SYBSc Biochemistry_1 Syllabus Mumbai University by munotes
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Page 1 of 19
AC – 7/07/2023
Item No. 6.2 (R)
UNIVERSITY OF MUMBAI
Revised Syllabus for the
S. Y. B.Sc. (Biochemistry)
(Sem. III & IV)
(Choice Based Credit System)
(With effect from the Academic year 2023-2024)
Page 4
Page 2 of 19 University of Mumbai
Syllabus for Approval
O: _____________ Title of Course
S.Y.B.Sc. (Biochemistry )
O: _____________ Eligibility
F. Y. B. Sc. Passed from this university (or
with ATKT in any two courses at the F. Y . B.
Sc. Level) or equivalent qualification from
other universities as may have been allowed
by the relevant ordinances of this university
R: ______________ Passing Marks
40%
No. of years/Semesters: -
Level: P.G. / U.G./ Diploma / Certificate
( Strike out which is not applicable)
Pattern: Yearly / Semester
( Strike out which is not applicable)
Status: Revised / New
( Strike out which is not applicable)
To be implemented from Academic Year : From Academic Year: 2023 -24
Prof. Shivram S. Garje,
Dean ,
Faculty of Science and Technology
Page 5
Page 3 of 19
S. Y. B.Sc.
Bio-Chemistry Syllabus
Choice Based Credit System
With effect from the Academic year 2023-2024
Summary
SEMESTER III
Course Code
and
Title UNIT TOPICS Credits L / Week
US BCH 301
Bio-organic
chemistry &
Biophysical
methods -I I Acids, bases, buffers and ionic equilibria 2 1
II Physicochemical principles 1
III Microscopy 1
US BCH 302
Fundamentals of
Genetics and
Physiology - I I Genetics I 2 1
II Blood and Body fluids 1
III Biological transport mechanisms 1
US BCH 303
Applied
Biochemistry I I Introduction to microbiology and cell
culture 2 1
II Fermentation and downstream
processing 1
III Industrial biotechnology 1
US BCH P3 Practicals based on both courses in theory 3 9
SEMESTER IV
Course Code
&
Title UNIT TOPICS Credits L / Week
US BCH 401
Bio-organic
chemistry &
Biophysical
methods - II I Enzymology 2 1
II Plant growth regulators and
endocrinology 1
III Approaches to biochemical
investigations 1
US BCH 402
Fundamentals of
Genetics and
Physiology - II I Genetics II 2 1
II Movement and locomotion 1
III Neurophysiology 1
US BCH 403
Applied
Biochemistry II I Trends in biotechnology 2 1
II Introduction to pharmacology 1
III Resource management 1
Page 6
Page 4 of 19 US BCH P4 Practical’s based on both courses in theory 3 9
Page 7
Page 5 of 19 S. Y. B. Sc.
Bio-Chemistry Syllabus
Choice Based Credit System
With effect from the Academic year 2023-2024
SEMESTER III
Course Code Title Credits
US BCH 301 Bio-organic chemistry & biophysical methods 2 Credits
(45 lectures)
Unit I: Acids, bases, buffers and ionic equilibria
15 Lectures 1.1 Definition – pH, pK, pKw, isoelectric pH, buffer, buffering
capacity
1.2 Derivations: Ionic product of water, Henders on–
Hasselbalch equation,
1.3 Relation between pI, pKa 1 and pKa 2 for a neutral,
acidic and basic amino acid
1.3.1 Ionization and titration curves of glycine, lysine and
aspartic acid; pKa, pHm, and pI values of these amino
acids
1.3.2 Sorensen’s reaction and formol titration of amino
acids
1.4 Physiological buffers:
Hb - HHb, carbonate bicarbonate, phosphate
and protein -proteinate
1.5 Numerical questions on above concepts.
Unit II: Physico -chemical principles
15 Lectures 2.1 Diffusion and osmosis
2.1.1 Ways of expressing solute concentration - mole,
molal, normal, percent, activity & ionic strength.
2.1.2 Diffusion & diffusion coefficient and factors affecting
diffusion of solute in solution
2.1.3 Osmosis – Van’t Hoff’s law of osmotic pressure law &
mathematical expression (no derivation), mechanism
of osmosis, role of osmosis in physiology.
2.1.4 Renal dialysis: Principles and process
2.2 Colloids and viscosity
2.2.1 Colloidal state in relation to surface forces, surface area,
electrical charge, precipitation and
Page 8
Page 6 of 19 flocculation.
2.2.2 Surface tension and its measurement, factors
affecting surface tension (Example: Role of bile in
digestion )
2.2.3 Viscosity - definition, measurement; Donnan ’s
membrane equilibrium, relation between
Donnan ’s equilibrium and osmotic pressure.
Unit III: Microscopy
15 Lectures 3.1 History, Basic principles of microscopy, of light and
colour.
3.2 Dissection and compound light microscope:
Construction and parts of a microscope, function of
each part, levels of magnification, concept of
refractive index and role of oil in magnification
3.3 Specialized microscopy I
3.3.1 Differential interference contrast (DIC),
3.3.2 Phase contrast,
3.3.3 Dark Field
3.4 Specialized Microscopy II
3.4.1 Simple fluorescence microscopy
3.4.2 Confocal microscopy
3.4.3 Electron microscopy
Principle, applications and comparative study
Types - SEM and TEM
Course Code Title Credits
USBCH302 Fundamentals of Genetics and Physiology 2 Credits
(45 lectures)
Unit I: Genetics: I
15 lectures 1.1 History: Contributions of Mendel, Bateson, Hardy -
Weinberg, Garrod, Morgan, Griffith, Beadle and
Tatum, Avery, MacLeod, McCarty, Lederberg,
Tatum, Barbara Mc Clintock, Hershey & Chase,
Watson &Crick.
1.2 Mendelian genetics: Mendel’s experiments -
Monohybrid, Dihybrid crosses, Laws of inheritance
1.3 Dominan t and recessiv e traits, co-dominance,
incomplete (semi) dominance, lethal genes
Page 9
Page 7 of 19 1.4 Gene interaction -Epistasis and it’s types,
multiple alleles, maternal effects
1.5 Numerical questions on the above concepts
Unit II: Blood and Body Fluids
15 lectures 2.1 Fluid compartments of the body –ICF and ECF
2.2 Blood : Composition, characteristics and function; role
of plasma proteins, Starling ’s hypothesis; blood
clotting and factors involved in blood clotting
2.3 Bile: Composition, characteristics and function;
storage
2.4 Urine : Composition –normal and abnormal
constituents; formation of urine.
2.5 Lymph : Composition, Formation and Circulation
Unit III: Biological transport mechanisms
15 lectures 3.1 Transport in plants : Role of xylem and phloem
3.2 Transport in blood :
3.2.1 Transport of gases CO 2 and O2, Role of hemoglobin,
O2 dissociation curves, Bohr effect , Chloride shift
3.2.2 Transport of Metabolites: transport of lipids –
lipoproteins and their types, role of plasma protein,
albumin in transport of metabolites and drugs
3.2.3 Transport of Ions: Fe -Ferritin and transferrin and
calcium
3.3 Transport across cell membrane s
3.3.1 Channel proteins and Carrier proteins
3.3.2 Passive transport (simple and facilitated diffusion)
with suitable examples; concept of symport, antiport,
uniport, Endocytosis and Exocytosis – with one
example each
3.3.3 Active transport: primary –Na+& K+ pump,
secondary Glucose -amino acid transport, types of
glucose transporters (GLUT 1 to GLUT 4),
aquaporins, ion channel inhibitors like gramicidin
and valinomycin
Page 10
Page 8 of 19 Course Code Title Credits
USBCH 303 Applied Biochemistry I (45 lectures)
Unit I: Microbiology in Human Health and Diseases
15 lectures 1.1 Beneficial Microorganism : Lactobacillus, Normal flora of
human gut, Probiotics, Yeast, Nitrogen fixing bacteria
(Rhizobium and Azotobacter)
1.2 Harmful microorganisms :
1.2.1 Air borne - Mycobacterium tuberculae
(Tuberculosis), Corynebacterium diptheriae
(Diptheria), Candida sp., Haemophilus influenza e
(Influenza), morbillivirus (measles)
1.2.2 Water borne - Shigella sp. (Dysentery), Vibrio cholerae
(Cholera), Salmonella sp. (Enteric fever), Hepatitis virus
1.2.3 Food borne - Staphylococcus aureus, Clostridium
botulinum (Botulism)
1.2.4 Soil borne - Clostridium tetani
1.3 Virology - General structure of a typical virus,
classification of viruses based on genome (DNA, RNA);
symmetry (helical, icosahedral, complex), host (bacteria,
plant, animal, insect); Lytic and Lysogenic cycle
Unit II: Cell and Tissue Culture
15 lectures 2.1 Plant Tissue Culture :
2.1.1 History, Introduction or definition (explants, callus,
dedifferentiation, re -differentiation) concept of
totipotency
2.1.2 Culture techniques; Types of culture (Callus culture,
Organ culture, protoplast culture, cell culture)
2.1.3 Applications: secondary metabolites in plant culture,
Micropropagation
2.2 Animal Cell Culture :
2.2.1 History, Introduction to Primary cell culture, Cell
lines (Finite and continuous)
2.2.2 Culture techniques used for primary culture,
2.2.3 Stem cell culture, Animal Organ Culture, Whole embryo
culture
2.2.4 Applications: hybridoma (monoclonal antibody),
production of Vaccines
Page 11
Page 9 of 19 Unit III: Industrial Biochemistry
15 lectures 3.1 Basics of fermentation
3.1.1 Typical Fermenter, Types of Fermenters (CSTF, Bubble
cap, Airlift, Fluidized Bed reactor)
3.1.2 Industrial production of wine, penicillin
3.2 Immobilized Enzyme : Introduction, Methods of
immobilization (entrapment, adsorption, covalent
binding, microencapsulation, cross linking)
3.2.1 Stabilization of soluble enzyme (solvent and substrate
stabilization, enzyme stabilization by polymer. Salts and
chemical modification)
3.3.2 Applications
3.3 Biosensors : Features of Biosensors, classification based
on transducers, applications
3.4 Single Cell proteins and their applications
Page 12
Page 10 of 19 References
Semester III
1. Hall J. E., Guyton C., (2015); Textbook of Medical Physiology (13th edn). Elsevier .
2. Tortora G. J., Derrickson B. H., (2017) ; Principles of Anatomy and Physiology, (15th edn).
Wiley and Sons.
3. Nelson D. L. , Cox M. M. , (2017) , Lehni nger Principles of Biochemistry, (4th edn) W.
H. Freeman & Company .
4. Jain J. L., (2016), Fundamentals of Biochemistry, (7th edn), S. Chand & Company .
5. Deb A. C., (2001), Fundamentals of Biochemistry , (9th edn), New Central Book
Agency (P) Ltd.
6. Satyanarayan U., Chakrapani U., (2021) , Biochemistry (6th edn) , Elsevier.
7. Murry R. K. & others, (2022), Harper’s I l l u s t r a t e d Biochemistry, (21st edn) ,
Mac Graw Hill .
8. Russe ll P. J., (2016) , i Genetics - A molecular Approach ( 3rd edn), Pearson Education
India.
9. Lewin B., (2000), Gene VII (7th edn) , Oxford University Press .
10. Strickberger M. W., (1995), Genetics (3rd edn), Prentice Hall India .
11. Wilson and Walker (2018), Principles and Techniques of Biochemistry and Molecular
Biology (8th edn), Cambridge University Press.
12. Pelczar M. J. Jr., Chan E. C. S., Krieg N. R., (2023), Microbiology (5th edn), Tata McGraw
Hill.
13. Purohit S. S. (2007) , Biotechnology : Fundamental and Applications (3rd edn) Agrobios.
14. Jogdand S. N. (2007) , Advances in Biotechnology (6th edn), Himalaya Publishing House.
15. Frazier W. C. , Westhoff D. C. (2017), Food Microbiology (5th edn) , The McGraw hill
Companies
16. Stanier R. Y. (1999), General Microbiology (5th edn) , Palgrave Macmillan .
17. Freifelder D. (1990) , Microbial Genetics , Narosa Publishing House
18. Gardner E. J., Simmons M. J., Snustad P. D. (2006) , Principles of Genetics (8th edn) ,
Wiley .
19. Lodish H., Berk A., Matsudaira P., Kaiser C. A., Krieger M., Scott M. P. , Zipursky
L., Darnell J. (2003), Molecular Cell Biology (5th revised edn) , W. H. Freeman and
Company.
20. Verma S. K., Verma M. (2021), Plant physiology, Biochemistry and Biotechnology , Shree
Ram Publishers.
Page 13
Page 11 of 19
P3 (301) S. Y. B. Sc
BIOCHEMISTRY
Practical Syllabus P3
1) a] Preparation of beta Amylase/Urease/Invertase extract demonstration of the
activity Qualitatively.
b] Determination of the Achromic point of Salivary Amylase.
2) Preparation of Buffers and measurement of pH using pH papers and pH meter.
3) Acid – Base titration of a polyprotic acid [H2CO 3/H3PO 4/Glycine hydrochloride]
4) a] A study of some methods of cell rupture: effect of hypo, hyper and isotonic
solutions on cells of the onion peel /plant cell (Hydrilla/ Vallisneria/ Spirogyra)
b] Effect of organic solvents on cell rupture
5) Determination of the Viscosity of sucrose solution using Ostwald’s Viscometer.
6) Demonstration of Osmosis through a semi permeable membrane. Potato
Osmometer
P3 (302)
1) Mendel’s Laws:
a] Problems based on the laws
b] case studies based on the laws
2) A study of Human Karyotypes.
3) Isoelectric precipitation of Casein using an indicator.
4) Field visit /Assignment on vermiculture, organic farming, composting, biogas plant
followed by a detailed report of at least one [the visit is recommended with the
report, but in case it is not possible an assignment is mandatory] .
P3 (303)
1) Demonstration of the working of an autoclave and a hot air oven.
2) Optimization of curd – a demonstration.
3) Sterility testing of air by plate exposure technique. [in sterile zone, in lab] and of tap
water.
4) A study of various culture inoculation methods. (Streak plate, pour plate and spread
plate methods).
5) Cell count in a culture medium using optical density
6) Determination of the zone of inhibition of microorganisms using the agar well
method and disc method.
Page 14
Page 12 of 19 7) Flow sheet diagrams of industrial preparation of: a vitamin, an antibiotic, a food
item, an enzyme and an alcohol.
Page 15
Page 13 of 19 Semester IV
Course Code Title Credits
USBCH 401 Bio-organic chemistry & biophysical methods 2 Credits
45 lectures
Unit I: Enzymology
15 lectures 1.1 Definition – Enzyme, coenzyme, cofactor, apoenzyme,
holoenzyme, prosthetic group, active site , enzyme
specificity, Turnover number, specific activity, Katal, IU.
1.2 IUB / EC classification up to one digit. Enzyme specificity
: Fischer’s lock & key and Koshland ’s induced fit theories
1.3 Activation energy, mechanism of enzyme action (concept of
active site, single and bi - substrate reaction), factors
affecting enzyme activity – substrate concentration, pH,
temperature
1.4 Enzyme kinetics – Derivation of Michaelis - Menten
equation and Lineweaver Burk plot for mono -substrate
reactions and numerical problems based on them.
1.5 Enzyme inhibition – Reversible and Irreversible; competitive
and noncompetitive , (one example of each)
Numerical problems on above.
Unit II: Plant growth regulators and endocrinology
15 lectures 2.1 Plant growth regulators - Structure and function of - auxins,
gibberellins, cytokinins, ethylene and abscisic acid.
2.2 Definition of hormones, hormone receptor, endocrine &
exocrine glands
2.3 Classification of hormones on the basis of:
2.3.1 Distance of target tissue - autocrine, paracrine, endocrine.
Hierarchal organization of the mammalian endocrine
system
2.3.2 Chemistry - One example for each sub class.
2.4 Chemistry & physiological role of :
Thyroxine, oxytocin & vasopressin
Glucocorticoids
FSH, LH, Estrogen, Progesterone (Reproductive cycle)
Mode of action of steroid hormones and epinephrine.
(amplification cascade Only till the level of protein kinase A)
G protein not to be covered .
Unit III: Approaches to Biochemical investigations
15 lectures
3.1 Whole animal and plant studies - the advantages and
Page 16
Page 14 of 19 disadvantages of any four model systems for biochemical
investigation
(e.g., Escherichia coli, Saccharomyces cerevisiae,
Dictyostelium discoideum , Caenorhabditis elegans ,
Drosophila melanogaster , Arabidopsis thaliana )
3.2 Organ & tissue studies
3.2.1 Isolated and cultured tissue and cell techniques: isolation,
culture and counting of cells.
3.3 Cell Fractionation
3.3.1 Cell rupture – solid shear, liquid shear, high pressure,
ultrasound, osmotic shock, chemical treatment (enzyme,
organic solvent), temperature.
3.3.2 Choice of suspension medium (isotonic & hypotonic
solution, PBS) and separation methods.
Page 17
Page 15 of 19 Course Code Title Credits
USBCH402 Fundamentals of Genetics and Physiology 2Credits
45 lectures
Unit I: Genetics: II
15 lectures 1.1 Genome organization
1.1.1 Prokaryotic Genome: Nucleoid structure
1.1.2 Eukaryotic chromosomes: Packaging of DNA (up to Solenoid
structure), DNA supercoiling, Topoisomerase, Chromatin
structure -Euchromatin, Heterochromatin, structure of
condensed chromatin, Centromere, kinetochore, telomere,
Comparison of chromosomal st ructure in prokaryotes and
Eukaryotes
1.2 Recombination in prokaryotes
1.2.1 Transformation: Transformation in S. pneumoniae
1.2.2 Transduction: General features with one example
1.2.3 Conjugation: Mechanism F+, F- and Hfr strain
Unit II: Movement and locomotion
15 lectures 2.1 Movement in plants
2.1.1 Movements of Locomotion Spontaneous: Ciliary, Amoeboid,
Cyclosis (Rotation, Circulation) Induced: Chemotaxis,
Phototaxis, Thermotaxis
2.1.2 Movements of Curvature: Mechanical: hygroscopic
movements
Vital:i)Spontaneous -movements of growth(nutation,
circumnutation , Hyponasty, epinasty); movements
of variation
ii)Induced –Tropic -hapto/geo/hydrotropism;
Nastic –seismonasty, Nyctinasty
2.2 Muscle contraction
2.2.1 Structural organization of a muscle fibre, myofibril
2.2.2 Contraction and Relaxation of Muscles; -mechanisms, Other
types of contractions –e.g. twitch, tetanus, Isotonic, Isometric
regulation of Muscle contraction
Unit III: Neurophysiology
15 lectures 3.1 Nervous System Classification : CNS, PNS; Components:
Neurons (3types) and Neuroglia (6types) –structure
and function, Axonal transport
Page 18
Page 16 of 19 3.2 Nerve impulse transmission :
Resting Membrane Potential, ion channels [voltage and
ligand gated], Action Potential (depolarization, polarization
and refraction period), propagation of action potential
(salutatory & continuous conduction)
3.3 Synaptic transmission :
Physiological anatomy of a synapse; – Electrical & Chemical
synapses, Excitatory & inhibitory postsynaptic potentials,
Agonists & Antagonists, inactivation of Neurotransmitter
3.4 Neurotransmitters :
Structure and function of acetylcholine, catecholamines,
GABA, glutamate, glycine
Page 19
Page 17 of 19 Course Code Title Credits
USBCH 403 Applied Biochemistry II 2 Credits
45 lectures
Unit I: Trends in Biotechnology:
15 lectures 1.1 Bioremediation:
1.1.1 Introduction to terms – Bioremediation,
Biotransformation, Xenobiotics, Recalcitrant
xenobiotics, Biomagnification, Factors affecting
bioremediation
1.1.2 Types of Bioremediations (In situ, Ex situ) ; Types of
reactions (Aerobic, anaerobic, sequential)
1.1.3 Applications of Biodegradation - hydrocarbons, (Oil
spills) Pesticides and herbicides, Heavy metals
(Uranium) contaminated soil and waste land, Ground
Water; Genetically Engineered Microbes in
bioremediation.
1.2 Biopesticides:
1.2.1 Introduction; Types of Biological Control (Classical,
inoculation, Inundation);
1.2.2 Examples of biopesticides from: Bacteria, Vir us, Fung i
and Protozoa .
1.3 Biofungicide: Examples and applications
1.4 Biofertilizers: Examples and applications
Unit II: Introduction to Pharmacology
15 lectures 2.1 Scope of pharmacology
2.2 Sources, Classification, Chemical & physical properties
of drug and Nomenclature of drugs
2.3 Dosage forms and routes of drug administration;
Factors affecting dosage and drug delivery
2.4 Pharmacokinetics: LD 50, ED 50 Half Life, Loading dose,
Maintenance dose (Explanation of terms only);
Therapeutic index
2.5 Novel Drug delivery system (NDDS):
2.5.1 Transdermal and oral modes
2.5.2 Liposomes and nanoparticles
Unit III: Resource management
15 lectures
3.1 Solid waste: Types of waste, treatment, recycling
Page 20
Page 18 of 19 3.2 Waste water : - Sewage
3.2.1 Composition of sewage, types of sewage, detection of
pathogenic organism in sewage; preliminary treatment,
primary treatment
3.2.2 Secondary treatment; tertiary treatment, disinfectant
3.2.3 Sludge treatment and disposal; waste water collection
vs sewage treatment in developing countries
3.3 Biomass and Bio energy production
3.3.1 Biofuel and Biomass: Fossil fuel; Energy rich crops
(sugar and starch; wood -rich; petroleum plants);
Animal energy; Sources of biofuel, its cultivation and
extraction process
3.3.2 Biogas: Production, Composition, Applications. Gobar
gas. [MSW and LFG, Renewable natural gas, NG vehicle]
Page 21
Page 19 of 19 References:
Semester IV
1. Hall J. E., Guyton C., (2015); Textbook of Medical Physiology (13th edn). Elsevier .
2. Tortora G. J., Derrickson B. H., (2017); Principles of Anatomy and Physiology, (15th edn).
Wiley and Sons.
3. Nelson D. L., Cox M. M., (2017), Lehninger Principles of Biochemistry, (4th edn) W.
H. Freeman & Company.
4. Jain J. L., (2016), Fundamentals of Biochemistry, (7th edn), S. Chand & Company.
5. Deb A. C., (2001), Fundamentals of Biochemistry, (9th edn), New Central Book
Agency (P) Ltd.
6. Satyanarayan U., Chakrapani U., (2021), Biochemistry (6th edn), Elsevier.
7. Murry R. K. & others, (2022), Harper’s I l l u s t r a t e d Biochemistry, (21st edn),
Mac Graw Hill.
8. Russell P. J., (2016), i Genetics - A molecular Approach (3rd edn), Pearson Education
India.
9. Lewin B., (2000), Gene VII (7th edn), Oxford University Press.
10. Strickberger M. W., (1995), Genetics (3rd edn), Prentice Hall India.
11. Wilson and Walker (2018), Principles and Techniques of Biochemistry and Molecular
Biology (8th edn), Cambridge University Press.
12. Pelczar M. J. Jr., Chan E. C. S., Krieg N. R., (2023), Microbiology (5th edn), Tata McGraw
Hill.
13. Purohit S. S. (2007), Biotechnology: Fundamental and Applications (3rd edn) Agrobios.
14. Jogdand S. N. (2007), Advances in Biotechnology (6th edn), Himalaya Publishing House.
15. Frazier W. C., Westhoff D. C. (2017), Food Microbiology (5th edn), The McGraw hill
Companies
16. Stanier R. Y. (1999), General Microbiology (5th edn), Palgrave Macmillan .
17. Freifelder D. (1990), Microbial Genetics, Narosa Publishing House
18. Gardner E. J., Simmons M. J., Snustad P. D. (2006), Principles of Genetics (8th edn),
Wiley.
19. Lodish H., Berk A., Matsudaira P., Kaiser C. A., Krieger M., Scott M. P., Zipursky
L., Darnell J. (2003), Molecular Cell Biology (5th revised edn), W. H. Freeman and
Company.
20. Verma S. K., Verma M. (2021), Plant physiology, Biochemistry and Biotechnology, Shree
Ram Publishers.
Page 22
Page 20 of 19
P4 (401) S.Y.B.Sc. BIOCHEMISTRY
Practical Syllabus P4
1) Parts and maintenance of a microscope.
2) Study of electron micrographs of cell organelles.
3) Permanent slides of Muscle tissue .
4) Recrystallization of Benzoic acid and determination of its yield.
5) Ammonium sulphate fractionation of protein and its estimation by a suitable
method.
6) Field visit/ assignment on any topic from the syllabus.
P4 (402)
1) Blood experiments:
i] Determination of total RBC count .
ii] Determination of total WBCcount .
2) Urine analysis:
i] Normal constituents - Urea, Uric acid
ii] Abnormal constituents – Glucose, Protein Ketone bodies, bile salts and bile
pigments.
iii] Titratable acidity [using neutral red or phenol red]
3) Bile :
i] Detection of Bilirubin [Iodine test / Gmelin’s Nitric acid test / Fouchet’s test]
ii] Detection of Bile salt [ Pettenkofer’s test. Hay’s sulphur test]
4) A demonstration of muscle twitch (using multimedia) .
5) Demonstration of plant movement. [A project to be handled in a group. Each group
to plan and execute the experiment in any way they choose. Results to be presented
to the class during practical sessions .]
P4 (403)
1) Isolation of DNA from Onion and confirmation by DPA test.
2) Determination of the Minimum Inhibitory Concentration of any one disinfectant.
3) Determination of the potability of water by conducting a coliform count. [ MPN]
4) Gram stain of sewa ge.
5) Determination of the Chemical Oxygen demand of an effluent / sewage.
6) Immobiliz ation of yeast/ amylase and determination of enzyme activity.
Page 23
Page 21 of 19
END SEMESTER THEORY ASSESSMENT (100 marks ) Duration of the examination shall be of
3 hours . The theory question paper pattern suggested is as follows:
1. There shall be four questions of 25 marks each.
2. On each unit there will be one question and fourth question will be based
on all the units.
3. All questions shall be compulsory with internal choice within the
questions.
4. The questions may be subdivided into sub questions as A, B, C etc.
THEORY:
Q1, Q2, Q3 to be based on Unit I, Unit II and Unit III of 25 marks each and Q4 to be
subdivided into A and B of 10 and 15 marks respectively based on Unit I, Unit II and Unit III.
Total 25 marks.
Options available: (For Q1, Q2, Q3) 25 marks each.
A. Objective: (No Internal options) 04 marks
Match the following / Answer in one sentence /Define / Give an example / Name the
following/ True or false.: Attempt 4 out of 6
B. Answer the following: Attempt 3 out of 6, each of 3 marks, 09 marks
C. Attempt 2 out of 4, each of 6 marks 12 marks
Q4 to be subdivided into 2 parts A and B based on Unit I, Unit II and Unit III Total 25 marks
A. Define/ Explain the term: Attempt 5 out of 7, each of 2 marks. 10 marks
B. Attempt 3 out of 6, each of 5 mark s with two questions from each unit. 15 marks
PRACTICAL:
Term End Exam: Practical 40 marks + Journal 05 marks + Viva 05 marks= Total 50
Marks .
Prof. Shivram S. Garje,
Dean ,
Faculty of Science and Technology