MSc Biotechnology Part II1 Syllabus Mumbai University

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2 M.Sc.
BIOTECHNOLOGY
SEMESTER- III
Course Code UNIT PTC and ATC Credits L / week
PSBT301 I Introduction to primary and secondary
metabolism, important pathways
leading to biosynthesis of secondary
metabolites in plants, Metabolic
products produced
from in vitro culturing of plant cells,
selection of plant cells/ tissues for
production of a specific products,
culture system in secondary plant
product,
Biosynthesis- batch, continuous
cultures, immobilized plant cell,
Biotransformation of precursors by
cell culturing, metabolic engineering
for production of secondary
metabolites, Hairy root culture,
elicitation 4 1
II Cryopreservation -Principle and types.
Germplasm conservation, Transgenic
plants-Edible vaccine, Golden rice 1
III Biology of cultured cells
Culture vessels, Culture Media,
Microbial contamination, cross
contamination.
Cryopreservation 1
IV Primary culture: Types, isolation of
tissues, culturing of different cells.
Cell lines: Development, Subculture
and propagation, immortalization of
cell line, cell line designation,
selection of cell lines, routine
maintenance, Cytotoxicity
Transformation
Culture of tumor cells 1
Course Code UNIT Medical microbiology Credits L / week
PSBT302 I Chromosomal disorders- 4 1

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3 Karyotyping, G banding,
Chromosome analysis, variations,
chromosome painting
II Infections of Respiratory tract-
Pneumonia,Tuberculosis.
Nosocomial- Psuedomonas.
Viral infections-HIV, Hepatitis.
Fungal-Candidiasis 1
III Molecular diagnostics for Pneumonia,
Tuberculosis, Pseudomonas, HIV,
Hepatitis. Candidiasis 1
IV Biofilms in medicine 1
Course Code UNIT Clinical Studies Credits L / week
PSBT303 I
4 1
II 1
III 1
IV 1
Course Code UNIT Developmental Biology Credits L / week
PSBT304 I Human Embryonic development:
Events during fertilization, in-vitro
fertilization, Zonapellucidaa,
glycoprotein, Oelemma protein and
their role in fertilization, sperm 4 1 /FX
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4 antigens and their functional
significance. Molecular and
biochemical events during sperm
function
II Post fertilization events: early
embryonic development, establishing
multi-cellularity, formation of
blastula, embryonic germ layer,
tracking of migrating cells. 1
III Molecular mechanism of sex hormone
action and regulation of gene
expression. Implantation and
endometrium antigens involved in
implantation. Immunology of
pregnancy. Superovulation, embryo
culture and embryo transfer
technology. 1
IV Infertility and reproductive vaccines.
Frontiers in contraceptive research.
Cryopreservation of sex gametes and
embryos. Ethical issues related to
embryo research. 1
PSBTP301 PTC and ATC 2 4
PSBTP302 Medical microbiology 2 4
PSBTP303 Clinical Studies 2 4
PSBTP304 Developmental Biology 2 4

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5 Practicals for Semester III
Sr No. Experiment
1 PTC Media preparation
Seed sterilization ,
Callus induction
Isolation and induction of anther cultures using hibiscus
Protoplast isolation
Soma tic embryogenesis
2 ATC
Trypsinization , Staining of animal cells
-Monolayer formation (fibroblast)
To assay the radical scavenging activity of a tissue hydrolysate -DPPH
method
3 Toxicology - MTT assay
4
5 Medical diagnostic – Iden tificati on of or ganis ms fro m s p e c i m ens
(Multiple drug resistant S. aureus, Pseudomonas spps,
Klebsiella pneumoniae, E. coli ). Staining of Biofilms
6
7.'eYelRSmental %iRlRg\ 9isit tR laERratRr\/ video lectures for latest
develo pments in the field. To b e documented Study and present a published clinical case report
Candling, Observing chick embryo- stages of deve lopment;
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6 SEMESTER IV
Cour se Code UNIT Nanotechnology Credits L / Week
PSBT401 I Introduction, synthesis of
nanomaterials, biological methods,
use of microbial system & plant
extracts, use of proteins &
templates like DNA.
Characterization of nanomaterials,
analysis techniques, properties of
nanomechanical, optical,
magnetic properties, electrical
conductivity, thermal
conductivity.
4 1
II Carbon nanotubes
Nanorobotiocs devices of nature:
ATP synthese, the kinen, myosin,
dynein, flagella modulated motion.1
III Nanomedicine: biopharmaceutics,
implantable materials, implantable
chemicals, surgical aids,
diagnostic tools, nanosensors,
nano scanning, nano enabled drug
delivery system, nanorobotics in
medicine. 1
IV Application of nanomaterials in
food,cosmetics, agriculture,
environment management 1
Course Code UNIT GMO and environment Credits L / Week
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7 II 1
III Solid waste treatment,
pollution indicators & biosensors
biodegradation of xenobiotics,
pesticides, phytoremediation 1
IV Biodegration of waste from f o o d ,
textile, petrochem, paper
industries, biological
detoxification,
Removal of oil spillage & grease
deposits 1
Course Code UNIT Bioinformatics Credits L / Week
PSBT403 I Organization of biological data,
databases (raw and processed),
Quering in data bases.
Primers in biology(Designing of
primers, kinds of primers)
4 1
II Gene finding, motif finding and
multiple sequence alignment.
Protein sequence analysis (theory
and algorithms)
Protein structure analysis and
applications. 1
III Gene expression profiling and its
applications.
Microarray technology and basics.
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8 organization of data
Human genome analysis
IV Proteomics.
Exploration of data bases, retrieval
of desired data, BLAST etc.
Gene clusters and fusions,
consensus sequences, exon intron
finder, sequence logo. 1
Course Code UNIT Biostatistics Credits L / Week
PSBT404 I Statistical population, sample from
population, Random sample.
Central Tendency: Mean, Median
and Mode, Standard Deviation
Confidence intervals
4 1
II Gaussian Distribution and testing
for normality, Non-parametric
tests (Sign test, Wilcoxon test,
Mann-Whitney Test, Krushkal-
Whllis test,), transforming data to
create Gaussian Distribution 1
III Test of Significance.
Hypothesis testing:- Theory of
errors- Type I and Type II errors,
Null hypothesis, P values-one v/s
two tail P values, t-test(paired &
unpaired), z-test, Chi square test,
contingency table. 1
IV Comparing three or more groups-
Introduction to ANOVA, One way
ANOVA, repeated measures
ANOVA, Friedman Test.
Correlation and Regression: Linear
and multiple Correlation and
Regression. 1

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9 Semester IV Practicals
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Sr No. Experiment
1 Multiple alignment - Phylogenetic tree
2 BLAST - orthologs and paralogs , homologs
3 Motif finding
4 KEGG
5 Structure of proteins - identificatio n of chains helices, special groups,
metal ions etc. CATH / SCOP classification of a given protein
6
7 Bioremed iation- isolatio n of metal tolerant o r ganism s & study
their growth characteristics and pattern
8 Composting – physical & chemical parameters
PSBTP401 Nanotechnology 2 4
PSBTP402 *02 and environment 2 4
PSBTP403 Bioinformatics 2 4
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10 References  
Sr No Title  of the Book   Author    Publisher   
1 A Introduction  to Biostatistics  (Second  
Edition ‐2005)  N. Gurumani M J P Publishers
2 Basic  Biostatistics  (2008)   B. Burt Gerstman Jones  and Bartlett  
Publishers  
3 Biostatistics:  Afoundation  For Analysis  In 
Health  Sciences  ( 7th Edition  1999)   Wayne  W. Daniel  John  Wiley  & Sons  Inc. 
4 Fundamentals  of Biostatistics  (2006)   Veer  BalaRastogi   Ane Books  India  
5 Biostatistics ‐ The Bare  Essentials  ( Second  
Edition  2000)  NosmanStreiner B. C. Decker  Inc.
6 Computer  Based  Decision  Making  in 
Medicine  E. A. Shortifile American  Elsevier
7  Bioinformatics  : Sequence  and Genome  
Analysis  (Second  Edition  2004)  David  W. Mount   ColdspringHarbor  
Laboratory  Press  
8 Bioinformatics  and Functional  Genomics  ( 
2003  ) Jonathan  Pevsner   John  Wiley  & Sons  
Publications  
Sr No  Title  of the Book   Author   Publisher  
1 Plant  Cells  in liquid  culture  (1991)   Payne  Shuler   Hanser  Publishers  
2 Culture  of Animal  Cells  : A Manual  Of Basic  
Techniques  ( 4TH Edition,  2000)  R. Ian Freshney   Wiley ‐Liss 
3 Principles  and Practice  of Animal  Tissue  
Culture  (2007)  SudhaGangal   Universities  Press  
4 Langman’s  Medical  Embryology ( 9thEdition  
2004)  T. W. Sadler. Lippincott  Williams  & 
Wilkins  
5 Essential  Developemental  Biology  (2nd
Edition  2006)  J. M. W. Slack Blackwell  Publishing  

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11 6 Developemental  Biology  (8th Edition  2006)  Scott  F. Gilbert   Sinauer  Associates,  Inc. 
7 The Nanoscopeencyclopedia  of 
nanoscience  and nanochehnology,  Vol. I 
(2005)  Dr.ParagDiwan  and 
AshishBharadwaj  Pentagon  Press  New  
Delhi  
8 The Nanoscopeencyclopedia  of 
nanoscience  and nanochehnology,  Vol V 
(2005)  Dr.ParagDiwan  and 
AshishBharadwaj  Pentagon  Press  New  
Delhi  
9 The Nanoscopeencyclopedia  of 
nanoscience  and nanochehnology,  Vol VI 
(2005)  Dr.ParagDiwan  and 
AshishBharadwaj  Pentagon  Press  New  
Delhi  
10 Nano  forms  of carbon  and its applications  
(2007)  Prof.Maheshwar  
Sharon  and Dr.Madhuri  
Sharon  Manad  Nanotech  Pvt. 
Ltd. 
11 Biotechnanotechnology  lessons  from  
Nature  (2004)  David  Goodsell Wiley ‐Liss A John  Wiley  
and sons  
12 Nanotechnology ‐ Basic  science  and 
emerging  technologies  (2005)  WillsonKannangava,  
Smith,  Simmons,  
Raguse  Oversease  Press  
13 Texbook  of Biotechnology  (2005)   R. C. Dubey   S. Chand  and Co. 
14 Nanotechnology ‐ Principles  and practices  S. K. Kulkarni   Capital  Publishing  Co. 
Sr No  Title  of the Book   Author   Publisher  
1 
2 
3 
4 
5 
6 
7 
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