SE to BE Biotechnology Rev 2016 1 Syllabus Mumbai University by munotes
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University of Mumbai B. E. (Biotechnology) Rev 2016 Page 1
AC: 11 -05-2017
Item No. 4.188
UNIVERSITY OF MUMBAI
Revised syllabus (Rev - 2016) from Academic Year 2016 -17
Biotechnology
Second Year with Effect from AY 2017 -18
Third Year with Effect from AY 2018 -19
Final Year with Effect from AY 2019 -20
Under
FACULTY OF TECHNOLOGY
As per Choice Based Credit and Grading System
With effect from the AY 2016 –17
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University of Mumbai B. E. (Biotechnology) Rev 2016 Page 2
From Coordinator’s Desk
To meet the challenge of ensuring excellence in engineering education, the issue of quality needs to be
addressed, debated taken forward in a systematic manner. Accreditation is the principal means of
quality assurance in higher education. The major empha sis of accreditation process is to measure the
outcomes of the program that is being accredited. In line with this Faculty of Technology of University
of Mumbai has taken a lead in incorporating philosophy of outcome based education in the process of
curri culum development.
Faculty of Technology, University of Mumbai, in one of its meeting unanimously resolved that, each
Board of Studies shall prepare some Program Educational Objectives (PEO’s) give freedom to
affiliated Institutes to add few (PEO’s) cour se objectives course outcomes to be clearly defined for
each course, so that all faculty members in affiliated institutes understand the depth approach of course
to be taught, which will enhance learner’s learning process. It was also resolved that, maximu m senior
faculty from colleges experts from industry to be involved while revising the curriculum. I am happy
to state that, each Board of studies has adhered to the resolutions passed by Faculty of Technology,
developed curriculum accordingly. In additio n to outcome based education, Choice Based Credit and
Grading System is also introduced to ensure quality of engineering education.
Choice Based Credit and Grading S ystem enables a much -required shift in focus from teacher -centric
to learner -centric educa tion since the workload estimated is based on the investment of time in learning
not in teaching. It also focuses on continuous evaluation which will enhance the quality of education.
University of Mumbai has taken a lead in implementing the system through its affiliated Institutes
Faculty of Technology has devised a transparent credit assignment policy adopted ten points scale to
grade learner’s performance. Choice Based Credit and grading based system is implemented for
Second Year of B.E. in Biotechnol ogy Engineering from the academic year 201 7-2018. This system
will be carried forward for Third Year of B.E. in Biotechnology Engineering in the academic year
2018-2019 and for Fourth Year B.E. in the year 2019 -2020 respectively.
Dr. S. K. Ukar ande
Co-ordinator ,
Faculty of Technology,
Mem ber - Academic Council
University of Mumbai, Mumbai
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University of Mumbai B. E. (Biotechnology) Rev 2016 Page 3
Preamble to the Revision of Syllabus in Biotechnology Engineering
The onset of nineties brought about some paradigm shifts. One was in the sphere of
market economics. Suddenly the Indian manufacturing sector started jostling for a
place with international competition in the arena. The presence of International
products a t competitive rates and quality forced some small and medium scale units to
close their operations. The larger industry players realized the importance of R&D
and accordingly set up separate cells to optimize production and improve quality. The
second majo r impact was in the sphere of knowledge. With the advent of World Wide
Web in the early nineties and its subsequent growth, the latest research trends have
become accessible from drawing rooms across the globe. This acted as a positive
feedback mechanism i n increasing the pace of research in all fields including Bio -
technology. This was the motivation for an in depth analysis of what is actually
required for today’s technology. It is also important to take advantage of the freely
available software to enhan ce the quality and quantity of material that can be covered
in the class room.
With this scenario as the backdrop, the first meeting was conducted by Board of
Studies in Biotechnology at Thadomal Shahani College of Engineering Bandra on 3rd
February 2017. It was attended by the various heads of departments of Biotechnology
engineering as well as experts from industry. The academic scheme and exam scheme
of the program was discussed along with t he program objectives and outcomes. T he
core str ucture of the syllabus was formulated keeping in mind choice based credit
and grading system curriculum to be introduced in this revised syllabus for B.E.
(Biotechnology) for all semesters. A second meeting was held in Datta Meghe
College of Engineering Ai roli on 20th February 2017 and detailed syllabus of
Semesters III and IV was finalised. Subsequently another meeting was held in
Thadomal Shahani Engineering College Bandra on 11th April 2017 to finalise the
detail syllabus of subjects pertaining to semester V, VI, VII and VIII.
Dr. Kalpana S. Deshmukh,
Chairman, Board of Studies in Chemical Engineering (Adhoc),
University of Mumbai, Mumbai.
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University of Mumbai B. E. (Biotechnology) Rev 2016 Page 4
General Guidelines
Tutorials
The nu mber of tutorial batches can be decided based on facilities available in
the institution.
Tutorials can be creative assignments in the form of models, charts, projects,
etc.
Term Work
Term work will be an evaluation of the tutorial/practical done over the entire
semester.
It is suggested that each tutorial/practical be graded immediately and an
average be taken at the end.
A minimum of eight tutorials/ten practical will form the basis for final
evaluation.
The total 25 marks for term work (except project and seminar) will be
awarded as follows:
Tutorial / Practical Journal – 20 marks
Overall Attendance – 05
Further, while calculating marks for attendance, the following guidelines
shall be adhered to:
75 % to 80%. – 03 marks
81% to 90% - 04 marks
91% onwards – 05 marks
Theory Examination
In general all theory examinations will be of 3 hours duration.
Question paper will comprise of total six questions, each of 20 Marks.
Only f our questions need to be solved.
Question one will be compulsory and based on maximum part of the syllabus.
Note:
In question paper, weightage of each module will be propor tional to number
of respective lecture hours as mentioned in the syllabus as far a s possible.
Practical Examination:
Duration for practical examination would be the same as assigned to the
respective Lab per week.
A student becomes eligible for practical examination after completing a
minimum of eight experiments out of ten experime nts.
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University of Mumbai B. E. (Biotechnology) Rev 2016 Page 5
Project and Seminar Guidelines
Project Groups: Students can form groups with minimum 2 (Two) and not
more than 3 (Three)
The load for projects may be calculated proportional to the number of groups,
not exceeding two hours per week.
The load for projects may be calculated as:
Sem VII: ½ hr for teacher per group.
Sem VIII: 1 hr for teacher per group.
Each teacher should have ideally a maximum of three groups and only in
exceptional cases four groups can be allotted to the faculty.
Semin ar topics will be the consensus of the project guide and the students.
Each student will work on a unique topic.
The load for seminar will be calculated as one hour per week irrespective of
the number of students
Students should spend considerable time in applying all the concepts studied,
into the project. Hence, eight hours each were allotted in Project A, B and
three hours for Seminar to the students.
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University of Mumbai B. E. (Biotechnology) Rev 2016 Page 6
University of Mumbai
Program Structure for B.E. Biotechnology (Revised 2016 )
S.E. Semester III (w.e.f 2017 -2018)
Course
code Course Name Teaching Scheme
(Contact Hours) Credits Assigned
Theory Practical Tutorial Theory Practical Tutorial Total
BTC301 Applied
Mathematics -III 3 - 1 3 - 1 4
BTC302 Microbiology 4 - - 4 - - 4
BTC303 Cell Biology 3 - 1 3 - 1 4
BTC304 Biochemistry 4 - - 4 - - 4
BTC305 Unit Operations -I 3 - - 3 - - 3
BTC306 Process Calculations 3 - 1 3 - 1 4
BTL301 Microbiology Lab - 3 - - 1.5 - 1.5
BTL302 Biochemistry Lab - 3 - - 1.5 - 1.5
BTL303 Unit Operations -I
Lab - 2 - - 1 - 1
Total 20 8 3 20 4 3 27
Course
code Course Name Examination Scheme
Theory Term
Work Pract/
Oral Oral Total
Internal
Assessment End
Sem
Exam Exam
Duration
(in hrs)
Test 1 Test 2 Avg
BTC301 Applied Mathematics -III 20 20 20 80 3 25 - - 125
BTC302 Microbiology 20 20 20 80 3 - - 100
BTC303 Cell Biology 20 20 20 80 3 25 - - 125
BTC304 Biochemistry 20 20 20 80 3 - - 100
BTC305 Unit Operations -I 20 20 20 80 3 - - 100
BTC306 Process Calculations 20 20 20 80 3 25 - - 125
BTL301 Microbiology Lab 25 25
BTL302 Biochemistry Lab 25 25
BTL303 Unit Operations -I Lab 25 25
Total 120 480 - 75 50 25 750
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University of Mumbai B. E. (Biotechnology) Rev 2016 Page 7
University of Mumbai
Program Structure for B.E. Biotechnology (Revised 2016 )
S.E. Semester IV (w.e.f 2017 -2018)
Course
code Course Name Teaching Scheme
(Contact Hours) Credits Assigned
Theory Practical Tutorial Theory Practical Tutorial Total
BTC401 Applied Mathematics -IV 3 - 1 3 - 1 4
BTC402 Molecular Genetics 3 - 1 3 - 1 4
BTC403 Fermentation
Technology 4 - 4 - 4
BTC404 Analytical Methods in
Biotechnology 4 - - 4 - - 4
BTC405 Immunology and
Immunotechnology 3 - 1 3 - 1 4
BTC406 Unit Operations -II 3 - 3 - 3
BTL401 Fermentation
Technology Lab - 3 - 1.5 - 1.5
BTL402 Analytical Methods in
Biotechnology Lab - 3 - 1.5 - 1.5
BTL403 Unit Operations –II Lab - 2 - 1 - 1
Total 20 8 3 20 4 3 27
Course
code Course Name Examination Scheme
Theory Term
Work Pract/
Oral Oral Total
Internal
Assessment End
Sem
Exam Exam
Duration
(in hrs)
Test 1 Test 2 Avg
BTC401 Applied Mathematics -IV 20 20 20 80 3 25 - - 125
BTC402 Molecular Genetics 20 20 20 80 3 25 - - 125
BTC403 Fermentation Technology 20 20 20 80 3 - - 100
BTC404 Analytical Methods in
Biotechnology 20 20 20 80 3 - - - 100
BTC405 Immunology and
Immunotechnology 20 20 20 80 3 25 - - 125
BTC406 Unit Operations -II 20 20 20 80 3 - - - 100
BTL401 Fermentation Technology
Lab - - - 3 - 25 25
BTL402 Analytical Methods in
Biotechnology Lab - - - - 3 - 25 - 25
BTL403 Unit Operations –II Lab - - - - - - - 25 25
Total 120 480 - 75 50 25 750
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University of Mumbai B. E. (Biotechnology) Rev 2016 Page 8
University of Mumbai
Program Structure for B.E. Biotechnology (Revised 2016 )
T.E. Semester V (w.e.f 2018 -2019)
Course
code Course Name Teaching Scheme
(Contact Hours) Credits Assigned
Theory Practical Tutorial Theory Practical Tutorial Total
BTC501 Bioinformatics 4 - - 4 - - 4
BTC502 Genetic Engineering 4 - - 4 - - 4
BTC503 Thermodynamics and
Biochemical Engineering 3 - 1 3 - 1 4
BTC504 Bioreactor Analysis and
Technology 3 - 1 3 - 1 4
BTC505 Business Communication
and Ethics 2 - 2 - - 2 2
BTE501X Elective I 3 - 1 3 - 1 4
BTL501 Bioinformatics Lab - 2 - - 1 - 1
BTL502 Genetic Engineering Lab 3 1.5 1.5
BTL503 Lab I 3 1.5 1.5
Total 17 12 3 17 6 3 26
Course
code Course Name Examination Scheme
Theory Term
Work Pract/
Oral Oral Total
Internal
Assessment End
Sem
Exam Exam
Duration
(in hrs)
Test 1 Test 2 Avg
BTC501 Bioinformatics 20 20 20 80 3 - - 100
BTC502 Genetic Engineering 20 20 20 80 3 - 100
BTC503 Thermodynamics and
Biochemical Engineering 20 20 20 80 3 25 - - 125
BTC504 Bioreactor Analysis and
Technology 20 20 20 80 3 25 - - 125
BTC505 Business Communication
and Ethics - - - - - 50 - 50
BTE501X Department Elective I 20 20 20 80 3 25 - - 125
BTL501 Bioinformatics Lab 2 25 25
BTL502 Genetic Engineering Lab 3 25 25
BTL503 Lab I - - - - 3 25 - 25
Total 100 400 - 125 75 - 700
Department Elective I (Sem V)
Engineering Stream Advanced Science Stream Technology Stream
1. Biosensors and Diagnosis
(BTE5011) 1.Biophysics (BTE5012)
2.Biostatistics (BTE5013) 1. Pharmaceutical Technology
(BTE5014)
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University of Mumbai B. E. (Biotechnology) Rev 2016 Page 9
University of Mumbai
Program Structure for B.E. Biotechnology (Revised 2016 )
T.E. Semester VI (w.e.f 2018 -2019)
Course
code Course Name Teaching Scheme
(Contact Hours) Credits Assigned
Theory Practical Tutorial Theory Practical Tutorial Total
BTC601 Food Technology 3 - 1 3 - 1 4
BTC602 Cell & Tissue
Culture 4 - - 4 - - 4
BTC603 Enzyme
Engineering 4 - 4 - - 4
BTC604 IPR, Bioethics
and Bio safety 3 - 1 3 - 1 4
BTC605 Process Control &
Instrumentation 3 - 1 3 - 1 4
BTE60 2X Elective -II 3 - 1 3 - 1 4
BTL601 Lab-II - 3 - - 1.5 - 1.5
BTL602 Lab-III - 3 - - 1.5 - 1.5
Total 20 6 4 20 3 4 27
Course
code Course Name Examination Scheme
Theory Term
Work Pract/
Oral Oral Total
Internal
Assessment End
Sem
Exam Exam
Duration
(in hrs)
Test 1 Test 2 Avg
BTC601 Food Technology 20 20 20 80 3 25 - - 125
BTC602 Cell & Tissue
Culture 20 20 20 80 3 - - - 100
BTC603 Enzyme Engineering 20 20 20 80 3 - - - 100
BTC604 IPR, Bioethics and
Bio safety 20 20 20 80 3 25 - - 125
BTC605 Process Control &
Instrumentation 20 20 20 80 3 25 - - 125
BTE60 2X Elective -II 20 20 20 80 3 25 - - 125
BTL601 Lab-II - - - - 3 - 25 25
BTL602 Lab-III - - - - 3 - 25 - 25
l Total 120 480 - 100 50 -- 750
Department Elective II (Sem VI)
Engineering Stream Advanced Science Stream Technology Stream
1. Computational Fluid Dynamics
(BTE60 21) 1. Protein Engineering
(BTE602 2)
2. Cancer Biology (BTE602 3) 1. Green technology
(BTE60 24)
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University of Mumbai B. E. (Biotechnology) Rev 2016 Page 10
University of Mumbai
Program Structure for B.E. Biotechnology (Revised 2016 )
B.E. Semester VII (w.e.f 2019 -2020)
Course
code Course Name Teaching Scheme
(Contact Hours) Credits Assigned
Theory Practical Tutorial Theory Practical Tutorial Total
BTC701 Bioseperation & Downstream
Processing Technology -I 4 - 1 4 - 1 5
BTC702 Bioprocess Modelling and
Simulation 4 - 1 4 - 1 5
BTC703 Agriculture Biotechnology 3 - 1 3 - 1 4
BTE70 3X Department Elective III 3 - 1 3 - 1 4
ILO7 01X Institute Level optional
Subject I 3 - - 3 - - 3
BTP701 Project A - - 6 - - 3 3
BTL701 Lab - IV - 3 - - 1.5 - 1.5
BTL702 Lab - V - 3 - - 1.5 - 1.5
Total 17 6 10 17 3 7 27
Course
code Course Name Examination Scheme
Theory Term
Work Pract/
Oral Oral Total
Internal
Assessment End
Sem
Exam Exam
Duration
(in hrs)
Test 1 Test 2 Avg
BTC701 Bioseperation & Downstream
Processing Technology -I 20 20 20 80 3 25 - - 125
BTC702 Bioprocess Modelling and
Simulation 20 20 20 80 3 25 - - 125
BTC703 Agriculture Biotechnology 20 20 20 80 3 25 - - 125
BTE70 3X Department Elective III 20 20 20 80 3 25 - - 125
ILO7 01X Institute Level optional Subject I 20 20 20 80 3 - - - 100
BTP701 Project A - - - - - 100 50 150
BTL701 Lab - IV - - - - - - 25 - 25
BTL702 Lab - V - - - - - - 25 - 25
Total 100 400 - 200 50 50 800
Department Elective III (Sem VII)
Engineering Stream Advanced Science Stream Technology Stream
1. Stem Cell & Tissue Engineering
(BTE703 1) 1. Operation research in Biotechnology
(BTE703 2)
2. Project Management (BTE703 3) 1. Nanotechnology
(BTE703 4)
Institute Level Optional Subject I (Sem VII)
1. Product Lifecycle Management
(ILO7011) 4. Design of Experiments (ILO7014) 7. Disaster Management and
Mitigation Measures (ILO7017)
2. Reliability Engineering
(ILO7012) 5. Operation Research (ILO7015) 8. Energy Audit and
Management (ILO7018)
3. Management Information
System (ILO7013) 6. Cyber Security and Laws
(ILO7016) 9. Development Engineering
(ILO7019)
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University of Mumbai B. E. (Biotechnology) Rev 2016 Page 11
University of Mumbai
Program Structure for B.E. Biotechnology (Revised 2016 )
B.E. Semester VIII (w.e.f 2019 -2020)
Course
code Course Name Teaching Scheme
(Contact Hours) Credits Assigned
Theory Practical Tutorial Theory Practical Tutorial Total
BTC801 Environmental Biotechnology 4 - - 4 - - 4
BTC802 Bioseperation & Downstream
Processing technology -II 4 - - 4 - - 4
BTC803 Bioprocess Plant & Equipment
design 3 - 1 3 - 1 4
BTE80 4X Department Elective IV 3 - 1 3 - 1 4
ILO802 X Institute Level optional Subject II 3 - - 3 - - 3
BTP801 Project B - - 8 - - 6 6
BTL801 Lab - VI - 3 - - 1.5 - 1.5
BTL802 Lab - VII - 3 - - 1.5 - 1.5
Total 17 6 10 17 3 8 28
Course
code Course Name Examination Scheme
Theory Term
Work Pract/
Oral Oral Total
Internal
Assessment End
Sem
Exam Exam
Duration
(in hrs)
Test 1 Test 2 Avg
BTC801 Environmental Biotechnology 20 20 20 80 3 - - - 100
BTC802 Bioseperation& Downstream
Processing technology -II 20 20 20 80 3 - - - 100
BTC803 Bioprocess Plant & Equipment
design 20 20 20 80 3 25 - - 125
BTE804 X Department Elective IV 20 20 20 80 3 25 - 125
ILO802 X Institute Level optional Subject II 20 20 20 80 3 - - - 100
BTP801 Project B - - - - - 100 - 50 150
BTL801 Lab - VI - - - - 3 - 25 - 25
BTL802 Lab - VII - - - - 3 - 25 - 25
Total 100 400 - 150 50 50 750
Department Elective IV (Sem VIII)
Engineering Stream Advanced Science Stream Technology Stream
1. Non-conventional Sources of Energy
(BTE804 1) 1. Total Quality Management
(BTE804 2)
2. Entrepreneurship (BTE804 3) 1. Advanced Bioinformatics
(BTE804 4)
Institute Level Optional Subject II (Sem VIII)
1. Project Management (ILO8021) 4. Human Resource
Management (ILO8024) 7. IPR and Patenting
(ILO8027)
2. Finance Management (ILO8022) 5. Professional Ethics and CSR
(ILO8025) 8. Digital Business
Management (ILO8028)
3. Entrepreneurship Development and
Management (ILO8023) 6. Research Methodology
(ILO8026) 9. Environmental
Management (ILO8029)
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University of Mumbai B. E. (Biotechnology) Rev 2016 Page 12
University of Mumbai
Program Structure for B.E. Biotechnology (Revised 2016 )
S.E. Semester III (w.e.f 2017 -2018)
Course
code Course Name
Teaching Scheme
(Contact Hours) Credits Assigned
Theory Practical Tutorial Theory Practical Tutorial Total
BTC301 Applied Mathematics -
III 3 - 1 3 - 1 4
BTC302 Microbiology I 4 - - 4 - - 4
BTC303 Cell Biology 3 - 1 3 - 1 4
BTC304 Biochemistry 4 - - 4 - - 4
BTC305 Unit Operations -I 3 - - 3 - - 3
BTC306 Process Calculations 3 - 1 3 - 1 4
BTL301 Microbiology Lab - 3 - - 1.5 - 1.5
BTL302 Biochemistry Lab - 3 - - 1.5 - 1.5
BTL303 Unit Operations -I Lab - 2 - - 1 - 1
Total 20 8 3 20 4 3 27
Course
code Course Name
Examination Scheme
Theory
Term
Work Pract/
Oral Oral Total Internal Assessment End
Sem
Exam Exam
Duration
(in hrs) Test 1 Test 2 Avg
BTC301 Applied Mathematics -III 20 20 20 80 3 25 - - 125
BTC302 Microbiology I 20 20 20 80 3 - - - 100
BTC303 Cell Biology 20 20 20 80 3 25 - - 125
BTC304 Biochemistry 20 20 20 80 3 - - - 100
BTC305 Unit Operations -I 20 20 20 80 3 -- - - 100
BTC306 Process Calculations 20 20 20 80 3 25 - - 125
BTL301 Microbiology Lab - - - - 3 -- 25 - 25
BTL302 Biochemistry Lab - - - - 3 -- 25 - 25
BTL303 Unit Operations -I Lab - - - - - -- - 25 25
Total 120 480 - 75 50 25 750
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University of Mumbai B. E. (Biotechnology) Rev 2016 Page 13
Course Code Name of Subject Credits
BTC301 Applied Mathematics III 04
Pre-requisites :
Basics of complex numbers: modulus, argument; equation of a circle, roots of
unity, Euler’s formula; hyperbolic functions; matrices: symmetric, orthogonal and
unitary matrices, rank, normal form, solutions of systems of linear equations;
basics of LPP: graphical method; calculus: partial derivatives, Hessian,
maxima/minima of functions of 1 and 2 real variables.
Course Objectives:
To introduce students to the basic methods of Laplace transfo rms.
Laplace transforms and inverse Laplace transforms of all the standard functions.
To enable students to solve initial value ODE problems using L -transforms.
To study eigen values and eigen spaces of matrices.
Orthogonal and congruent reduction of quadratic forms.
Complex analysis: C -R equations, Milne -Thomson method.
Bilinear transformations and cross -ratios.
Introduction to statistics.
Lagrange multiplier method for 2 and 3 variables with no more than two
constraints.
To introduce the basics of op timization using Kuhn -Tucker conditions.
Course outcomes:
The student will be able to solve initial value ODE problems.
The student will have a good understanding of real and complex analysis.
The student will have a thorough grounding in matrix algebra.
The student will be ready for any further courses on optimization.
Module Contents Contact
Hours
01 The Laplace transform: Definition and properties (without
proofs); all standard transform methods for elementary functions
including hyperbolic functions; Heaviside unit step function,
Dirac delta function; the error function; evaluation of integrals
using Laplace transforms; inverse Laplace transforms using
partial fractions and H(t -a); convolution (no proof). 07
02 Matrices: Eigen values and eigens paces of 2x2 and 3x3 matrices;
existence of a basis and finding the dimension of the eigen space
(no proofs); non - diagonalisable matrices; minimal polynomial;
Cayley - Hamilton theorem (no proof); quadratic forms;
orthogonal and congruent reduction of a quadra tic form in 2 or 3
variables; rank, index, signature; definite and indefinite forms. 07
03 Complex analysis: Cauchy -Riemann equations (only in Cartesian
co- ordinates) for an analytic function (no proof); harmonic
function; Laplace’s equation; harmonic conjugates and orthogonal
trajectories (Cartesian co - ordinates); to find f(z) when u+v or u - 07
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University of Mumbai B. E. (Biotechnology) Rev 2016 Page 14
v are given; Milne -Thomson method; cross -ratio (no proofs);
conformal mappings; images of straight lines and circles.
04 Complex Integrati on Cauchy’s integral formula; poles and
residues; Cauchy’s residue theorem; applications to evaluate real
integrals of trigonometric functions; integrals in the upper half
plane; the argument principle. 06
05 Statistics: (No theory questions expected in this module) Mean,
median, variance, standard deviation; binomial, Poisson and
normal distributions; correlation and regression between 2
variables. 05
06 Optimization (No theory) . Non-linear programming: Lagrange
multiplier method for 2 or 3 variables with at most 2 constraints;
conditions on the Hessian matrix (no proof); Kuhn -Tucker
conditions with at most 2 constraints. 07
Term work
Term work shall consist of minimum eight tutorials from entire syllab us which
are to be given at regular intervals Batch wise.
Tutorials: 20 marks
Attendance: 05 marks
Total: 25 marks
Assessment
Internal:
Assessment consists of average of two tests which should be conducted at
proper interval .
End Semester Theory Examination:
Question paper will comprise of 6 questions, each carrying 20 marks.
Total 4 questions need to be solved.
Question No.1 will be compulsory and based on entire syllabus wherein sub
questions can be asked.
Remaining ques tions will be randomly selected from all the modules.
Weightage of marks should be proportional to number of hours assigned to
each Module.
References
1. Mathematical Methods in Chemical Engineering, V.G. Jenson and G.V.
Jeffreys, Academic Press, 1970
2. Laplace transforms, Murray Spiegel, Schaum’s Outline Series, 1974
3. Complex variables, Murray Spiegel, Schaum’s Outline Series, 1964
4. Linear Algebra, Murray Spiegel, Schaum’s Outline Series, 1964
5. Probability and Statistics: Murray R. Spiegel, Schaum's Outline Series, 1965
6. Advanced Engineering Mathematics by Erwin Kreyszig , 9TH Edition,
Wiley India.
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University of Mumbai B. E. (Biotechnology) Rev 2016 Page 15
Course Code Course/Subject Name Credits
BTC302 Microbiology 4
Prerequisites:
Basic Knowledge of Living Cells
Course Objectives:
The course aims to develop skills of the Students in the area of Microbiology
particularly to identify microbes, their structure, their metabolism and their
industrial applications.
They will study various sterilization techniques and their effects.
This will be a prerequisite for a ll courses offered in Bioprocess Technology
Course outcomes:
Students will be able to carry out various microbiological techniques like
staining and isolation very well.
They would be able to identify microbes.
They would have detailed knowledge of various sterilization techniques,
which would be useful for other courses.
Module Contents Contact
Hours .
1 History and Scope of Industrial Microbiology:
Introduction: Discovery of Microbial world
The experiments of Pasteur; The discovery of Anaerobic
Life
Physiological significance of Fermentation; Pasteur and
Fermentation
The Era of discovery of Antibiotics; Growth of Industrial
fermentation 03
2 Classification of Microorganisms:
Types and general characteristics of microorganisms:
1) Bacteria - Archaebacteria, Actinomycetes, Rickettsia,
Mycoplasma, Chlamydia
2) Fungi – Molds and yeasts
3) Algae 4) Protozoa 5) Viruses
The classification of bacteria Species: The unit of
classification, New approaches to bacterial taxonomy,
Bacterial taxonomy the problems of taxonomic
arrangements, Bacterial phylogeny.
Aerobic and Anaerobic cultures
Microbial Pathogenesis:
Epidemiology of infectious diseases, Bacterial, Fungal,
Protozoal, Viral Diseases;
Bacterial invasion and colonization
Bacterial to xins- types and mode of action 08
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University of Mumbai B. E. (Biotechnology) Rev 2016 Page 16
3 Microbial Nutrition:
Nutritional requirements of microorganisms
Different types of media - Synthetic media, complex media
Selective media, differential media, enrichment media. 05
4 Microbial Growth:
Introduction: phases of growth
Growth curve
Kinetics of growth
Measurement of growth
Continuous &batch culture
Synchrony
Chemostat & turbidostat
Effects of solutes, temperature, ion concentration, oxygen,
hydrostatic pressure, heavy metal ions, and UV light on
microbial growth 05
5 Microbiological Techniques:
Sterilization and disinfection techniques,
Principles and methods of sterilization.
Physical methods - autoclave, hot -air oven, pressure
cooker, laminar airflow, filter sterilization.
Radiation methods – UV rays, gamma rays, ultrasonic
methods.
Chemical methods - Use of alcohols, aldehydes, fumigants,
phenols, halogens and hypochlorites. Phenol coefficient.
Isolation of pure culture techniques - Enrichment culturing,
dilution plating, streak - plate, spread -plate an d
micromanipulator.
Preservation of microbial cultures - sub culturing,
overlaying cultures with mineral oils, lyophilization, sand
cultures, storage at low temperature. 09
6 Antimicrobial Therapy:
Antimicrobial sensitivity tests.
Agents used in treating infection: Antibacterial, antiviral,
antiretroviral, antifungal, anti -protozoan & anti helminthes.
Resistance mechanism. 07
7 Water & Soil Microbiology:
Microbiological analysis of water purity -sanitary tests for
coliforms (presumptive test, confirmed test, competed
test), MPN test, defined substrate test, IMVIC test.
Soil microbiology - soil as a habitat for microorganisms,
physico -chemical properties of soil, microbial community
in soil, role of microorganisms in organic ma tter
decomposition. 07
Assessment
Internal:
Page 17
University of Mumbai B. E. (Biotechnology) Rev 2016 Page 17
Assessment consists of average of two tests which should be conducted at
proper interval .
End Semester Theory Examination:
Question paper will comprise of 6 questions, each carrying 20 marks.
Total 4 questions need to be solved.
Question No.1 will be compulsory and based on entire syllabus wherein sub
questions can be asked.
Remaining questions will be randomly selected from all the modules.
Weightage of marks should be proportional to number of hours assigned to
each Module.
References
1. Textbook of Microbiology ;R. Ananthnarayan, C. K. J. Panicker, Orient
Longman 6th Edition (2003)
2. General Microbiology, R.Y. Stanier, J.L. Ingraham, M.L.Wheelis and P.R.
Painter, Macmillian
3. Microbiology VI Edition, M.J. Pelczar, E.C.S. Chan and N.R. Kreig, Tata
McGraw Hill Microbiology by Prescott
4. Microbiology: An Introduction (9th Ed.) by Tortora GJ, Funke BR, and Case
CL, Pearson Education, 2008.
5. Industrial Microbiology, Casida, New Age International
6. Industrial Microbi ology, Prescott and Dunn, C.B.S. Publishers Principles of
Microbiology, R.M. Atlas, WMC. Brown Publisher.
7. Microbiology – Fundamentals and Application, 6th Ed. – Purohit, S.S.
(Agrobios)
8. Textbook of Microbiology, P.Charkborthy
9. General Microbiology Vol. II by Powar and Daginawala Himalaya Publ.
House 8th edition (2004)
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University of Mumbai B. E. (Biotechnology) Rev 2016 Page 18
Course Code Course/Subject Name Credits
BTC303 Cell Biology 4
Prerequisites:
Knowledge of basic terminology of cell and cell organelles
Knowledge of structure and function of prokaryotic and eukaryotic cell
Knowledge of different compartments of cell organelle.
Knowledge of cell division
Basic knowledge of cell events like photosynthesis, respiration
Course Objectives:
In this course, Students will explore the great diversity of all cellular form and
function.
Course emphasis is placed on the molecular mechanisms of cell metabolism,
growth, division, and communication.
This course is central to the cell biology and ser ves as the bridge between
foundational courses in the cell and advanced courses in the complexity of
sorting in the cell.
Course Outcome:
By the end of the course students should be able to grasp the fundamentals in
Understanding the molecular organizatio n of the cells, function and structure
of The different organelles including transport mechanisms for processes like;
Protein sorting, cell communication and flow of information and transport
across the unit membrane, cell signaling.
Students will have go od knowledge of cancer, its types and etiology. Students
will be able to appreciate all basic concepts which he may encounter in future
courses in biotechnology engineering.
Students will be ready for application of these concepts in the field of research
in biotechnology.
Module Contents Contact
Hours
1 Cytology:
Developmental history of cytology: Origin of cell, Robert
Hooke’s Experiment, Cell theory, Miller’s Experiment
Properties & Types of cells: Prokaryotic & Eukaryotic cell
Structure and function of cells such as Viruses, Bacteria,
Animal cells, Plant cells. 05
2 Cell cycle & cell death:
Cell cycle and its regulation: Cyclins, CDKs, Checkpoints
Cell division: Mitosis & Meiosis
Programmed cell death: Apoptosis, Extrinsic & Intrinsic
pathway
Apoptosis vs. Necrosis 05
3 Structural organization of cell and role of cell organelles in
sorting and intracellular transport: 08
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University of Mumbai B. E. (Biotechnology) Rev 2016 Page 19
Cell membrane: Function, Composition, Membrane proteins,
Fluid Mosaic model, Electrical properties of membrane,
Neurotransmission
Nucleus: Nuclear Envelop, Nuclear Pore Complex & its role
in nucleocytoplasmic exchange
Overview of endomembrane system: secretory and endocytic
pathway
Endoplasmic Reticulum: SER & RER, Protein synthesis on
membrane bound and free ribosomes, Protein Glycosylation
in ER & Golgi complex, Membrane biosynthesis in the ER
Golgi bodies: Movement of materials through the Golgi
complex
Structure & function of Cell wall, Mitochondria, Lysosomes
Structure and function of cytoskeleton:
(i)Microtubules – Structure & composition, MAPs, MTOCs,
Dynamic properties of microtubules, Overview of motor
proteins.
(ii)Microfilaments – Structure, Assembly & disassembly.
(iii)Intermediate filaments – Structure, Assembly &
disassembly, Types and function s.
4 Transport across cell:
Bulk transport: Exocytosis, Phagocytosis,
Endocytosis – Pinocytosis & Receptor mediated endocytosis
Mechanism of transport of substances through membrane:
(i) Active Transport – Ion pumps
(ii) Passive Transport – Diffusion, Osmosis, Facilitated
diffusion, Ion channels 05
5 Cellular communication:
General principles of cell communication: Types of adhesion,
CAMs
Extracellular matrix: Components – Collagen, Proteoglycans,
Fibronectin, Laminin
Interactions of cells with extracellular materials: Integrins,
Focal Adhesions & Hemidesmosomes
Interactions of cells with other cells: Selectins, IgSF,
Cadherins, Adherens junction, Desmosomes
Tight junctions
Gap junctions and plasmodesmata 07
6 Cell Signaling:
Types of intercellular signaling: Auto, Para & Endocrine
Overview of cellular signaling pathway
Various extracellular messengers and their receptors
Signal transduction by RTKs: Receptor dimerization, Protein
kinase activation, Activation of downstream signaling
pathways, Ending the response . Signaling by Insulin receptor 05
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University of Mumbai B. E. (Biotechnology) Rev 2016 Page 20
Term work
Term work shall consist of minimum eight tutorials from entire syllabus which
are to be given at regular intervals Batch wise.
Tutorials: 20 marks
Attendance: 05 marks
Total: 25 marks
Assessment
Internal:
Assessment consists of average of two tests which should be conducted at
proper interval .
End Semester Theory Examination:
Question paper will comprise of 6 questions, each carrying 20 m arks.
Total 4 questions need to be solved.
Question No.1 will be compulsory and based on entire syllabus wherein sub
questions can be asked.
Remaining questions will be randomly selected from all the modules.
Weightage of marks should be proportional to nu mber of hours assigned to
each Module.
References
1. Cell and Molecular biology: Concepts and Experiments, Gerald Karp, John
Wiley and sons Inc, 6th Edition (2010)
2. Molecular Cell Biology, H. Lodish et. al., W.H. Freeman & Co Ltd, 5th
Edition (2003)
3. Cell and Molecular Biology, DeRobertis; Lippincott Williams & Wilkins 8th
Edition (2001)
4. Molecular Biology of the Cell and the Hypercell with CDROM; Alberts, Bray;
Garland Publishing 1st Edition (1999)
5. Molecular Biology of the Cell with CDROM Alberts, Bruce; Johns on,
Alexander; Lewis, Julian 4th Edition (2005).
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University of Mumbai B. E. (Biotechnology) Rev 2016 Page 21
Course Code Course/Subject Name Credits
BTC304 Biochemistry 4
Prerequisites:
Knowledge of organic chemistry: functional groups and their reactions
Knowledge of living cell and its components
Course Objectives:
The major objective is to provide complete understanding of all the chemical
processes associated with living cells at the molecular level.
To ensure students have a strong grounding in structures and reactions of
biomolecules.
To introduce the m to the metabolic pathways of the major biomolecules.
To correlate biochemical processes with biotechnological applications.
Course outcomes:
The students will be able to understand and analyze the correlation between
biomolecules, their associated pathways and various biological processes
underlying the living systems.
Module Contents Contact
Hours
1 Introduction, aims and scope
Chemical foundations of Biology - Properties of water, acids,
bases and buffers, covalent bonds, Non -covalent interactions
in biological systems. 05
2 Biomolecules:
Classification, Structure and Functions of :
Carbohydrates:
Lipids
Proteins
Nucleic acids 10
3 Enzymes:
Working of Enzymes
Concept of Activation energy and transition state
Factors affecting enzyme activity - pH, Temperature,
Substrate & Enzyme Concentration 05
4 Vitamins and Hormones:
Vitamins: Classification, functions, role in metabolism,
vitamins as cofactors.
Hormones: Classification, endocrine glands, function and
mechanism of action of hormones. 05
5 Metabolism:
Carbohydrates - Glycolysis, TCA cycle
Lipids - Digestion by GI enzymes and breakdown of
Triglycerides: α, β, ω oxidation of fatty acids 15
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University of Mumbai B. E. (Biotechnology) Rev 2016 Page 22
Amino acids - decarboxylation, deamination &
transamination. Urea cycle; fate of amino acids (connection
to TCA)
Electron Transport Chain
Photophosphorylation - Photosystems, reaction centers,
pigments, cyclic and non -cyclic photophosphorylation, Z
pathway
6 Bioenergetics:
Laws of Thermodynamics
Concept of Enthalpy, Entropy
Energy rich compounds – ATP as energy currency 05
Assessment
Internal:
Assessment consists of average of two tests which should be conducted at
proper interval .
End Semester Theory Examination:
Question paper will comprise of 6 questions, each carrying 20 marks.
Total 4 questions need to be solved.
Question No.1 will be compulsory and based on entire syllabus wherein sub
questions can be asked.
Remaining questions will be randomly selected from all the modules.
Weightage of marks should be proportional to n umber of hours assigned to
each Module.
References
1. Nelson, D.L. and M.M. Cox, “Lehninger’s Principles of Biochemsitry”, 4th
Edition, W.H. Freemen & Co., 2005.
2. Murray, R.K., et al “Harper’s Biochemistry”, 23rd Edition, Prentice Hall
International, 1993
3. Lubert Stryer. 2007. Principles of Biochemistry. Freeman.
4. Voet and Voet. 2005. Biochemistry. Wiley.
5. D. Skoog, D. West, F.Holler, S. Crouch “Fundamentals of Analytical
Chemistry” 8th Edition, 2004. Thomson Brooks/ Cole
6. David T. Plummer, An Introduction to pra ctical biochemistry, Tata McGraw
Publishing Company Ltd.
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University of Mumbai B. E. (Biotechnology) Rev 2016 Page 23
Course Code Course/Subject Name Credits
BTC305 Unit Operations – I 3
Prerequisites:
Basic knowledge in physics, units and dimensions and thermodynamics
Course Objectives:
To impart the basic concepts of fluid statics and dynamics
To study the basic equations of fluid flow.
They should be comfortable with measurement of pressure or pressure drop.
To enable students to determine viscosity using method such as Stokes Law.
To study the different t ypes of size reduction equipment used in Industries.
To study about the metering and pumping of fluids.
Course Outcomes:
The student will have a thorough grounding on measurement of pressure drop,
velocity, flow rates etc. of fluids.
They can select pumps and would be able to calculate power requirement for
pumping as well as agitation operations.
They will be able to operate certain flow measurement devices and size
reduction equipment.
Module Content Contact
Hours
1 Introduction: Classification of fluids, Rheological behavior of
fluids & Newton’s Law of viscosity. Effect of temperature &
pressure on viscosity of fluids.
Fluid statics: Pascal's law ,Hydrostatic equilibrium,
Barometric equation and pressure measurement (problems) 05
2 Fluid Dynamics: Continuity Equation, Equation of motion,
Euler's equation of motion, Bernoulli’s equation (problems),
Bernoulli's equation for compressible fluids (isothermal and
adiabatic process) concept of Reynold's number, Laminar flow
in pipes, Tu rbulent flow in pipes, velocity and shear stress
distribution across pipe, Boundary layer formation and
separation of boundary layer. 07
3 Flow of Incompressible fluids: Relationship between skin
friction and wall shear, Fanning friction factor, friction factor
law for smooth pipes, Form friction, effect of roughness,
energy relationships, pipe fittings, major and minor losses in
pipe flow. (problems)
Flow measurements: Venturimeter, Orificemeter, Pitot tube,
Rotameter. (problems)
Pumping: Reciprocating pu mps, Rotary pumps, centrifugal
pumps (Characteristics, NPSH, and Cavitation) and blowers. 08
4 Particle Size distribution: Importance of particle size in
reactions, particle size, shape and mass distributions, 07
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University of Mumbai B. E. (Biotechnology) Rev 2016 Page 24
measurement and analysis, concept of average diameter.
(problems)
Screening: Screening equipment, capacity and effectiveness
of screen, effect of mesh size on capacity of screen. Particle
size analysis – mean diameter, derived diameter. Sieving -
cumulative method and differential method.
Transportati on and storage of solids: Studies performance
and operation of different conveyor systems like Belt, Screw,
Apron, Flight, pneumatic conveyor and elevators; Storage of
solids and discharge pattern from storage bin.
5 Size Reduction: Factors affecting s ize reduction,
comminution laws – Kick’s law, Rittinger’s law and Bond’s
law and their limitations. Crushing efficiency & power
consumption (problems)
Size reduction equipment: Grinder – Construction and
operation of Hammer mill, Ball mill (problems), Ultrafine
grinder – Fluid energy mill, Cutting machines: knife cutters. 04
6 Sedimentation: Free settling and Hindered settling, Stoke’s
law & Newton’s law regimes of settling. Cl arifiers and
thickeners, flocculation, batch Sedimentation (Kynch theory),
rate of sedimentation. 04
Assessment
Internal:
Assessment consists of average of two tests which should be conducted at
proper interval .
End Semester Theory Examination:
Question paper will comprise of 6 questions, each carrying 20 marks.
Total 4 questions need to be solved.
Question No.1 will be compulsory and based on entire syllabus wherein sub
questions can be asked.
Remaining questions will be randomly selected from a ll the modules.
Weightage of marks should be proportional to number of hours assigned to
each Module.
Text books
1. Dr. R. K. Bansal, “A Textbook of Fluid Mechanics & Hydraulic Machines”,
Laxmi Publications, 9th Edition, 2010.
2. R. S. Hiremath & A. Kulkarni. Mechanical Operations Vol. I.
3. McCabe, W.L, Smith J.C and Harriot, P., “Unit Operations in Chemical
Engineering”, McGraw Hill, Fourth Edition, 1984.
4. Narayanan C.M. & Bhattacharya B.C. “Mechanical operations for chemical
engineers”, Khanna.
References
1. Couls on, J.M., Richardson, J.F., “Chemical Engineering”, Volume 2, Third
Edition, Pergamon Press, 1977.
2. Badger and Bencharo, “Introduction to Chemical Engineering”. TMH,
3. Fluid Mechanics and Hydraulics by Suresh Ukarande, Ane Books, 2012.
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University of Mumbai B. E. (Biotechnology) Rev 2016 Page 25
Course Code Course/Subject Name Credits
BTC306 Process Calculations 4
Prerequisites:
Linear Algebra
Differential Equation
Course Objectives:
To study the laws regarding gas, liquid and vapor
To develop understanding about material balance and energy balances
To study the stoichiometry and thermodynamics of microbial growth and
product formation
Course outcomes:
The student will be able to understand basic application of various unit
operations & unit processes to industrial &theoretical problems
They will have a clear understanding of the various systems of units will be
able to do the conversion of units of one system to another.
They will be able to do basic calculations for biological systems & access the
property data from appropriate sources.
Module Content Contact
Hours
1 Units and dimensions: Systems of units, fundamental and
derived units, unit conversions, dimensional homogeneity and
dimensional analysis problems. Conversion of units
Chemical arithmetic: Mole concept, atomic weight, molecular
weight and equivalent weight - methods of determination.
Chemical composition: Methods of expressing compositions
of mixtures and solutions - mole percent, mass percent,
volume percent, molarity, molality, normality etc.
P-V-T behavior of pure liquids - Gas laws, real and ideal
gases, equation of state, critical properties, properties of gas
mixtures - Dalton’s laws, Amagat’s law -Average molecular
weight and density problems.
Biochemical stoichiometry: Limiting and excess reactants -
conversion , degree of completion, sel ectivity, yield problems. 07
2 Fundamentals of material balances - Law of conservation of
mass - Types of material balances, material balance with
recycle bypass and purge streams 07
3 Material Balance for process involving chemical reaction,
Calculations using Psychrometric chart; Humidity and
saturation 07
4 Fundamentals of energy balances, Law of conservation of
energy, Heat capacity, sensible heat, latent heat, calculation of
enthalpy changes. General energy balance equation; Energy
balance calculations with and without reactions, Energy 07
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University of Mumbai B. E. (Biotechnology) Rev 2016 Page 26
balance for fermentation and downstream processing
problems.
5 Stoichiometry of microbial growth and product formation,
Growth , Stoichiometry and elemental balances, respiratory
quotient, degree of reduction, Yield and maintenance
coefficients, Oxygen consumption in aerobic microbial
cultures. Theoretical Oxygen demand - problems.
Bioc hemical energetics: Metabolic reaction coupling,
energetics of metabolic processes (respiration and
biosynthesis) . Transport across cell membranes,
Thermodynamics of microbial growth, Heat generation in
microbial cultures problems. 07
Term work
Term work shall consist of minimum eight tutorials from entire syllabus which
are to be given at regular intervals Batch wise.
Tutorials: 20 marks
Attendance: 05 marks
Total: 25 marks
Assessment
Internal:
Assessment consists of average of two tests which should be conducted at
proper interval .
End Semester Theory Examination:
Question paper will comprise of 6 questions, each carrying 20 marks.
Total 4 questions need to be solved.
Question No.1 will be co mpulsory and based on entire syllabus wherein sub
questions can be asked.
Remaining questions will be randomly selected from all the modules.
Weightage of marks should be proportional to number of hours assigned to
each Module.
References
1. David M. Himmelblau. 1989. Basic Principles and Calculations in Chemical
Engineering. Prentice Hall of India (P) Ltd.
2. Hougen, K. M. Watson and R. A. Ragatz. 1970. Chemical Process Principles,
Part - I, John Wiley and Asia Publishing Co.
3. Bhat B.I and S. M. Vora, 200 5. Stoichiometry. Tata McGraw Hill.
4. Richard Felder and Ronald W. Rausseau. 1986. Elementary Principles of
Chemical Processes. John Wiley & Sons.
5. Doran P.M, Bioprocess Engineering Principles, Academic Press
6. Bailey G.E and Ollis D.F, Bioprocess Engineering F undamentals McGraw Hill
7. Shuler M.L and Kargi F, Bioprocess Engineering - Basic Concepts, Pearson
Education
8. Segel I.H, Biochemical Calculations, John Wiley
9. Blanch H.W and Clark D.S, Biochemical Engineering Marcel Dekker Inc.
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University of Mumbai B. E. (Biotechnology) Rev 2016 Page 27
Course Code Course/Subject Name Credits
BTL30 1 Microbiology Lab 1.5
List of Experiments Suggested:
Study of different equipments - Bunsen burner, water bath, Autoclave, Laminar
air flow, Incubator, Hot air oven, Centrifuge, and Refrigerator.
Study of Microscope - Compound Microscope & its parts. Use of oil
Immersion objective.
Preparation of medium -nutrients broth, nutrient agar, agar slant.
Staining: Simple, Differential staining methods, Capsule, Endospore; Study of
shape and arrangement of bacterial cells
Isolation of microorganism by Pure Culture Techniques.
Effect of disinfectants on microbial flora
Isolation and identification of microorganisms from different sources – soil,
water and milk
Antibiotic sensitivity assay
Effect of different parameters on bacterial growth (pH, temper ature & UV
irradiation)
Culture of aerobic &anaerobic bacteria
Effect of TDP & TDT on bacterial growth
Filter paper disc methods for evaluation of antiseptics
Study of growth curve of E. coli
Bacterial colony counting using Haemocytometer
Practical Examin ation
Duration for practical examination would be the same as assigned to the
respective lab per week.
A student becomes eligible for practical examination after completing a
minimum of eight experiments out of ten experiments.
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University of Mumbai B. E. (Biotechnology) Rev 2016 Page 28
Course Code Course/Subject Name Credits
BTL30 2 Biochemistry Lab 1.5
List of Experiments Suggested:
Preparations of solutions –molar, normal, ppm, percent
Study of pH meter and preparation of buffers
Study of Beer and Lambert’s Law and absorption maxima
Glucose estimation by DNSA method
Protein estimation by Biurette Test
DNA estimation by DPA method
RNA estimation by Orcinol method
Estimation of Vitamin C by Iodometry
Extraction and separation of plant pigment by paper chromatography
TLC of Fatty acids
Study of Enzyme Activity
Estimation of Lipids
Practical Examination
Duration for practical examination would be the same as assigned to the
respective lab per week.
A student becomes eligible for practical examination after completing a
minimum of eight experiments out of ten experiments.
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University of Mumbai B. E. (Biotechnology) Rev 2016 Page 29
Course Code Course/Subject Name Credits
BTL303 Unit Operations - I Lab 1.0
List of Experiments Suggested:
Viscosity by Stoke’s Law
Venturimeter
Orificemeter
Flow through Helical coil
Reynold's Apparatus.
Bernoulli’s apparatus
Sieve analysis
Screen effectiveness
Major and Minor losses
Ball mill
Hammer mill
Sedimentation
Centrifugal pumps
Page 30
University of Mumbai B. E. ( Biotechnology) Rev 2016 Page 30
University of Mumbai
Program Structure for B.E. Biotechnology (Revised 2016 )
S.E. Semester IV (w.e.f 2017 -2018)
Course
code Course Name
Teaching Scheme
(Contact Hours) Credits Assigned
Theory Practical Tutorial Theory Practical Tutorial Total
BTC401 Applied Mathematics -IV 3 - 1 3 - 1 4
BTC402 Molecular Genetics 3 - 1 3 - 1 4
BTC403 Fermentation Technology 4 - - 4 - - 4
BTC404 Analytical Methods in
Biotechnology 4 - - 4 - - 4
BTC405 Immunology and
Immunotechnology 3 - 1 3 - 1 4
BTC406 Unit Operations -II 3 - - 3 - - 3
BTL401 Fermentation Technology
Lab - 3 - - 1.5 - 1.5
BTL402 Analytical Methods in
Biotechnology Lab - 3 - - 1.5 - 1.5
BTL403 Unit Operations -II Lab - 2 - - 1 - 1
Total 20 8 3 20 4 3 27
Course
code Course Name
Examination Scheme
Theory
Term
Work Pract/
Oral Oral Total Internal Assessment End
Sem
Exam Exam
Duration
(in hrs) Test 1 Test 2 Avg
BTC401 Applied Mathematics -IV 20 20 20 80 3 25 - - 125
BTC402 Molecular Genetics 20 20 20 80 3 25 - - 125
BTC403 Fermentation Technology 20 20 20 80 3 - - - 100
BTC404 Analytical Methods in
Biotechnology 20 20 20 80 3 - - - 100
BTC405 Immunology and
Immunotechnology 20 20 20 80 3 25 - - 125
BTC406 Unit Operations -II 20 20 20 80 3 -- - - 100
BTL401 Fermentation Technology
Lab - - - - 3 -- 25 - 25
BTL402 Analytical Methods in
Biotechnology Lab - - - - 3 -- 25 - 25
BTL403 Unit Operations -II Lab - - - - - -- - 25 25
Total 120 480 - 75 50 25 750
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University of Mumbai B. E. ( Biotechnology) Rev 2016 Page 31
Course Code Course/Subject Name Credits
BTC401 Applied Mathematics IV 4
Prerequisites:
Vector Calculus :- Multiple Integral, Partial differentiation, basic knowledge of
vectors and their products, Knowledge of spherical and cylindrical coordinate
system.
Partial Differential Equation: - Integration, Know2ledge of partial derivatives.
Course Objectives:
The sy llabus/module aims to introduce the above topics (to the Learner) so as
to equip the learner with mathematic tools to effectively model, analyze and
find the solution of various problems in Chemical Engineering and
Biotechnology processes.
One can use vect or formation and calculus together to describe and solve many
problems in two/three dimension. The Fourier Transform and PDE module
does the ground work for the techniques required to solve and find the answer
for various physiochemical problems.
Course Outcomes:
It is expected that the learner will develop the proactive approach towards the
selection of methods to a solution of Chemical Engineering and Biotechnology
problems coming across while studying higher level of the Course . (Example:
Flow of Liqui d through Pipes/Gases etc.)
Module Contents Contact
Hours
01 Fourier Series
Expansion of functions in any interval (a, b ). Half range
expansion; Complex form; Parseval’s identity theorem;
Orthogonal and Orthonormal functions. NO PROOFS
REQUIRED. 09
02 Fourier Integrals and Fourier Transform; sine & cosine
Integrals, sine & cosine transforms, complex transforms.
NO PROOFS REQUIRED. 10
03 Partial Differential Equations
Elliptic, Parabolic & Hyperbolic Equations; Laplace’s
equation; One dimensional Heat & Wave Equation, Two
Dimensional wave equation. (ONLY NUMERICAL
PROBLEMS. NO PROOFS REQUIRED). 10
04 Vector Integration
Green’s Theorem in the plain; Conservative & Solenoidal
Fields . Green’s Theorem in the plain; Conservative , Gauss
Divergence Theorem, Stokes’ Theorem. (ONLY
NUMERICAL PROBLEMS. NO PROOFS REQUIRED). 10
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University of Mumbai B. E. ( Biotechnology) Rev 2016 Page 32
Term work
Term work shall consist of minimum eight tutorials from entire syllabus which are
to be given at regular intervals Batch wise.
Tutorials: 20 marks
Attendance: 05 marks
Total: 25 marks
Assessment
Internal:
Assessment consists of average of two tests which should be conducted at
proper interval .
End Semester Theory Examination:
Question paper will comprise of 6 questions, each carrying 20 marks.
Total 4 questions need to be solved.
Question No.1 will be compulsory and based on entire syllabus wherein sub
questions can be asked.
Remaining questions will be randomly selected from all the modules.
Weightage of marks should be proportional to number of hours assigned to
each Module
References
1. Advanced Engineering Mathematics by Erwin Kreyszig , 9TH Edition, Wiley
India.
2. Schuam’s outline series in Fourier series.
3. Schuam’s outline series in partial dif ferential equations.
4. Partial differential equations Vol 1 by Rutherford Aris.
Page 33
University of Mumbai B. E. ( Biotechnology) Rev 2016 Page 33
Course Code Course/Subject Name Credits
BTC402 Molecular Genetics 4
Prerequisites:
Knowledge of Cell and its components
Knowledge of Biomolecules and their functions
Knowledge of Cellular Metabolism
Course Objective:
Understand the Central Dogma of gene expression
Explain the foundations of Mendelian genetics and chromosomal theory and
apply these, with appropriate terminology, to contemporary concepts in
genetics.
Understand the redundant and universal qualities of the genetic code and how it
is used to determine the amino acid sequence of a polypeptide.
Describe the processes of transcription and translation in both prokaryotes and
eukaryotes at the molecular level.
Describe how prokaryotes control their gene expression through positive and
negative regulatory mechanisms.
Course outcome:
Students will get knowledge of molecular biology and genetics of Prokaryotic
and eukaryotic organisms .
Students will get insight on Replication, Transcription and translation processes
in prokaryotes and e ukaryotes, various mutations, their Repair mechanisms.
Genetic syndromes.
Module Contents Contact
Hours
01 Structure of Nucleic Acid: 04
• DNA, RNA: mRNA,tRNA, rRNA,
• Denaturation and Renaturation of DNA,
• Tm, GC content from Tm,
• Complexity of DNA, Cot curves
• Satellite DNA: Repetitive DNA, SNP, STR
02 Mendelism and its extensions 06
• Mendel’s Laws , problems based on his laws
• Linkage and Crossing Over
• Multiple allelism
• ABO blood group inheritance
03 Cytogenetics 04
• International System for Human Chromosome Nomenclature
• Mechanisms of numerical and structural chromosomal
aberrations
• Chromosomal and non -chromosomal basis of sex determination
• Syndromes – Down’s, Turner, Cri Du Chat, Klinfelter
Transposons
Fluorescence in -situ hybridization technique and applications
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University of Mumbai B. E. ( Biotechnology) Rev 2016 Page 34
04 DNA Replication and Repair: 06
• Prokaryotic and Eukaryotic DNA replication mechanism -
Enzymes and accessory proteins involved in DNA replication ,
• DNA Mutations: Types of Mutations and Mutagens
• DNA Repair Mechanism – Excision, recombinational, SOS,
Photo reactivation , Mis -match repair
05 Transcription 05
• Relationship between Genes and Proteins
• Prokaryotic transcription
• Eukaryotic transcription: Eukaryotic RNA Polymerases,
Transcription of protein coding genes, Production of mature
mRNAs in Eukaryotes - 5' Modification , 3' Modification ,
Intron splicing, RNA Editing
06 Translation 05
•
•
•
•
The nature of Genetic Code
Synthesis of aminoacyl -tRNA, Ribosomal RNA genes
Mechanism of initiation, elongation and termination of
Translation in bacteria
Co-and post -translational modifications of proteins
07 Regulation of gene expression 05
• Operon theory
• lac Operon: Structure, Regulation of Lac genes, Positive
control of Lac Operon
• trp Operon: Gene organization of the tryptophan biosynthetic
genes, Regulation of the trp operon, attenuation
• ara operon: structure and regulation of ara operon
Term work
Term work shall consist of minimum eight tutorials from entire syllabus which are
to be given at regular intervals Batch wise.
Tutorials: 20 marks
Attendance: 05 marks
Total: 25 marks
Assessment
Internal:
Assessment consists of average of two tests which should be conducted at
proper interval .
End Semester Theory Examination:
Question paper will comprise of 6 questions, each carrying 20 marks.
Total 4 questions need to be solved.
Question No.1 will be compulsory and based on entire syllabus wherein sub
questions can be asked.
Remaining questions will be randomly selected from all the modules.
Weightage of marks should be proportional to number of hours assigned to
each Module
Page 35
University of Mumbai B. E. ( Biotechnology) Rev 2016 Page 35
References
1. Molecular Biolo gy; David Freifelder, Narosa Publishing House , 2nd edition
(2004) .
2. Microbial Genetics; David Freifelder, Narosa Publishing House, 2nd edition
(2004) .
3. Principles of Gene Manipulations; S. B. Primrose, R. M. Twyman, R. W. old,
Blackwell Science,6th Edition (2003)
4. Genetics: A molecular Approach, 2nd edition; Peter J Russell
5. Gene VIII; Benjamin Lewin; Oxford Univ. Press, 8th edition (2004)
6. Cell and Molecular biology: Concepts and Experiments, Gerald Karp, John
Wiley and sons Inc, 6th Edition (2010)
Page 36
University of Mumbai B. E. ( Biotechnology) Rev 2016 Page 36
Course Code Course/Subject Name Credits
BTC403 Fermentation Technology 4
Prerequisites:
Knowledge of microbiology
Course Objectives: To gain broad knowledge on
Role of microorganisms in fermentation
The various fermentation technologies used
Production of important products through fermentation
Course Outcomes:
Appreciate the use of microorganisms for the production of value added
commodities.
Understand the working of a fermentation system.
To describe key industrial bioprocesses, from the traditional to the recently
evolved.
Integrate biological and engineering principles involved in the production and
recovery of commercial products.
Develop critical thinking skills and learn to employ a quantitative, scientific
approach towards conversion of biological materials to value added products.
Module Contents Contact
Hours
01 Introduction to fermentation 04
History and development of fermentation, general
requirements of the fermentation, range of fermentation
processes, parts of a fermentation process - upstream and
downstream processing, aerobic and anaerobic fermentation,
solid state and submerged fermentation.
02 Introduction to Microbial Growth Kinetics 05
Batch culture (Quantifying cell concentration, Growth patterns
and Kinetics), Continuous culture, Comparison of batch and
continuous cultures in industrial processes, Fed batch culture,
Examples of use of fed batch cultures.
03 Isolation, preservation and improvement of industrial 07
microorganisms
Isolation methods utilizing selection of the desired
characteristics
Isolation methods not utilizing selection of the desired
characteristics
The preservation of industrially important
microorganisms
Improvement of industrial microorganisms
The selection of induced mutants synthesizing
improved levels of products
The use of rDNA techniques
04 Regulatory Mechanisms controlling the catabolic and 03
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University of Mumbai B. E. ( Biotechnology) Rev 2016 Page 37
anabolic pathways of microbes Induction, carbon catabolite
repression, crab tree effect, feedback inhibition and repression
05 Media for industrial fermentations & sterilization 06
Introduction, Typical media, Energy sources, Carbon sources,
Nitrogen sources, Buffers, Oxygen requirements, Antifoams,
Medium optimization, Medium sterilization: The design of
batch sterilization processes, The design of continuous
sterilization processes, Sterilization of the fermenter, feeds and
air, Filter sterilization
06 The development of inocula for industrial fermentations 04
The development of inocula for yeast , bacterial and fungal
processes, The aseptic inoculation of plant fermenters
07 Aeration and agitation 04
The oxygen requirements and supply of industrial
fermentations, Determination of KLa, Factors affecting KLa
values, The balance between oxygen supply and demand
08 Design of fermenter 04
Basic function of a fermenter for microbial or animal cell
culture, body construction, various parts of a fermenter
09 Important products through Fermentation 08
Organic acids: citric and acetic acid; enzymes : amylase,
protease, lipase; antibiotics: penicillin; vitamins: vitB12;
aminoacids: lysine, Glutamic acid ; organic solvents: ethanol,
acetone butanol; alcoholic breverages: wine, beer; biomass :
bakers yeast; biofertilizers; biopesticides; biosurfactant;
steroid transformation; biopolymers
Assessment
Internal:
Assessment consists of average of two tests which should be conducted at
proper interval .
End Semester Theory Examination:
Question paper will comprise of 6 questions, each carrying 20 marks.
Total 4 questions need to be solved.
Question No.1 will be compulsory and based on entire syllabus wherein sub
questions can be asked.
Remaining questions will be randomly selected from all the modules.
Weightage of marks should be proportional to number of hours assigned to
each Module
References
1. Principles of Fermentation Technology – Stanbury P.F., Whitaker A, Hall S. J.
2. Bioprocess Engineering: Basic concepts – Shuler M.L., Kargi F. (PHI)
3. Bioprocess Engineering Principles – Doran Pauline M. (Elsevier Pub.)
4. Biotechnology: A textbook of Industrial Microbiology - Cruger, W. and A.
Cruger
5. Introduction to Biochemical Engineering - DG Rao, 2005, Tata M cGraw -Hill,
New Delhi
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University of Mumbai B. E. ( Biotechnology) Rev 2016 Page 38
Course Code Course/Subject Name Credits
BTC404 Analytical Methods In Biotechnology 4
Pre-requisites :
Basic knowledge of Physical and Analytical Chemistry
Knowledge of various types of spectra
Knowledge of Biomolecules and their properties
Course Objective:
To study the various analytical techniques used in Biotechnology.
Course outcomes:
The students will be capable of handling different instruments in the
laboratory.
They would be able to compare different separation techniques and use them
effectively in research work
Module Contents Contact
Hours
01 Centrifugation: 08
General principle - sedimentation velocity, sedimentation
equilibrium
Types of centrifuges, preparative and analytical centrifugation,
differential centrifugation, density gradient methods
Applications
02 Chromatographic Techniques: 12
Introduction to chromatography, General principles
Planar Chromatography: Thin layer chromatography, paper
chromatography
Column chromatography –columns, stationary phases. Packing
of columns, application of sample, column development, fraction
collection and analysis.
Partition chromatography, Adsorption chromatography
Affinity Chromatography, Ion Exchange Chromatography,
Chromato focussing, Size exclusion chromatography.
Gas Chromatography, HPLC: Principle & Components:
pumping systems, detectors systems
Applications
03 Electro kinetic methods of separation: 09
Electrophoresis: General principle and application, factors
affecting electrophoresis – voltage, current, resistance, buffer,
composition, concentration, pH.
Agarose Gel electrophoresis
SDS-PAGE – Native and denaturing gels, gradient gels,
discontinuous buffer system
Two dimensional gel electrophoresis
Isoelectric focusing
Capillary electrophoresis
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University of Mumbai B. E. ( Biotechnology) Rev 2016 Page 39
Immuno electrophoresis
04 Spectroscopy: 08
Spectroscopic Techniques; Beers Lamberts law, molar and
extinction coefficient, limitations of Beers Lamberts law
Visible and UV Spectrophotometry; Principles,
Instrumentation and applications
05 Radio isotopic techniques: 08
Use of radioisotopes in life sciences, radioactive labelling,
principle and application of tracer techniques
Detection and measurement of radioactivity using ionization
chamber, proportional chamber, Geiger -Muller and Scintillation
counters, Autoradiography
Applications
Assessment
Internal:
Assessment consists of average of two tests which should be conducted at
proper interval .
End Semester Theory Examination:
Question paper will comprise of 6 questions, each carrying 20 marks.
Total 4 questions need to be solved.
Question No.1 will be compulsory and based on entire syllabus wherein sub
questions can be asked.
Remaining questions will be randomly selected from all the modules.
Weightage of marks should be proportional to number of hours assigned to
each Module
References
1. Wilson K and Goulding K.H., A biologist’s guide to Principles and
Techniques of Practical Biochemistry.
2. Willard and Merrit, Instrumental Methods and Analysis
3. Ewing GW, Instrumental Methods of Chemical analysis.
4. Robert. M. Silverstein et al, Spectrometric identification of Organic
Compounds, 7th Edition, 1981.
5. Vogel’s, Text Book of Quantitative Chemical Analysis, 6th Edition, 2004.
6. John A. Adamovic, Chromatographic Analysis of Pharmaceuticals, 2nd
Edition.
7. Raymond P. W. Scott, Techniques and Practice of Chromatography –Vol. 70.
8. Sethi P.D, Dilip Charegaonkar, Chromatography –2nd Edition.
9. Niessen W. M. A., Van Der Greef J, Liquid Chromatography – Mass
Spectrometry, Vol. 58.
10. Kalsi. P.K, Spectroscopy of Organic Compounds .
11. Hanes, Gel Electrophoresis of Proteins - A Practical Approach,
12. Hamilton R. J. and Sewell P. A, Introduction to High Performance Liquid
Chromatography
13. Gordon M. Message, Practical aspects of Gas Chromatography and Mass
Spectrometry, John Wiley and Sons, Ne w York. 1984
14. Chapman J.M and G. Ayrey, The use of radioactive isotopes in the life
sciences, George Allen and Unwin Ltd., London.
15. Biophysical chemistry by Upadhyay, Upadhyay and Nath, Himalaya
publication house.
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University of Mumbai B. E. ( Biotechnology) Rev 2016 Page 40
Course Code Course/Subject Name Credits
BTC405 Immunology and Immunotechnology 4
Prerequisites:
Knowledge of anatomy and physiology of human body
Knowledge of blood components and blood cells
Knowledge of lymphatic system
Knowledge of principle of immune response and vaccine
Knowledge of history and basic terminology in immunology
Objectives
To learn about various basic terminology in immunology
To have knowledge of immune system in detail
To describe the interaction of antigens and antibodies in antibody mediated
and cell -mediated immune responses.
To make familiar with the techniques involved in antigen and antibody
reactions
To understand the concepts and principle of immunoassay techniques in
routine diagnosis, research
To learn principle and types of vaccines
Outcomes:
Student can define innate and adaptive immunity
Student can define the characteristics of antigens
Student can define the characteristics of antibodies
Student can describe cellular cooperation in antibody and cell mediated
immune responses
Student can define antigen antibody interaction
Student can describe Production of Monoclonal Antibodies and Recombinant
Vaccines.
Module Contents Contact
Hours
01 Introduction to immune system 06
Innate and adaptive immunity
Cells and organs of the immune system
Primary and secondary immune responses;
Cell mediated and humoral response
02 Antigens & Antibodies 04
Antigens
Antibodies and T cell receptors: Antigens, Structure and
function of immunoglobulin,
B and T cell receptors
03 Generation and regulation of immune responses 08
Antigen processing and presentation
MHC -restriction; Cytokines
T Cell Maturation, activation and Differentiation B Cell
Generation, activation and differentiation
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University of Mumbai B. E. ( Biotechnology) Rev 2016 Page 41
Clonal selection and immunological memory
Complement system, classical, alternative and MBL
pathway
Cell mediated cytotoxic responses
Regulation of immune responses; Immunological tolerance
04 Antigen -antibody Reactions 07
Strength of Antigen -Antibody Reactions
In Vivo Antigen -Antibody Reactions, In Vitro Antigen -
Antibody Reactions
Precipitation (In Fluid and In Gel Immuno electrophoresis),
Agglutination (Heamagglutination, Bacterial agglutination,
Passive agglutination and Agglutination Inhibition).
Radio immuno Assay (RIA)
Enzyme Linked Immunosorbant Assay (ELISA),
Western Blot
Immune Fluorescence
Immunoprecipitation
05 Disorders of Human Immune System 06
Primary and secondary immunodeficiency; Autoimmune
disorders; Hypersensitive reactions; Cytokine related
diseases
06 Production of Monoclonal Antibodies and Recombinant
Vaccines. 05
Monoclonal antibody, polyclonal antibody. Production of
Monoclonal antibodies - Definition, production,
applications.
Vaccines - Definition, recombinant vector vaccines,
DNA vaccines ,Multivalent subunit vaccines, minicell
vaccines, conjugate vaccines
Term work
Term work shall consist of minimum eight tutorials from entire syllabus which
are to be given at regular intervals Batch wise.
Tutorials: 20 marks
Attendance: 05 marks
Total: 25 marks
Assessment
Internal:
Assessment consists of average of two tests which should be conducted at
proper interval .
End Semester Theory Examination:
Question paper will comprise of 6 questions, each carrying 20 marks.
Total 4 questions need to be solved.
Question No.1 will be co mpulsory and based on entire syllabus wherein sub
questions can be asked.
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University of Mumbai B. E. ( Biotechnology) Rev 2016 Page 42
Remaining questions will be randomly selected from all the modules.
Weightage of marks should be proportional to number of hours assigned to
each Module
References
1. Essential Immunol ogy: Ivan Roitt.
2. Kuby Immunology: Golds by, Kindt and Osborne.
3. Immunology: Roitt, Brostoff, Mole.
4. Introductory Immunology : Huw Davies
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University of Mumbai B. E. ( Biotechnology) Rev 2016 Page 43
Course Code Course/Subject Name Credits
BTC406 Unit Operation - II 3
Pre-requisites :
An understanding of differential equations and basic physical concepts, units
and dimensions
Course Objectives:
To study the basics of Heat and Mass Transfer
To develop understanding about the application of Heat and Mass transfer in
Bio processing .
To calcu late the size of heat transfer equipments, for a known quantity of raw
material.
To apply energy balance.
To understand the role of diffusion, drying &distillation in the processes. To
apply material balance.
To design equipments in which heat &mass transf er occurs.
Course outcomes:
The student will be able to understand basic application of various unit
operations &unit processes to industrial &theoretical problems
They will have a clear understanding of the theories of Heat and Mass
transfer which are used for modeling.
They will be able to do design the fermenter and Bioreactors using the
models developed.
Module Contents Contact
Hours
01 Introduction: Various modes of heat transfer Viz. Conduction,
Convection and Radiation. 07
Conduction: Fouriers law, Steady state unidirectional heat flow
through single and multiple layer slabs, Cylinders and spheres
for constant and variable thermal conductivity.
Insulation: Properties of insulation materials, Types of
insulation, Critical and Optimum thickness of insulation
Extended Surfaces: Fins – Types of fins, Derivation of fin
efficiency for longitudinal fins, Fin effectiveness. Elementary
treatment of unsteady state heat conduction. Problems
02 Convection: Individual and overall heat transfer coefficient,
LMTD, LMTD correction factor. 08
Dimensionless numbers, - Dimensional analysis, Empirical
correlation for forced and natural convection.
Analogy between momentum and heat transfer – Reynolds,
Coulburn and Prandtl analogies. Problems
Heat Transfer with Phase Change: Boiling phenomena,
Nucleate and film boiling, Condensation – Film and Drop wise
condensation, Nusselts equations.
03 Radiation: Properties and definitions, Absorptivity, Reflectivity,
Emissive power and intensity of radiation, Black body radiation, 08
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University of Mumbai B. E. ( Biotechnology) Rev 2016 Page 44
Gray body radiation,
Stefen – Boltzmann law, Wien’s displacement law, Kirchoffs
law, View factors, Radiation between surfaces - different shapes,
Radiation involving gases and vapours, Radiation shields.
Heat Transfer Equipment: Double pipe heat exchangers, Shell
and tube heat exchangers – Types of shell and tube heat
exchangers, Condenser – types of condensers. Design of heat
exchanger.
Evaporators: Types of evaporators, performance of tubular
evaporator – Evaporator capacity, Evaporator economy,
Multiple effect evaporator
04 Diffusion: Molecular diffusion in fluids, Diffusion coefficient,
Flick’s Law of diffusion, Dependence of diffusion coefficient
on temperature, pressure and compos ition, Diffusion in multi -
component gas mixtures. Diffusion in solids: Molecular,
Knudsen &surface diffusion Inter - phase mass transfer, Mass
transfer coefficients ,Diffusion between phases , Equilibrium
solubility of gases in liquids, Mass Transfer theori es, Mass
transfer in fluidized beds , flow past solids and boundary layers,
Simultaneous heat and mass transfer. 07
05 Mass Transfer in Bioprocess Operations: Role of Diffusion in
Bio processing , Oxygen Uptake in Cell Culture, Factors
affecting cellular oxygen demand, oxygen transfer from gas
bubble to cell, oxygen transfer in fermenters, sparging stirring
and medium properties, anti foaming agents, temperature, gas
pressure and oxygen partial pressure, presence of cells,
measuring dissolved oxygen concentration, estimating oxygen
solubility, effect of oxygen partial pressure, effect of
temperature, effect of solutes, mass transfer correlations,
measurement of kLa, oxygen balance method, dynamic method,
sulphite oxidation method, oxygen transfer in l arge vessels. 05
Assessment
Internal:
Assessment consists of average of two tests which should be conducted at
proper interval .
End Semester Theory Examination:
Question paper will comprise of 6 questions, each carrying 20 marks.
Total 4 questions need to be solved.
Question No.1 will be compulsory and based on entire syllabus wherein sub
questions can be asked.
Remaining questions will be randomly selected from all the modules.
Weightage of marks should be proportional to number of hours assigned to
each Module
References
1. Robert E Treybal, Mass Transfer Operations, McGraw Hill Third Edition
2. Diffusion: Mass Transfer in Fluid System (Cambridge series in Chemical
Engineering) by E.L.Cussler”
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University of Mumbai B. E. ( Biotechnology) Rev 2016 Page 45
3. McCabe & Smith, Unit Operations in Chemical Engineering, 6th Edition,
McGraw Hall, 2001.
4. Coulson and Richardson, Chemical Engineering Vol I, 4th Edition, Pergmon
5. Press , 1998 .
6. Badger & Banchero, Introduction to Chemical Engineering, TMH 6th Reprint,
1998.
7. Doran P.M, Bioprocess Engineering Principles, Academic Press
8. Bailey G.E and Ollis D.F, Bioprocess Engineering Fundamentals McGraw
Hill
9. Shuler M.L and Kargi F, Bioprocess Engineering - Basic Concepts, Pearson
Education
10. Blanch H.W and Clark D.S, Biochemical Engineering Marcel Dekker Inc.
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University of Mumbai B. E. ( Biotechnology) Rev 2016 Page 46
Course Code Course/Subject Name Credits
BTL40 1 Fermentation Technology Lab 1.5
List of Experiments Suggested:
Alcohol production by baker’s yeast
Isolation and preservation of microorganism of commercial importance
Cell immobilization technique by immobilizing yeast cells in calcium alginate
beads.
Production of citric acid by A.niger
Hydrolysis of sucrose by immobilized yeast cells
Determination of cell mass by different methods ( dry weight method, density
method and haemocytometer me thod)
Estimation of carbohydrates from fermentation media.
Production of amylase
Isolation of auxotrophic mutants of industrially important microorganisms
Study of substrate utilization kinetics of the organism
Study the set up of various types of bioreactors
Introduction to fermentor .
Practical Examination
Duration for practical examination would be the same as assigned to the
respective lab per week.
A student becomes eligible for practical examination after completing a
minimum of eight experiments out of ten experiments.
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University of Mumbai B. E. ( Biotechnology) Rev 2016 Page 47
Course Code Course/Subject Name Credits
BTL40 2 Analytical Methods In Biotechnology Lab 1.5
List of Experiments Suggested:
Chromatography of amino acids and sugars
Agarose gel electrophoresis
SDS-PAGE, Native PAGE
Iso-electric Focussing
Centrifugation
Density gradient Centrifugation
Affinity chromatography
Ion exchange chromatography
Gel filtration chromatography
UV-Visible spectrophotometer
Thin Layer Chromatography
Paper Chromatography
Practical Examination
Duration for practical examination would be the same as assigned to the
respective lab per week.
A student becomes eligible for practical examination after completing a
minimum of eight experiments out of ten experiments.
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University of Mumbai B. E. ( Biotechnology) Rev 2016 Page 48
Course Code Course/Subject Name Credits
BTL40 3 Unit Operations - II Lab 1
List of Experiments Suggested:
Plate type H.E
Natural convection
Forced convection
Critical Heat flux
Emissivity
Heat transfer through composite wall
Shell & Tube H.E
k of insulating material
Vapor -liquid equilibrium
Diffusivity of a liquid
Diffusion through porous solids
Determination of Mass transfer coefficients in Gas Liquid sysytem by
evaporation
Determination of Mass transfer coefficients in Liquid Liquid system.
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University of Mumbai B. E. (Biotechnology) Rev 2016 Page 49
University of Mumbai
Program Structure for B.E. Biotechnology (Revised 2016 )
T.E. Semester V (w.e.f 20 18-2019 )
Course
code Course Name Teaching Scheme
(Contact Hours) Credits Assigned
Theory Practical Tutorial Theory Practical Tutorial Total
BTC501 Bioinformatics 4 - - 4 - - 4
BTC502 Genetic Engineering 4 - - 4 - - 4
BTC503 Thermodynamics and
Biochemical
Engineering 3 - 1 3 - 1 4
BTC504 Bioreactor Analysis and
Technology 3 - 1 3 - 1 4
BTC505 Business
Communication and
Ethics 2 - 2 - - 2 2
BTE501X Elective I 3 - 1 3 - 1 4
BTL501 Bioinformatics Lab - 2 - - 1 - 1
BTL502 Genetic Engineering
Lab 3 1.5 1.5
BTL503 Lab I 3 1.5 1.5
Total 17 12 3 17 6 3 26
Course
code Course Name Examination Scheme
Theory Term
Work Pract/
Oral Oral Total
Internal
Assessment End
Sem
Exam Exam
Duration
(in hrs)
Test 1 Test 2 Avg
BTC501 Bioinformatics 20 20 20 80 3 - - 100
BTC502 Genetic Engineering 20 20 20 80 3 - 100
BTC503 Thermodynamics and
Biochemical Engineering 20 20 20 80 3 25 - - 125
BTC504 Bioreactor Analysis and
Technology 20 20 20 80 3 25 - - 125
BTC505 Business
Communication and
Ethics - - - - - 50 - 50
BTE501X Department Elective I 20 20 20 80 3 25 - - 125
BTL501 Bioinformatics Lab 2 25 25
BTL502 Genetic Engineering Lab 3 25 25
BTL503 Lab I - - - - 3 25 - 25
Total 100 400 - 125 75 - 700
Department Elective I (Sem V)
Engineering Stream Advanced Science Stream Technology Stream
1. Biosensors and Diagnosis
(BTE5011) 1.Biophysics (BTE5012)
2.Biostatistics (BTE5013) 1. Pharmaceutical Technology
(BTE5014)
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University of Mumbai B. E. (Biotechnology) Rev 2016 Page 50
Course Code Course/Subject Name Credits
BTC501 Bioinformatics 4
Pre-requisites:
Basic knowledge of computers, Biochemistry: Structures of DNA, RNA &
Proteins.
Course Objectives:
To develop skills of the Students in the area of Bioinformatics particularly to
make them to learn all the techniques used with biological data.
To study various databases of DNA & Proteins along with current
bioinformatics concepts & their implementation.
To help students ea sily handle proteins by studying in detail about protein
structure.
To become knowledgeable about the storage, retrieval, sharing and use of
biological data, information, and tools.
Course outcomes:
By learning this course the students will be able to :
Cast a molecular biology problem as a bioinformatics problem.
Select relevant tools, optimize their settings and build pipelines to solve the
set problem.
Easily extract the required data from a given set of data & similarly be able to
store it.
Use c onventional softwares and web -based applications.
Analyze processed data with the support of analytical and visualization tools.
Module Contents Contact
Hours
01 Introduction to bioinformatics: Types of biological data,
Sequencing Methods: DNA (Maxim Gilbert Method, Sangers
Method) & Protein (MS-MSAnalysis), Genomic Sequencing,
ESTs and SNPs, Applications of bioinformatics. 06
02 Types of databases: Based on storage techniques( Flat,
Relational, Object Oriented); Based on data (Primary,
Secondary , Sp ecialized)
Search engines: Entrez& SRS Sequence databases: NCBI,
EMBL, DDBJ Structural Databases: PDB Protein Databases:
PIR, SWISS PROT Other Databases: KEGG, TrEMBL, EBI. 08
03 Alignment: Global Alignment, Local Alignment Pair Wise
Sequence Alignment: Dot Matrix Alignment. Dynamic
programming Methods: Needleman Wunch Algorithm, Smith
Waterman Algorithm. Heuristic Method: BLAST, FASTA
Amino Acid Substitution Matrices: PAM, BLOSSUM
Multiple Alignment: CLUSTAL WPhylogenetic Analysis 10
04 Visualization: Methods for representing biological data,
Rasmol, Swiss PDB, 3D Structure Viewers. 04
05 Proteins: Structure, Classification, Classification databases.
Protein Structure prediction: Primary Structure Prediction, 07
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University of Mumbai B. E. (Biotechnology) Rev 2016 Page 51
Secondary Structure Prediction, Tertiary Stru cture Prediction,
Homology Modelling, Chao -Fasman Algorithm, Neural
Networks, Ab -Initio Modelling, Fold recognition (Threading)
06 Interactions: Protein ligand interactions, Torsionangle,
Ramchandran plot, Protein folding & Chaperones. Cartesian
coordinates 10
Assessment
Internal
Assessment consists of two tests which should be conducted at proper
intervals.
End Semester theory examination
Question paper will comprise of 6 questions each carrying 20 questions.
Total 4 questions need to be solved
Question no.1 will be compulsory based on entire syllabus wherein sub
questions can be asked.
Remaining questions will be randomly selected from all the modules
Weightage of marks should be proportional to number of hou rs assigned to
each module
References
1. Oreilly , Developing bioinformatics computer skills, Shroff publishers, 1st
Indian edition
2. David mount, Bioinformatics sequence and genome analysis, CBS
publishers, 2nd edition
3. N. Gautam , Bioinformatics databases & a lgorithm, Narosa publication
4. S. Ignacimuthu S.J, Basic bioinformatics, Narosa publications
5. T. K. Attwood, Introduction to bioinformatics, Pearson education, 8th reprint
6. S. C. Rastogi, Bioinformatics concepts, skills & applications, CBS
publishers, 1st e dition
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University of Mumbai B. E. (Biotechnology) Rev 2016 Page 52
Course Code Course/Subject Name Credits
BTC502 Genetic Engineering 4
Pre-requisites:
Knowledge of Biochemistry, Microbiology, Molecular Biology, Genetics.
Course Objectives:
Give insight into the functioning of Recombinant DNA molecules, their
constructions, analysis and fine tuning.
To engineer such molecules for making of difficult bio -molecules.
This course also gives various ideas and approaches by different schools of
thoughts.
Course outcomes:
By learning this course the students will be able to:
Understand how recombinant molecules are created analysed with respect to
DNA, RNA, and Protein.
They also will be familiar with the problems they could encounter and how
to trouble shoot them.
They will be able to monitor b oth in -vitro and in -vivo activity.
They will be able to suggest more rational approach to solve problem of a
living system at a molecular level.
Module Contents Contact
Hours
01 Basics of genetic material:
DNA structure, topology, Super helical and relaxed
molecules.
Plasmids - Basic features classification, size and copy number,
conjugation & compatibility.
Total cell DNA preparation, different methods of plasmid
DNA preparation. 06
02 Enzymes for Recombinant Technology:
Cutting enzymes - Restriction Endonucleases (Classification,
blunt end, sticky end, mode of action).Introduction to other
enzymes -DNA polymerases, Reverse Transcriptase,
Polynucleotide Kinase, Terminal Transferase, Alkaline
Phosphatase,S1 -Nuclease, Bal -31,
DNA Ligase. 07
03 Cloning vectors:
Vectors used for Gene -cloning: Plasmids (e.g pUC type,
conjugative, Ti etc.), Phages (Lambda and M13 type),
Cosmids and Phagemids. 07
04 Library construction and recombinant gene expression:
Library construction (Genomic and C -DNA typ e) and
Screening for the clone. Foreign gene expression in E. coli,
Fusion proteins 06
05 DNA transfer in to cells: Transformation and Transfection,
Membrane Fusion and Electroporation,
Gene -Gun and Micro -injection 05
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University of Mumbai B. E. (Biotechnology) Rev 2016 Page 53
06 DNA and Protein Analysis:
DNA: Southern and Northern Hybridization. PCR
Amplification, DNA Sequence Analysis (e.g Sangers
Method), Automated Sequencing, RFLP and RAPD. Protein:
Western Blotting, ELISA and its variations 08
07 Antisense and RNA interference Technology and their
applicati ons.
R-DNA in medicine, e.g. Insulin and Blood clotting factor
VIII. 06
Assessment
Internal
Assessment consists of two tests which should be conducted at proper
intervals.
End Semester theory examination
Question paper will comprise of 6 questions each carrying 20 questions.
Total 4 questions need to be solved
Question no.1 will be compulsory based on entire syllabus wherein sub
questions can be asked.
Remaining questions will be randomly selected from all the modules
Weightage of marks should be propor tional to number of hours assigned to
each module
References
1. Molecular Biology of the Cell: Alberts et al.5th. Ed. Garland Publications.
2. Genes VIII: Benjamin Levine, Oxford University Press.
3. Principle of Gene Manipulations (2004): S.B. Primrose, R.M. Twyman & R.
W. Old. Sixth edition. Blackwell Science.
4. Gene Cloning and DNA analysis: An Introduction.(2006).T. A. Brown.
Blackwell Publishing.
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University of Mumbai B. E. (Biotechnology) Rev 2016 Page 54
Course Code Course/Subject Name Credits
BTC503 Thermodynamics and Biochemical Engineering 4
Pre-requisites:
Knowledge of phase rule, knowledge of differentiation & Integration
Course Objectives:
To study the basic concepts of the energy flow in and out of the system.
To apply the thermodynamic principles to the biochemical reactions.
To check the feasibility of the reaction.
Course outcomes:
The student will be able to check the feasibility of a reaction.
Module Contents Contact
Hours
01 Basic Concepts:
System, Surrounding & Processes, Closed and Open systems,
State and Properties, Intensive &Extensive Properties, State
and Path functions, Equilibrium state and Phase rule, Zeroth
law of Thermodynamics, Heat reservoir and Heat engines,
Reversible and Irreversible processes. 02
02 Laws of Thermodynamics:
General statement of First law of Thermodynamics, First
law for Cyclic Process, Non -Flow Process, Flow process,
Heat capacity.
General statements of the second law, Concept of entropy,
The Carnot principle, Calculation of entropy changes,
Clausius inequality, Entropy and Irreve rsibility.
Third law of Thermodynamics. 05
03 PVT Behaviour:
PVT behaviour of pure fluids, equations of state and
ideal gas law, Processes involving ideal gas law: Constant
volume, constant pressure, constant temperature, adiabatic
and polytrophic processes.
Equations of state for real gases: Van -der Waals equation,
Redlich - Kwong equation, Peng -Robinson equation, virial
equation. 04
04 Biochemical Energetics:
Coupled reactions and energy rich compounds, Reaction
Stoichiometry, criteri a of biochemical reaction equilibrium,
equilibrium constant and standard free energy change, effect
of temperature, pressure onfree energy change, effect of
temperature, pressure on equilibrium constants and other
factors affecting equilibrium conversion. Le Chateliers
principle, liquid phase reactions, heterogeneous bioreaction
equilibria, phase rule for reacting systems. 05
05 Properties of Pure Fluids:
Principles of corresponding states, Generalized
compressibility charts. Reference properties, energy 06
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University of Mumbai B. E. (Biotechnology) Rev 2016 Page 55
properties, Derived properties, Helmholtz free energy, Gibbs
free energy, Relationships among thermodynamic Properties:
Exact differential equations, fundamental property relations,
Maxwell’s equations, Clapeyron equations, Entropy heat
capacity relations, modified equations for internal energy
(U)&enthalpy(H),Effect of temperature on U, H &
Entropy(S), Relationships between Cp & Cv, Gibbs -
Helmholtz equation.
06 Fugacity and Activity:
Fugacity: Fugacity, Fugacity coefficient , effect of temperature
and pressure on fugacity, Determination of fugacity of pure
gases, Fugacities of solids and liquids, Activity: Effect of
temperature and pressure on activity. Departure functions and
generalized charts, thermodynamic diagram types of diagrams
and construction of thermodynamic diagrams. 04
07 Properties of Solutions:
Partial molar properties - Partial molar properties of solutions,
determination of partial molar properties, chemical potential
effect of temperature and pressure, Lewis randall rule,
Raoults law for ideal solutions, Henry’s law and dilute
solutions ideal behavior of real solutions and Henrys law,
Activity in solutions, Activity coefficients effect of
temperature and pressure, Gibbs - Duhem equation, Property
changes of mixing, excess properties, excess Gibbs free
energy. 06
08 Phase Equilibria:
Criteria of phase Equilibria, criterion of stability, Duhem’s
theorem, Vapour -Liquid Equilibria, VLE in ideal solutions,
Non-Ideal solutions -azeotropes, VLE at low pressures
activity coefficient equation, bubble point and dew point
equilibria, Consistency test for VLE data using slope of
lncurves, using partial pressure data, calculation of activity
coefficients using Gibbs -Duhem equation, Liquid -Liquid
Equilibrium diagrams binary liquid Equilibrium diagram s. 04
Term Work
Term work shall consist of minimum eight tutorials from entire syllabus which
are to be given at regular intervals Batch wise.
Tutorials: 20 Marks
Attendance: 05 Marks
Total: 25 Marks
Assessment
Internal
Assessment consists of two tests which should be conducted at proper
intervals.
End Semester theory examination
Question paper will comprise of 6 questions each carrying 20 questions.
Total 4 questions need to be solved
Question no.1 will be compulsory based on entire syllab us wherein sub
Page 56
University of Mumbai B. E. (Biotechnology) Rev 2016 Page 56
questions can be asked.
Remaining questions will be randomly selected from all the modules
Weightage of marks should be proportional to number of hours assigned to
each module
References
1. Smith, J.M., Van Ness, H.C., and Abbott, M.M. Introduction to Chemical
Engineering Thermodynamics, McGraw Hill, Inc..
2. Kyle, B.G., Chemical and Process Thermodynamics, Prentice Hall, Inc.
3. Hougen, O.A., Watson, K.M., and Ragatz, R.A., Chemical Process
Principles Part II, John Wiley & Sons, (CBS Publish ers & Distributors, New
Delhi).
4. YVC Rao, "An Introduction to Thermodynamics", University Press, Second
Edition.
5. K V Narayanan, "Textbook of Chemical Engineering Thermodynamics",
PHI Learning Second Edition.
Page 57
University of Mumbai B. E. (Biotechnology) Rev 2016 Page 57
Course Code Course/Subject Name Credits
BTC504 Bioreactor Analysis and Technology 04
Pre-requisites:
Knowledge of chemical reaction kinetics, Knowledge of differentiation and
integration
Course Objectives:
To understand the basic concepts of Bioreactor design.
To select the relevant principles and data for practical process engineering
purposes.
Course outcomes:
Student will be able to understand the di fferent types of ideal and non - ideal
reactors.
Student will be able to design the reactors required for a particular
processes.
Module Contents Contact
Hours
01 Basic Reaction Kinetics:
Reaction thermodynamics, order and molecularity of reaction,
homogeneous and heterogeneous reactions, elementary and
non-elementary reactions, reaction yield, reaction rate,
calculation of reaction rates from experimental data, general
reaction kinetics for biological system, production kinetics in
cell culture, kinetics of substrate uptake in cell culture, growth
kinetics with plasmid instability 06
02 Ideal Reactors:
Constant volume and variable reactors, batch operation of a
well-mixed enzyme and cell culture reactor, fed batch
operation ofa well -mixed enzyme and cell culture reactor,
continuous operation of well mixed enzyme and cell culture
reactor, continuous oper ation of plug flow enzyme and cell
culture reactor, autocatalytic reactions, recycle reactors -plug
flow reactor and continuous stirred tank reactor, comparison
between major modes of reactor operation. 07
03 Multiple Reactors and Reaction Systems:
Continuou s stirred tank reactors of equal size in series,
continuous stirred tank reactors of unequal size in series,
finding conversion in given system, determining the best
system for a given conversion, plug flow reactors in series and
parallel, reactors of different types in series. Simple reactions,
step wise reactions, parallel reactions, series reactions,
maximizing R in batch reactor, plug flow reactor and
continuous stirred tank reactor, reactor choice for series
reactions and series parallel reactions, concepts of reversible
reactions. 07
04 Heterogeneous Reactions: 06
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University of Mumbai B. E. (Biotechnology) Rev 2016 Page 58
Heterogeneous reactions in Bioprocessing, Concentration
gradients and reaction rates in solid catalysts, Internal mass
transfer and reactions, steady state mass balance (spherical
geometry), Concentration profile for first order kinetics,
Concentration profile for zero order kinetics, Concentration
profile for Michaelis - Menten kinetics, Effectiveness factor
and Thiele Modulus, External mass transfer
05 Deviations from ideal reactors:
Concep t of nonideality, reasons of non ideality, RTD studies,
F curve, C curve, E curve, diagnosis of ills of flow reactors,
modelling of nonideal behaviour -dispersion model, tanks in
series model. 05
06 Working principle o f unconventional reactors:
Selection criterion for bioreactors, Bubble column, Airlift
reactor, Fluidizedbed reactor, perfusion reactors, membrane
reactors 04
Term Work
Term work shall consist of minimum eight tutorials from entire syllabus which
are to be given at regular intervals Batch wise.
Tutorials: 20 Marks
Attendance: 05 Marks
Total: 25 Marks
Assessment
Internal
Assessment consists of two tests which should be conducted at proper
intervals.
End Semester theory examination
Question pa per will comprise of 6 questions each carrying 20 questions.
Total 4 questions need to be solved
Question no.1 will be compulsory based on entire syllabus wherein sub
questions can be asked.
Remaining questions will be randomly selected from all the modules
Weightage of marks should be proportional to number of hours assigned to
each module
References
1. D.G. Rao, Introduction to Biochemical Engg, Tata McGraw Hill Edu. Pvt.
Ltd., Second edition.
2. P.M. Doran, Bioprocesses Engg. Principles, Academic Press, London,
Second edition.
3. E.H. Perry 7 D.W. Green, Perrys Chemical Engineering Handbook, Seventh
edition.
4. Octave Levenspiel, Chemical Reaction Engineering, John Wiley Publication,
Third Edn
5. H Scott Fogler "Elements of Chemical Reaction engineering" Prentic e Hall,
2006
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University of Mumbai B. E. (Biotechnology) Rev 2016 Page 59
Course Code Course/Subject Name Credits
BTC505 Business Communication and Ethics 2
Prerequisites:
Students should have basic knowledge of English and general engineering.
Course Objectives
To inculcate in students professional and ethical attitude, effective
communication skills, teamwork, multidisciplinary approach, and an ability to
understand Engineers’ social responsibilities
To provide students with an academic environment where they will be aware
of the excellence, leadership and lifelong learning needed for a successful
professional career
To inculcate professional ethics and codes of professional practice
To prepare students for successful careers that meets the global Industrial and
Corporate requirement
Course Outcomes:
Students will be able to
Communicate effectively in both oral and written form and equip to
demonstrate knowledge of professional and ethical responsibilities.
participate and succeed in campus placements and competitive examinations
like GATE, TOFEL
Posses s entrepreneurial approach and ability for life -long learning
Have education necessary for understanding the impact of engineering
solutions on Society, and demonstrate awareness of contemporary issues
Detailed Syllabus.
Design a technical document using precise language, suitable vocabulary and
apt style.
Develop the life skills/ interpersonal skills to progress professionally by
building stronger relationships.
Demonstrate awareness of contemporary issues knowledge of professional and
ethical responsibil ities.
Apply the traits of a suitable candidate for a job/higher education, upon being
trained in the techniques of holding a group discussion, facing interviews and
writing resume/SOP.
Deliver formal presentations effectively implementing the verbal and non-
verbal skills.
Module Contents Contact
Hours
1 Report Writing
Objectives of Report Writing
Language and Style in a report
Types : Informative and Interpretative (Analytical, Survey and
Feasibility) and Formats of reports (Memo, Letter, Short and
Long Report) 05
2 Technical Writing
Technical Paper Writing (IEEE Format)
Proposal Writing 03
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University of Mumbai B. E. (Biotechnology) Rev 2016 Page 60
3 Introduction to Interpersonal Skills
Emotional Intelligence
Leadership and Motivation
Team Building
Assertiveness
Conflict Resolution and Negotiation Skills
Time Management
Decision Making 09
4 Meetings and Documentation
Strategies for conducting effective meetings
Notice, Agenda and Minutes of a meeting
Business meeting etiquettes 02
5 Introduction to Corporate Ethics
Professional and work ethics (responsible use of social media -
Facebook, WA, Twitter etc.)
Introduction to Intellectual Property Rights
Ethical codes of conduct in business and corporate
activities(Personal ethics, conflicting values, choosing a moral
response and
making ethical decisions) 02
6 Employment Skills
Group Discussion
Resume Writing
Interview Skills
Presentation Skills
Statement of Purpose 07
Term Work
The term work shall be comprised of the neatly written Journal comprising below
mentioned assignments.
Assignment 1 - Interpersonal Skills (Group activity Role play)
Assignment 2 - Interpersonal Skills (Documentation in the form of soft copy or
hard copy)
Assignment 3 - Cover Letter Resume
Assignment 4 - Report Writing
Assignment 5 - Technical Proposal (document of the proposal)
Assignmen t 6- Technical Paper Writing
Assignment7 -Meetings Documentation (Notice, Agenda, Minutes of Mock
Meetings)
Assignment 6 - Corporate Ethics (Case study, Role play)
Assignment 8 - Printout of the PowerPoint presentation
Term -work Marks: 50 Marks
The marks of term -work shall be judiciously awarded depending upon the quality
of the term work including that of the report on experiments assignments. The final
certification acceptance of Term work warrants the satisfactory the appropriate
completion of the assig nments, presentation, book report, group discussion and
internal oral the minimum passing marks to be obtained by the students. The
following weightage of marks shall be given for different components of the term
work .
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University of Mumbai B. E. (Biotechnology) Rev 2016 Page 61
Attendance : 05 Marks
Assignments : 2 0 Marks
Internal Oral: 25 Marks comprising of:
Presentation of the Project Report: 10 Marks
Book Report (one copy per group): 05 Marks
Group discussion: 10 Marks
References
1. Fred Luthans, “Organizational Behavior”, McGraw Hill, edition
2. Lesiker and P etit, “Report Writing for Business”, McGraw Hill, edition
3. Huckin and Olsen, “Technical Writing and Professional Communication”,
McGraw Hill
4. Wallace and Masters, “Personal Development for Life and Work”, Thomson
Learning, 12th edition
5. Heta Murph y, “Effective Business Communication”, McGraw Hill, edition
6. Sharma R.C. and Krishna Mohan, “Business Correspondence and Report
Writing”, Tata McGraw -Hill Education
7. Ghosh,B . N.,“Managing Soft Skills for Personality Development”, Tata
McGraw Hill. Lehman,
8. Dufrene, Sinha, “BCOM”, Cengage Learning, 2ndedition
9. Bell, Smith, “Management Communication” Wiley India Edition,3rdedition.
10. Dr. Alex, K.,”Soft Skills”, S Chand and Company
11Subramaniam,R., “Professional Ethics” Oxford University Press.
12. Robbins Stephens P., “Organizational Behavior”, Pearson Education
13. https://grad.ucla.edu/asis/agep/advsopstem.pdf
Page 62
University of Mumbai B. E. (Biotechnology) Rev 2016 Page 62
Course Code Course/Subject Name Credits
BTE5011 Department Elective I – Biosensors and Diagnostics 4
Pre-requisites:
Biochemistry, Analytical methods in Biotechnology, Principles of basic
instruments used in a Biotechnology laboratory.
Course Objectives:
The objectives of this course is that the students will be able to:
Explain the role of biological macromolecules as recognition elements &
biosensors.
Describe the biomedical aspects of these sensors.
Analyse the interplay between materials, components and systems in the field
of bio sensing.
Design an advanced biosensor for medical applications, using the current
state of the art of biosensors.
Describe what challenges are sha red among and what challenges are unique
to the major biosensor application areas.
Course outcomes:
By learning this course the students will be able to :
Apply the principles of engineering to the development ofbioanalytical
devices and the design of biosensors
Explain the principles of linking cell components and biological pathways
with energy transduction, sensing and detection.
Differentiate among various biosensor systems.
Design a biosensor in response to agricultural, bioenvironmental, food saf ety,
and biosecurity applications.
Apply engineering and biological approaches to solve problems in diagnosis
of diseases, such as diabetes, cancer or detection of other analytes/
biomarkers.
Module Contents Contact
Hours
01 Biosensors: Principles, Characteristics of Ideal Biosensors,
Basic measuring procedure, Components of biosensors,
Advantages & Limitations 08
02 Bio catalysis based biosensors, Bioaffnity based biosensors
&Microorganisms based biosensors, Biologically active
material and analyte. Types of membranes used in biosensor
constructions. 09
03 Various types of transducers, Principles and applications
Calorimetric, Optical, Potentiometric/ Amperometric,
Conductrometric/ resistormetric. 05
04 Piezoelectr ic, Semiconductor, Impedimetric, Mechanical and
molecular electronics based transducers, Chemiluminiscene -
based biosensors. 05
05 Biosensors in clinical chemistry, Medicine and health care,
Biosensors for veterinary, Agriculture and food, Low cost
biosens or for industrial processes for online monitoring, 08
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University of Mumbai B. E. (Biotechnology) Rev 2016 Page 63
Biosensors for environmental monitoring.
Term Work
Term work shall consist of minimum eight tutorials from entire syllabus which
are to be given at regular intervals Batch wise.
Tutorials: 20 Marks
Attendance: 05 Marks
Total: 25 Marks
Assessment
Internal
Assessment consists of two tests which should be conducted at proper
intervals.
End Semester theory examination
Question paper will comprise of 6 questions each carrying 20 questions.
Total 4 questions need to be solved
Question no.1 will be compulsory based on entire syllabus wherein sub
questions can be asked.
Remaining questions will be randomly selected from all the modules
Weightage of marks should be proportional to number of hou rs assigned to
each module
References:
1. Roger, K.R. and Gerlach, C.L. 199. Update on environmental for biosensors.
Env. Sci. Techno! 33 500A - 506A.
2. Bilitewski, U. Turner, A.P.F. 2000 Biosensors for environmental monitoring
Harwood, Amsterdam.
3. Moses, V and Cape, R.E. 1991, Biotechnology the science and business,
Harwood, Academic Publisher London
4. Rogers, K.R. and Mascini, M. 2001. Biosensors for analytical monitoring
EPA biosensors group.
5. Aboul - Enein, H. V., Stefan, R. and Van Staden, (1999) Chemiluminiscence
- based biosensors - An overview crit Rev. Anal. Chem. 29, 323 -331.
6. Pearson, J.E. Gill, A., and Vadgama, P. (2000) Analytical aspects of
biosensors, Ann Clin Biochem 37, 119 -145.
7. Biosensors: Fundamentals and applications, Oxford, U.K: O xford University
Press by Turner, A.P.F., Karube, I. & Wilson, GS.
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University of Mumbai B. E. (Biotechnology) Rev 2016 Page 64
Course Code Course/Subject Name Credits
BTE5012 Department Elective I – Biophysics 4
Pre-requisites:
Knowledge of Chemistry, Physics, Atomic physics, Biochemistry,
Molecular Biology.
Course Objectives:
The objective of this course is to give insight into the structure of various
macro -molecules, their constructions, analysis and interactions.
Course outcomes:
By learning this course the students will be able to:
Understand how molecules are created and studied.
They will be able to monitor both in -vitro and in -vivo activity and
interactions.
Module Contents Contact
Hours
01 Introduction to Biophysical Techniques:
Electron Microscopy, Atomic Force Microscopy, X ray
Crystallography, NMR Spectroscopy, Infrared Spectroscopy,
Circular Dichroism 10
02 Nucleic Acid interactions:
Carrier RNA, Interactions between DNA & protein, Zinc
finger proteins, various nucleic acid binding proteins, Nuclear
transport. 06
03 Membrane Structure & Properties:
The principles governing the structures of biological
membrane, Two -dimensional fluids, Assembly of membrane
components. 06
04 Protein Structural study:
Intra and inter -molecular forces, helix -coil transitions and
protein folding in a thermodynamical context, Secondary
Motifs, Tertiary Architecture and Quaternary Organization,
crystallization, diffraction theory. 07
05 Lipids & their Interaction:
Details of Lipid Structures, Lipoproteins and Glycolipids.
High density (HDL) and low density (LDL) lipoprotein.
Disorder caused by saturated fat and cholesterol,
Arteriosclerosis. 06
Term Work
Term work shall consist of minimum ei ght tutorials from entire syllabus which
are to be given at regular intervals Batch wise.
Tutorials: 20 Marks
Attendance: 05 Marks
Total: 25 Marks
Assessment
Internal
Page 65
University of Mumbai B. E. (Biotechnology) Rev 2016 Page 65
Assessment consists of two tests which should be conducted at proper
intervals.
End Semester theory examination
Question paper will comprise of 6 questions each carrying 20 questions.
Total 4 questions need to be solved
Question no.1 will be compulsory based on entire syllabus wherein sub
questions can be asked.
Remaining questions will be randomly selected from all the modules
Weightage of marks should be proportional to number of hours assigned to
each module
References
1. Cantor R, Samual P. R. (1985). Biophysical Chemistry. W. H. Freeman &
Co.
2. Vasantha Pattabhi, N. Gautham. Biophysics. Narosa Publishing House Ltd.
3. Van Holde Johnson and Ho. (2006) Principles of Physical Biochemistry.
Second Edition, Pearson Prentice Hall.
4. Igor N. Serdyuk, Nathan R. Zaccai, & Joseph Zac. Methods in Molecular
Biophysics: Structure, dyna mics and Function. Cambridge University Press.
5. Physical Biochemistry: Principles and applications by David Sheehan, Jon
Wiley & Sons.
Page 66
University of Mumbai B. E. (Biotechnology) Rev 2016 Page 66
Course Code Course/Subject Name Credits
BTE5013 Department Elective I – Biostatistics 4
Pre-requisites:
Knowledge of basic statistical methods
Course Objectives:
To understand statistics - basic theory, philosophy and application of
statistics
To Understand why biologists need a background in statistics in conducting
research
To help students know the role of statistics as a tool for scientists
Course outcomes:
By learning this course the students will be able to:
Identify and implement statistical techniques and models for analysis of
biological data.
To enable them interpret research.
Module Contents Contact
Hours
01 Descriptive statistics and Probability: Frequency distribution,
Measures of Central tendancy, Measures of Dispersion, Basic
probability and Bayes theorem. 03
02 Probability and sampling distributions; Discrete probability
distributions; Continuous probability distributions - Binomial,
Poisson and normal distributions; Sampling Distributions -
sample mean, difference between two sample means, Sample
proportions, difference between two sample proportions. 08
03 Estimation; t -distribution; Confidence intervals for -
population mean, difference between two population means,
population proportion, difference between two population
proportions, Variance of normally distributed population,
ratio of variances of two nor mally distributed populations.
Determination of sample size for estimating mean and
proportions. 05
04 Hypothesis Testing: Hypothesis testing for - population mean,
difference between two population means, population
proportion, difference between two pop ulation proportions,
population variance, ratio of two population variances,
Type I and II error and Power of test 05
05 Analysis of Variance: Completely randomized design,
Randomized complete block design, repeated measures
design, factorial experiment. Regression and Correlation:
Simple linear regression, correlation model, correlation
coefficient, multiple regression, multiple correlation 09
06 Chi square distribution and analysis of frequency, Chi-square
distribution properties, Test of goodness of fi t, independence
and homogeneity 05
Page 67
University of Mumbai B. E. (Biotechnology) Rev 2016 Page 67
List of Tutorials:
Descriptive statistics and probability
Discrete probability distributions
Continuous probability distributions
Sampling distributions
Estimation
Hypothesis testing
Analysis of Variance
Regression and correlation
Chi square distribution and analysis of frequency
Term Work
Term work shall consist of minimum eight tutorials from entire syllabus which
are to be given at regular intervals Batch wise.
Tutorials: 20 Marks
Attendance: 05 Marks
Total: 25 Marks
Assessment
Internal
Assessment consists of two tests which should be conducted at proper
intervals.
End Semester theory examination
Question paper will comprise of 6 questions each carrying 20 questions.
Total 4 questions need to be solved
Question no.1 will be compulsory based on entire syllabus wherein sub
questions can be asked.
Remaining questions will be randomly selected from all the modules
Weightage of marks should be proportional to number of hours assigned to
each module
Textbooks
1. Biostatistics - A foundation for analysis in health sciences by Wayne W.
Daniel, Seventh edition, Wiley India
2. Fundamentals of Mathematical statistics by S.C.Gupta and V.K. Kapoor,
second edition, Sultan Chand Publishers
3. Probability and statistics for engin eers by J. Ravichandran, Wiley India
4. Biostatistics - How it works by Steve Selvin, Pearson Education
5. An introduction to Biostatistics by Sunder Rao and J. Richard, third edition,
Prentice Hall of India
6. Probability and Statistics by Schaum's series
Page 68
University of Mumbai B. E. (Biotechnology) Rev 2016 Page 68
Course Code Course/Subject Name Credits
BTE5014 Department Elective I – Pharmaceutical Technology 4
Pre-requisites:
Knowledge about biochemistry and biochemical pathways in biological
systems.
Knowledge about cell biology and metabolism
Course Objectives:
To understand the basic aspects of pharmacokinetics and
pharmacodynamics.
To give an insight about the pre -clinical and clinical trials and different
classes of drugs.
Course outcomes:
Students will be able to tell factors affecting the bioavailability and
stability of dosage form. They also know the parameters for the
disposition, absorption and Michaelis -Menton constants for non -linear
kinetics.
Students will know the fabrication, design, evaluation and application
of drug delivery systems.
Module Contents Contact
Hours
01 Introduction To Pharmaceuticals: History & Definition of
Drugs. Sources of Drugs - Plant, Animals, Microbes and
Minerals. Drug targets, Intermolecular bonding forces.
Classification of Drugs. Naming of Drugs and medicines 04
02 Pharmacodynamics and Pharmaco kinetics: Molecules acting
as drug targets: Enzymes, Receptors, Nucleic acid Drug
Absorption, Distribution, Metabolism and Excretion
(ADME). Modes of drug administration Drug dosing (half -
life, steady state concentration, drug tolerance,
Bioavailability). Drug delivery system 08
03 Finalstages of drug development -trials: Preclinical and
clinical trials, Patenting and regulatory affairs 04
04 Medicinal Chemistry: Antibacterial, Anti cancer, Antiviral
drugs, Opioid analgesics 12
05 Biopharmaceuticals: Production of Therapeutic Proteins,
Hormones, Nucleic acids, Role of Biopharmaceuticals in
treatment of various health disorders 06
Term Work
Term work shall consist of minimum eight tutorials from entire syllabus which
are to be given at regular intervals Batch wise.
Tutorials: 20 Marks
Attendance: 05 Marks
Total: 25 Marks
Assessment
Internal
Page 69
University of Mumbai B. E. (Biotechnology) Rev 2016 Page 69
Assessment consists of two tests which should be conducted at proper
intervals.
End Semester theory examination
Question paper will comprise of 6 questions each carrying 20 questions.
Total 4 questions need to be solved
Question no.1 will be compulsory based on entire syllabus wherein sub
questions can be asked.
Remaining questions will be randomly selected from all the modules
Weightage of marks should be proportional to number of hours assigned to
each module
References
1. Biopharmaceuticals: Biochemistry & Biotechnology, Gary Walsh (1998),
John Wiley &Sons Ltd.
2. Medicinal Chemistry by Graham L. Patrick, Oxford University Press
3. Remingtons Pharmaceutical sciences, (Mark Publications & Company eston
PA) 1980 .
4. Medicinal Chemistry: an introduction by Gareth Thomas, Wiley
Publications
5. Theory & Practice of Industrial Pharmacy, (3rd ed.)Leon Lachman, Lea &
Febiger (1986)
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University of Mumbai B. E. (Biotechnology) Rev 2016 Page 70
Course Code Course/Subject Name Credits
BTL501 Bioinformatics Lab 1
Concept of Experiments:
Ten experiments must be performed
Access & use of different databases using NCBI metadatabase.
To study use of ORF finder to find the correct reading frame.
To study the characteristics of protein using PROT SCALE.
To study prediction of coding sequence (CDS) of a gene using NCBI
& Genemark and compare the results for percentage accuracy.
To access & use different online gene & protein alignment softwares.
Protein structure visualization using ’RASMOL’ graphical user interface.
Protein structure visualization using ’RASMOL’ command line inte rface.
Secondary structure prediction for amino acid sequences of a given protein.
Homology modelling of protein using SWISS -PDB modeller.
To study chemical structure of drugs using Chemsketch & Marvinsketch.
To find & study phylogenetic relationships among different given species
using CLUSTAL OMEGA.
To study multiple sequence alignment (MSA) tools & compare the results.
To study BLOCKS using Interpro.
To study EXPASY tool for protein structure analysis.
To find and study gene using MAP -VIEWER.
Practical Examination
Duration for practical examination would be the same as assigned to the
respective lab per week.
A student becomes eligible for practical examination after completing a
minimum of eight experiments out of ten experiments
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University of Mumbai B. E. (Biotechnology) Rev 2016 Page 71
Course Code Course/Subject Name Credits
BTL502 Genetic Engineering Lab 1.5
Concept of Experiments:
Ten experiments must be performed
Isolation of Genomic DNA
Purification and quantification of isolated DNA.
Separation of DNA by Agarose gel electrophoresis.
PCR analysis of DNA fragments by agarose gel electrophoresis
Making the bacterial cells competent
Transformation of E.coli.
Isolation of plasmid DNA by boiling lysis method.
Isolation plasmid DNA by Alkaline lysis method.
Southern blotti ng
Southern Hybridization
In vitro DNA ligation
RFLP technique
Protein Analysis by SDS -PAGE
Bacterial conjugation
Bacterial survival against UV irradiation and mutagenesis
Isolation of mutants, e.g.auxotrophs, by chemical mutagenesis.
(Acridineorange/Ethydium bromide)
β-galactosidase activity of lac+ & lac - mutant of E.coli.
Primary screening of antibiotic producers from soil
Practical Examination
Duration for practical examination would be the same as assigned to the
respective lab p er week.
A student becomes eligible for practical examination after completing a
minimum of eight experiments out of ten experiments
Page 72
University of Mumbai B. E. (Biotechnology) Rev 2016 Page 72
Course Code Course/Subject Name Credits
BTL503 Lab I 1.5
Concept of Experiments:
Ten experiments must be performed
Physical method of microbial control.
Chemical method of microbial control.
Isolation of bioluminescent organisms.
Diauxic growth curve of E.Coli.
Biochemical characterization of microbes (IMVIC test, Catalase, Oxidase
test).
Detection o f Amino acid producer from soil.
Acid fast staining for mycobacteria.
Study of air microflora& determination of sedimentation rate.
Blood film preparation and identification of cells.
Antibiotic susceptibility test.
E Test
Blood group typing using haemag glutination tests.
To detect the antigen/antibody using Enzyme Linked Immuno Sorbent Assay
(ELISA)
To test the pattern of antigen -antibody interaction through Ouchterlony
double diffusion assay
RID
Lymphoid organs and their microscopic organization
Separation of mononuclear cells by Ficoll -Hypaque
VDRL test (Demonstration)
Immunodiagnostics (demonstration using commercial kits)
Determination of MIC of antibacterial drugs.
Identification of AgAb complex by Slide agglutination test
Practical Examinat ion
Duration for practical examination would be the same as assigned to the
respective lab per week.
A student becomes eligible for practical examination after completing a
minimum of eight experiments out of ten experiments
Page 73
University of Mumbai B. E. (Biotechnology) Rev 2016 Page 73
University of Mumbai
Program Structure for B.E. Biotechnology (Revised 2016 )
T.E. Semester VI (w.e.f 2018 -2019 )
Course
code Course Name Teaching Scheme
(Contact Hours)
Credits Assigned
Theory Practical Tutorial Theory Practical Tutorial Total
BTC601 Food Technology 3 - 1 3 - 1 4
BTC602 Cell & Tissue Culture 4 - - 4 - - 4
BTC603 Enzyme Engineering 4 - 4 - - 4
BTC604 IPR, Bioethics and Bio
safety 3 - 1 3 - 1 4
BTC605 Process Control &
Instrumentation 3 - 1 3 - 1 4
BTE602X Elective -II 3 - 1 3 - 1 4
BTL601 Lab-II - 3 - - 1.5 - 1.5
BTL602 Lab-III - 3 - - 1.5 - 1.5
Total 20 6 4 20 3 4 27
Course
code Course Name Examination Scheme
Theory Term
Work Pract/
Oral Oral Total
Internal
Assessment End
Sem
Exam Exam
Duratio
n
(in hrs)
Test 1 Test 2 Avg
BTC601 Food Technology 20 20 20 80 3 25 - - 125
BTC602 Cell & Tissue Culture 20 20 20 80 3 - - - 100
BTC603 Enzyme Engineering 20 20 20 80 3 - - - 100
BTC604 IPR, Bioethics and Bio safety 20 20 20 80 3 25 - - 125
BTC605 Process Control &
Instrumentation 20 20 20 80 3 25 - - 125
BTE602X Elective -II 20 20 20 80 3 25 - - 125
BTL601 Lab-II - - - - 3 - 25 25
BTL602 Lab-III - - - - 3 - 25 - 25
Total 120 480 - 100 50 -- 750
Department Elective II (Sem VI)
Engineering Stream Advanced Science Stream Technology Stream
1. Computational Fluid Dynamics
(BTE6021) 1.Protein Engineering
(BTE6022)
2. Cancer Biology (BTE6023) 1. Green technology
(BTE6024)
Page 74
University of Mumbai B. E. (Biotechnology) Rev 2016 Page 74
Course Code Course/Subject Name Credits
BTC601 Food Technology 4
Pre-requisites:
Basic concepts of Microbiology and Fermentation Technology
Course Objectives:
To impart knowledge of various areas related to Food science and
technology
To enable the students to understand food composition and its
physiochemical, nutritional and microbiological aspects
To familiarize the students about the processing and preservation techniques
of Food products
Course outcomes:
Students will know the p rinciples of preservation.
Students will understand the principles of food processing techniques and
will be able to apply these principles to specific food commodities.
Module Contents Contact
Hours
01 Introduction to food technology, Constituents of food,
contribution to texture, flavour and organoleptic properties of
food; food additives coloring agents, emulsifiers,
preservatives, flavours, vitamins, organic acids and their
functions; enzymes in food processing 06
02 Sources and activity of microorgan isms associated with food;
Factors affecting the growth and survival of micro -organisms
in foods -intrinsic and extrinsic; Food borne diseases,
infections and intoxications, food spoil agecauses. Microbial
food- yeasts, bacteria and production of new protei n foods -
SCP, mushroom, algal proteins 07
03 Microbial fermentation and production of food and beverages
using microorganisms. Pickling, Sauerkraut, vinegar, bread.
Dairyproduct -Yogurt, cheese production by microbial and
enzymatic (proteases) method. Alcoholic beverages -Beer
(deoxygenating and desugaring by glucose oxidase of beer,
beer mashing and chill proofing), Wine (red, white, sparkling) 07
04 Fermentation methods for preserving foods, Preparation of
various food additives like coloring agents, emulsifiers,
vitamins, flavours and organic acids 05
05 Post Harvest technology for food crops. Food preservation -
high temperature methods, low temperature methods,
irradiation, high pressure method and chemical preservatives.
Production of Fruit juices and types of Fruit juices 05
06 Food Packaging methods:
Materials used for food packaging of various food products
like cheese, eggs, bread, alcoholic beverages, milk and juices 05
Term Work
Term work shall con sist of minimum eight tutorials from entire syllabus which
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University of Mumbai B. E. (Biotechnology) Rev 2016 Page 75
are to be given at regular intervals Batch wise.
Tutorials: 20 Marks
Attendance: 05 Marks
Total: 25 Marks
Assessment
Internal
Assessment consists of two tests which should be conducted at proper
intervals.
End Semester theory examination
Question paper will comprise of 6 questions each carrying 20 questions.
Total 4 questions need to be solved
Question no.1 will be compulsory based on entire syllabus wherein sub
questions can be asked.
Remaining questions will be randomly selected from all the modules
Weightage of marks should be proportional to number of hours assigned to
each module
References
1. Frazier, Food Microbiology, Tl -IM Publications.
2. Heller, Genetic Engineering of Food: Detection of Genetic Modifications -
Wiley Publictions.
3. Le.A. et.al., Microorganism & Fermentations - N.Y. Chemical
4. Rehm, Biotechnology Set Wiley Publications
5. M. R. Adams and M. O. Moss, Food Microbiology, Royal society of
chemistry
6. James M. Jay, Mo dern food microbiology, An Aspen Publications
7. Prescott and Dunn, Industrial Microbiology, CBS Publications.
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University of Mumbai B. E. (Biotechnology) Rev 2016 Page 76
Course Code Course/Subject Name Credits
BTC602 Cell and Tissue Culture 4
Pre-requisites:
Basic knowledge of Cell Biology, Microbiology and Plant and Animal
Physiology
Course Objectives:
To examine and analyse practical and theoretical principles of cell culture
To explain the conditions under which cells can be cultured outside the body.
To explain the advantages and limit ations of cell culture in biomedical
research and applications.
Course outcomes:
By learning this course the students will be able to:
Plan experiments using cultured cells.
Carry out cell culture, and associated laboratory techniques.
Carry out the most common analysis techniques associated with cell culture.
Perform adequate statistical processing of data generated by cell culture.
Present and analyse literature which covers cell culture.
Module Contents Contact
Hours
01 Plant tissue culture Introduction:
Internal organization of plant, Plant Tissue Culture Media,
Plant growth hormones, Concept of Totipotency, Study of
various types of Organ Culture, Organogenesis,
Micropropagation 08
02 Plant Cell Culture And its Applications:
Plant Cell Suspension Culture, Single Cell Culture, Somatic
Embryogenesis,ArtificialSeeds,ProtoplastCulture&SomaticHy
bridization, Scale -up and Automation of Plant Cell Culture 08
03 Transformation of Plants:
Agrobacterium mediated Gene transfer, Chemical Methods,
Virus Mediated Gene Transfer, Transgenic Plants,
Applications of Plant Biotechnology for Production of
Quality Oil, Industrial Enzymes and Plantibodies 06
04 Introduction to Animal Cell Culture: Advantages and
limitations of Animal Tissue Culture, Laboratory Design &
Layout of ATC laboratory, Equipments and Materials of a
Tissue Culture Laboratory, Media Preparation and
Sterilization techniques, 08
05 Primary and secondary cell Culture:
Establishment and maintenance of primary cell cultures of
adherent and non-adherent cell lines, Establishment and
maintenance of seconary and continuous cell cultures. 05
06 Characterization of cell lines:
Cell morphology, Chromosome preparation and analysis,
karyotyping, chromosome banding, Chromosome painting,
Spectral kar yotyping (SKY). DNA analysis - DNA 05
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University of Mumbai B. E. (Biotechnology) Rev 2016 Page 77
hybridization, DNA fingerprinting
07 Application of cell culture:
Vaccine production, Antibody Engineering and large scale
production of pharmaceutical products, Production of
cytokines and therapeutic recombinant glyc oproteins. 05
Assessment
Internal
Assessment consists of two tests which should be conducted at proper
intervals.
End Semester theory examination
Question paper will comprise of 6 questions each carrying 20 questions.
Total 4 questions need to be solved
Question no.1 will be compulsory based on entire syllabus wherein sub
questions can be asked.
Remaining questions will be randomly selected from all the modules
Weightage of marks should be proportional to number of hours assigned to
each module
Reference s
1. Plant Tissue Culture: Theory and Practice :Theory and Practice By S.S.
Bhojwani, M.K. Razdan; Elsevier Publishers
2. Plant Tissue Culture by Kalyan Kumar De; Published by New Central Book
Agency, 1997
3. Plant Tissue and Cell Culture; Volume11 of Botanical monographs, by
Herbert Edward Street; Publisher: University of California Press, 1973
4. Culture of Animal Cells: A Manual of Basic Technique and Specialized
Applications By R. Ian Freshney; Wiley Publishers
5. Animal Cell Culture ( Introduction to Biotechniques): Sara j.Morgan, David
C. Darling; Published by BIOS Scientific Publishers Ltd., 1993
6. Principles and Practice of Animal Tissue Culture – Sudha Gangal.
7. Basic Cell Culture Vol. 290 Protocols by Cheryl D Helgason, Cindy L
Miller. Humanan Press.2 005.
8. Basic Cell Culture 2nd Edition by JM Davis Oxford University Press.2002.
9. Biotechnology by B. D. Singh: Expanding horizons. Kalyani Publishing.
2008.
10. Animal Cell Culture & Technology by M. Butler, Published by Taylor &
Francis publishers.
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University of Mumbai B. E. (Biotechnology) Rev 2016 Page 78
Course Code Course/Subject Name Credits
BTC603 Enzyme Engineering 4
Pre-requisites:
Knowledge of Biochemistry, Microbiology &Molecular Biology.
Course Objectives:
The objectives of this course are to:
Give insight into the functioning of Enzyme molecules (Biological Catalyst),
their constructions, Structure, interactions with other cellular molecules, and
the process of catalysis.
Students will learn to use such molecules for making of difficult bio-
molecules.
They will also be able to understan d Industrial uses and applications of
Enzymes.
Course outcomes:
By learning this course the students will be able to:
Understand how Enzymes are created as functional bio -catalysts, analysed
with respect to their effciencies, their lability, and ways to make them
durable.
They also will be familiar with the problems they could encounter and how
to trouble shoot them.
They will be able to monitor both in -vitro and in -vivo activity.
Module Contents Contact
Hours
01 Enzymes as Biological Catalysts, Chemical nature &
Structure of Enzymes, Properties of enzymes, Enzyme Units,
Nomenclature & Classification, Catalytic and Allosteric site,
Models of Enzyme -substrate interactions, Activation Energy
and catalysis, Effect of pH, Temperature and Salts on Enzyme
activity 08
02 Enzyme Kinetics: Henri & Michaelis -Menten Equation & its
significance, Briggs Haldane modification, Line weaver -
Burke, Eadie -Hofstee & Hanes plot, Problems.
Enzyme Inhibition: Irreversible & Reversible (Competitive,
Uncompetitive, Noncompetitive, Mixed, Partial), Substrate
Inhibition, Allosteric Inhibition. 11
03 Enzyme Assay: Kinetic determination of catalytic activity,
Coupled kinetic assay, Radioimmunoassay. Instrumental
techniques for Enzymatic Analysis: Manometry,
Spectrophotometry, Spectrofluorimetry, Electrochemical
methods (Potentiometric & Conductometry) Enthalpimetry,
Radiochemical methods, Dry reagent techniques. 06
04 Working with Enzyme: Isolation & Extraction of Enzymes
from various so urces and locations in cell.
Purification of Enzymes: Preliminary purification, further
purification using chromatography & electrophoresis Criteria
of Purity: Specific Activity, Electrophoresis, And 08
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University of Mumbai B. E. (Biotechnology) Rev 2016 Page 79
Ultracentrifugation. Immobilization of Enzyme for repeat use.
05 Solid -liquid reactors for enzymes: Types of heterogeneous
reactors and its design criteria (CSTR, PFR, PBR, FBR etc.),
Operational problems, Decline and loss of enzyme efficiency,
Remedies. 07
06 Application of Enzyme in Industries: Foo d, Leather,
Beverage, Detergents, Pharmaceuticals and Medicines,
Analytical/ Diagnostics and Biosensors, Therapeutics. 05
Assessment
Internal
Assessment consists of two tests which should be conducted at proper
intervals.
End Semester theory examination
Question paper will comprise of 6 questions each carrying 20 questions.
Total 4 questions need to be solved
Question no.1 will be compulsory based on entire syllabus wherein sub
questions can be asked.
Remaining questions will be randomly sele cted from all the modules
Weightage of marks should be proportional to number of hours assigned to
each module
References
1. Enzymes -Palmer; T, (Affliated East West Press Pvt. Ltd.), 2004.
2. Biochemistry - Stryer, Berg, 6thEdition, (W.H. Freeman and Co.), 2007.
3. Biochemistry -Metzler; DE, 2nd Edn., (Academic press) 2001.
4. Lehninger Principles of biochemistry -Nesson, Cox, 4th Edn., (W.H. Freeman
and Co.), 2005.
5. Biochemistry Voet & Voet; J, 3rd Edn. (John Wiley and sons Inc.), 2004.
6. Outlines of Biochemistry -Conn; E,Stumpf, 5thEdn.(Tata -McGraw Hill),
1988.
7. Enzyme Biotechnology - Tripathi; G, (ABD Publishers), 2003.
8. Enzyme Technology, M.F. Chaplin and C. Bucke. Cambridge University
Press
9. Industrial Enzymes & their applications, H. Uhlig, (John Wiley and Sons
Inc.)
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University of Mumbai B. E. (Biotechnology) Rev 2016 Page 80
Course Code Course/Subject Name Credits
BTC604 IPR, Bioethics and Biosafety 4
Pre-requisites:
Knowledge of materials to be classified as biohazard, knowledge about
current scenario of biotechnological issues.
Course Objectives:
Tounderstandthelawsgoverningbiotechnologyandrelatedfieldatnationalandint
ernational level.
To gain knowledge about safety precautions necessary during
biotechnological work.
To understand the ethical perspective of handling biomaterials
Course outcomes:
To be aware of rules and regulations setup at international level for various
biotechnology related work so that any further research can be formulated
accordingly.
Toknowthesocialandlegalstateofthesocietywithrespecttogeneticallyengineere
dproducts or other outcomes of biotechnology.
Work according to the safety precautions set up by international bodies while
handling bio hazardous material.
Module Contents Contact
Hours
01 Introduction to Intellectual Property:
Concept of Intellectual Property, Kinds of Intellectual
Property: Patents, Copyrights, Designs, Trademarks,
Geographical Indication. Infringement of IPR, its protection
and remedies. Licensing and its types. 10
02 International Scenario:
Introduction to the leading international instruments
concerning intellectual property rights: The Berne
Convention, GATT, WTO, Indian Patent Act, Universal
Copyright Convention, The Paris Convention, TRIPS, The
World Intellectual Property Rights Organiza tion (WIPO),
Budapest treaty 05
03 Patents:
Requirement of patentable novelty, inventive step, prior art
Classifying products as patentable and non -patentable
Procedure for applying for patent, Patent Infringement and
related case studies, Biological Pa tentability. 06
04 IPR and Biotechnology:
Biopiracy and Bioprospecting, Farmers Rights and Plant
breeders rights, Biodiversity, CBD 04
05 Biosafety:
Good Lab Practices, Introduction to Biological Safety
Cabinets, Primary Containment for Biohazards, B iosafety
Levels, GMOs, LMOs and their environmental impact, Roles
of Institutional Bio safety Committees: RCGM, GEAC etc.
for GMO applications in food and agriculture, Risk analysis, 06
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University of Mumbai B. E. (Biotechnology) Rev 2016 Page 81
assessment and management.
06 Bioethics:
Bioethical issues related to Healthcare & medicine, Food&
agriculture, Genetic engineering. The Human Genome Project
and Genetic Testing, Environmental problems. 04
Term Work
Term work shall consist of minimum eight tutorials from entire syllabus which
are to be given at regular intervals Batch wise.
Tutorials: 20 Marks
Attendance: 05 Marks
Total: 25 Marks
Assessment
Internal
Assessment consists of two tests which should be conducted at proper
intervals.
End Semester theory examination
Question paper will comprise of 6 questions each carrying 20 questions.
Total 4 questions need to be solved
Question no.1 will be compulsory based on entire syllabus wherein sub
questions can be asked.
Remaining questions will be randomly selected from all the modules
Weightage of mark s should be proportional to number of hours assigned to
each module
References
1. IPR, Bio safety and Bioethics by Deepa Goel and Shomini Parasha
2. Intellectual property rights by Dr. Reddy
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University of Mumbai B. E. (Biotechnology) Rev 2016 Page 82
Course Code Course/Subject Name Credits
BTC605 Process Control and Instrumentation 4
Pre-requisites:
Knowledge of Laplace Transforms, Knowledge of differentiation and
Integration
Course Objectives:
To understand the basic concepts of process parameter control
To understand the closed loop and open loop control system
To carry out the stability analysis for a given process
Course outcomes:
Student will be able to design the process control of a parameter.
Student will be able to carry out the stability analy sis for a process.
Module Contents Contact
Hours
01 Instrumentation:
Instrumentation principles, Introduction to temperature and
liquid level measurements, measurement of important
physico -chemical and biochemical parameters, methods of
on-line and off-line biomass estimation, flow injection
analysis for measurement of substrates, products and other
metabolites. 05
02 First order systems:
Process characteristics, Laplace transforms, first order
systems examples, mercury in glass thermometer, liquid level
system, linearization, response of first order system for step,
pulse, impulse and sinusoidal changes in input, conceptual
numericals. 05
03 Firs torder systems in series:
Interacting and non-interacting systems and their dynamic
response to step, pulse and impulse inputs; conceptual
numericals. 04
04 Second order systems:
Second order systems with transfer functions (spring -damper,
control valve, U -tube manometer), response of second ord er
system to step, pulse/ impulse and sinusoidal input
Overdamped, underdamped and critically damped condition
of second order system, transportation lag. 05
05 Controllers and final control elements:
Actuators, Positioners, Valve body, Valve plugs,
Charac teristics of final control elements, controllers two
position control, proportional control, derivative control,
integral control, P -I (proportional - integral) control, P-
D(proportional -derivative) control, P -I-D (proportional -
integral - derivative) control, conceptual numericals. 05
06 Closed loop control systems:
Block diagrams for servo and regulatory problems. Transient
response of first and second order processes for set point 04
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University of Mumbai B. E. (Biotechnology) Rev 2016 Page 83
changes and load changes with proportional and PI
controllers, conceptual numericals.
07 Controller design ands tability:
Criteria for stability, Routhtest; Root locus analysis,
Introduction to frequency response, Qualitative discussion
about Bode criteria and Nyquist criteria, Controller tuning -
Gain & Phase margin; Conceptual numerical on Routh test,
Root locus and Bode plot. 05
08 Bioprocesses dynamics and control:
Dynamics and control of bioreactors & sterilizers. On -line
data analysis for state and parameter estimation, techniques
for biochemical processes, Complex control strategies such as
feed forward, cascade, adapter, supervisory, multivariable
controls and their application for optimum controls. 03
Term Work
Term work shall consist of minimum eight tutorials from entire syllabus which
are to be given at regular intervals Batch wise.
Tutorials: 20 Marks
Attendance: 05 Marks
Total: 25 Marks
Assessment
Internal
Assessment consists of two tests which should be conducted at proper
intervals.
End Semester theory examination
Question paper will comprise of 6 questions each carrying 20 questions.
Total 4 questions need to be solved
Question no.1 will be compulsory based on entire syllabus wherein sub
questions can be asked.
Remaining questions will be randomly selected from all the modules
Weightage of marks should be proportional to number of hours assigned to
each module
References
1. Coughnanowr, Process Systems Analysis and Control.
2. Stephanopoulos, G., Chemical Process Control, Prentice Hall of India., 1990.
3. Richardson, J. F., Peacock, D. G., Coulson & Richardson’s Chemical
Engineering, Vo. 3., ed. 3., Asian Books Pvt. Ltd, New Delhi, 1994.
4. William L. Luyben, Process Modeling Simulat ion and Control for Chemical
Engineers, McGraw Hill International Edition. 1990.
5. B.Wayne Bequette Process Control Modeling, Design and Simulation,
Prentice Hall of India Pvt. Ltd.
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University of Mumbai B. E. (Biotechnology) Rev 2016 Page 84
Course Code Course/Subject Name Credits
BTE6021 Department Elective II- Computational Fluid
Dynamics 4
Pre-requisites:
Linear Algebra
Partial Differential Equations
Scilab or Python
Course Objectives:
To understand the formulation of CFD problems
To discretize the problems
To solve the set of equations in simple cases using Scilab routines.
To understand and use software in CFD
Course Outcomes:
The student will be able to obtain flow profiles for some simple applications
using Scilab.
The student will be able to use appropriate software for solving realistic
problems.
Module Contents Contact
Hours
1 Module: Introduction
Contents: Advantages of Computational Fluid Dynamics
Typical Practical Applications
Equation Structure
Overview of CFD 02
2 Module: Preliminary Computational Techniques
Contents: Discretisation
Approximation to Derivatives
Accuracy of the Discretisation Process
Wave Representation
Finite Difference Method 04
3 Module: Theoretical Background
Contents: Convergence
Consistency
Stability
Solution Accuracy
Computational Efficiency 06
4 Module: Weighted Residual Methods
Contents: General Formulation
Least Squares, Galerkin and Subdomain Formulations.
Weak form of Galerkin Method 08
5 Module: Finite Element Method
Contents: Piece -wise Continuous Trial Functions
One Dimensional Linear and Quadratic Elements 08
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University of Mumbai B. E. (Biotechnology) Rev 2016 Page 85
One Dimensional Heat Transfer
Tri-diagonal Matrix Algorithm
6 Module: Two Dimensional Elements
Quadrilateral Elements
Steady State Heat Transfer in Two Dimensions
Alternating Direction Implicit Method
Potential Flow in Two Dimensions 08
7 Module: Finite Volume Method
One Dimensional Diffusion
Two Dimensional Diffusion
Diffusion With Convection and The Upwind Scheme 06
8 Module: Pressure Velocity Coupling in Steady Flows
The Staggered Grid
The Momentum Equation
The Simple Algorithm 06
Term Work
Term work shall consist of minimum eight tutorials from entire syllabus which
are to be given at regular intervals Batch wise.
Tutorials: 20 Marks
Attendance: 05 Marks
Total: 25 Marks
Assessment
Internal
Assessment consists of two tests which should be conducted at proper
intervals.
End Semester theory examination
Question paper will comprise of 6 questions each carrying 20 questions.
Total 4 questions need to be solved
Question no.1 will be compulsory based on entire syllabus wherein sub
questions can be asked.
Remaining questions will be randomly selected from all the modules
Weightage of marks should be proportional to number of hours assigned to
each module
Text Books
1. C.A.J. Fletcher; Computational Techniques for Fluid Dynam ics 1; Springer -
Verlag Berlin Heidelberg GmbH
2. P. Seshu; Textbook of Finite Element Analysis; PHI Learning Private
Limited, New Delhi
3. H.K. Versteeg and W. Malalasekera; An Introduction To Computational
Fluid Dynamics; Longman Scientific & Technical
Referenc es
1. John D. Anderson; Computational Fluid Dynamics; McGraw Hill Education
Private Limited
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University of Mumbai B. E. (Biotechnology) Rev 2016 Page 86
Course Code Course/Subject Name Credits
BTE6022 Department Elective II - Protein Engineering 4
Pre-requisites:
Knowledge of Biochemistry, Recombinant DNA Technology and
Enzyme Engineering
Course Objectives:
Imparting knowledge about structure function relationships of proteins
Studying the problem of protein folding and methods of characterization
folded proteins
Aspects of Protein Engineering in the industry
Course outcomes:
At the end the student would have learned:
Structure and Function relationship in proteins and its application in
designing proteins
Process of engineering proteins to increase its value by assisting folding,
purification
Prote in engineering of therapeutic proteins, industrially important
enzymes and antibodies.
Module Contents Contact
Hours
01 Primary, Secondary, Tertiary and Quaternary Structure of
Proteins,
Bonds that stabilize a protein molecule,
Ramchandran Plot.
Protein folding pathways and Energy Status of a Protein
Molecule, Protein Degradation in the cell 06
02 Techniques involved in studying protein structure:
Methods of protein crystallization.
Methods to study the quaternary structures of proteins: X -
ray Crystall ography, NMR Spectroscopy.
MALDI -TOF, ESI -MS 07
03 Structure Function Relationships in Proteins:
Helix -turn-Helix motif in DNA binding and homeo
domain protein, Zinc fingers, Leucine zippers.
Membrane proteins: General characteristics,
Transmembrane segments, bacteriorhodopsin and
Photosynthetic reaction center 06
04 Concepts of designing a new Protein Molecule:
Chemical synthesis of peptides.
Target molecules for Protein Engineering.
The protein cycle and steps involved in Engineering a new
Protei n.
De novo protein design 06
05 Applications of Protein Engineering:
Protein Engineering to enhance the solubility and assist 10
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University of Mumbai B. E. (Biotechnology) Rev 2016 Page 87
folding of expressed proteins.
Protein Engineering to assist purification of expressed
proteins.
Role in Vaccine Development.
Engineering blood clotting factors:f actor VIII.
Engineering enzymes: tyrosyl - t RNA synthase.
Engineering therapuetic hormones: insulin.
Engineering humanized antibodies
Term Work
Term work shall consist of minimum eight tutorials from entire syllabus
which are to be given at regular intervals Batch wise.
Tutorials: 20 Marks
Attendance: 05 Marks
Total: 25 Marks
Assessment
Internal
Assessment consists of two tests which should be conducted at proper
intervals.
End Semester theory examination
Question paper will comprise of 6 questions each carrying 20 questions.
Total 4 questions need to be solved
Question no.1 will be compulsory based on entire syllabus wherein sub
questions can be asked.
Remaining questions will be randomly selected from all the modules
Weightage of marks should be proportional to number of hours assigned
to each module
References
1. Lilia Arbenghina; Protein Engineering in Industrial Biotechnology;
Harwood Academic Publishers
2. Creghton TE; Proteins Function, A Practical Approach; Freeman WH,
Second Ed, 1993
3. Branden C. And Tooze J.; Introduction to Protein Structure; Second
Edition, Garland Publishing, NY, USA, 1999
4. Moody PCE, and A.J. Wilkinson; Protein Engineering; IRL Press,
Oxford, 1990
5. Walsh.G; Protein Biotechnology and Biochemistry; 2nd ed.; Wiley
Publications
6. Klaus Demobowsky, Novel Therapeutic Proteins; Wiley Publications
7. Voet D. and Voet G.; Biochemistry’ Third Edn. John Wiley and Sons,
2001
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University of Mumbai B. E. (Biotechnology) Rev 2016 Page 88
Course Code Course/Subject Name Credits
BTE6023 Elective II - Cancer Biology 4
Pre-requisites:
Knowledge of Biochemistry, Cell biology, Immunology, Molecular biology,
Genetic Engineering
Course Objectives:
Studying cellular and molecular mechanisms that are deregulated in
cancerous cells.
Contribution of Genetic mutation in the development of cancer
Environmental factors influencing susceptibility of cancer
Treatment modalities i.e traditional chemotherapies and novel targeted
therapeutic approaches
Genetic Engineering technologies t o understand cancer prevention,
diagnosis, and treatment
Course outcomes:
By the end of the course, students should be able to describe the:
Process of tumorigenesis at the molecular and cellular level.
Cell cycle regulatory mechanisms in normal and tumor cells
Role of oncogenes and tumor suppressor genes and their genetic alterations
in cancer formation.
Importance of apoptosis in normal and tumor cells
Tumorigenesis, angiogenesis, and metastasis.
Module Contents Contact
Hours
01 Control of Cell proliferation, Cell Cycle and Cell Death:
Process of Cell cycle, tumor suppressor genes, Cell cycle
control and pRb tumor suppressor. Apoptosis and p53 tumor
suppressor gene. Introduction to different forms of cancers. 06
02 Mutagens, carcinogens, and mut ations:
Chemical Carcinogenesis, Metabolism of Carcinogenesis,
targets of Chemical Carcinogenesis,
Physical carcinogens - X-Ray radiation – Mechanism of
radiation Carcinogenesis.
DNA repair mechanisms – DNA repair defects and their
relationship to cancer.
Gene mutations and deregulation in cell signal pathway 08
03 Oncogenes, growth factors, receptors and cancer:
Identification of Oncogenes, Retroviruses and Oncogenes,
detection of Oncogenes,
Role of growth factors and receptors in carcinogenesis, RAS ,
NFkB, Wnt signaling in cancer. 08
04 Tumor Invasion and Metastasis:
Metastatic cascade, Basement membrane disruption, Three
step theory of invasion, Proteinases and tumour cell invasion.
Multi -step tumorigenesis and the evolution of cancer. 07
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University of Mumbai B. E. (Biotechnology) Rev 2016 Page 89
05 Cancer treatment modalities:
Different forms of therapy - Chemotherapy, Radiation Therapy,
Immunotherapy, commonly used cancer diagnostic and
prognostic molecular markers,
Novel targeted therapeutic approaches. 06
Term Work
Term work shall consist of minimum eight tutorials from entire syllabus which
are to be given at regular intervals Batch wise.
Tutorials: 20 Marks
Attendance: 05 Marks
Total: 25 Marks
Assessment
Internal
Assessment consists of two tests which should be conducted at proper
intervals.
End Semester theory examination
Question paper will comprise of 6 questions each carrying 20 questions.
Total 4 questions need to be solved
Question no.1 will be compulsory based on entire syllabus wherein sub
questions can be asked.
Rem aining questions will be randomly selected from all the modules
Weightage of marks should be proportional to number of hours assigned to
each module
References
1. King R.J.B., “Cancer Biology”, Addision Wesley Longmann Ltd, U.K.,
1996.
2. Ruddon. R.W, “Cancer Biology”, Oxford University Press, Oxford, 1995 ,
2007 .
3. Robert Allan Weinberg, “The Biology of Cancer”, Volume 2, Garland
Science, 2007.
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University of Mumbai B. E. (Biotechnology) Rev 2016 Page 90
Course Code Course/Subject Name Credits
BTE6024 Department Elective II - Green Technology 4
Pre-requisites:
Basics of physics, chemistry, biochemistry, and microbiology
Course Objectives:
Green Technologies is a highly interdisciplinary degree program that
emphasizes green systems and the environment, energy technology and
efficiency, and sustainability and society. The objective of this course is to:
Seek opportunities for alternative sourcing, conservation, efficiency and
repurposing through an understanding of product life cycles from origins to
recycling or inevitable disposal.
To design products, processes and complex infrastructure systems to promote
sustainable attributes of importance to the environment and the global
community.
To combine technical and scientific skills with an understanding of the
environment, renewable energy management, waste utilization , resource
management and land based industries who can contribute to the national and
global development.
Course outcomes:
To understand the principles of green chemistry and engineering.
To design processes those are benign and environmentally viable.
To design processes and products those are safe and hazard free.
To learn to modify chemical processes making hazardous products and make
them green safe and economically acceptable by using biotechnology.
Module Contents Contact
Hours
01 Fundamentals of Green Chemistry and Technology - Principles
of Green Chemistry and technology, green chemistry metrics
(atom economy, atom efficiency, E -factor, and other green
chemistry metrics) 05
02 Catalysis - Introduction to catalysis, Catalytic cycle, TON, TOF,
bio-catalysis 03
03 Industrial Safety and Hazard analysis - Introduction to ISO
standards, hazard identification, life cycle analysis, and safety
aspects related to transport, handling and storage of hazardous
chemicals. green technologies for addressing th e problems of
Water, Energy, Health, Agriculture and Biodiversity - WEHAB
(eco-restoration/ phyto -remediation, ecological sanitation,
renewable energy technologies, industrial ecology, agro ecology
and other appropriate green technologies , global warming;
greenhouse gas emissions, impacts, mitigation and adaptation 10
04 Green processes - Microwave assisted reactions, ultra -sonication
assisted reactions, ionic liquids as solvent, water as a reaction
medium, solvent free reactions, supercritical solvents, safe
product and process design, case studies 04
05 Advances in separation process - Adsorption, Distillation, 04
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University of Mumbai B. E. (Biotechnology) Rev 2016 Page 91
filtration, membrane separation, precipitation, crystallization
06 Green Biotechnology - Green concepts in biotechnology, organic
synthesis using supported microbes and enzymes in
biopharmaceuticals/ bio refineries, bioreactor designs,
downstream processing. 04
07 Green Nanotechnology - Nanomaterials for water treatment,
nanotechnology for renewable energy, nanotechnology for
environmental remediation and waste Management,
nanotechnology products as potential substitutes for harmful
chemicals, environmental concerns with nanotechnology 05
Term Work
Term work shall consist of minimum eight tutorials from entire syllabus which are
to be given at regular intervals Batch wise.
Tutorials: 20 Marks
Attendance: 05 Marks
Total : 25 Marks
Assessment
Internal
Assessment consists of two tests which should be conducted at proper
intervals.
End Semester theory examination
Question paper will comprise of 6 questions each carrying 20 questions.
Total 4 questions need to be solved
Question no.1 will be compulsory based on entire syllabus wherein sub
questions can be asked.
Remaining questions will be randomly selected from all the modules
Weightage of marks should be proportional to number of hou rs assigned to
each module
References
1. Green Chemistry – An introductory text - M. Lancaster, RSC
2. Industrial catalysis – optimizing catalysts and processes – R J Wijngarden
Wiley - VCH
3. Safety and Reliability of Industrial Products, Systems and Structures C.
Guedes Soares (Eds), CRC Press
4. Green separation processes - C. A. M. Afonso, J. G. Crespo (Ed) - Wiley VCH
5. Industrial biotechnology - sustainable growth and economic success - Wilm
Soetaert Reic J Wandamme - Wiley VCH
6. Energy, Ecology and the Envi ronment, Academic Press Inc, B. R Wilson & W
J Jones, 2005
Page 92
University of Mumbai B. E. (Biotechnology) Rev 2016 Page 92
Course Code Course/Subject Name Credits
BTL601 Lab-II 1.5
Concept for Experiments
A minimum of TEN experiments must be performed
Maintenance of asceptic condition in PTC Lab
Medium Preparations
Callus induction and Regenerations
Callus propagation
Organogenesis
Haploid Culture
Embryo Culture
Somatic Embryogenesis
Suspension Culture
Anther culture for production of haploid plants
In vitro seed germination
Inoculate the tissue culture raised shoots on suitable medium for in vitro
rooting
Hardening and acclimatization of in vitro raised rooted shoots
Hairy root induction by Agrobacterium tumefaciens
seed anti -mitotic assay
Meristem culture for obtaining Virus free plan ts
Effect of plant growth regulators on callus induction : e ffect of hormone
variation
Encapsulate the shoot buds, seeds to demonstrate the production of synthetic
seeds
Sterilization procedures and media preparation for Animal Cell cultures
Establishme nt of Primary cell culture from chick embryo
Animal cell culture: viable cell counting by Haemocytometer
Practical Examination
Duration for practical examination would be the same as assigned to the
respective lab per week.
A student becomes eligible for practical examination after completing a
minimum of eight out of ten experiments.
Page 93
University of Mumbai B. E. (Biotechnology) Rev 2016 Page 93
Course Code Course/Subject Name Credits
BTL602 Lab-III 1.5
Concept for Experiments
A minimum of TEN experiments must be performed
Isolation of enzyme from a plant source
Isolation of enzyme from an animal source
Isolation of intracellular enzyme
Determination of specific activity of enzyme
Determination of the optimum pH & temperature of enzyme
Determine the stability of enzyme
Immobilization of enzyme
Determination of kinetic parameters (Km and Vmax)
Purification of enzymes
Studies of various enzyme reactors
Bacteriological testing of milk (MBRT)
Estimation of Calcium by EDTA method
Isolation and separation of chloroplast by sucrose density gradient
centrifugation
Production of Grape wine and is biochemical analysis
Determination of starch and sugar in plant tissue
Clarification of fruit juices
Study of pectinase activity
Primary screening of Amylase producing bacteria and f ungi from soil
Practical Examination
Duration for practical examination would be the same as assigned to the
respective lab per week.
A student becomes eligible for practical examination after completing a
minimum of eight out of ten experiments.
Page 94
University of Mumbai B. E. (Biotechnology) Rev 2016 Page 94
University of Mumbai
Program Structure for B.E. Biotechnology (Revised 2016 )
B.E. Semester VII (w.e.f 2019 -2020)
Course
code Course Name Teaching Scheme
(Contact Hours) Credits Assigned
Theory Practical Tutorial Theory Practical Tutorial Total
BTC701 Bioseperation& Downstream
Processing Technology -I 4 - 1 4 - 1 5
BTC702 Bioprocess Modelling and
Simulation 4 - 1 4 - 1 5
BTC703 Agriculture Biotechnology 3 - 1 3 - 1 4
BTE703X Department Elective III 3 - 1 3 - 1 4
ILO701X Institute Level optional Subject I 3 - - 3 - - 3
BTP701 Project A - - 6 - - 3 3
BTL701 Lab - IV - 3 - - 1.5 - 1.5
BTL702 Lab - V - 3 - - 1.5 - 1.5
Total 17 6 10 17 3 7 27
Course
code Course Name Examination Scheme
Theory Term
Work Pract/
Oral Oral Total
Internal
Assessment End
Sem
Exam Exam
Duration
(in hrs)
Test 1 Test 2 Avg
BTC701 Bioseperation& Downstream
Processing Technology -I 20 20 20 80 3 25 - - 125
BTC702 Bioprocess Modelling and
Simulation 20 20 20 80 3 25 - - 125
BTC703 Agriculture Biotechnology 20 20 20 80 3 25 - - 125
BTE703X Department Elective III 20 20 20 80 3 25 - - 125
ILO701X Institute Level optional Subject I 20 20 20 80 3 - - - 100
BTP701 Project A - - - - - 100 50 150
BTL701 Lab - IV - - - - - - 25 - 25
BTL702 Lab - V - - - - - - 25 - 25
Total 100 400 - 200 50 50 800
Department Elective III (Sem VII)
Engineering Stream Advanced Science Stream Technology Stream
1. Stem Cell & Tissue
Engineering (BTE7031) 1. Operation research in
Biotechnology (BTE7032)
2. Project Management (BTE7033) 1. Nanotechnology (BTE7034)
Institute Level Optional Subject I (Sem VII)
1. Product Lifecycle
Management (ILO7011) 4. Design of Experiments
(ILO7014) 7. Disaster Management and
Mitigation Measures (ILO7017)
2. Reliability Engineering
(ILO7012) 5. Operation Research
(ILO7015) 8. Energy Audit and Management
(ILO7018)
3. Management Information
System (ILO7013) 6. Cyber Security and Laws
(ILO7016) 9. Development Engineering
(ILO7019)
Page 95
University of Mumbai B. E. (Biotechnology) Rev 2016 Page 95
Course Code Course/Subject Name Credits
BTC701 Bio separation and Downstream Processing
Technology -I 5
Pre-requisites:
Basics of Bioprocesses and Unit Operations
Basic knowledge of mass balance
Concepts of molecular diffusion and diffusion coefficients
Course Objectives:
To cover the fundamentals, and design concepts of various downstream
purification steps ( unit operations) involved in a biochemical process.
Course outcomes:
Students will be able to describe theory, principle, design, application and
possible integrations of unit operations in bioprocessing.
Module Contents Contact
Hours
01 Introduction to By-products and Bio separation:
Range and characteristics of bio products, Characteristics of
Fermentation Broth, Selection of unit operation with due
consideration of physical, chemical and biochemical aspect
of biomolecules. Stages of Downstream Processing 04
02 Product release and recovery processes:
Fundamental principles of obtaining the product from cell
cultures: intracellular vs. extracellular product.
Cell disruption -Physical, Chemical and Enzymatic methods
of cell disruption, Mechanical Cell disruption methods: High
pressure Cell Homogenizer, Bead Mill, Son ication 05
03 Primary Separation:
Removal of insolubles and Biomass (and particulate debris)
separation techniques, Flocculation and sedimentation,
Centrifugation -Ultracentrifugation, Gradient centrifugation,
Filtration: Theory of Filtration, Pre -treatmen t of
Fermentation Broth, Filter Media and Equipment,
Conventional and Cross -flow Filtration, Continuous
Filtration, Filter cake resistance, specific cake resistance,
Washing and dewatering of filter cakes 09
04 Gas Absorption:
Solubility of gases in liquids, Effect of temperature and
pressure on solubility, Ideal and Non-ideal solutions, Choice
of solvent for gas absorption, absorption factor, stripping
factor, minimum gas liq ratio, Single stage gas absorption -
Cross Current, Co - current, Countercurrent, Multistage
Counter current Operation, Absorption with Chemical
Reactions,
Related problems 10
05 Liquid -Liquid Separation Process:
Introduction to Liquid -Liquid Extraction, Choice of Solvent
for Liquid -Liquid Extraction, Binodal solubility curve, 10
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University of Mumbai B. E. (Biotechnology) Rev 2016 Page 96
Single Stage Operation, Equipments for liquid -liquid
extraction.
Types of extraction processes: Reactive extraction, Aqueous
two phase systems, Reverse micellar extraction, Liquid -
liquid and solid -liquid extraction, Supercritical fluid
Extraction.
Design of extr action equipment. Different types of extractors
and designing of extractors.
Distillation: Simple, Steam and Equilibrium distillation,
Fractionation, Mccabe Thiele method, azotropes, numericals
06 Leaching and Precipitation:
Leaching: Representation of equilibria, single stage leaching,
and multistage cross current leaching, multistage counter
current leaching, equipments for leaching.
Precipitation: Protein Precipitation methods: Isoelectric
precipitation, Salting out, Organic solvent addition, Non-
ionic polymers, Polyelectrolyte Addition, Selective
denaturation of unwanted proteins, Large scale precipitation,
Applications 07
Term Work
Term work shall consist of minimum eight tutorials from entire syllabus which
are to be given at regular intervals Batch wise.
Tutorials: 20 Marks
Attendance: 05 Marks
Total: 25 Marks
Assessment
Internal
Assessment consists of two tests which should be conducted at proper
intervals.
End Semester theory examination
Question paper will comprise of 6 questions each carrying 20 questions.
Total 4 questions need to be solved
Question no.1 will be compulsory based on entire syllabus wherein sub
questions can be asked.
Remaining questions will be randomly selected from all the modules
Weightage of marks should be proportional to number of hours assigned to
each module
References
1. Treybal R.E. , Mass transfer operation, 3 Ed., McGraw Hill New York,
1980.
2. McCabe W. L.and Smith J.C., Unit operation in chemical engineering, 5Ed.,
McGraw Hill New York 1993.
3. Geankoplis C.J., Transport processed and unit operations, Prentice Hall,
New Delhi 1997.
4. Roger G. Harrison, Paul Todd, ScottR. Rudge, Demetri P. Petrides,
Bioseparations Science and Engineering, Oxford University Press
5. B.Shivshankar, Bioseparations: Principles and Techniques, Eastern Economy
Page 97
University of Mumbai B. E. (Biotechnology) Rev 2016 Page 97
Edition, PHI Learning Pvt. Ltd., Publishing House, New Delhi, 2012
6. Bioseparation & bioprocessing (2nd Ed.) 2-Volume set, Ed
SUBRAMANIAN Ganapathy, Wiley -VCH, ( 09 -2007)
7. P.A. Belter, E.L. Cussler and Wei -Shou Hu., Bioseparations -Downstream
Processing for Biotechnology, Wiley Interscience Publication, 1988.
8. J.E. Baileyand D.F. Ollis, Biochemical Engineering Fundamentals, 2nd
Edition, McGraw Hill, Inc., 1986.
9. R. K. Scopes, Berlin, Protein Purification: Principles a nd Practice, Springer,
1982.
10. Scopes Ak, Protein Purification, IRL Press, 1993
11. Biotechnology: Bioprocessing, Rhem and Reed, Vol. 3, 1993
12. Separation and purification techniques in biotechnology, Fredreich Dechow,
1989
13. Asenjo J.A. and J.Hong (Eds), Separation Processes in Biotechnolgy, Taylor
and Francis
14. T. Schepler et al, Biotreatment, Downstream Processing and Modeling
(Advances in Biochemical Engineering /Biotechnology, Vol 56) by Springer
Verlag
Page 98
University of Mumbai B. E. (Biotechnology) Rev 2016 Page 98
Course Code Course/Subject Name Credits
BTC702 Bioprocess Modelling and Simulation 5
Pre-requisites:
Knowledge of Fundamental Laws of Physics
Knowledge of basic Mathematics
Knowledge of Reactors and its types
Knowledge of production of various fermentation products
Course Objectives:
To understand the mathematical models in Biochemical Engineering systems
To learn about di fferent apects of modelling in Bioprocess system
To learn various techniques to solve and simulate various bioprocess models
Course outcomes:
Students will be able to formulate model for biochemical System.
Students will be able to solve Biochemical models
Module Contents Contact
Hours
01 Basic Modelling Principles: Introduction, defin etion of
Modelling and simulation, different types of models,
application of mathematical modelling.
Fundamental laws: continuity equation, energy equation,
equation of motion, transport equation, equation of state,
Phase and chemical equilibrium, chemical kinetics with
examples 08
02 Mathematical Models for Biochemical Engineering
Systems: Batch Reactor, CSTR isothermal with
cooling/heating jacket or coil, Continuous Stirred Tank
Bioreactor, Fed Batch reactor, Batch distillation 08
03 Numerical Methods:
Solution of linear algebraic equations by Cramer’s rule,
Gauss elimination, Gauss siedel iterative method. Solution
of Non algebraic equations by Bisection method, Newton
Raphson, Secant Method.
Numerical integratio n: Trapezoidal rule, Simpson’s1/3rule,
Simpson’s 3/8 rule, Euler’s method, Runga Kutta method,
Basic data analysis -curve fitting 10
04 Modelling approaches for Biological systems
Growth
kinetic Models - structured and unstructured systems;
Compartment models; Deterministic and stochastic
approaches for modelling structured systems. Thermal death
kinetics models, Stochastic Model for thermal sterilization
of medium. 10
05 Modelling for activated sludge process, Model for anaerobic
digestion, Model for l actic acid fermentation, antibiotic
production, Ethanol fermentation 09
Page 99
University of Mumbai B. E. (Biotechnology) Rev 2016 Page 99
Term Work
Term work shall consist of minimum eight tutorials from entire syllabus which
are to be given at regular intervals Batch wise.
Tutorials: 20 Marks
Attendance: 05 Marks
Total: 25 Marks
Assessment
Internal
Assessment consists of two tests which should be conducted at proper
intervals.
End Semester theory examination
Question paper will comprise of 6 questions each carrying 20 questions.
Total 4 questions need to be solved
Question no.1 will be compulsory based on entire syllabus wherein sub
questions can be asked.
Remaining questions will be randomly selected from all the modules
Weightage of marks should be proportional to number of hou rs assigned to
each module
References
1. J.E. Baileyand , D.F. Ollis, Biochemical Engg Fundamentals, 1986, McGraw
Hill Book Company
2. Said S.E.H. Elnashaie, Parag Garhyan, Conservation Equations and
Modeling of Chemical and Biochemical Processes, 2003, Marcel Dekker
3. B. Wayne Bequette, Process Dynamics: Modeling, Analysis and Simulation,
1998, Prentice Hall
4. Michael Shuler and Fikret Kargi, Bioprocess Engineering: B asic
Concepts,2nd Edition, Prentice Hall, Englewood Cliffs, NJ, 2002
5. Process Modelling, Simulation and Control for Chemical Engineers, by
William Luyben, McGraw Hill, Second Edition.
6. Numerical Methods and Modelling for Chemical Engineers, Davis
M.E. ,Wil ey, New York 1984
7. Numerical Methods for Engineers, Santosh Kumar Gupta, Tata McGraw hill,
1995
8. Numerical Methods, M. K. Jain, S. R. K. Iyengar, and R. K. Jain Sixth
Edition. New Age International Publishers, New Delhi, 2012
9. Introduction to Chemical Engin eering Computing by Bruce A. Finlayson
Wiley - International, 2005.
Page 100
University of Mumbai B. E. (Biotechnology) Rev 2016 Page 100
Course Code Course/Subject Name Credits
BTC703 Agriculture Biotechnology 4
Pre-requisites:
Knowledge about plant tissue culture methods and applications.
Knowledge
aboutgeneticengineeringmethodsfore.g.genetransfertechniques,plant vectors
and basics of transgenic plants.
Knowledge about traditionally used herbicides, pesticides, its advantages and
drawbacks
Knowledgeaboutethicalandbiosafetyissuesandintellectualpropert yrulesassoci
ated
with plants
Course Objectives:
To understand basic plant biology and breeding methods.
To gain knowledge about transgenic plant analysis, principle behind
generation of herbicide and pest tolerant plants.
To understand the stress condi tion in plants and methods to overcome it.
To design methods for crop improvement.
To analyse applications based on molecular farming.
Course outcomes:
Students will be able to:
Apply the transgenic methods to develop better quality crops.
Understand the advantages and drawbacks of engineered plants and modify
them accordingly.
Harness the plants for improved quality biomaterials.
Module Contents Contact
Hours
01 Agricultural Microbiology:
Microbial groups in soil, Plant and Microbe interactions.
Plant pathogens, Biological nitrogen fixation, Microflora of
Rhizosphere and Phyllo sphere microflora, microbes in
composting, Beneficial microorganisms in Agriculture:
Biofertilizer (Bacterial Cyanoba cterial and Fungal), microbial
insecticides, Microbial agents for control of Plant diseases 05
02 Plant Breeding:
Historical milestones in plant breeding, Aims and objectives
of plant breeding, Significance of plant breeding in crop
development. Concepts in plant breeding – Simple versus
Complex Inheritance, Mating Systems, Varieties, and Pure
Lines. Methods of Plant Breeding. Plant Hormone Signal
Transduction - Auxin and GA Signaling, Cytokinin and
Ethylene Signaling 05
03 Transgenic Plants:
Transgenic P lant Analysis: screening on selection media,
PCR, Intact Transgene Integration characterization, Real time
PCR, Transgene expression, western blot analysis. 05
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University of Mumbai B. E. (Biotechnology) Rev 2016 Page 101
Regulations and Biosafety. Field Testing of Transgenic
Plants - Environmental Risk Assessment (ERA) process, e.g.
the case of Bt Maize, Agronomic Performance, Risk analysis.
Clean -gene technology.
04 Genetic manipulation of herbicide tolerance:
The use of herbicides in modern agriculture,
types of compounds used as herbicides,
Strategies for engineering herbicide tolerance - Glyphosate
tolerance, Phosphinothricin,
Prospects for plant detoxification systems,
Commercialization of herbicide -tolerant plants to date,
The environmental impact of herbicide -tolerant crops,
Development of Superweeds. 03
05 Biotic and Abioticstress:
Abiotic stress: Acclimatization and crop adaptation to water
stress, salinity stress, temperature stress, heat and cold, Photo
oxidative stress, nutrient stress, heavy metal stress, metabolite
engineering for abiotic stress t olerance Biotic stress: plant
response to pathogens and herbivores, biochemical and
molecular basis of host plant resistance, toxins of fungi and
bacteria, systemic and induced resistance, pathogen derived
resistance, genetic engineering for biotic stress resistance 06
06 Genetic manipulation of pest resistance:
The nature and scale of insect pest damage to crops.
GM strategies for insect resistance: the Bacillus thuringiensis
approach. The use of Bacillus thuringiensisas a biopesticide.
Bt-based genetic modification of plants . Problem of insect
resistance to Bt, environmental impact of Bt crops.
Copy Nature strategy 03
07 Improvement of crop yield and quality:
Genetic manipulation of fruit ripening, softening, genetic
modification of ethylene biosynthesis. Golden rice and
Biofortified rice. Engineering plant protein composition for
improved nutrition. The genetic manipulation of crop yield by
enhancement of photosynthesis 04
08 Molecular farming:
Farming of carbohydrates (e.g.starch, polyfruct ans) Metabolic
engineering of Lipids (e.g. Bioplastics) Molecular farming of
proteins (e.g.oleosin system:hirudinand insulin production).
Medically related proteins (e.g.custom made antibodies,
Edible vaccines) 04
Term Work
Term work shall consist of minimum eight tutorials from entire syllabus which
are to be given at regular intervals Batch wise.
Tutorials: 20 Marks
Attendance: 05 Marks
Total: 25 Marks
Page 102
University of Mumbai B. E. (Biotechnology) Rev 2016 Page 102
Assessment
Internal
Assessment consists of two tests which should be conducted at proper
intervals.
End Semester theory examination
Question paper will comprise of 6 questions each carrying 20 questions.
Total 4 questions need to be solved
Question no.1 will be compulsory based on entire syllabus wherein sub
questions can be asked.
Rem aining questions will be randomly selected from all the modules
Weightage of marks should be proportional to number of hours assigned to
each module
References
1. Plant biotechnology -The genetic manipulations of plants by Slater, A., Scott,
N. and Fowler, M., Oxford University press
2. Principles of Plant Breeding by Allard R W 1960 .Kalyani Publishers, New
Delhi.
3. Plant Biotechnology and Genetics: Principles, Techniques, and Applications
- Edited by C. Neal Stewart, Jr.
4. Stress biology, by U.Chakrabo rty, Bishwanath Chakraborty , 2005 . Narosa
Publishing House.
5. Agricultural Microbiology by D. J. Bagyaraj, G. Rangaswami, Prentice Hall
of India Pvt Ltd.
Page 103
University of Mumbai B. E. (Biotechnology) Rev 2016 Page 103
Course Code Course/Subject Name Credits
BTE7031 Department Elective -III: Stem Cell & Tissue
Engineering 4
Pre-requisites:
Cell Biology, Developmental Biology, Biochemistry, Molecular Biology
and Genetics.
Course Objectives:
To understand the developmental processes in a complex living system.
To manipulate the cells to change and perform tasks in a carefully directed
fashion.
To understand the possibilities this technology o ffers in a medical field
Course outcomes:
This course gives an overview about the application of stem cells for
regenerat ive medicine.
It explains advantages and disadvantages of stem cells as therapeutics.
The students obtain knowledge in medical applications of biomaterials as
well as about basic concepts regarding design and mechanical properties of
selected natural and s ynthetic biomaterials
The Tissue Engineering and Regenerative Medicine educate students in two
new, interdisciplinary fields in the biomedical sciences that aim to replace
damaged tissue in the human body and to stimulate the body’s own
regenerative proces ses.
International research and development of new therapies in these areas is
currently booming
Module Contents Contact
Hours
01 Development of multicellular organisms: Universal
mechanisms of animal development, Basic anatomical
features, Role of proteins and regulatory DNA in
development, Approaches to understand developmental
processes, Cell fate, Positional value of cell, Inductive
signalling, Asymmetric cell division, Positive feedback,
Morphogens and their gradients, Intrinsic programming,
Patterning by sequential induction 06
02 Stem cells: Definition, Properties & Types of stem cells,
Maintaining population of stem cells, Transit amplifying
cells. Embryonic stem cells: Properties, Pluripotent stem
cells and methods of generating them. Adult stem cells and
their sources. 04
03 Epidermal renewal by stem cells: The multilayered structure
of epidermis, Role of stem cells and transit amplifying cells
in epidermis renewal. Renewal by multipotent stem cells:
Blood cell formation, Bone marrow and Hemopoietic Stem
Cells, Hematopoiesis, Contact Signals From Stromal Cells.
Osteogenesis: Formation of bone & cartilage from
Mesenchymal Stem Cells. 05
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University of Mumbai B. E. (Biotechnology) Rev 2016 Page 104
04 Applications of stem cells in regenerative medicine:
Repairing Nervous system, Liver cell proliferati on and
repair, Cardiac repair, Diabetes treatment, GM stem cells and
Gene therapy. Ethical issues associated with stem cells 05
05 Tissue Engineering: Introduction to biomaterials and tissue
engineering, Elements of biomaterials, Self -assembly and
growth, Mechanical concepts in biomaterials, Different
protein fibers: collagen, silk, keratin. Characterization of
biomaterials, Methods for the determination of
biocompatibility, Biological composite materials e.g. fibers,
Hierarchical design bone, wound care und suture materials,
vascular implants, biomimetic and bio -inspired materials,
Basic techniques to manufacture scaffolds from raw
biomaterials and different prerequisites for the biomaterials 10
06 Tissue engineering examples: Bone & Cartilage tissue
engineering, skin tissue engineering, vascular tissue
engineering, heart valves tissue engineering 05
Term Work
Term work shall consist of minimum eight tutorials from entire syllabus which
are to be given at regular intervals Batch wise.
Tutorials: 20 Marks
Attendance: 05 Marks
Total: 25 Marks
Assessment
Internal
Assessment consists of two tests which should be conducted at proper
intervals.
End Semester theory examination
Question paper will comprise of 6 questions each carrying 20 questions.
Total 4 questions need to be solved
Question no.1 will be compulsory based on entire syllabus wherein sub
questions can be asked.
Remaining questions will be randomly selected from all the modules
Weightage of marks should be proportional to nu mber of hours assigned to
each module
References
1. Molecular biology of Cell: Alberts et al.2014.(Chapter 22 and 23)
2. Gordana Vunjak - Novakovic, R. Ian Freshney (2006): Culture of Cells for
Tissue Engineering, Wiley
3. Ulrich Meyer, Thomas Meyer, Jörg Handsche l, Hans Peter Wiesmann
(2009): Fundamentals of Tissue Engineering and Regenerative Medicine,
Springer
4. Lanza RP, Langer R, Vacanti J. Principles of Tissue Engineering. Third
edition. Academic Press. 2007
Page 105
University of Mumbai B. E. (Biotechnology) Rev 2016 Page 105
Course Code Course/Subject Name Credits
BTE7032 Department Elective -III: Operation Research in
Biotechnology 4
Pre-requisites:
Linear Algebra
Computer Programming
Course Objectives:
To understand Linear Programming and its applications to OR models.
To understand and solve network models in OR.
To understand Game theory and its applications.
To study and design Queuing systems.
Course Outcomes:
The student will be able to solve typical OR models using linear integer and
dynamic programming techniques.
The student will be able to model and solve net work flow problems in OR.
The student will be able to make decisions under various scenarios.
The student will be able to design Queuing Systems
Module Contents Contact
Hours
1 Module: Linear Programming
Contents: Introduction
Graphical Method of Solution
Simplex Method
Two-Phase Method
Duality
Dual Simplex
Revised Simplex 10
2 Module: Transportation Models
Contents: Examples of Transportation Models
The Transportation Algorithm
The Assignment Model
The Trans shipment Model 06
3 Module: Network Models
Contents: Scope and Definition of Network Models
Minimal Spanning Tree Algorithm
Shortest Route Problem
Maximal Flow Model 06
4 Module: Integer and Dynamic Programming
Contents: Branch and Bound Method
Travelling Salesman Problem
Introduction to Dynamic Programming
Forward and Backward Recursion
Selected Applications 06
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University of Mumbai B. E. (Biotechnology) Rev 2016 Page 106
5 Module: Deterministic Inventory Models
Contents: Classic EOQ Model
EOQ with Price Breaks
Dynamic EOQ Models
No-Setup Model
Setup Model 06
6 Module: Decision Analysis and Game Theory
Contents: Decision Making under Certainty
Decision Making under Risk
Decision Under Uncertainty
Game Theory 06
7 Module: Queuing Systems
Contents: Elements of a Queuing Model
Role of Exponential Distribution
Pure Birth and Death M odels
Generalized Poisson Queuing Model
Measures of Performance 08
Term Work
Term work shall consist of minimum eight tutorials from entire syllabus which
are to be given at regular intervals Batch wise.
Tutorials: 20 Marks
Attendance: 05 Marks
Total: 25 Marks
Assessment
Internal
Assessment consists of two tests which should be conducted at proper
intervals.
End Semester theory examination
Question paper will comprise of 6 questions each carrying 20 questions.
Total 4 questions need to be solved
Question no.1 will be compulsory based on entire syllabus wherein sub
questions can be asked.
Remaining questions will be randomly selected from all the modules
Weightage of marks should be proportional to number of hou rs assigned to
each module
Text Books
1. Operations Research; Hamdy A. Taha; Eighth Edition; Prentice Hall India
References
1.Hillier and Lieberman; Introduction to Operations Research
Page 107
University of Mumbai B. E. (Biotechnology) Rev 2016 Page 107
Course Code Course/Subject Name Credits
BTE7033 Department Elective -III: Project Management 4
Pre-requisites:
Knowledge of Fundamentals of project
Course Objectives:
To get acquainted with various aspects of project management.
To study different scheduling and planning techniques used in the industry.
To study various applications of inventory and project management with
respect to the Bioprocess Industry.
To study Life -cycle of the project.
To develop and strengthen entrepreneurial quality in students.
To impart basic entrepreneurial skills and understand ings to run a business
efficiently and effectively.
Course outcomes:
At the end of the course, learners should be able to;
Describe the fundamental concepts in Project management
Analyse the various scheduling and planning techniques
Understand and apply suitable strategy for any specific project
Apply project management principles in business situations to optimize
resource utilization and time.
Module Contents Contact
Hours
01 Introduction to Project Management:
Management, Definition, Goal, Lifecycles.
Project Selection Methods. Project Portfolio Process,
Project Formulation.
Project Manager – Roles, Responsibilities and Selection,
Project Teams. 08
02 Planning and Budgeting:
The Planning Process – Work Break down Structure
Role of Multidisciplinary teams.
Budget the Project – Methods.
Estimating and Improvement.
Budget uncertainty and risk management. 09
03 Scheduling and Resource allocation:
GANTT Chart, PERT & CPM Netw orks, GERT, Crashing
Project Uncertainty and Risk Management – Simulation –
Gantt Charts.
Algorithms for solving sequencing problems – Processing
of N jobs through K machines,
Assignments and transportation algorithms -Expediting a
project –Resource loadi ng and leveling. Allocating scarce
resources –Goldratt’s Critical Chain. 09
04 Project control and conclusion:
The Plan -Monitor -Control cycle – Data Collecting and
reporting 09
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University of Mumbai B. E. (Biotechnology) Rev 2016 Page 108
Project Control – Designing the control system.
Project Evaluation,
Auditing and Termination.
Term Work
Term work shall consist of minimum eight tutorials from entire syllabus which
are to be given at regular intervals Batch wise.
Tutorials: 20 Marks
Attendance: 05 Marks
Total: 25 Marks
Assessment
Internal
Assessment consists of two tests which should be conducted at proper
intervals.
End Semester theory examination
Question paper will comprise of 6 questions each carrying 20 questions.
Total 4 questions need to be solved
Question no.1 will be compulsory ba sed on entire syllabus wherein sub
questions can be asked.
Remaining questions will be randomly selected from all the modules
Weightage of marks should be proportional to number of hours assigned to
each module
References
1. John M. Nicholas, “Project management for business/Technology”, Pearson
2. Uddesh Kohli, K.K Chitkara, “Project Management Handbook”, Tata
McGraw Hill
3. Samuel J. Mantel et al, “Project management”, Wiley India
4. S. Choudhury, “Project Management”, Tata McGraw Hill
5. P K Joy, “Total Project Management –The Indian context”, Macmillan
6. Hisrich, Entrepreneurship, Tata McGraw Hill, New Delhi, 2001
7. S.S. Khanka, Entrepreneurial Development, S. Chand and Company
Limited
Page 109
University of Mumbai B. E. (Biotechnology) Rev 2016 Page 109
Course Code Course/Subject Name Credits
BTE7034 Department Elective -III: Nanotechnology 4
Pre-requisites:
Knowledge of Biophysics, Biochemistry, Molecular Biology, Immunology
and Analytical Methods in Biotechnology
Course Objectives:
To develop the skills of the student in the area of Nanotechnology and its
application.
Tofamiliarizestudentwithdi fferenttechniquesforsynthesizingandcharacterizin
gof various nanoparticles.
Course outcomes:
Students will have an in depth understanding of the components of
Nanotechnology and the instruments used in Nanotechnology.
Students will be able to apply the concepts of Nanotechnology in various
fields.
Module Contents Contact
Hours
01 Basics and Scale of Nanotechnology:
Introduction, Scientific revolutions, Time and length scale in
structures, Definition of a nanosystem, Dimensionality and
size dependent phenomena, Surface to volume ratio -Fraction
of surface atoms, surface energy and surface stress, surfaced
efects, Properties at nanoscale (optical, mechanical, electronic
and magnetic) 06
02 Different Classes of Nanomaterials:
Classification based on dimensionality, Quantum Dots, Wells
and Wires, Carbon -based nano materials (buckyballs,
nanotubes, graphene), Metal based nanomaterials (nanogold,
nanosilver and metal oxides), Nanocomposites,
Nanopolymers, Nanoglasses, Nano ceramics, Biological
nanomaterials 07
03 DNA and Protein based Nanostructures:
DNA -gold particle conjugates, Polymer nanocontainers,
Nanopores and nanomembranes for biochemical sensing,
Micro and nanofluidic devices in biological studies, Peptide
nanotubes and their applications in electronics, antibacterial
agents; protein self -assembly, nanochips, nanopolymers 07
04 Nano -bioanalytics:
Luminescent Quantum Dots for B iological Labeling,
Nanoparticle Molecular Labels
Surface Biology: Analysis of Biomolecular Structure by
Atomic Force Microscopy and Molecular Pulling -Force
Spectroscopy, Biofunctionalized Nanoparticles for Surface
Enhanced Raman Scattering and Surface Plasmon Resonance,
Bioconjugated Silica Nanoparticles for Bioanalytical
Applications 06
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05 Nanotechnology in Food, Medicine and Health Sciences:
Nanocomposites for food packaging, nanomaterials in
cosmetics, Regenerative medicine - Nanostructured collagen
mimics in tissue engineering, synthesis of nanodrugs,
polymeric nanoparticle for Drug and gene delivery, Micelles
for drug delivery, Nanotechnology in cancer research,
Preparation of nanobiomaterials -Polymeric scaffolds collagen,
Elastins, Mucopolysacchari des, proteoglycans, cellulose and
derivates, Dextrans, Alginates, Pectins, Chitin Toxicity and
Environmental Risks of Nanomaterial 09
Term Work
Term work shall consist of minimum eight tutorials from entire syllabus which
are to be given at regular intervals Batch wise.
Tutorials: 20 Marks
Attendance: 05 Marks
Total: 25 Marks
Assessment
Internal
Assessment consists of two tests which should be conducted at proper
intervals.
End Semester theory examination
Question paper will comprise of 6 questions each carrying 20 questions.
Total 4 questions need to be solved
Question no.1 will be compulsory based on entire syllabus wherein sub
questions can be asked.
Remaining questions will be randomly selected from all the modules
Weightage of marks should be proportional to number of hours assigned to
each module
References
1. Pradeep T., A textbook of nanoscience and nanotechnology, Tata McGraw
Hill Education Pvt. Ltd., 2012.
2. Hari Singh Nalwa, Nanostructured Material and Nanotechnology, Academic
Press, 2002
3. Niemeyer C. M., Bionanotechnology: Concepts, Application and
Perspectives Wiley VCH, 2006
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University of Mumbai B. E. (Biotechnology) Rev 2016 Page 111
Course Code Course Name Credits
ILO7011 Institute Level Optional Subject I - Product Life Cycle
Management 03
Objectives:
To familiarize the students with the need, benefits and components of PLM
To acquaint students with Product Data Management & PLM strategies
To give insights into new product development program and guidelines for
designing and developing a product
To famili arize the students with Virtual Product Development
Outcomes:
Learner will be able to…
Gain knowledge about phases of PLM, PLM strategies and methodology for
PLM feasibility study and PDM implementation.
Illustrate various approaches and techniques for designing and developing
products.
Apply product engineering guidelines / thumb rules in designing products for
moulding, machining, sheet metal working etc.
Acquire knowledge in applying virtual product development tools for
components, machining and ma nufacturing plant
Module Detailed Contents Contact
Hours
01 Introduction to Product Lifecycle Management (PLM):Product
Lifecycle Management (PLM), Need for PLM, Product
Lifecycle Phases, Opportunities of Globalization, Pre -PLM
Environment, PLM Paradigm, Importance & Benefits of PLM,
Widespread Impact of PLM, Focus and Application, A PLM
Project, Starting the PLM Initiative, PLM Applications
PLM Strategies: Industrial strategies, Strategy elements, its
identification, selection and implementation , Developing PLM
Vision and PLM Strategy, Change management for PLM. 10
02 Product Design: Product Design and Development Process,
Engineering Design, Organization and Decomposition in
Product Design, Typologies of Design Process Models,
Reference Model, Product Design in the Context of the
Product Development Process, Relation with the Development
Process Planning Phase, Relation with the Post design
Planning Phase, Methodological Evolution in Product Design,
Concurrent Engineering, Characteristic Feature s of Concurrent
Engineering, Concurrent Engineering and Life Cycle
Approach, New Product Development (NPD) and Strategies,
Product Configuration and Variant Management, The Design
for X System, Objective Properties and Design for X Tools,
Choice of Design for X Tools and Their Use in the Design
Process. 09
03 Product Data Management (PDM):Product and Product Data, 05
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University of Mumbai B. E. (Biotechnology) Rev 2016 Page 112
PDM systems and importance, Components of PDM, Reason
for implementing a PDM system, financial justification of
PDM, barriers to PDM implementat ion.
04 Virtual Product Development Tools: For components,
machines, and manufacturing plants, 3D CAD systems and
realistic rendering techniques, Digital mock -up, Model
building, Model analysis, Modeling and simulations in Product
Design, Examples/Case studies. 05
05 Integration of Environmental Aspects in Product Design:
Sustainable Development, Design for Environment, Need for
Life Cycle Environmental Strategies, Useful Life Extension
Strategies, End -of-Life Strategies, Introduction of
Environmental Strategies into the Design Process, Life Cycle
Environmental Strategies and Considerations for Product
Design. 05
06 Life Cycle Assessment and Life Cycle Cost Analysis:
Properties, and Framework of Life Cycle Assessment, Phases
of LCA in ISO Standards, Fields of Application and
Limitations of Life Cycle Assessment, Cost Analysis and the
Life Cycle Approach, General Framework for LCCA,
Evolution of Models for Product Life Cycle Cost Analysis. 05
Assessment
Internal
Assessment consists of two tests which should be conducted at proper
intervals.
End Semester theory examination
Question paper will comprise of 6 questions each carrying 20 questions.
Total 4 questions need to be solved
Question no.1 will be compulsory based on entire syllabus wherein sub
questions can be asked.
Remaining questions will be randomly selected from all the modules
Weightage of marks should be proportional to number of hours assigned to
each module
References
1. John Stark, “Product Lifecycle Management: Paradigm for 21st Century
Product Realisation”, Springer -Verlag, 2004. ISBN: 1852338105
2. Fabio Giudice, Guido La Rosa, Antonino Risitano, “Product Design for the
environment -A life cycle approach”, Taylor & Francis 2006, ISBN:
0849327229
3. Saaksvuori Antti, Immonen Anselmie, “Product Lif e Cycle Management”,
Springer, Dreamtech, ISBN: 3540257314
4. Michael Grieve, “Product Lifecycle Management: Driving the next generation
of lean thinking”, Tata McGraw Hill, 2006, ISBN: 0070636265
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University of Mumbai B. E. (Biotechnology) Rev 2016 Page 113
Course Code Course Name Credits
ILO7012 Institute Level Optional Subject I - Reliability
Engineering 03
Objectives:
To familiarize the students with various aspects of probability theory
To acquaint the students with reliability and its concepts
To introduce the students to methods of estimating the system reliability of
simple and complex systems
To understand the various aspects of Maintainability, Availability and FMEA
procedure
Outcomes:
Learner will be able to…
Understand and apply the concept of Probability to engineering problems
Apply various reliab ility concepts to calculate different reliability parameters
Estimate the system reliability of simple and complex systems
Carry out a Failure Mode Effect and Criticality Analysis
Module Detailed Contents Contact
Hours
01 Probability theory: Probability: Standard definitions and
concepts; Conditional Probability, Baye’s Theorem.
Probability Distributions: Central tendency and Dispersion;
Binomial, Normal, Poisson, Weibull, Exponential, relations
between them and their significance.
Measures of Dispersion: Mean Median, Mode, Range, Mean
Deviation, Standard Deviation, Variance, Skewness and
Kurtosis. 08
02 Reliability Concepts: Reliability definitions, Importance of
Reliability, Quality Assurance and Reliability, Bath Tub
Curve.
Failure Data Analysis: Hazard rate, failure density, Failure
Rate, Mean Time To Failure (MTTF), MTBF, Reliability
Functions.
Reliability Hazard Models: Constant Failure Rate, Linearly
increasing, Time Dependent Failure Rate, Weibull Model.
Distribution functions and reliability analysis. 08
03 System Reliability: System Configurations: Series, parallel,
mixed configuration, k out of n structure, Complex systems. 05
04 Reliability Improvement: Redundancy Techniques: Element
redundancy, Unit redundancy, Standby redundancies. Mark ov
analysis.
System Reliability Analysis – Enumeration method, Cut -set
method, Success
Path method, Decomposition method. 08
05 Maintainability and Availability: System downtime, Design
for Maintainability: Maintenance requirements, Design
methods: Fault Isolation and self -diagnostics, Parts 05
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University of Mumbai B. E. (Biotechnology) Rev 2016 Page 114
standardization and Interchangeability, Modularization and
Accessibility, Repair Vs Replace ment.
Availability – qualitative aspects.
06 Failure Mode, Effects and Criticality Analysis: Failure mode
effects analysis, severity/criticality analysis, FMECA
examples. Fault tree construction, basic symbols, development
of functional reliability blo ck diagram, Fau1t tree analysis and
Event tree Analysis
05
Assessment
Internal
Assessment consists of two tests which should be conducted at proper
intervals.
End Semester theory examination
Question paper will comprise of 6 questions each carrying 20 questions.
Total 4 questions need to be solved
Question no.1 will be compulsory based on entire syllabus wherein sub
questions can be asked.
Remaining questions will be randomly selected from all the modules
Weightage of marks should be proportional to number of hours assigned to
each module
References
1. L.S. Srinath, “Reliability Engineering”, Affiliated East -Wast Press (P) Ltd.,
1985.
2. Charles E. Ebeling, “Reliability and Maintainability Engineering”, Tata
McGraw Hill.
3. B.S. Dhillion, C. Singh, “Engineerin g Reliability”, John Wiley & Sons, 1980.
4. P.D.T. Conor, “Practical Reliability Engg. ” John Wiley & Sons, 1985.
5. K.C. Kapur, L.R. Lamberson, “Reliability in Engineering Design”, John Wiley
& Sons.
6. Murray R. Spiegel, “Probability and Statistics”, Tata McGraw -Hill Publishing
Co. Ltd.
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University of Mumbai B. E. (Biotechnology) Rev 2016 Page 115
Course Code Course Name Credits
ILO7013 Institute Level Optional Subject I - Management
Information System 03
Objectives :
The course is blend of Management and Technical field.
Discuss the roles played by information technology in today’s business and
define various technology architectures on which information systems are
built
Define and analyze typical functional information systems and identify how
they meet the needs of the firm to deliver efficiency and competiti ve
advantage
Identify the basic steps in systems development
Outcomes:
Learner will be able to…
Explain how information systems Transform Business
Identify the impact information systems have on an organization
Describe IT infrastructure and its components and its current trends
Understand the principal tools and technologies for accessing information
from databases to improve business performance and decision making
Identify the types of systems used for enterprise -wide knowledge management
and how they provide value for businesses
Module Detailed Contents Contact
Hours
01 Introduction To Information Systems (IS): Computer Based
Information Systems, Impact of IT on organizations, and
Importance of IS to Society. Organizational Strategy,
Competitive Advan tages and IS. 4
02 Data and Knowledge Management: Database Approach, Big
Data, Data warehouse and Data Marts, Knowledge
Management.
Business intelligence (BI): Managers and Decision Making,
BI for Data analysis and Presenting Results 7
03 Ethical issues and Privacy: Information Security. Threat to
IS, and Security Controls 7
04 Social Computing (SC): Web 2.0 and 3.0, SC in business -
shopping, Marketing, Operational and Analytic CRM, E -
business and E -commerce – B2B B2C. Mobile commerce. 7
05 Computer Networks Wired and Wireless technology,
Pervasive computing, Cloud computing model. 6
06 Information System within Organization: Transaction
Processing Systems, Functional Area Information System,
ERP and ERP support of Business Process.
Acquirin g Information Systems and Applications: Various
System development life cycle models. 8
Assessment
Internal
Page 116
University of Mumbai B. E. (Biotechnology) Rev 2016 Page 116
Assessment consists of two tests which should be conducted at proper
intervals.
End Semester theory examination
Question paper will comprise of 6 questions each carrying 20 questions.
Total 4 questions need to be solved
Question no.1 will be compulsory based on entire syllabus wherein sub
questions can be asked.
Remaining questions will be randomly selected from all the modules
Weightage of marks s hould be proportional to number of hours assigned to
each module
References
1. Kelly Rainer, Brad Prince, Management Information Systems, Wiley
2. K.C. Laudon and J.P. Laudon, Management Information Systems: Managing
the Digital Firm, 10th Ed., Prentice Hall, 20 07.
3. D. Boddy, A. Boonstra, Managing Information Systems: Strategy and
Organization, Prentice Hall, 2008
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University of Mumbai B. E. (Biotechnology) Rev 2016 Page 117
Course Code Course Name Credits
ILO7014 Institute Level Optional Subject I - Design of
Experiments 03
Objectives:
To understand the issues and principles of Design of Experiments (DOE)
To list the guidelines for designing experiments
To become familiar with methodologies that can be used in conjunction with
experimental designs for robustness and optimization
Outcomes:
Learner will be able to…
Plan data collection, to turn data into information and to make decisions that
lead to appropriate action
Apply the methods taught to real life situations
Plan, analyze, and interpret the results of experiments
Module Detailed Contents Contact
Hours
01 Introduction
1.1 Strategy of Experimentation
1.2 Typical Applications of Experimental Design
1.3 Guidelines for Designing Experiments
1.4 Response Surface Methodology 06
02 Fitting Regression Models
2.1 Linear Regression Models
2.2 Estimation of the Parameters in Linear Regression Models
2.3 Hypothesis Testing in Multiple Regression
2.4 Confidence Intervals in Multiple Regression
2.5 Prediction of new response observation
2.6 Regression model diagnostics
2.7 Testing for lack of fit 08
03 Two-Level Fa ctorial Designs and Analysis
3.1 The 22 Design
3.2 The 23 Design
3.3 The General2k Design
3.4 A Single Replicate of the 2k Design
3.5 The Addition of Center Points to the 2k Design,
3.6 Blocking in the 2k Factorial Design
3.7 Split -Plot Designs 07
04 Two-Level Fractional Factorial Designs and Analysis
4.1 The One -Half Fraction of the 2k Design
4.2 The One -Quarter Fraction of the 2k Design
4.3 The General 2k -p Fractional Factorial Design
4.4 Resolution III Designs
4.5 Resolution IV and V Designs
4.6 Fractional Factorial Split -Plot Designs 07
05 Conducting Tests
5.1 Testing Logistics 07
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University of Mumbai B. E. (Biotechnology) Rev 2016 Page 118
5.2 Statistical aspects of conducting tests
5.3 Characteristics of good and bad data sets
5.4 Example experiments
5.5 Attribute Vs Variable data sets
06 Taguchi Appro ach
6.1 Crossed Array Designs and Signal -to-Noise Ratios
6.2 Analysis Methods
6.3 Robust design examples 04
Assessment
Internal
Assessment consists of two tests which should be conducted at proper
intervals.
End Semester theory examination
Question paper will comprise of 6 questions each carrying 20 questions.
Total 4 questions need to be solved
Question no.1 will be compulsory based on entire syllabus wherein sub
questions can be asked.
Remaining questions will be randomly selected from all the modules
Weightage of marks should be proportional to number of hours assigned to
each module
References
1. Raymond H. Mayers, Douglas C. Montgomery, Christine M. Anderson -Cook,
Response Surface Methodology: Process and Product Optimization using
Designed Experimen t, 3rd edition, John Wiley & Sons, New York, 2001
2. D.C. Montgomery, Design and Analysis of Experiments, 5th edition, John
Wiley & Sons, New York, 2001
3. George E P Box, J Stuart Hunter, William G Hunter, Statics for
Experimenters: Design, Innovation and Disco very, 2nd Ed. Wiley
4. W J Dimond, Practical Experiment Designs for Engineers and Scientists, John
Wiley and Sons Inc. ISBN: 0 -471-39054 -2
5. Design and Analysis of Experiments (Springer text in Statistics), Springer by
A.M. Dean, and D. T. Voss
6. Phillip J Ross, “Taguchi Technique for Quality Engineering,” McGraw Hill
7. Madhav S Phadke, “ Quality Engineering using Robust Design,” Prentice Hall
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Course Code Course Name Credits
ILO7015 Institute Level Optional Subject I - Operations
Research 03
Objectives:
Formulate a real -world problem as a mathematical programming model.
Understand the mathematical tools that are needed to solve optimization
problems.
Use mathematical software to solve the proposed models.
Outcomes:
Learner will be able to…
Understand th e theoretical workings of the simplex method, the relationship
between a linear program and its dual, including strong duality and
complementary slackness.
Perform sensitivity analysis to determine the direction and magnitude of
change of a model’s optimal solution as the data change.
Solve specialized linear programming problems like the transportation and
assignment problems; solve network models like the shortest path, minimum
spanning tree, and maximum flow problems.
Understand the applications of inte ger programming and a queuing model
and compute important performance measures
Module Detailed Contents Contact
Hours
01 Introduction to Operations Research: Introduction, ,
Structure of the Mathematical Model, Limitations of
Operations Research
Linear Programming: Introduction, Linear Programming
Problem, Requirements of LPP, Mathematical Formulation
of LPP, Graphical method, Simplex Method Penalty Cost
Method or Big M -method, Two Phase Method, Revised
simplex method, Duality, Primal – Dual construc tion,
Symmetric and Asymmetric Dual, Weak Duality Theorem,
Complimentary Slackness Theorem, Main Duality Theorem,
Dual Simplex Method, Sensitivity Analysis
Transportation Problem: Formulation, solution, unbalanced
Transportation problem. Finding basic fea sible solutions –
Northwest corner rule, least cost method and Vogel’s
approximation method. Optimality test: the stepping stone
method and MODI method.
Assignment Problem: Introduction, Mathematical
Formulation of the Problem, Hungarian Method
Algorithm, Processing of n Jobs Through Two Machines
and m Machines, Graphical Method of Two Jobs m
Machines Problem Routing Problem, Travelling Salesman
Problem
Integer Programming Problem: Introduction, Types of
Integer Programming Problems, Gomory’s cutting plane
Algorithm, Branch and Bound Technique. Introduction to 14
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University of Mumbai B. E. (Biotechnology) Rev 2016 Page 120
Decomposition algorithms.
02 Queuing models: queuing systems and structures, single
server and multi -server models, Poisson input, exponential
service, constant rate service, finite and infinite population 05
03 Simulation: Introduction, Methodology of Simulation, Basic
Concepts, Simulation Procedure, Application of Simulation
Monte -Carlo Method: Introduction, Monte -Carlo
Simulation, Applications of Simulation, Advantages of
Simulation, Limitati ons of Simulation 05
04 Dynamic programming. Characteristics of dynamic
programming. Dynamic programming approach for Priority
Management employment smoothening, capital budgeting,
Stage Coach/Shortest Path, cargo loading and Reliability
problems. 05
05 Game Theory. Competitive games, rectangular game, saddle
point, minimax (maximin) method of optimal strategies,
value of the game. Solution of games with saddle points,
dominance principle. Rectangular games without saddle
point – mixed strategy for 2 X 2 games. 05
06 Inventory Models: Classical EOQ Models, EOQ Model with
Price Breaks, EOQ with Shortage, Probabilistic EOQ Model, 05
Assessment
Internal
Assessment consists of two tests which should be conducted at proper
intervals.
End Semester theory examination
Question paper will comprise of 6 questions each carrying 20 questions.
Total 4 questions need to be solved
Question no.1 will be compulsory based on entire syllabus wherein sub
questions can be asked.
Remaining questions will be randomly sele cted from all the modules
Weightage of marks should be proportional to number of hours assigned to
each module
References
1. Taha, H.A. "Operations Research - An Introduction", Prentice Hall, (7th
Edition), 2002.
2. Ravindran, A, Phillips, D. T and Solberg, J. J . "Operations Research:
Principles and Practice", John Willey and Sons, 2nd Edition, 2009.
3. Hiller, F. S. and Liebermann, G. J. "Introduction to Operations Research",
Tata McGraw Hill, 2002.
4. Operations Research, S. D. Sharma, Kedar Nath Ram Nath -Meerut.
5. Operations Research, Kanti Swarup, P. K. Gupta and Man Mohan, Sultan
Chand & Sons.
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Course Code Course Name Credits
ILO7016 Institute Level Optional Subject I - Cyber Security
and Laws 03
Objectives:
To understand and identify different types cybercrime and cyber law
To recognized Indian IT Act 2008 and its latest amendments
To learn various types of security standards compliances
Outcomes:
Learner will be able to…
Understand the concept of cybercrime and its effect on outside world
Interpret and apply IT law in various legal issues
Distinguish different aspects of cyber law
Apply Information Security Standards compliance during software design and
development
Module Detailed Contents Contact
Hours
1 Introduction to Cybercrime: Cybercrime definition and origins
of the world, Cybercrime and information security,
Classifications of cybercrime, Cybercrime and the
Indian ITA 2000, A global Perspective on cybercrimes. 4
2 Cyber offenses & Cybercrime: How criminal plan the attacks,
Social Engg, Cyber stalking, Cyber café and Cybercrimes,
Botnets, Attack vector, Cloud computing, Proliferation of
Mobile and Wireless Devices, Trends in Mobility, Credit Card
Frauds in
Mobile and Wireless Computing Era, Security Challenges
Posed by Mobile Devices, Registry Settings for Mobile
Devices, Authentication Service Security, Attacks on
Mobile/Cell Phones, Mobile Devices: Security Implications for
Organizations, Organizational Measures for Handling Mobile,
Devices -Related Security Issues, Organizational Security
Policies and Measures in Mobile Computing Era, Laptops 9
3 Tools and Methods Used in Cyberline
Phishing, Password Cracking, Keyloggers and Spywares, Virus
and Worms, Steganography, DoS and DDoS Attacks, SQL
Injection, Buffer Over Flow, Attacks on Wireless Ne tworks,
Phishing, Identity Theft (ID Theft) 6
4 The Concept of Cyberspace
E-Commerce , The Contract Aspects in Cyber Law ,The
Security Aspect of Cyber Law ,The Intellectual Property
Aspect in Cyber Law
, The Evidence Aspect in Cyber Law , The Criminal A spect in
Cyber Law, Global Trends in Cyber Law , Legal Framework
for Electronic Data Interchange Law Relating to Electronic
Banking, The Need for an Indian Cyber Law 8
5 Indian IT Act.
Cyber Crime and Criminal Justice: Penalties, Adjudication and 6
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Appeals Under the IT Act, 2000, IT Act. 2008 and its
Amendments
6 Information Security Standard compliances
SOX, GLBA, HIPAA, ISO, FISMA, NERC, PCI. 6
Assessment
Internal
Assessment consists of two tests which should be conducted at proper
intervals.
End Semester theory examination
Question paper will comprise of 6 questions each carrying 20 questions.
Total 4 questions need to be solved
Question no.1 will be compulsory based on entire syllabus wherein sub
questions can be asked.
Remaining questions will be randomly selected from all the modules
Weightage of marks should be proportional to number of hours assigned to
each module
References
1. Nina Godbole, Sunit Belapure, Cyber Security, Wiley India, New Delhi
2. The Indian Cyber Law by Suresh T. Vishwanathan; Bharat Law House New
Delhi
3. The Information technology Act, 2000; Bare Act - Professional Book
Publishers, New Delhi.
4. Cyber Law & Cyber Crimes By Advocate Prashant Mali; Snow White
Publications, Mumbai
5. Nina Godbole, Information Systems Security , Wiley India, New Delhi
6. Kennetch J. Knapp, Cyber Security &Global Information Assurance
Information Science Publishing.
7. William Stallings, Cryptography and Network Security, Pearson Publication
8. Websites for more information is available on : The Informa tion Technology
ACT, 2008 - TIFR : https://www.tifrh.res.in
9. Website for more information , A Compliance Primer for IT professional :
https://www.sans.org/reading -room/whitepapers/compliance/compliance -
primer -professionals -33538
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Course Code Course Name Credits
ILO7017 Institute Level Optional Subject I - Disaster
Management and Mitigation Measures 03
Objectives:
To understand physics and various types of disaster occurring around the
world
To identify extent and damaging capacity of a disaster
To study and understand the means of losses and methods to overcome
/minimize it.
To understand role of individual and various organization during and after
disaster
To understand application of GIS in the field of disaster management
To understand the emergency government response structures before, during
and after disaster
Outcomes:
Learner will be able to…
Get to know natural as well as manmade disaster and their extent and possible
effects on the economy.
Plan of national importance structures base d upon the previous history.
Get acquainted with government policies, acts and various organizational
structures associated with an emergency.
Get to know the simple do’s and don’ts in such extreme events and act
accordingly.
Module Detailed Contents Cont act
Hours
01 Introduction:
Definition of Disaster, hazard, global and Indian scenario,
general perspective, importance of study in human life, Direct
and indirect effects of disasters, long term effects of disasters.
Introduction to global warming and climate change. 03
02 Natural Disaster and Manmade disasters:
Natural Disaster: Meaning and nature of natural disaster,
Flood, Flash flood, drought, cloud burst, Earthquake,
Landslides, Avalanches, Volcanic eruptions, Mudflow,
Cyclone, Storm, Storm Surge, climate change, global
warming, sea level rise, ozone depletion
Manmade Disasters: Chemical, Industrial, Nuclear and Fire
Hazards. Role of growing population and subsequent
industrialization, urbanization and changing lifestyle of human
beings in frequent occurrences of manmade d isasters. 09
03 Disaster Management, Policy and Administration:
Disaster management: meaning, concept, importance, objective
of disaster management policy, disaster risks in India,
Paradigm shift in disaster management.
Policy and administration: Importance and principles of
disaster management policies, command and co -ordination of
in disaster management, rescue operations -how to start with 06
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University of Mumbai B. E. (Biotechnology) Rev 2016 Page 124
and how to proceed in due course of time, study of flowchart
showing the entire p rocess.
04 Institutional Framework for Disaster Management in India:
Importance of public awareness, Preparation and execution of
emergency management programme. Scope and
responsibilities of National Institute of Disaster Management
(NIDM) and National disaster management authority (NDMA)
in India. Methods and measures to avoid disasters,
Management of casualties, set up of emergency facilities,
importance of effective communication amongst different
agencies in such situations.
Use of Internet and softwares for effective disaster
management. Applications of GIS, Remote sensing and GPS in
this regard. 06
05 Financing Relief Measures:
Ways to raise finance for relief expenditure, role of
government agencies and NGO’s in this process, Legal aspects
related to finance raising as well as overall management of
disasters. Various NGO’s and the works they have carried out
in the past on the occurrence of various disasters, Ways to
approach these teams. International relief aid agencies and their
role in extreme events. 09
06 Preventive and Mitigation Measures:
Pre-disaster, during disaster and post -disaster measures in
some events in general structural mapping: Risk mapping,
assessment and analysis, sea walls and embankments, Bio
shield, shelters, early warning and communication
Non Structural Mitigation: Community based disaster
preparedness, risk transfer and risk financing, capacity
development and training, awareness and education,
contingency plans. Do’s and don’ts in case of disasters and
effective implementation of relief aids. 06
Assessment
Internal
Assessment consists of two tests which should be conducted at proper
intervals.
End Semester theory examination
Question paper will comprise of 6 questions each carrying 20 questions.
Total 4 questions need to be solved
Question no.1 will be compulsory based on entire syllabus wherein sub
questions can be asked.
Remaining questions will be randomly selected from all the modules
Weightage of marks should be proportional to number of hou rs assigned to
each module
References
1. ‘Disaster Management’ by Harsh K.Gupta, Universities Press Publications.
2. ‘Disaster Management: An Appraisal of Institutional Mechanisms in India’ by
O.S. Dagur, published by Centre for land warfare studies, New Delhi, 2011.
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University of Mumbai B. E. (Biotechnology) Rev 2016 Page 125
3. ‘Introduction to International Disaster Management’ by Damon Copolla,
Butterworth Heinemann Elseveir Publications.
4. ‘Disaster Management Handbook’ by Jack Pinkowski, CRC Press Taylor and
Francis group.
5. ‘Disaster management & rehabilitation’ by Rajdeep , Dasgupta, Mittal
Publications, New Delhi.
6. ‘Natural Hazards and Disaster Management, Vulnerability and Mitigation – R
B Singh, Rawat Publications
7. Concepts and Techniques of GIS –C.P.Lo Albert, K.W. Yonng – Prentice Hall
(India) Publications.
8. (Learners ar e expected to refer reports published at national and International
level and updated information available on authentic web sites)
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University of Mumbai B. E. (Biotechnology) Rev 2016 Page 126
Course Code Course Name Credits
ILO7018 Institute Level Optional Subject I - Energy Audit and
Management 03
Objectives:
To understand the importance energy security for sustainable development and
the fundamentals of energy conservation.
To introduce performance evaluation criteria of various electrical and thermal
installations to fac ilitate the energy management
To relate the data collected during performance evaluation of systems for
identification of energy saving opportunities.
Outcomes:
Learner will be able to…
To identify and describe present state of energy security and its importance.
To identify and describe the basic principles and methodologies adopted in
energy audit of an utility.
To describe the energy performance evaluation of some common electrical
installations and identify the energy saving opportunities.
To descr ibe the energy performance evaluation of some common thermal
installations and identify the energy saving opportunities
To analyze the data collected during performance evaluation and recommend
energy saving measures
Module Detailed Contents Contact
Hours
1 Energy Scenario:
Present Energy Scenario, Energy Pricing, Energy Sector
Reforms, Energy Security, Energy Conservation and its
Importance, Energy Conservation Act -2001 and its Features.
Basics of Energy and its various forms, Material and Energy
balance 04
2 Energy Audit Principles:
Definition, Energy audit - need, Types of energy audit, Energy
management (audit) approach -understanding energy costs,
Bench marking, Energy performance, Matching energy use to
requirement, Maximizing system efficiencies, Optimizing the
input e nergy requirements, Fuel and energy substitution.
Elements of monitoring& targeting; Energy audit Instruments;
Data and information -analysis.
Financial analysis techniques: Simple payback period, NPV,
Return on investment (ROI), Internal rate of return (I RR) 08
3 Energy Management and Energy Conservation in Electrical
System:
Electricity billing, Electrical load management and maximum
demand Control; Power factor improvement, Energy efficient
equipments and appliances, star ratings.
Energy efficiency m easures in lighting system, Lighting
control: Occupancy sensors, daylight integration, and use of
intelligent controllers. 10
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University of Mumbai B. E. (Biotechnology) Rev 2016 Page 127
Energy conservation opportunities in: water pumps, industrial
drives, induction motors, motor retrofitting, soft starters,
variable speed drives.
4 Energy Management and Energy Conservation in Thermal
Systems:
Review of different thermal loads; Energy conservation
opportunities in: Steam distribution system, Assessment of
steam distribution losses, Steam leakages, Steam trapping,
Condensate and flash steam recovery system.
General fuel economy measures in Boilers and furnaces, Waste
heat recovery, use of insulation - types and application. HVAC
system: Coefficient of performance, Capacity, factors affecting
Refrigeration and Air Co nditioning system performance and
savings opportunities. 10
5 Energy Performance Assessment:
On site Performance evaluation techniques, Case studies based
on: Motors and variable speed drive, pumps, HVAC system
calculations; Lighting System: Installed Load Efficacy Ratio
(ILER) method, Financial Analysis. 04
6 Energy conservation in Buildings:
Energy Conservation Building Codes (ECBC): Green
Building, LEED rating, Application of Non -Conventional and
Renewable Energy Sources 03
Assessment
Internal
Assessment consists of two tests which should be conducted at proper
intervals.
End Semester theory examination
Question paper will comprise of 6 questions each carrying 20 questions.
Total 4 questions need to be solved
Question no.1 will be compulsory based on entire syllabus wherein sub
questions can be asked.
Remaining questions will be randomly selected from all the modules
Weightage of marks should be proportional to number of hours assigned to
each module
References
1. Handbook of Electrical Installation Practice, Geofry Stokes, Blackwell
Science
2. Designing with light: Lighting Handbook, By Anil Va lia, Lighting System
3. Energy Management Handbook, By W.C. Turner, John Wiley and Sons
4. Handbook on Energy Audits and Management, edited by A. K. Tyagi, Tata
Energy Research Institute (TERI).
5. Energy Management Principles, C.B. Smith, Pergamon Press
6. Energy Conservation Guidebook, Dale R. Patrick, S. Fardo, Ray E.
Richardson, Fairmont Press
7. Handbook of Energy Audits, Albert Thumann, W. J. Younger, T. Niehus,
CRC Press
8. www.energymanagertraining.com
9. www.bee -india.nic.in
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University of Mumbai B. E. (Biotechnology) Rev 2016 Page 128
Course Code Course Name Credits
ILO7019 Institute Level Optional Subject I - Development
Engineering 03
Pre-requisite:
Interest in societal development.
Course Objective:
To understand the characteristics of rural Society and the Scope and Nature
and Constraints of rural Development.
To study Implications of 73rd CAA on Planning, Development and
Governance of Rural Areas
The objective of the course is an exploration of human values, which go into
making a ‘good’ human being, a ‘good’ professional, a ‘good’ society and a
‘good life’. Th e context is the work life and the personal life of modern Indian
professionals.
To understand the Nature and Type of Human Values relevant to Planning
Institutions.
Course Outcome:
Students will be able to apply knowledge for Rural Development.
Students will be able to apply knowledge for Management Issues.
Students will be able to apply knowledge for Initiatives and Strategies
Students will be able to develop acumen for higher education and research.
Students will master the art of working in group of different nature.
Students will develop confidence to take up rural project activities
independently.
Module Contents Contact
Hours
1 Introduction to Rural Development Meaning, nature and scope
of development; Nature of rural society in India; Hierarchy of
settlements; Social, economic and ecological constraints for
rural development. 04
2 Roots of Rural Development in India Rural reconstruction and
Sarvodaya programme before independence; Impact of
voluntary effort and Sarvodaya Movement on rural
development; Constitutional direction, directive principles;
Panchayati Raj - beginning of planning and community
development; National extension services. 04
3 Post-Independence rural Development Balwant Rai Mehta
Committee - three tier system of r ural local Government; Need
and scope for people’s participation and Panchayati Raj;
Ashok Mehta Committee - linkage between Panchayati Raj,
participation and rural development. 04
4 Rural Development Initiatives in Five Year Plans Five Year
Plans and Rur al Development; Planning process at National,
State, Regional and District levels; Planning, development,
implementing and monitoring organizations and agencies; 06
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University of Mumbai B. E. (Biotechnology) Rev 2016 Page 129
Urban and rural interface - integrated approach and local plans;
Development initiatives and t heir convergence; Special
component plan and sub -plan for the weaker section; Micro -
eco zones; Data base for local planning; Need for decentralized
planning; Sustainable rural development.
5 Post 73rd Amendment Scenario 73rd Constitution Amendment
Act, including - XI schedule, devolution of powers, functions
and finance; Panchayati Raj institutions - organizational
linkages; Recent changes in rural local planning; Gram Sabha -
revitalized Panchayati Raj; Institutionalization; resource
mapping, resource mobilization including social mobilization;
Information Technology and rural planning; Need for further
amendments. 04
6 Values and Science and Technology Material development and
its values; the challenge of science and technology; Values in
planning profession, research and education. 04
7 Types of Values Psychological values — integrated
personality; mental health; Societal values — the modern
search for a good society; justice, democracy, rule of law,
values in the Indian constitution; Aesthetic va lues —
perception and enjoyment of beauty; Moral and ethical values;
nature of moral judgment; Spiritual values; different concepts;
secular spirituality; Relative and absolute values; Human
values — humanism and human values; human rights; human
values as freedom, creativity, love and wisdom. 06
8 Ethics Canons of ethics; ethics of virtue; ethics of duty; ethics
of responsibility; Work ethics; Professional ethics; Ethics in
planning profession, research and education 04
Assessment
Internal
Assessment consists of two tests which should be conducted at proper
intervals.
End Semester theory examination
Question paper will comprise of 6 questions each carrying 20 questions.
Total 4 questions need to be solved
Question no.1 will be compulsory based on enti re syllabus wherein sub
questions can be asked.
Remaining questions will be randomly selected from all the modules
Weightage of marks should be proportional to number of hours assigned to
each module
Reference
1. ITPI, Village Planning and Rural Development, ITPI, New Delhi
2. Thooyavan, K.R. Human Settlements: A 2005 MA Publication, Chennai
3. GoI, Constitution (73rdGoI, New Delhi Amendment) Act, GoI, New Delhi
4. Planning Commission, Five Year Plans, Planning Commission
5. Planning Commission, Manual of Integrated Di strict Planning, 2006,
Planning Commission New Delhi
6. Planning Guide to Beginners
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University of Mumbai B. E. (Biotechnology) Rev 2016 Page 130
7. Weaver, R.C., The Urban Complex, Doubleday.
8. Farmer, W.P. et al, Ethics in Planning, American Planning Association,
Washington.
9. How, E., Normative Ethics in Planning, Journal of Planning Literature, Vol.5,
No.2, pp. 123 -150.
10. Watson, V. , Conflicting Rationalities: -- Implications for Planning Theory
and Ethics, Planning Theory and Practice, Vol. 4, No.4, pp.395 – 407
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University of Mumbai B. E. (Biotechnology) Rev 2016 Page 131
Course Code Course Name Credits
BTP701 Project -A 03
Guidelines:
Project groups: Students can form groups with not more than 3 (th ree).
Students should spend considerable time in applying all the concepts studied,
into the Project, hence, eight hours each are allotted in project A and B to the
students.
Students are advised to take up industrial/ experimental/ simulation and/or
optimization based topics for their project
Students should report their guides weekly with their work.
Exam Guidelines
Term Work - 100 Marks:
Presentation – 50 Marks
Report -50 Marks
Oral – 50 Marks
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University of Mumbai B. E. (Biotechnology) Rev 2016 Page 132
Course Code Course/Subject Name Credits
BTL701 Lab IV 1.5
Concepts for experiments:
A minimum of 10 experiments must be performed based on the following concepts:
Viscometer
Cell disruption
Conventional filtration
Distribution coe fficient in Liq -liq extraction
Solid -liquid extraction of natural product and subsequent purification
Leaching
Protein precipitation by various methods and its rec overy
Separation of Plant Pigments using Column Chromatography
Steam Distillation
Simple Distillation
Vacuum Filtration
Extraction of Phytochemicals using different extraction methods
Practical Examination
Duration for practical examination would be the same as assigned to the
respective lab per week.
A student becomes eligible for practical examination after completing a
minimum of eight out of ten experiments.
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University of Mumbai B. E. (Biotechnology) Rev 2016 Page 133
Course Code Course/Subject Name Credits
BTL702 Lab V 1.5
Concepts for experiments:
A minimum of 10 experiments must be performed based on the following:
Material Balance without Reaction
Material Balance with Reaction
Energy Balance equations
Solving Linear equations
Solving Non -linear algebraic equations
Parameter Estimation in kinetics
Modelling of Batch, Fed Batch and Continuous
Simulation of Batch Reactor
Simulation of Continuous Reactor
Solving Numerical integrations
Solving Algebraic equations
Solving Differential Equations
Practical Examinatio n
Duration for practical examination would be the same as assigned to the
respective lab per week.
A student becomes eligible for practical examination after completing a
minimum of eight out of ten experiments.
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University of Mumbai B. E. (Biotechnology) Rev 2016 Page 134
University of Mumbai
Program Structure for B.E. Biotechnology (Revised 2016 )
B.E Semester VIII (w.e.f 2019 -2020)
Course
code Course Name Teaching Scheme
(Contact Hours) Credits Assigned
Theory Practical Tutorial Theory Practical Tutorial Total
BTC801 Environmental Biotechnology 4 - - 4 - - 4
BTC802 Bioseperation& Downstream
Processing technology -II 4 - - 4 - - 4
BTC803 Bioprocess Plant & Equipment
design 3 - 1 3 - 1 4
BTE804X Department Elective IV 3 - 1 3 - 1 4
ILO802X Institute Level optional Subject II 3 - - 3 - - 3
BTP801 Project B - - 8 - - 6 6
BTL801 Lab - VI - 3 - - 1.5 - 1.5
BTL802 Lab - VII - 3 - - 1.5 - 1.5
Total 17 6 10 17 3 8 28
Course
code Course Name Examination Scheme
Theory Term
Work Pract/
Oral Oral Total
Internal
Assessment End
Sem
Exam Exam
Duration
(in hrs)
Test
1 Test
2 Avg
BTC801 Environmental Biotechnology 20 20 20 80 3 - - - 100
BTC802 Bioseperation& Downstream
Processing technology -II 20 20 20 80 3 - - - 100
BTC803 Bioprocess Plant & Equipment
design 20 20 20 80 3 25 - - 125
BTE804X Department Elective IV 20 20 20 80 3 25 - 125
ILO802X Institute Level optional Subject II 20 20 20 80 3 - - - 100
BTP801 Project B - - - - - 100 - 50 150
BTL801 Lab - VI - - - - 3 - 25 - 25
BTL802 Lab - VII - - - - 3 - 25 - 25
Total 100 400 - 150 50 50 750
Department Elective IV (Sem VIII)
Engineering Stream Advanced Science Stream Technology Stream
1. Non -conventional Sources of
Energy (BTE8041) 1. Total Quality Management
(BTE8042)
2. Entrepreneurship (BTE8043) 1. Advanced Bioinformatics
(BTE8044)
Institute Level Optional Subject II (Sem VIII)
1. Project Management (ILO8021) 4. Human Resource
Management (ILO8024) 7. IPR and Patenting
(ILO8027)
2. Finance Management (ILO8022) 5. Professional Ethics and CSR
(ILO8025) 8. Digital Business
Management (ILO8028)
3. Entrepreneurship Development and
Management (ILO8023) 6. Research Methodology
(ILO8026)
9. Environmental
Management (ILO8029)
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University of Mumbai B. E. (Biotechnology) Rev 2016 Page 135
Course Code Course/Subject Name Credits
BTC801 Environmental Biotechnology 4
Pre-requisites:
Knowledge of Biotechnological aspects and molecular genetics
Course Objectives:
The main objective of this course is to introduce to the students the current
biotechnological approaches and technologies in the use of microbes and/or
other organisms and their processes to improve environmental quality, clean
up contaminated environment , renew resources and generate valuable
products for human society.
Course outcomes:
Apply their knowledge of environmental science and biological systems to
improve the quality of life in individual context.
Recognize key environmental problems and to apply the operating principles
and biotic systems for remediation.
Design, improve and apply biotechnological systems and processes to meet
practical needs of different environmental problems.
Module Contents Contact
Hours
1 Introduction:
Environmental Degradation, types of environmental
degradation, factors affecting environmental degradation,
Environmental monitoring - sampling (land, air, water),
analysis - physical, chemical, biological, pollution monitoring -
bio indicators, biosensors, b iomarkers, pollution control
aspects. 03
2 Pollution control:
Atmospheric stability, atmospheric dispersion - (Gausian
plume model), air pollution control - Particulate and gaseous
control, source correction methods, natural pathways of
exchange of air pol lutants from atmosphere to earth (wet
precipitation - rain out, washout) 06
3 Water Pollution Control:
Measurement of organic and inorganic pollutants, DO
depletion, modelling of BOD reaction, problems on BOD,
Methods of waste water treatment, Microbiology and design
(activated sludge process, trickling process), Rotating
Biological contactors, Fluidized bed reactors, anaerobic
sludge digestion, Methanogenesis, methanogenic, acetogenic,
fermentative bacteria - technical process and condition, was te
water treatment using aquatic plants, heavy metal removal by
hairy roots. 07
4 Soil Pollution Control:
Bioremediation of contaminated soil, types of bioremediation,
factors affecting bioremediation, phyto fremediation, role of
genetic engineering 05
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University of Mumbai B. E. (Biotechnology) Rev 2016 Page 136
5 Solid waste management:
Types of solid waste, sources, effects, methods of collection,
disposal methods, potential methods of disposal, disposal of
hazardous waste, Biological conversion process (aerobic,
anaerobic, bioventing), biotechnology application s to
hazardous waste management 06
6 Special topics in Bioremediation technology:
Nanotechnology for bioremediation of heavy metals, sulphate
and sulphur reducing bacteria, bioremediation of petroleum
sludge using bacterial consortium and bio surfactants 04
7 Downstream Processing:
Downstream processing in biological treatment process,
effluent disposal and reuse, bio filtration of waste gas,
treatment and purification of biogas 04
8 Effuent treatment:
Need of ETP in industry, Components of ETP, general design
procedure for ETP, ETP studies of industries like dairy,
metal, food etc. 05
9 Environmental Legislations:
Water Prevention and Control Pollution Act, Water pollution
act, Air pollution and prevention act, The environment
Protection Act, Forest Co nservation Act, Municipal Solid
Waste Rules, Biomedical Waste Rules, Hazardous Wastef
Rules, Environmental Clearance, Environmental Legislation
and Pollution Control Acts in India, Central Pollution Control
Board, its functions and powers, Procedure to ope rate an
industry 02
10 Environmental Standards:
Need and Use of environmental standards, Agencies and
Bodies setting environmental standards, classification of
environmental standards, National and International
Standards for waste water 03
Assessment
Internal
Assessment consists of two tests which should be conducted at proper
intervals.
End Semester theory examination
Question paper will comprise of 6 questions each carrying 20 questions.
Total 4 questions need to be solved
Question no.1 will be compulsory based on entire syllabus wherein sub
questions can be asked.
Remaining questions will be randomly selected from all the modules
Weightage of marks should be proportional to number of hours assigned to
each module
References
1. Environmental Biotec hnology - Allen Scragg, Oxford University Press,
Second edition
2. Environmental Biotechnology, 1995 S.N. Jogdand, Himalaya Publishing
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University of Mumbai B. E. (Biotechnology) Rev 2016 Page 137
House.
3. Bioremediation, 1994 Barker, K.H. and Herson, D.S., Mcgraw Hill, Inc.
New York
4. Waste water Engineering, Metcalf & Eddy , Tata McGraw Hill Publication,
Fourth edition
5. Environmental Science, Richard T. Wright, PHI Pvt. Ltd., Ninth edition
6. EnvironmentalPollutionHealthandToxicology,S.V.S.Rana,NarosaPublishing
House Pvt.Ltd., First edition
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University of Mumbai B. E. (Biotechnology) Rev 2016 Page 138
Course Code Course/Subject Name Credit
BTC802 Bioseperation and Downstream Processing technology -
II 4
Pre-requisites:
Basics of Bioprocesses and Unit Operations.
Basic knowledge of mass balance.
Concepts of molecular di ffusion and di ffusion coe fficients.
Course Objectives:
To cover the fundamentals, and design concepts of various downstream
purification steps (unit operations) involved in a biochemical process.
Course outcomes:
Students will be able to describe theory, principle, design, application and
possible integrations of unit operations in bioprocessing
Module Contents Contact
Hours
01 Adsorption and Ion Exchange:
Introduction to Adsorption, Types of Adsorption, Adsorption
Isotherms, Single Stage Adsorption, Multistage Cross Current
Adsorption, Multistage Counter Current Adsorption,
Equipments for Adsorption, Ion Exchange Equilibria, Ion
Exchange Equipments Design and Construction of
Chromatographic Columns for Bioseparations 10
02 Membrane Separation Techniques:
Membrane sep aration processes: Reverse Osmosis,
Ultrafiltration, Microfiltration, Nanofiltration, Dialysis,
Electrodialysis, Gas Permeation, Pervaporation. Types of
Membranes, Membrane Modules and design. Retention
coefficient, Concentration Polarization, Membrane foulin g
Factors affecting membrane filtration. Advantages of
membrane separation processes over conventional separation
techniques, Industrial Applications 08
03 Equipments for Gas-Liquid Contacting applicable for
Bioprocesses:
Classification of equipments for g as-liquid contacting, Gas
dispersed and liquid continuous phase -Sparged Vessels
(Bubble Columns), Mechanically Agitated Vessels, Tray
Towers, Spray Towers and Spray Chambers, Packed Towers
Comparison of Packed Towers with Tray Towers. 06
04 Crystallization:
Solubility curve, Super saturation, Method of obtaining
supersaturationE ffectofheatonsizeandgrowthofcrystal,RateofC
rystalgrowth and Delta -L law of crystal growth, Material and
energy balance for crystallizers Crystallization equipment -
descr iption 08
04 Drying:
Introduction to drying, Equilibrium, Different types of
moisture contents, Rate of Drying and drying curve, Batch 06
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University of Mumbai B. E. (Biotechnology) Rev 2016 Page 139
Drying and calculation of time of drying, types of driers,
Lyophilisation,
Formulation
Mixing and agitation : Principles of agitation, agitation
equipment, Solid solid mixing equipment, Mixing
effectiveness and Mixing index, Flow patterns in Agitated
vessels, Impellers, Types of impellers, power consumption of
impellers
05 Case Studies of down stream processing:
Baker’s yeast, Ethanol, Citric acid, Penicillin, Insulin,
interferon, Monoclonal antibodies, Tissue plasminogen
activator, Taq polymerase 07
Assessment
Internal
Assessment consists of two tests which should be conducted at proper
intervals.
End Semester theory examination
Question paper will comprise of 6 questions each carrying 20 questions.
Total 4 questions need to be solved
Question no.1 will be compulsory based on entire syllabus wherein sub
questions can be asked.
Remaining questions will be randomly sele cted from all the modules
Weightage of marks should be proportional to number of hours assigned to
each module
References
1. Treybal R.E., Mass transfer operation, 3 Ed., McGraw Hill New York, 1980.
2. McCabe W.L. and Smith J.C., Unit operation in chemical engineering, 5Ed.
McGraw Hill New York 1993.
3. Geankoplis C.J., Transport processed and unit operations, Prentice Hall,
New Delhi 1997.
4. RogerG.Harrison,PaulTodd,ScottR.Rudge,DemetriP.Petrides,Bioseparations
Science and Engineering, Oxford University Press
5. B. Shivshankar, Bioseparations: Priniples and Techniques, Eastern Economy
Edition, PHI Learning Pvt. Ltd., Publishing House, New Delhi, 2012
6. Bioseparation & bioprocessing (2nd Ed.)2 -Volume set, Ed
SUBRAMANIAN Ganapathy, Wiley -VCH, ( 09 -2007)
7. P.A. Belter , E.L. Cussler and Wei -ShouHu., Bioseparations - Downstream
Processing for Biotechnology, Wiley Interscience Publication, 1988.
8. J.E. Bailey and D.F. Ollis, Biochemical Engineering Fundamentals, 2nd
Edition, McGraw Hill, Inc., 1986.
9. R. K. Scopes, Berlin, P rotein Purification: Principles and Practice, Springer,
1982.
10. Scopes Ak, Protein Purification, IRL Press, 1993
11. Biotechnology: Bioprocessing, Rhem and Reed, Vol.3, 1993
12. Separation and purification techniques in biotechnology, Fredreich Dechow,
1989
13. Asenjo J.A. and J.Hong (Eds), Separation Processes in Biotechnolgy, Taylor
and Francis
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University of Mumbai B. E. (Biotechnology) Rev 2016 Page 140
14. T. Schepler et al, Biotreatment, Downstream Processing and Modeling
(Advances in Biochemical Engineering /Biotechnology, Vol 56) by Springer
Verlag
15. Nooralbettu Krishna Prasad, Downstream Process Technology, PHI
Learning, Pvt. Ltd., Eastern Economy Edition.
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University of Mumbai B. E. (Biotechnology) Rev 2016 Page 141
Course Code Course/Subject Name Credits
BTC803 Bioprocess Plant & Equipment Design 4
Pre-requisites:
Process Calculation
Unit operation I and II
Course Objectives:
To impart basic concepts of mechanical and process design of process plant.
To impart design principles for bioreactor.
Course outcomes:
This course makes the students to learn the methods and practices followed
in the design of Bioprocess equipments.
This course makes the students to draw the designed equipments to scale.
Thecourseimpartsadvancedknowledgeonbioreactordesignfore fficientutilizatio
nof the principles in bioprocess technology
Module Contents Contact
Hours
1 Material of construction for process and bioprocess plants.
Mechanical design of process equipment. Design of
cylindrical and spherical vessel under internal and external
pressure. Selection and design of enclosures - flat plate,
formed heads, torispherical and hemispherical heads, standard
flanges and nozzles - classification of flanges, flange thickness
calculation, gasket selection and design, bolt selection and
calculation (Numerical problems
are not needed for design
of flanges, ga sket and nozzles) Design of heat exchange
equipments for upstream and downstream operations in
bioprocessing industries: Heat exchangers process design
(TEMA and IS 4503 standards) of double pipe, single pipe
and multipass shell and tube heat exchangers. 06
2 Introduction to Indian Standards for storage tanks and their
use in design of process vessel. Storage vessels for volatile
andnon -volatile liquids including unfired pressure vessels.
Design of supports - Bracket, leg, saddle and skirt support and
fixed roof and open roof tanks. 06
3 Introduction to general design information for Bioprocess
plants: Development of flowsheet, piping and instrumentation
diagram and its description. General design consideration,
optimum design 06
4 Design of Distillation column:
Detailed design and drawing of perforated plate distillation
column.
Absorption columns: Detailed design and drawing of
perforated plate and packed towers. 06
5 Design of fermenters:
Design considerations for maintaining sterility of process
streams and process equipments. Design of mechanically 06
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University of Mumbai B. E. (Biotechnology) Rev 2016 Page 142
agitated fermenters and non- mechanically agitated (bubble
column and air lift) fermenters.
6 Design of various typ es of evaporators employed in
bioprocess operation:
Evaporators -Standard vertical tube evaporator, single and
multiple effect evaporators and forced circulation evaporator.
Thermal sterilization systems in fermentation processes: batch
and continuous the rmal sterilizers. 06
Term Work
Term work shall consist of minimum eight tutorials from entire syllabus which
are to be given at regular intervals Batch wise.
Tutorials: 20 Marks
Attendance: 05 Marks
Total: 25 Marks
Assessment
Internal
Assessment consists of two tests which should be conducted at proper
intervals.
End Semester theory examination
Question paper will comprise of 6 questions each carrying 20 questions.
Total 4 questions need to be solved
Question no.1 will be compulsory ba sed on entire syllabus wherein sub
questions can be asked.
Remaining questions will be randomly selected from all the modules
Weightage of marks should be proportional to number of hours assigned to
each module
References
1. Sinnott, R.K., Coulson & Richardson’s ’Chemical Engineering’, Volume 6,
3rd Edn., Butterworth Heinemann, New Delhi, 1999.
2. Perry, R.H., etal., Perry’s’ Chemical Engineers Handbook’,7th Edn.,
McGraw Hill, New York, 1997.
3. Joshi , M.V ., and Mahajani, V.V., “Process Equipment Design” , 3rd Edn.,
Macmillan India Limited, New Delhi, 1996.
4. Bownell, L.E., and Young, E.M., ’Process Equipment Design’, Wiley
Eastern, 1968.
5. Peters and Timmerhause, ’Plant Design and Economics for Chemical
Engineers’
6. S.B.Thakore, B.I.Bhatt, “ Introduction to Proce ss Engineering and Design” ,
McGraw Hill Companies
7. Michael L Schuler and Fikret Kargi, ’Bioprocess Engineering’ Printice Hall
of India Pvt. Ltd.
8. Pauline M Doran, ’Bioprocess Engineering Principles’ Academic Press
9. Pressure vessel code -IS Code 2825, B.I.S., New Delhi, 1969
10. Heat Exchanger Design Code IS 4503, B.I.S., New Delhi, 1969
11. Process Equipment Design and Drawing by Kiran Ghadyalji, Nandu
publication
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University of Mumbai B. E. (Biotechnology) Rev 2016 Page 143
Course Code Course/Subject Name Credits
BTE8041 Department Elective IV: Non -conventional Sources of
Energy 4
Pre-requisites:
Knowledge of conventional sources of energy and energy utilization.
Course Objectives:
The main objective of this course is to introduce to the students the current
approaches and technologies in the development of non-conventional
sources of energy their processes to improve environmental quality and
energy requirement, clean and abundant energy, renewable resources and
generate cost e fficient methods to harness energy for human society.
Course outcomes:
Apply their knowledge of energy generation and its conservation to improve
the quality of life in individual context.
Recognize key energy problems and to apply the operating principles and
biotic systems for remediation.
Design, improve and apply biotechnological systems and processes to meet
practical needs of different problems of energy requirement.
Module Contents Contact
Hours
01 Introduction:
Traditional energy systems: fossil fuel, firewood, coal;
Fossil fuel based systems, Impact of fossil fuel based
systems; renewable and non -renewable sources of energy;
global and national energy crisis, Prospects of renewable
energy sources. 03
02 Solar energy:
Solar radiation spectrum, radiation measurements,
applications (heating, cooling, drying, distillation); flat plate
collectors, concentrating collectors, Solar air heaters - types,
solar driers, storage of solar energy -thermal storage, solar
pond, solar water heaters, solar distillation, solar still, solar
cooker, solar heating & cooling of buildings, photovoltaics -
solar cells & its applications 08
03 Wind Energy:
Principle of wind energy conversion; analysis of
aerodynamic forces acting on wind mill blades and
estimation of power output; wind patterns and wind data;
types of wind mills, components of wind mill, site selection. 03
04 Geothermal energy:
Estimation and nature of geothermal energy, geothermal
sources and resources: hydrothermal, geo-pressured hot dry
rock, magma; Advantages, disadvantages and application of
geothermal energy; prospects of geothermal energy in India. 03
05 Energy from the Ocean:
Ocean Thermal Electric Conversion (OTEC ) systems : open
cycle, closed cycle, Hybrid cycle, prospects of OTEC in 05
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University of Mumbai B. E. (Biotechnology) Rev 2016 Page 144
India. Energy from tides, basic principle of tidal power,
single basin and double basin tidal power plants,
advantages, limitation and scope of tidal energy. Wave
energy and power from wave, wave energy conversion
devices, advantages and disadvantages of wave energy.
06 Energy from Biomass:
Biomass conversion principle: combustion and
fermentation; Biogas generation plants: classification,
advantages and disadvantages, constructional details, site
selection ,digester design consideration, filling a digester for
starting, maintaining biogas production, Fuel properties of
bio gas, utilization of biogas.
Biodiesel : principle, production, efficiency , scope in India. 05
07 Fuel cells :
Introduction, Design principle, operation of fuel cell, Types
of fuel cells, conversion efficiency of fuel cell, and
application of fuel cells.
Microbial Fuel cells: Principle, construction, working,
efficiency and scope in India. 04
08 Hydrogen energy:
Introduction, Hydrogen Production methods, Hydrogen
storage, hydrogen transportation, utilization of hydrogen
gas, hydrogen as alternative fuel for vehicles. Nuclear
energy: nuclear reactors, fission and fusion reactions;
advantages and disadvantages of nuclear energy. 04
Term Work
Term work shall consist of minimum eight tutorials from entire syllabus which
are to be given at regular intervals Batch wise.
Tutorials: 20 Marks
Attendance: 05 Marks
Total: 25 Marks
Assessment
Internal
Assessment consists of two tests which should be conducted at proper
intervals.
End Semester theory examination
Question paper will comprise of 6 questions each carrying 20 questions.
Total 4 questions need to be solved
Question no.1 will be compulsory based on entire syllabus wherein sub
questions can be asked.
Remaining questions will be randomly selected from all the modules
Weightage of marks should be proportional to number of hours assigned to
each module
References
1. Non-conventional energy sources by G.D. Rai, Khanna Publishers
2. Solar Energy: Fundamentals and Applications by H.P. Garg & Jai Prakash,
Tata McGraw Hill
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University of Mumbai B. E. (Biotechnology) Rev 2016 Page 145
3. Solar Engineering of Thermal Processes by Duc and Beckman, John Wiley
4. Solar Energy: Principles of Thermal Collection and Storage by S,P
Sukhatme, Tata McGraw Hill
5. Non-Conventional Energy Resources, B H Khan, Tata McGraw -Hill
Education, Jan -2006
6. Non-Conventional Energy Resources by S.Hasan Saeed and D.K.Sharma
7. Fuel Cells by Bockris and Srinivasan; McGraw Hill
Page 146
University of Mumbai B. E. (Biotechnology) Rev 2016 Page 146
Course Code Course/Subject Name Credits
BTE8042 Department Elective IV: Total Quality Management 4
Prerequisites:
Knowledge of Basic Engi neering and Science.
Course Objectives:
To acquaint with the significance and features of TQM philosophy.
To familiarize with various quality tools and their uses in problem solving.
To appraise on the modern productivity improvement approaches and their
interface with TQM
To familiarize with various quality standards, quality auditing and
certification methodology.
To give and an insight into t he ongoing global trends in quality approach and
practices with special forms to the customer relationship
Course Outcomes:
Learner will be able to:
Appreciate the importance of quality and its dimensions in striving for
excellence
Understand the conscious compromise between cost and quality
Develop competency in the selection in various manufacturing and service
functions
Develop competency in the use of appropriate quality tools in various
manufacturing and service functions
Integrate quality approaches f or productivity improvement.
Acquire knowledge base and develop skills for conducting quality audits
Module Contents Contact
Hours
1 Introduction:
Definition of Quality, principles and dimensions of TQM
Quality in manufacturing and service segments. Approach in
implementation of TQM, barriers in implementation. Cost of
quality prevention, appraisal and failure costs, hidden costs,
trade -o between quality and cost. 8
2 Planning for quality and Quality improvement:
Planning for quality: Need for qualit y policies and objective.
Significance of top management commitment, strategic
planning for quality. Quality improvement: Management of
controllable defects, operator controllable defects, sporadic
and chronic problems of operator controllable defects,
sporadic and chronic problems of quality, Pareto's principle.
Bench marking: Definition and significance, data collection
for bench marking and its use. 8
3 Customer relations:
Customers, user and consumers, product awareness, types of
customers, customer pe rception and expectations. Quality
feedback and redressal. Basic principles of reliability: quality 8
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University of Mumbai B. E. (Biotechnology) Rev 2016 Page 147
and reliability, Product life cycle, trade -o between
maintainability.
4 Vendor relations:
Vendor as a partner, vendor selection, vendor evaluation.
Push Pull view of supply chain and cycle view of chain
management 8
5 SQC Tool:
Histograms, Pie charts, Scatter diagrams, Cause and diagram
etc.
Statistical Process Control:
Process variability: Variables and process variation, measures
of accuracy and centering, precision or spread, normal
distribution
Process Control: Control charts for variables (X -chart, R -
chart , -chart) and attributes (np -charts, p -chart, c -charts, U -
chart ) Process capability: OC curve, acceptance sampling,
single and double sampl ing producer's and consumer's risk. 8
6 Quality System:
Quality standards:
ISO 9001:2000 Quality management system. ˆ
ISO 14001:2004 Environmental management system.
ISO 27001:2005 Information security management system.
Quality assurance: Nature of assu rance, reports on quality,
measuring performance, internal audit, surveillance audit,
quality certification methodology and implications
Productivity improvement Tools/ Approaches/ Techniques:
Principles of Six -Sigma, approaches like JIT, Lean
manufacturin g zero defect concept, KANBAN, QFD, FMEA,
Basics of DOE and Shining concepts of quality. Productivity
improvement techniques like 5S, POKAYOKE, SMED,
KAIZEN and Concurrent Engineering. 8
Term Work
Term work shall consist of minimum eight tutorials from entire syllabus which
are to be given at regular intervals Batch wise.
Tutorials: 20 Marks
Attendance: 05 Marks
Total: 25 Marks
Assessment
Internal
Assessment consists of two tests which should be conducted at proper
intervals.
End Semester theory examination
Question paper will comprise of 6 questions each carrying 20 questions.
Total 4 questions need to be solved
Question no.1 will be compulsory based on entire syllabus wherein sub
questions can be asked.
Remaining questions will be random ly selected from all the modules
Weightage of marks should be proportional to number of hours assigned to
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University of Mumbai B. E. (Biotechnology) Rev 2016 Page 148
each module
References
1. Juran, J. M., Gryana, F. M., Quality planning and analysis, TMH.
2. Bester Fidd, D. H., et.al. Total quality management, Prentice Hall.
3. Erossbly, Pillip b., Quality is free, Mentor/New Americal Library.
4. Ishikawa, K., What is total quality control? The Japanese way, Prentice Hall
5. Fergenbaum, Armand V., Total quality control.
6. Logothetis, N., Managing for total quality, Prentice Hall
7. Aurora, K. C., Total Quality Management, S. K. Kataria and Sons
8. Haldar, U. K., Total Quality Management, Dhanpatrai and Co.
Page 149
University of Mumbai B. E. (Biotechnology) Rev 2016 Page 149
Course Code Course/Subject Name Credits
BTE8043 Department Elective IV: Entrepreneurship 4
Pre-requisites:
Basic Knowledge of Engineering Science, Mathematics and Interest towards
finance
Course Objectives:
Meaning and concept of entrepreneurship
Preparing a Business Plan
Financing the New Venture
Managing Growth in New Venture
skills required to be an entrepreneur
Course outcomes:
Students will be able to make decision in new venture
Students will have creativity in Entrepreneurship.
Students will develop skill for innovation and competition.
Students learn leadership qualities.
Module Contents Contact
Hours
1 Introduction to Entrepreneurship: Meaning and concept of
entrepreneurship, the history of entrepreneurship
development, role of entrepreneurship in economic
development, agencies in entrepreneurship management and
future of entrepreneurship. 04
2 The Entrepreneur: Meaning of entrepreneur, the skills
required to be an entrepreneur, the entrepreneurial decision
process, and role models, mentors and support system. 04
3 Business Opportunity Identification: Business ideas, m ethods
of generating ideas, and opportunity recognition 05
4 Preparing a Business Plan: Meaning and significance of a
business plan, components of a business plan, and feasibility
study 05
5 Financing the New Venture: Importance of new venture
financing, types of ownership securities, venture capital, types
of debt securities, determining ideal debt -equity mix, and
financial institutions and banks 05
6 Launching the New Venture: Choosing the legal form of new
venture, protection of intellectual property, and marketing the
new venture
04
7 Managing Growth in New Venture: Characteristics of high
growth new ventures, strategies for growth, and building the
new venture capital 04
8 Harvesting Rewards: Exit strategies for entrepreneurs,
bankruptcy, and succession and harvesting strategy 04
Term Work
Term work shall consist of minimum eight tutorials from entire syllabus which
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University of Mumbai B. E. (Biotechnology) Rev 2016 Page 150
are to be given at regular intervals Batch wise.
Tutorials: 20 Marks
Attendance: 05 Marks
Total: 25 Marks
Assessment
Internal
Assessment consists of two tests which should be conducted at proper
intervals.
End Semester theory examination
Question paper will comprise of 6 questions each carrying 20 questions.
Total 4 questions need to be solved
Question no.1 will be compulsory based on entire syllabus wherein sub
questions can be asked.
Remaining questions will be randomly selected from all the modules
Weightage of marks should be proportional to number of hours assigned to
each module
References
1. Entrepreneurship, Hisrich Peters Sphephard, Tata McGraw Hill
2. Fundamentals of entrepreneurship, S.K. Mohanty, Published by PHI
Learning, 2010
3. Management and Entrepreneurship, By N. V. R. Naidu, T. Krishna Rao
4. Entrepreneurship in Theory and Practice: Paradox es in Play by S. L. Nielsen,
K. Klyver, M. Rostgaard Evald Published by Edward Elgar Publishing Ltd;
2012
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University of Mumbai B. E. (Biotechnology) Rev 2016 Page 151
Course Code Course/Subject Name Credits
BTE8044 Department Elective IV: Advanced Bioinformatics 4
Pre-requisites:
Bioinformatics, Knowledge of protein structure.
Course Objectives:
Study the development and implementation of tools that enables to e fficiently
access and manage various types of information.
Study the development of new algorithms (mathematical formulas ) and
statistics used to assess relationships among members of large data sets. For
example, methods to locate a gene within a sequence, predict protein
structure and/or function, and cluster protein sequences into families of
related sequences.
The primary goal of bioinformatics is to increase the understanding of
biological processes. What sets it apart from other approaches, however, is
its focus on developing and applying computationally intensive techniques to
achieve this goal.
Help have a b etter knowledge of pharmaceutical biology & its relation with
information technology.
Course outcomes:
By learning this course the students will be able to:
Describe the contents and properties of the most important bio informatical
databases, perform te xt-and sequence -based searches, and analyse and
discuss the results in light of molecular biological knowledge
Explain the major steps in pair wise and multiple sequence alignment,
explain the principle for, and execute pair wise sequence alignment by
dynamic programming
Explain the major features of evolution of genes and proteins and explain
how different methods can be used to construct phylogenetic trees.
Explainthemajorfeaturesofmethodsformodellingproteinstructuresanduseprogr
amsfor visualizing and analysing such structures.
Give examples of methods for describing and analysing genes, genomes and
gene expressi on
To solve any biological sequence analysis problem, with choosing &
modifying suitable computational model to solve it.
Module Contents Contact
Hours
01 Introduction to concepts of molecular modelling Methods
of molecular modelling: Molecular mechanics, Abinitio
Quantum mechanics, Semi empirical quantum mechanics.
Energy minimization of molecules: local & global energy
minima. 10
02 Overview: Machine learning, Genetic algorithms,
Simulated annealing
Interoperability: Introduction, Its role i n bioinformatics.
Interexchange Languages: XML, CORBA and UMLS. 08
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University of Mumbai B. E. (Biotechnology) Rev 2016 Page 152
Clustering algorithms.
03 Drug discovery, Markov chains, Hidden markov models. 06
04 Drug designing:
Drug optimization, Identification of pharmacophore,
Optimizing access to target, Prodru gs, Endogenous
compounds as drugs, Quantitative structure - activity
relationship (QSAR). 05
05 Docking: Introduction, Protein protein docking, Protein
Ligand docking, Applications of docking. 06
Term Work
Term work shall consist of minimum eight tutorials from entire syllabus which
are to be given at regular intervals Batch wise.
Tutorials: 20 Marks
Attendance: 05 Marks
Total: 25 Marks
Assessment
Internal
Assessment consists of two tests which should be conducted at proper
intervals.
End Semester theory examination
Question paper will comprise of 6 questions each carrying 20 questions.
Total 4 questions need to be solved
Question no.1 will be compulsory based on entire syllabus wherein sub
questions can be asked.
Remaining questions will be randomly selected from all the modules
Weightage of marks should be proportional to number of hours assigned to
each module
References
1. Cynthia Gibas & Per Jambeck, Developing Bioinformatics computer skills,
third edition.
2. S.C. Rastogi, Bioinformatics concepts, skills & applications, first edition.
3. Dov Stekel, Microarray Bioinformatics.
4. David W. Mount, Bioinformatics sequence and genome analysis.
5. N.Claude Cohen, Molecular modeling in drug design.
6. Oreilly, Developing bioin formatics computer skills, Shro ff publishers, 1st
Indian edition.
7. Medicinal Chemistry by Graham L. Patrick, Oxford University Press
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University of Mumbai B. E. (Biotechnology) Rev 2016 Page 153
Course Code Course Name Credits
ILO8021 Institute Level Optional Subject II - Project
Management 03
Objectives:
To familiarize the students with the use of a structured methodology/approach
for each and every unique project undertaken, including utilizing project
management concepts, tools and techniques.
To appraise the students with the project managem ent life cycle and make
them knowledgeable about the various phases from project initiation through
closure.
Outcomes:
Learner will be able to…
Apply selection criteria and select an appropriate project from different
options.
Write work break down structure for a project and develop a schedule based
on it.
Identify opportunities and threats to the project and decide an approach to deal
with them strategically.
Use Earned value technique and determine & predict status of the project.
Capture lessons learned during project phases and document them for future
reference
Module Detailed Contents Contact
Hours
1 Project Management Foundation:
Definition of a project, Project Vs Operations, Necessity of
project management, Triple constraints, Project life cycles
(typical & atypical) Project phases and stage gate process.
Role of project manager. Negotiations and resolving
conflicts. Project management in various organization
structures. PM knowledge areas as per Project Management
Institute (PMI). 5
2 Initiating Projects:
How to get a project started, Selecting project strategically,
Project selection models (Numeric /Scoring Models and Non -
numeric models), Project portfolio process, Project sponsor
and creating charter; Project proposal. Effective pro ject
team, Stages of team development & growth (forming,
storming, norming & performing), team dynamics. 6
3 Project Planning and Scheduling:
Work Breakdown structure (WBS) and linear responsibility
chart, Interface
Co-ordination and concurrent engineer ing, Project cost
estimation and budgeting, Top down and bottoms up
budgeting, Networking and Scheduling techniques. PERT,
CPM, GANTT chart. Introduction to Project Management
Information System (PMIS). 8
4 Planning Projects: 6
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University of Mumbai B. E. (Biotechnology) Rev 2016 Page 154
Crashing project time, Resou rce loading and leveling,
Goldratt's critical chain, Project Stakeholders and
Communication plan.
Risk Management in projects: Risk management planning,
Risk identification and risk register. Qualitative and
quantitative risk assessment, Probability and im pact matrix.
Risk response strategies for positive and negative risks
5 Executing Projects:
Planning monitoring and controlling cycle. Information needs
and reporting, engaging with all stakeholders of the projects.
Team management, communication and project meetings.
5.2 Monitoring and Controlling Projects:
Earned Value Management techniques for measuring value of
work completed; Using milestones for measurement; change
requests and scope creep. Project audit.
5.3 Project Contracting
Project procurem ent management, contracting and
outsourcing, 8
6 Project Leadership and Ethics:
Introduction to project leadership, ethics in projects.
Multicultural and virtual projects.
6.2 Closing the Project:
Customer acceptance; Reasons of project termination,
Various types of project terminations (Extinction, Addition,
Integration, Starvation), Process of project termination,
completing a final report; doing a lessons learned analysis;
acknowledging successes and failures; Project management
templates and ot her resources; Managing without authority;
Areas of further study. 6
Assessment
Internal
Assessment consists of two tests which should be conducted at proper
intervals.
End Semester theory examination
Question paper will comprise of 6 questions each carrying 20 questions.
Total 4 questions need to be solved
Question no.1 will be compulsory based on entire syllabus wherein sub
questions can be asked.
Remaining questions will be randomly selected from all the modules
Weightage of marks should be propor tional to number of hours assigned to
each module
References
1. Jack Meredith & Samuel Mantel, Project Management: A managerial
approach, Wiley India, 7thEd.
2. A Guide to the Project Management Body of Knowledge (PMBOK® Guide),
5th Ed, Project Management Instit ute PA, USA
3. Gido Clements, Project Management, Cengage Learning.
4. Gopalan, Project Management, , Wiley India
5. Dennis Lock, Project Management, Gower Publishing England, 9 th Ed.
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University of Mumbai B. E. (Biotechnology) Rev 2016 Page 155
Course Code Course Name Credits
ILO8022 Institute Level Optional Subject II - Finance
Management 03
Objectives:
Overview of Indian financial system, instruments and market
Basic concepts of value of money, returns and risks, corporate finance,
working capital and its management
Knowledge about sources of finance, capital structure, dividend policy
Outcomes:
Learner will be able to…
Understand Indian finance system and corporate finance
Take investment, finance as well as dividend decisions
Module Detailed Contents Contact
Hours
01 Overview of Indian Financial System: Characteristics,
Components and Functions of Financial System.
Financial Instruments: Meaning, Characteristics and
Classification of Basic Financial Instruments — Equity Shares,
Preference Shares, Bonds -Debentures, Certificates of Deposit,
and Treasury Bil ls.
Financial Markets: Meaning, Characteristics and Classification
of Financial Markets — Capital Market, Money Market and
Foreign Currency Market
Financial Institutions: Meaning, Characteristics and
Classification of Financial Institutions — Commercial Ba nks,
Investment -Merchant Banks and Stock Exchanges 06
02 Concepts of Returns and Risks: Measurement of Historical
Returns and Expected Returns of a Single Security and a Two -
security Portfolio; Measurement of Historical Risk and
Expected Risk of a Single Security and a Two -security
Portfolio.
Time Value of Money: Future Value of a Lump Sum, Ordinary
Annuity, and Annuity Due; Present Value of a Lump Sum,
Ordinary Annuity, and Annuity Due; Continuous
Compounding and Continuous Discounting. 06
03 Overview of Corporate Finance: Objectives of Corporate
Finance; Functions of Corporate Finance —Investment
Decision, Financing Decision, and Dividend Decision.
Financial Ratio Analysis: Overview of Financial Statements —
Balance Sheet, Profit and Loss Account, and Cash Flow
Statement; Purpose of Financial Ratio Analysis; Liquidity
Ratios; Efficiency or Activity Ratios; Profitability Ratios;
Capital Structure Ratios; Stock Market Ratios; Limitations of
Ratio Analysis. 09
04 Capital Budgeting: Meaning and Importance of Ca pital
Budgeting; Inputs for Capital Budgeting Decisions; Investment
Appraisal Criterion —Accounting Rate of Return, Payback 10
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University of Mumbai B. E. (Biotechnology) Rev 2016 Page 156
Period, Discounted Payback Period, Net Present Value(NPV),
Profitability Index, Internal Rate of Return (IRR), and
Modified Internal Rate of Return (MIRR)
Working Capital Management: Concepts of Meaning Working
Capital; Importance of Working Capital Management; Factors
Affecting an Entity’s Working Capital Needs; Estimation of
Working Capital Requirements; Management of Inventories;
Management of Receivables; and Management of Cash and
Marketable Securities.
05 Sources of Finance: Long Term Sources —Equity, Debt, and
Hybrids; Mezzanine Finance; Sources of Short Term
Finance —Trade Credit, Bank Finance, Commercial Paper;
Project Finance.
Capital Structure: Factors Affecting an Entity’s Capital
Structure; Overview of Capital Structure Theories and
Approaches — Net Income Approach, Net Operating Income
Approach; Traditional Approach, and Modigliani -Miller
Approach. Relation between C apital Structure and Corporate
Value; Concept of Optimal Capital Structure 05
06 Dividend Policy: Meaning and Importance of Dividend Policy;
Factors Affecting an Entity’s Dividend Decision; Overview of
Dividend Policy Theories and Approaches —Gordon’s
Appr oach, Walter’s Approach, and Modigliani -Miller
Approach 03
Assessment
Internal
Assessment consists of two tests which should be conducted at proper
intervals.
End Semester theory examination
Question paper will comprise of 6 questions each carrying 20 questions.
Total 4 questions need to be solved
Question no.1 will be compulsory based on entire syllabus wherein sub
questions can be asked.
Remaining questions will be randomly selected from all the modules
Weightage of marks should be proportional to nu mber of hours assigned to
each module
References
1. Fundamentals of Financial Management, 13th Edition (2015) by Eugene F.
Brigham and Joel F. Houston; Publisher: Cengage Publications, New Delhi.
2. Analysis for Financial Management, 10th Edition (2013) by Rober t C.
Higgins; Publishers: McGraw Hill Education, New Delhi.
3. Indian Financial System, 9th Edition (2015) by M. Y. Khan; Publisher:
McGraw Hill Education, New Delhi.
4. Financial Management, 11th Edition (2015) by I. M. Pandey; Publisher: S.
Chand (G/L) & Compa ny Limited, New Delhi.
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University of Mumbai B. E. (Biotechnology) Rev 2016 Page 157
Course Code Course Name Credits
ILO8023 Institute Level Optional Subject II - Entrepreneurship
Development and Management 03
Objectives:
To acquaint with entrepreneurship and management of business
Understand Indian environment for entrepreneurship
Idea of EDP, MSME
Outcomes:
Learner will be able to…
Understand the concept of business plan and ownerships
Interpret key regulations and legal aspects of entrepreneurship in India
Understand government policies for entrepreneurs
Module Detailed Contents Contact
Hours
01 Overview Of Entrepreneurship: Definitions, Roles and
Functions/Values of Entrepreneurship, History of
Entrepreneurship Development, Role of Entrepreneurship in
the National Economy, Functions of an Entrepreneur,
Entrepreneurship and Forms of Business Ownership
Role of Money and Capital Markets in Entrepreneurial
Development: Contribution of Government Agencies in
Sourcing information for Entrepreneurship 04
02 Business Plans And Importance Of Capital To
Entrepreneurship: Preliminary and Marketing Plans,
Management and Personnel, Start -up Costs and Financing as
well as Projected Financial Statements, Legal Section,
Insurance, Suppliers and Risks, Assumptions and Conclusion,
Capital and its Importance to the Entrepreneur
Entrepreneurship And Business Development: Starting a New
Business, Buying an Existing Business, New Product
Development, Business Growth and the Entrepreneur Law and
its Relevance to Business Ope rations 09
03 Women’s Entrepreneurship Development, Social
entrepreneurship -role and need, EDP cell, role of sustainability
and sustainable development for SMEs, case studies, exercises 05
04 Indian Environment for Entrepreneurship: key regulations and
legal aspects , MSMED Act 2006 and its implications, schemes
and policies of the Ministry of MSME, role and responsibilities
of various government organisations, departments, banks etc.,
Role of State governments in terms of infrastructure
developments and support etc., Public private partnerships,
National Skill development Mission, Credit Guarantee Fund,
PMEGP, discussions, group exercises etc 08
05 Effective Management of Business: Issues and problems faced
by micro and small enterprises and effective ma nagement of M
and S enterprises (risk management, credit availability,
technology innovation, supply chain management, linkage with 08
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University of Mumbai B. E. (Biotechnology) Rev 2016 Page 158
large industries), exercises, e -Marketing
06 Achieving Success In The Small Business: Stages of the small
business life cycle, four types of firm -level growth strategies,
Options – harvesting or closing small business Critical Success
factors of small business 05
Assessment
Internal
Assessment consists of two tests which should be conducted at proper
intervals.
End Semester theory examination
Question paper will comprise of 6 questions each carrying 20 questions.
Total 4 questions need to be solved
Question no.1 will be compulsory based on entire syllabus wherein sub
questions can be asked.
Remaining questions will be randomly selected from all the modules
Weightage of marks should be proportional to number of hours assigned to
each module
References
1. Poornima Charantimath, Entrepreneurship development - Small Business
Enterprise, Pearson
2. Education Robert D Hisrich, Mi chael P Peters, Dean A Shapherd,
Entrepreneurship, latest edition, The McGraw Hill Company
3. Dr TN Chhabra, Entrepreneurship Development, Sun India Publications, New
Delhi
4. Dr CN Prasad, Small and Medium Enterprises in Global Perspective, New
century Publicat ions, New Delhi
5. Vasant Desai, Entrepreneurial development and management, Himalaya
Publishing House
6. Maddhurima Lall, Shikah Sahai, Entrepreneurship, Excel Books
7. Rashmi Bansal, STAY hungry STAY foolish, CIIE, IIM Ahmedabad
8. Law and Practice relating to M icro, Small and Medium enterprises, Taxmann
Publication Ltd.
9. Kurakto, Entrepreneurship - Principles and Practices, Thomson Publication
10. Laghu Udyog Samachar
11. www.msme.gov.in
12. www.dcmesme.gov.in
13. www.msmetraining.gov.in
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University of Mumbai B. E. (Biotechnology) Rev 2016 Page 159
Course Code Course Name Credits
ILO8024 Institute Level Optional Subject II - Human Resource
Management 03
Objectives:
To introduce the students with basic concepts, techniques and practices of the
human resource management.
To provide opportunity of learning Human resource management (HRM)
processes, related with the functions, and challenges in the emerging
perspective of today’s organizations.
To familiarize the students about the latest developments, trends & different
aspects of HRM.
To acquaint the student with the impo rtance of inter -personal & inter -group
behavioural skills in an organizational setting required for future stable
engineers, leaders and managers.
Outcomes:
Learner will be able to…
Understand the concepts, aspects, techniques and practices of the human
resource management.
Understand the Human resource management (HRM) processes, functions,
changes and challenges in today’s emerging organizational perspective.
Gain knowledge about the latest developments and trends in HRM.
Apply the knowledge of behavioura l skills learnt and integrate it with in inter
personal and intergroup environment emerging as future stable engineers and
managers.
Module Detailed Contents Contact
Hours
01 Introduction to HR
Human Resource Management - Concept, Scope and
Importance, Interdisciplinary Approach Relationship with
other Sciences, Competencies of HR Manager, HRM
functions.
Human resource development (HRD): changing role of HRM
– Human resource Planning, Technological change,
Restructuring and rightsizing, Empowerment, TQM,
Managing ethical issues. 5
02 Organizational Behaviour (OB)
Introduction to OB Origin, Nature and Scope of
Organizational Behaviour , Relevance to Organizational
Effectiveness and Contemporary issues
Personality: Meaning and Determinants of Personality,
Personality development, Personality Types, Assessment of
Personality Traits for Increasing Self Awareness
Perception: Attitude and Value, Effect of perception on
Individual Decision -making, Attitude and Behavior.
Motivation: Theories of Motivation and their Applications for
Behavioral Change (Maslow, Herzberg, McGregor);
Group Behaviour and Group Dynamics: Work groups formal 7
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University of Mumbai B. E. (Biotechnology) Rev 2016 Page 160
and informal groups and stages of group development. Team
Effectiveness: High performing teams, Team Roles, cross
functional and self-directed team. Case study
03 Organizational Structure & Design
Structure, size, technology, Environment of organization;
Organizational Roles & conflicts: Concept of roles; role
dynamics; role conflicts and stress.
Leadership: Concepts and skills of leadership, Leadership and
managerial roles, Leadership styles and contemporary issues
in leadership.
Power and Politics: Sources and uses of power; Politics at
workplace, Tactics and strategies. 6
04 Human resource Planning
Recruitment and Selection process, Job -enrichment,
Empowerment - Job-Satisfaction, employee morale.
Performance Appraisal Systems: Traditional & modern
methods, Performance Counselling , Career Planning.
Training & Development: Identification of Training Needs,
Training Methods 5
05 Emerging Trends in HR
Organizational development; Business Process Re -engineering
(BPR), BPR as a tool for organizational development ,
managing processes & transformation in HR. Organizational
Change, Culture, Environment
Cross Cultural Leadership and Decision Making: Cross
Cultural Communication and diversity at work, Causes of
diversity, managing diversity with special reference to
handicapped, women and ageing people, intra company
cultural difference in employee motivation. 6
06 HR & MIS
Need, purpose, objective and role of information system in
HR, Applications in HRD in various industries (e.g.
manufacturing R&D, Public Transport, Hospitals, Hotels and
service industries
Strategic HRM
Role of Strategic HRM in the modern business world, Concept
of Strategy, Strategic Management Process, Approaches to
Strategic Decision Making; Strategic Intent – Corporate
Mission, Vision, Objectives and Goals
Labor Laws & Industrial Relations
Evolution of IR, IR issues in organizations, Overview of
Labor Laws in India; Industrial Disputes Act, Trade Unions
Act, Shops and Establishments Act 10
Assessment
Internal
Assessment consists of two tests which should be conducted at proper
intervals.
End Semester theory examination
Question paper will comprise of 6 questions each carrying 20 questions.
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University of Mumbai B. E. (Biotechnology) Rev 2016 Page 161
Total 4 questions need to be solved
Question no.1 will be compulsory based on entire syllabus wherein sub
questions can be asked.
Remaining questions will be randomly selected from all the modules
Weightage of marks s hould be proportional to number of hours assigned to
each module
References
1. Stephen Robbins, Organizational Behaviour , 16th Ed, 2013
2. V S P Rao, Human Resource Management, 3rd Ed, 2010, Excel publishing
3. Aswathapa, Human resource management: Text & cases, 6t h edition, 2011
4. C. B. Mamoria and S V Gankar, Dynamics of Industrial Relations in India,
15th Ed, 2015, Himalaya Publishing, 15thedition, 2015
5. P. Subba Rao, Essentials of Human Resource management and Industrial
relations, 5th Ed, 2013, Himalaya Publishin g
6. Laurie Mullins, Management & Organizational Behaviour , Latest Ed, 2016,
Pearson Publications
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University of Mumbai B. E. (Biotechnology) Rev 2016 Page 162
Course Code Course Name Credits
ILO8025 Institute Level Optional Subject II - Professional
Ethics and Corporate Social Responsibility (CSR) 03
Objectives:
To understand professional ethics in business
To recognized corporate social responsibility
Outcomes:
Learner will be able to…
Understand rights and duties of business
Distinguish different aspects of corporate social responsibility
Demonstrate professional ethics
Understand legal aspects of corporate social responsibility
Module Detailed Contents Contac
t
Hours
01 Professional Ethics and Business: The Nature of Business
Ethics; Ethical Issues in Business; Moral Responsibility and
Blame; Utilitarianism: Weighing Social Costs and Benefits;
Rights and Duties of Business 04
02 Professional Ethics in the Marketplace: Perfect Competition;
Monopoly Competition; Oligopolistic Competition; Oligopolies
and Public Policy
Professional Ethics and the Environment: Dimensions of
Pollution and Resource Depletion; Ethics of Pollution Control;
Ethics of Conserving Depletable Resources 08
03 Professional Ethics of Consumer Protection: Markets and
Consumer Protection; Contract View of Business Firm’s Duties
to Consumers; Due Care Theory; Advertising Ethics; Consumer
Privacy
Professional Ethics of Job Discrimination: Nature of Job
Discrimination; Extent of Discrimination; Reservation of Jobs. 06
04 Introduction to Corporate Social Responsibility: Potential
Business Benefits —Triple bottom line, Human resources, Risk
management, Supplier relations; Criticisms and concerns —
Nature of business; Motives; Misdirection.
Trajectory of Corporate Social Responsibility in India 05
05 Corporate Social Responsibility: Articulation of Gandhian
Trusteeship
Corporate Social Responsibility and Small and Medium
Enterprises (SMEs) in India, Corporate Social Responsibility
and Public -Private Partnership (PPP) in India 08
06 Corporate Social Responsibility in Globalizing India : Corporate
Social Responsibility Voluntary Guidelines, 2009 issued by the
Ministry of Corporate Affairs, Government of India, Legal
Aspects of Corporate Social Responsibility —Companies Act,
2013. 08
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University of Mumbai B. E. (Biotechnology) Rev 2016 Page 163
Assessment
Internal
Assessment consists of two tests which should be conducted at proper
intervals.
End Semester theory examination
Question paper will comprise of 6 questions each carrying 20 questions.
Total 4 questions need to be solved
Question no.1 will be compulsory based on entire syllabus wherein su b
questions can be asked.
Remaining questions will be randomly selected from all the modules
Weightage of marks should be proportional to number of hours assigned to
each module
References
1. Business Ethics: Texts and Cases from the Indian Perspective (2013) by
Ananda Das Gupta; Publisher: Springer.
2. Corporate Social Responsibility: Readings and Cases in a Global Context
(2007) by Andrew Crane, Dirk Matten, Laura Spence; Publisher: Routledge.
3. Business Ethics: Concepts and Cases, 7th Edition (2011) by Manuel G.
Velasquez; Publisher: Pearson, New Delhi.
4. Corporate Social Responsibility in India (2015) by Bidyut Chakrabarty,
Routledge, New Delhi.
Page 164
University of Mumbai B. E. (Biotechnology) Rev 2016 Page 164
Course Code Course Name Credits
ILO8026 Institute Level Optional Subject II - Research
Methodology 03
Objectives:
To understand Research and Research Process
To acquaint students with identifying problems for research and develop
research strategies
To familiarize students with the techniques of data collection, analysis of data
and interpretation
Outcomes :
Learner will be able to…
Prepare a preliminary research design for projects in their subject matter
areas
Accurately collect, analyze and report data
Present complex data or situations clearly
Review and analyze research findings
Module Detailed Contents Contact
Hours
01 Introduction and Basic Research Concepts
1.1 Research – Definition; Concept of Construct, Postulate,
Proposition, Thesis, Hypothesis, Law, Principle. Research
methods vs Methodology
1.2 Need of Research in Business and Social Sci ences
1.3 Objectives of Research
1.4 Issues and Problems in Research
1.5 Characteristics of Research: Systematic, Valid, Verifiable,
Empirical and Critical 09
02 Types of Research
2.1. Basic Research
2.2. Applied Research
2.3. Descriptive Research
2.4. Analytical Research
2.5. Empirical Research
2.6 Qualitative and Quantitative Approaches 07
03 Research Design and Sample Design
3.1 Research Design – Meaning, Types and Significance
3.2 Sample Design – Meaning and Significance Essentials of a
good sampling Stages in Sample Design Sampling
methods/techniques Sampling Errors 07
04 Research Methodology
4.1 Meaning of Research Methodology
4.2. Stages in Scientific Research Process:
a. Identification and Selection of Research Problem
b. Formulation of Research Problem
c. Review of Literature
d. Formulation of Hypothesis
e. Formulation of research Design 08
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University of Mumbai B. E. (Biotechnology) Rev 2016 Page 165
f. Sample Design
g. Data Collection
h. Data Analysis
i. Hypothesis testing and Interpretation of Data
j. Preparation of Research Report
05 Formulating Research Problem
5.1 Considerations: Relevance, Interest, Data Availability,
Choice of data, Analysis of data, Generalization and
Interpretation of analysis 04
06 Outcome of Research
6.1 Preparation of the report on conclusion reached
6.2 Validity Testing & Ethical Issues
6.3 Suggestions and Recommendation 04
Assessment
Internal
Assessment consists of two tests which should be conducted at proper
intervals.
End Semester theory examination
Question paper will comprise of 6 questions each carrying 20 questions.
Total 4 questions need to be solved
Question no.1 will be compulsory based on entire syllabus wherein sub
questions can be asked.
Remaining questions will be randomly selected from all the modules
Weightage of marks should be p roportional to number of hours assigned to
each module
References
1. Dawson, Catherine, 2002, Practical Research Methods, New Delhi, UBS
Publishers Distributors.
2. Kothari, C.R., 1985, Research Methodology -Methods and Techniques, New
Delhi, Wiley Eastern Limite d.
3. Kumar, Ranjit, 2005, Research Methodology -A Step -by-Step Guide for
Beginners, (2nded), Singapore, Pearson Education
Page 166
University of Mumbai B. E. (Biotechnology) Rev 2016 Page 166
Course Code Course Name Credits
ILO8027 Institute Level Optional Subject II - IPR and
Patenting 03
Objectives:
To understand intellectual property rights protection system
To promote the knowledge of Intellectual Property Laws of India as well as
International treaty procedures
To get acquaintance with Patent search and patent filing procedure and
applications
Outcomes:
understand Intellectual Property assets
assist individuals and organizations in capacity building
work for development, promotion, protection, compliance, and enforcement of
Intellectual Property and Patenting
Module Detailed Contents Contact
Hours
01 Introduction to Intellectual Property Rights (IPR): Meaning of
IPR, Different category of IPR instruments - Patents,
Trademarks, Copyrights, Industrial Designs, Plant variety
protection, Geographical indications, Transfer of technology etc.
Importance of IPR in Modern Global Economic Environment:
Theories of IPR, Philosophical aspects of IPR laws, Need for
IPR, IPR as an instrument of development 05
02 Enforcement of Intellectual Property Rights: Introduction,
Magnitude of problem, Factors that create and sustain
counterfeiting/piracy, International agreements, International
organizations (e.g. WIPO, WTO) active in IPR enforcement
Indian Scenario of IPR : Introduction, History of IPR in India,
Overview of IP laws in India, Indian IPR, Administrative
Machinery, Major international treaties signed by India,
Procedure for submitting patent and Enforcement of IPR at
national level etc. 07
03 Emerging Issues in IPR: Challenges for IP in digital economy,
e-commerce, human genome, biodiversity and traditional
knowledge etc. 05
04 Basics of Patents: Definition of Patents, Conditions of
patentability, Patentable and non -patentable inventions, Types
of patent appl ications (e.g. Patent of addition etc), Process
Patent and Product Patent, Precautions while patenting, Patent
specification Patent claims, Disclosures and non -disclosures,
Patent rights and infringement, Method of getting a patent 07
05 Patent Rules: Ind ian patent act, European scenario, US scenario,
Australia scenario, Japan scenario, Chinese scenario,
Multilateral treaties where India is a member (TRIPS
agreement, Paris convention etc.) 08
06 Procedure for Filing a Patent (National and International):
Legislation and Salient Features, Patent Search, Drafting and 07
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University of Mumbai B. E. (Biotechnology) Rev 2016 Page 167
Filing Patent Applications, Processing of patent, Patent
Litigation, Patent Publication etc, Time frame and cost, Patent
Licensing, Pa tent Infringement
Patent databases: Important websites, Searching international
databases
Assessment
Internal
Assessment consists of two tests which should be conducted at proper
intervals.
End Semester theory examination
Question paper will comprise of 6 questions each carrying 20 questions.
Total 4 questions need to be solved
Question no.1 will be compulsory based on entire syllabus wherein sub
questions can be asked.
Remaining questions will be randomly selected from all the modules
Weightage of ma rks should be proportional to number of hours assigned to
each module
References
1. Rajkumar S. Adukia, 2007, A Handbook on Laws Relating to Intellectual
Property Rights in India, The Institute of Chartered Accountants of India
2. Keayla B K, Patent system and related issues at a glance, Published by
National Working Group on Patent Laws
3. T Sengupta, 2011, Intellectual Property Law in India, Kluwer Law
International
4. Tzen Wong and Graham Dutfield, 2010, Intellectual Property and Human
Development: Current Trends a nd Future Scenario, Cambridge University
Press
5. Cornish, William Rodolph & Llewelyn, David. 2010, Intellectual Property:
Patents, Copyrights, Trade Marks and Allied Right, 7th Edition, Sweet &
Maxwell
6. Lous Harns, 2012, The enforcement of Intellectual Proper ty Rights: A Case
Book, 3rd Edition, WIPO
7. Prabhuddha Ganguli, 2012, Intellectual Property Rights, 1st Edition, TMH
8. R Radha Krishnan & S Balasubramanian, 2012, Intellectual Property Rights,
1st Edition, Excel Books
9. M Ashok Kumar and mohd Iqbal Ali, 2 -11, I ntellectual Property Rights, 2nd
Edition, Serial Publications
10. Kompal Bansal and Praishit Bansal, 2012, Fundamentals of IPR for Engineers,
1st Edition, BS Publications
11. Entrepreneurship Development and IPR Unit, BITS Pilani, 2007, A Manual on
Intellectual Pr operty Rights,
12. Mathew Y Maa, 2009, Fundamentals of Patenting and Licensing for Scientists
and Engineers, World Scientific Publishing Company
13. N S Rathore, S M Mathur, Priti Mathur, Anshul Rathi, IPR: Drafting,
Interpretation of Patent Specifications and Cl aims, New India Publishing
Agency
14. Vivien Irish, 2005, Intellectual Property Rights for Engineers, IET
15. Howard B Rockman, 2004, Intellectual Property Law for Engineers and
scientists, Wiley -IEEE Press
Page 168
University of Mumbai B. E. (Biotechnology) Rev 2016 Page 168
Course Code Course Name Credits
ILO8028 Institute Level Optional Subject II - Digital Business
Management 03
Objectives:
To familiarize with digital business concept
To acquaint with E -commerce
To give insights into E -business and its strategies
Outcomes:
The learner will be able to …..
Identify drivers of digital business
Illustrate various approaches and techniques for E -business and management
Prepare E -business plan
Module Detailed content Contact
Hours
1 Introduction to Digital Business -
Introduction, Background and current status, E -market places,
structures, mechanisms, economics and impacts. Difference
between physical economy and digital economy.
Drivers of digital business - Big Data & Analytics, Mobile,
Cloud Computing, Social media, BYOD, and Internet of Things
(digitally intelligent machines/s ervices). Opportunities and
Challenges in Digital Business, 09
2 Overview of E -Commerce
E-Commerce - Meaning, Retailing in e -commerce -products and
services, consumer behaviour , market research and
advertisement. B2B -E-commerce -selling and buying in private
e-markets, public B2B exchanges and support services, e -supply
chains, Collaborative Commerce, Intra business EC and
Corporate portals. The E-C models and applications, innovative
EC System -From E -government and learning to C2C, mobile
commerce and pervas ive computing. EC Strategy and
Implementation -EC strategy and global EC, Economics and
Justification of EC, Using Affiliate marketing to promote your e -
commerce business, Launching a successful online business and
EC project, Legal, Ethics and Societal imp acts of EC. 06
3 Digital Business Support services: ERP as e –business
backbone, knowledge Tope Apps, Information and referral
system
Application Development: Building Digital business
Applications and Infrastructure 06
4 Managing E -Business -Managing Knowledge, Management
skills for e -business, Managing Risks in e –business. Security
Threats to e -business -Security Overview, Electronic commerce
Threats, Encryption, Cryptography, Public Key and Private Key
Cryptography, Digital signatures, Digital Cert ificates, Security
Protocols over Public Networks: HTTP, SSL, Firewall as
Security Control, Public Key Infrastructure (PKI) for Security, 06
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University of Mumbai B. E. (Biotechnology) Rev 2016 Page 169
Prominent Cryptographic Applications
5 E-Business Strategy -E-business Strategic formulation - Analysis
of Company’s Internal and external environment, Selection of
strategy, E -business strategy into Action, challenges and E -
Transition(Process of Digital Transformation) 04
6 Materializing e -business: From Idea to Realization -Business
plan preparation.
Case Studies and presentations 08
Assessment
Internal
Assessment consists of two tests which should be conducted at proper
intervals.
End Semester theory examination
Question paper will comprise of 6 questions each carrying 20 questions.
Total 4 questions need to be solved
Question no.1 will be compulsory based on entire syllabus wherein sub
questions can be asked.
Remaining questions will be randomly selected from all the modules
Weightage of marks should be proportional to number of hou rs assigned to
each module
References
1. A textbook on E -commerce, Er Arunrajan Mishra, Dr W K Sarwade, Neha
Publishers & Distributors, 2011
2. E-commerce from vision to fulfilment, Elias M. Awad, PHI -Restricted, 2002
3. Digital Business and E -Commerce Management, 6th Ed, Dave Chaffey,
Pearson, August 2014
4. Introduction to E -business -Management and Strategy, Colin Combe,
ELSVIER, 2006
5. Digital Business Concepts and Strategy, Eloise Coupey, 2nd Edition, Pearson
6. Trend and Challenges in Digital Business Innovation, Vinoc enzo Morabito,
Springer
7. Digital Business Discourse Erika Darics, April 2015, Palgrave Macmillan
8. E-Governance -Challenges and Opportunities in : Proceedings in 2nd
International Conference theory and practice of Electronic Governance
9. Perspectives the Digital Enterprise –A framework for Transformation, TCS
consulting journal Vol.5
10. Measuring Digital Economy -A new perspective -
DOI: 10.1787/9789264221796 -en OECD Publishing
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University of Mumbai B. E. (Biotechnology) Rev 2016 Page 170
Course Code Course Name Credits
ILO8029 Institute Level Optional Subject II - Environmental
Management 03
Objectives:
Understand and identify environmental issues relevant to India and global
concerns
Learn concepts of ecology
Familiarise environment related legislations
Outcomes:
Learner will be able to…
Understand the concept of environmental management
Understand ecosystem and interdependence, food chain etc.
Understand and interpret environment related legislations
Module Detailed Contents Contact
Hours
01 Introduction and Definition of Environment: Significance of
Environment Management for contemporary managers, Career
opportunities.
Environmental issues relevant to India, Sustainable
Development, and The Energy scenario. 10
02 Global Environmental concerns : Global Warming, Acid Rain,
Ozone Depletion, Hazardous Wastes, Endangered life -species,
Loss of Biodiversity, Industrial/Man -made disasters,
Atomic/Biomedical hazards, etc. 06
03 Concepts of Ecology: Ecosystems and interdependence
between living organisms, habitats, limiting factors, carrying
capacity, food chain, etc. 05
04 Scope of Environment Management, Role & functions of
Government as a planning and regulating agency.
Environment Quality Manage ment and Corporate
Environmental Responsibility 10
05 Total Quality Environmental Management, ISO -14000, EMS
certification. 05
06 General overview of major legislations like Environment
Protection Act, Air (P & CP) Act, Water (P & CP) Act,
Wildlife Protection Act, Forest Act, Factories Act, etc. 03
Assessment
Internal
Assessment consists of two tests which should be conducted at proper
intervals.
End Semester theory examination
Question paper will comprise of 6 questions each carrying 20 questions.
Total 4 questions need to be solved
Question no.1 will be compulsory based on entire syllabus wherein sub
questions can be asked.
Remaining questions will be randomly selected from all the modules
Page 171
University of Mumbai B. E. (Biotechnology) Rev 2016 Page 171
Weightage of marks should be proportional to number of hours assigned to
each module
References
1. Environmental Management: Principles and Practice, C J Barrow, Routledge
Publishers London, 1999
2. A Handbook of Environmental Management Edited by Jon C. Lovett and
David G. Ockwell, Edward Elgar Publishing
3. Environm ental Management, T V Ramachandra and Vijay Kulkarni, TERI
Press
4. Indian Standard Environmental Management Systems — Requirements With
Guidance For Use, Bureau Of Indian Standards, February 2005
5. Environmental Management: An Indian Perspective, S N Chary and Vinod
Vyasulu, Maclillan India, 2000
6. Introduction to Environmental Management, Mary K Theodore and Louise
Theodore, CRC Press
7. Environment and Ecology, Majid Hussain, 3rd Ed. Access Publishing.2015
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University of Mumbai B. E. (Biotechnology) Rev 2016 Page 172
Course Code Course Name Credits
BTP801 Project -B 06
Guidelines:
Project groups: Groups of minimum two and not more than three students
can be made.
Students should spend considerable time in applying all the concepts
studied, into the Project, hence, eight hours each are allotted in project A, B
to the students.
Students are advised to take up industrial/ experimental/ simulation and/or
optimization based topics for their project
Students should report their guides weekly with their work.
Exam Guidelines
Term Wo rk - 100 Marks:
Presentation – 50 Marks
Report -50 Marks
Oral – 50 Marks
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University of Mumbai B. E. (Biotechnology) Rev 2016 Page 173
Course Code Course/Subject Name Credits
BTL801 Lab-VI 1.5
Concepts for experiments:
A minimum of 10 experiments must be performed from the following concept:
1. Physical property like pH, turbidity, conductivity, alkalinity determination of
waste water
2. Determination of total phosphorus content of waste water
3. Determination of total Kjeldahl Nitrogen of waste water
4. Determination of BOD of waste water
5. Determination of COD of waste water
6. Determination of Oil and grease content of waste water
7. Determination of total solids, total suspended solids and total dissolved solids
8. Determination of MLSS and MLVSS
9. Determination of Sludge Volume Index
10. Estimation of metals like iron, copper in waste water
11. Determination of chloride content of waste water
12. Estimation of coliform bacteria in waste water
13. Determination of phytoplankton in waste water
14. Determination of Most Probable Number of waste water
15. Removal of heavy metals by chemical methods from waste water Adsorption
Practical Examination
Duration for practical examination would be the same as assigned to the
respective lab per week.
A student becomes eligible for practical examination after comple ting a
minimum of eight out of ten experiments.
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University of Mumbai B. E. (Biotechnology) Rev 2016 Page 174
Course Code Course/Subject Name Credits
BTL802 Lab-VII 1.5
Concepts for experiments:
A minimum of 10 experiments must be performed from the following Concept:
Adsorption
Dialysis
Reverse Osmosis
Batch drying
Crystallization
Isolation and purification of biomolecules (protein/s or enzyme) from crude
source / fermentation broth
Determination of Solar Constants
Study of Enzyme inhibitors
Characterization of enzymes / Dete rmination of Molecular weight of
enzymes
Practical Examination
Duration for practical examination would be the same as assigned to the
respective lab per week.
A student becomes eligible for practical examination after completing a
minimum of eight out of ten experiments.