lehninger Nelson and Cox 4th WH Freeman & Co edition Biochemistry Voet & Voet 3rd edition John Wiley & sons Introduction to Endocrinology Chandra S. Negi PHI learning pvt ltd Biochemistry and Molecular Buchanan, Gruissem and Jones IK International Biology of Plants Principles of Biochemistry Lehninger 2nd Ed Kalyani publications
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T.Y.B.Sc. BIOTECHNOLOGY SEMESTER V AND VI REVISED SYLLABUS (CBSGS) 2016-2017 Textbook of Medical Physiology Guyton, A.C and Hall 11th edition J.E Saunders Harper’s Illustrated Biochemistry Murray, R.K, Granner, M.D, Mayes, Mc Graw Hill P.A and Rondwell, V.W 27thEdition Biochemistry Satyanarayana 2nd edition Books and Allied pvt Ltd Kuby Immunology Goldsby, R.A, Kindt, T.J and W.H Freeman and Osborne, B.A, 5th Edition Company Biochemistry and Molecular Keith Wilson and John Walker 6th Cambridge University Biology edition Press Bioinstrumentation L. Veerakumari MJP Publishers Biophysical chemistry Upadhyay Upadhyay and Nath Himalaya Publishing House Practical Biochemistry, Principles Wilson and walker, 5th Edition Cambridge University and techniques Press Essential i genetics Peter Russell Pearson Education Molecular Biotechnology – Glick, B.R, Pasternak, J.J Patten, ASM press Principles and Applications of C.L 3rd edition Recombinant DNA Biotechnology expanding horizons BD Singh Kalyani Publishers Biotechnology-Fundamentals and S.S.Purohit 3rd edition Student edition Applications Basic Genetics Harlt,D Friedfelder,D and Snyder,L Jones and Bartlett Publishers Bioinformatics- methods and S.C.Rastogi, N. Mendiratta, PHL learning Pvt. Ltd. applications Genomics, Proteomics P.Rastogi 3rd edition and Drug discovery, Applied Dairy Microbiology Elmer H Marth and James L Steele Mercel Dekker Inc New 2nd edition York Microbial Technology Peppler,H.J and Perlman,D 2nd Academic Press edition Microbiology in Health and Frobisher WB Saunders and Disease Company Industrial Microbiology A.H.Patel 1st edition Macmillan India Environmental Biotechnology S.D. Jogdand Himalaya Publishing
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T.Y.B.Sc. BIOTECHNOLOGY SEMESTER V AND VI REVISED SYLLABUS (CBSGS) 2016-2017 ( Industrial pollution management) House Pharmaceutical Microbiology Hugo, W.B, Russell, A.D 6th edition Oxford Black Scientific Publishers Environmental Biotechnology Allan Scragg Oxford University press Environmental Biotechnology Indushekar Thakur IK International (Basic concepts and applications)
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T.Y.B.Sc. BIOTECHNOLOGY SEMESTER V AND VI REVISED SYLLABUS (CBSGS) 2016-2017 SUGGESTED READING Semester V Topic Cytoskeleton http://www.tifr.res.in/~roop/Publications_files/CurrBioMolMotor- Review.pdf Cell membrane http://assets.cambridge.org/97805217/04632/excerpt/9780521704632_exc erpt.pdf Medical http://www.ajtmh.org/content/77/6_Suppl/181.full.pdf+html Biotechnology- Causative organisms Virology http://www.cdc.gov/prions/pdfs/tse-in-humans.pdf Role of Factor H: A Complement Regulator in Health and Disease, and a Complement in Mediator of Cellular Interactions health and disease Biomolecules 2012, 2 46-75; doi:10.3390/biom2010046 Biomolecules ISSN 2218-273X Anne Kopp, Mario Hebecker, Eliška Svobodová, Mihály Józsi www.mdpi.com/journal/biomolecules/ The endocrine http://teachers.sduhsd.net/ahaas/Anatomy%20Physiology/Endocrine%20s system and its ystem/AandPendocrineclinicalapplicationswithquestions.pdf. disorders Disorders of REVIEW Dietary carbohydrate: relationship to cardiovascular disease and carbohydrate disorders of carbohydrate metabolism metabolism J Mann; European Journal of Clinical Nutrition (2007) 61 (Suppl 1), S100–S111 GC-MS Principle, Technique and its application in Food Science, REVIEW ARTICLE Syed Zameer Hussain and Khushnuma Maqbool INT J CURR SCI 2014, 13: E 116-126 ISSN 2250-1770 Reterotransposons www.sciencedirect.com/science/article/pii
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T.Y.B.Sc. BIOTECHNOLOGY SEMESTER V AND VI REVISED SYLLABUS (CBSGS) 2016-2017 Probes Probe design, production and applications pdf by M.A de Muro Fermentation. http://www.massey.ac.nz/~ychisti/FermentInd.PDF. Scale up http://fermentationtechnology.blogspot.com/2008/01/scale-down-in- fermentation-technology.html. Industrial http://www.gitam.edu/eresource/environmental/em_maruthi/industrial.htm fermentation DSP http://microbiology.ukzn.ac.za/Libraries/MICR304/DOWN_STREAM_P ROCESSING.sflb.ashx. Semester VI Topic Cell signaling and http://web.mit.edu/9.013/www/lectures/03- Signal 04_MS_Neuronal_Receptors.pdf transduction Cell cycle, http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3384434/pdf/aging-04- apoptosis and 330.pdf cancer http://www.radio.cuci.udg.mx/bch/ES/papers/CellCycleCheck_Encyclope dLifeSci_2002.pdf https://www.roswellpark.edu/sites/default/files/therapeutic_vaccines_for_ cancer_review_2014.111_april_7.pdf Chemotherapeutic http://icmr.nic.in/ijmr/2004/1009.pdf agents Biostatistics http://www.datastep.com/SPSSTutorial_1.pdf Immunodiagnostic Immunodiagnostics: Major Advances and Future Insights s Sandeep Kumar VashistVashist, Biochip Tissue Chip 2013, 3:2 http://dx.doi.org/10.4172/2153-0777.1000105 Role of hormone The Endocrine System and the Heart: A Review in heart disease Soo S. Rhee, Elizabeth N. Pearce Rev Esp Cardiol. 2011;64:220-31 - Vol. 64 Num.03 DOI: 10.1016/j.rec.2010.10.016
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T.Y.B.Sc. BIOTECHNOLOGY SEMESTER V AND VI REVISED SYLLABUS (CBSGS) 2016-2017 Lipoproteins LIPOPROTEINS – ROLE IN HEALTH AND DISEASES Edited by Saša Frank and Gerhard Kostner ISBN 978-953-51-0773-6 library.umac.mo/ebooks/b28050137.pdf Application of Application of Radioisotopes in Biochemical Analyses: Metal Binding Radioisotopes Proteins and Metal Transporters Miki Kawachi, Nahoko Nagasaki-Takeuchi, Mariko Kato and Masayoshi Maeshima http://www.intechopen.com/download/pdf/23692 Next generation www.illumina.com/technology/nextgenerationsequencinghtml sequencing technology Recombinant Vaccine development using recombinant DNA technology-CAST vaccines www.cast.science.org Green fluorescent The Green fluorescent protein Roger.Y.Tsein protein www.chem.ualberta.ca> resources>gfp Transgenic and www.lab.anhb.uwa.edu.au>tutorials knock out animals Starter cultures https://www.dairyscience.info/index.php/cheese-starters/49-cheese- starters.html Bioremediation http://www.moef.nic.in/downloads/public- information/BioremediationBook.pdf Biosurfactants http://www.dli.gov.in/rawdataupload/upload/insa/INSA_1/2000c4de_31.p df Filing a patent http://www.ipindia.nic.in/ipr/patent/manual/HTML%20AND%20PDF/Ma nual%20of%20Patent%20Office%20Practice%20and%20Procedure%20- %20html/Chapter%203.htm
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T.Y.B.Sc. BIOTECHNOLOGY SEMESTER V AND VI REVISED SYLLABUS (CBSGS) 2016-2017 List of You tube videos Semester V Topic Cytoskeleton https://www.youtube.com/watch?v=5rqbmLiSkpk Cell membrane https://www.youtube.com/watch?v=rR7NOSRyzhM Medical https://www.youtube.com/watch?v=odRyv7V8LAE Biotechnology- Causative organisms Virology https://www.youtube.com/watch?v=wLoslN6d3Ec Immunology Antigen presentation and processing https://www.youtube.com/watch?v=LwLYGTS_3EI Mode of action of group two hormones Endocrinology https://www.youtube.com/watch?v=Nt2r5R0ZO5U Carbohydrate Photosynthesis Light reaction, Calvin cycle, Electron Transport metabolism https://www.youtube.com/watch?v=wJDlxp17rY4 Instrumentation Separation on WBC using Ficoll density gradient centrifugation. https://www.youtube.com/watch?v=6lsldFFMEhE https://youtu.be/B7LfT7BIYSQ. Types of fermentors And process control Screening https://youtu.be/LYLjlSU7kOg. inoculums and strain development Fermentations https://youtu.be/zglgFHAPg7E DSP https://youtu.be/VKpthcW1llU https://youtu.be/N7vxq948l-U
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T.Y.B.Sc. BIOTECHNOLOGY SEMESTER V AND VI REVISED SYLLABUS (CBSGS) 2016-2017 Semester VI Topic Cell signaling https://youtu.be/qOVkedxDqQo and Signal transduction https://youtu.be/ZBSo_GFN3qI Cell cycle, https://youtu.be/brXqJIEx1EU apoptosis and cancer https://youtu.be/g1kEDSx23jU https://youtu.be/paw2SUY22_s https://youtu.be/67NhsSGsxC8 Chemotherapeut https://www.youtube.com/watch?v=cC9kyoAo1ac&list=PLMO1589WRspy ic agents kVPiy6SgKi3OPKbe9b0El Immunotechnolo Complement FixationTest gy https://youtu.be/lKAxV0WOaQE?list=PLewW6YqYSNaaqbPQDlxCHvggJ aCuoheoF ELISA https://youtu.be/lNxZxJtvB94?list=PLewW6YqYSNaaqbPQDlxCHvggJaC uoheoF Endocrinology Mode of action of group one hormones https://www.youtube.com/watch?v=CaMKuXKZ70g Lipid Familial hypercholesterolemia metabolism https://www.youtube.com/watch?v=Yanklj65zjs Instrumentation Confocal microscopy https://www.youtube.com/watch?v=jUAvneBhDcQ Dairy Cheese making Technology https://youtu.be/7-s2Kql0CHI Environment Biofuels Biotechnology https://youtu.be/ZGmwtDffc74 Biosensors https://youtu.be/8zbcib44XCc https://youtu.be/mliAE51s618 IPR Geographical indications - https://youtu.be/0darIuNMxk8
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T.Y.B.Sc. BIOTECHNOLOGY SEMESTER V AND VI REVISED SYLLABUS (CBSGS) 2016-2017 THEORY EXAMINATION PATTERN Internal Assessment- 25marks Sr. no Particulars Marks 1 Class test Objective type questions(10) 1/2 Concept based questions-Answer in one/two sentences (5) 1 Short notes-answer any two out of three 5 Total 20 2 Class participation 5 Semester end exam- 75 marks Sr. no Particulars 1 No of questions: 5 Questions 1-4 based on each unit Marks per question:15 No of subdivisions per question:2 a- Short answers-one/two sentences (with internal choice) b- Descriptive type (with internal choice) Question-5 medley of all four units-short notes(with internal choice) Total-75 Practical examination pattern Course USBTP05 USBTP06 Marks USBTP07 Marks USBTP0 Marks 8 Particulars Major I Major I 50 Major I 25 Major I 25 Major II Major II 50 Major II 25 Major II 25 Minor I Minor I 20 Minor I 10 Project 50 Minor II Minor II 20 Minor II 10 (Skill Journal Journal 20 Journal 10 based) Viva Viva 20 Viva 10 Identification and spots 20 Spots 10