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2 M.Sc.
BIOTECHNOLOGY
SEMESTER- III
Course Code UNIT PTC and ATC Credits L / week
PSBT301 I Introduction to primary and secondary
metabolism, important pathways
leading to biosynthesis of secondary
metabolites in plants, Metabolic
products produced
from in vitro culturing of plant cells,
selection of plant cells/ tissues for
production of a specific products,
culture system in secondary plant
product,
Biosynthesis- batch, continuous
cultures, immobilized plant cell,
Biotransformation of precursors by
cell culturing, metabolic engineering
for production of secondary
metabolites, Hairy root culture,
elicitation 4 1
II Cryopreservation -Principle and types.
Germplasm conservation, Transgenic
plants-Edible vaccine, Golden rice 1
III Biology of cultured cells
Culture vessels, Culture Media,
Microbial contamination, cross
contamination.
Cryopreservation 1
IV Primary culture: Types, isolation of
tissues, culturing of different cells.
Cell lines: Development, Subculture
and propagation, immortalization of
cell line, cell line designation,
selection of cell lines, routine
maintenance, Cytotoxicity
Transformation
Culture of tumor cells 1
Course Code UNIT Medical microbiology Credits L / week
PSBT302 I Chromosomal disorders- 4 1
Page 3
3 Karyotyping, G banding,
Chromosome analysis, variations,
chromosome painting
II Infections of Respiratory tract-
Pneumonia,Tuberculosis.
Nosocomial- Psuedomonas.
Viral infections-HIV, Hepatitis.
Fungal-Candidiasis 1
III Molecular diagnostics for Pneumonia,
Tuberculosis, Pseudomonas, HIV,
Hepatitis. Candidiasis 1
IV Biofilms in medicine 1
Course Code UNIT Clinical Studies Credits L / week
PSBT303 I
4 1
II 1
III 1
IV 1
Course Code UNIT Developmental Biology Credits L / week
PSBT304 I Human Embryonic development:
Events during fertilization, in-vitro
fertilization, Zonapellucidaa,
glycoprotein, Oelemma protein and
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4 antigens and their functional
significance. Molecular and
biochemical events during sperm
function
II Post fertilization events: early
embryonic development, establishing
multi-cellularity, formation of
blastula, embryonic germ layer,
tracking of migrating cells. 1
III Molecular mechanism of sex hormone
action and regulation of gene
expression. Implantation and
endometrium antigens involved in
implantation. Immunology of
pregnancy. Superovulation, embryo
culture and embryo transfer
technology. 1
IV Infertility and reproductive vaccines.
Frontiers in contraceptive research.
Cryopreservation of sex gametes and
embryos. Ethical issues related to
embryo research. 1
PSBTP301 PTC and ATC 2 4
PSBTP302 Medical microbiology 2 4
PSBTP303 Clinical Studies 2 4
PSBTP304 Developmental Biology 2 4
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5 Practicals for Semester III
Sr No. Experiment
1 PTC Media preparation
Seed sterilization ,
Callus induction
Isolation and induction of anther cultures using hibiscus
Protoplast isolation
Soma tic embryogenesis
2 ATC
Trypsinization , Staining of animal cells
-Monolayer formation (fibroblast)
To assay the radical scavenging activity of a tissue hydrolysate -DPPH
method
3 Toxicology - MTT assay
4
5 Medical diagnostic – Iden tificati on of or ganis ms fro m s p e c i m ens
(Multiple drug resistant S. aureus, Pseudomonas spps,
Klebsiella pneumoniae, E. coli ). Staining of Biofilms
6
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6 SEMESTER IV
Cour se Code UNIT Nanotechnology Credits L / Week
PSBT401 I Introduction, synthesis of
nanomaterials, biological methods,
use of microbial system & plant
extracts, use of proteins &
templates like DNA.
Characterization of nanomaterials,
analysis techniques, properties of
nanomechanical, optical,
magnetic properties, electrical
conductivity, thermal
conductivity.
4 1
II Carbon nanotubes
Nanorobotiocs devices of nature:
ATP synthese, the kinen, myosin,
dynein, flagella modulated motion.1
III Nanomedicine: biopharmaceutics,
implantable materials, implantable
chemicals, surgical aids,
diagnostic tools, nanosensors,
nano scanning, nano enabled drug
delivery system, nanorobotics in
medicine. 1
IV Application of nanomaterials in
food,cosmetics, agriculture,
environment management 1
Course Code UNIT GMO and environment Credits L / Week
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7 II 1
III Solid waste treatment,
pollution indicators & biosensors
biodegradation of xenobiotics,
pesticides, phytoremediation 1
IV Biodegration of waste from f o o d ,
textile, petrochem, paper
industries, biological
detoxification,
Removal of oil spillage & grease
deposits 1
Course Code UNIT Bioinformatics Credits L / Week
PSBT403 I Organization of biological data,
databases (raw and processed),
Quering in data bases.
Primers in biology(Designing of
primers, kinds of primers)
4 1
II Gene finding, motif finding and
multiple sequence alignment.
Protein sequence analysis (theory
and algorithms)
Protein structure analysis and
applications. 1
III Gene expression profiling and its
applications.
Microarray technology and basics.
Microarray analysis and 1 "CPVU *OEJBO (.0 3FTFBSDI
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8 organization of data
Human genome analysis
IV Proteomics.
Exploration of data bases, retrieval
of desired data, BLAST etc.
Gene clusters and fusions,
consensus sequences, exon intron
finder, sequence logo. 1
Course Code UNIT Biostatistics Credits L / Week
PSBT404 I Statistical population, sample from
population, Random sample.
Central Tendency: Mean, Median
and Mode, Standard Deviation
Confidence intervals
4 1
II Gaussian Distribution and testing
for normality, Non-parametric
tests (Sign test, Wilcoxon test,
Mann-Whitney Test, Krushkal-
Whllis test,), transforming data to
create Gaussian Distribution 1
III Test of Significance.
Hypothesis testing:- Theory of
errors- Type I and Type II errors,
Null hypothesis, P values-one v/s
two tail P values, t-test(paired &
unpaired), z-test, Chi square test,
contingency table. 1
IV Comparing three or more groups-
Introduction to ANOVA, One way
ANOVA, repeated measures
ANOVA, Friedman Test.
Correlation and Regression: Linear
and multiple Correlation and
Regression. 1
Page 9
9 Semester IV Practicals
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Sr No. Experiment
1 Multiple alignment - Phylogenetic tree
2 BLAST - orthologs and paralogs , homologs
3 Motif finding
4 KEGG
5 Structure of proteins - identificatio n of chains helices, special groups,
metal ions etc. CATH / SCOP classification of a given protein
6
7 Bioremed iation- isolatio n of metal tolerant o r ganism s & study
their growth characteristics and pattern
8 Composting – physical & chemical parameters
PSBTP401 Nanotechnology 2 4
PSBTP402 *02 and environment 2 4
PSBTP403 Bioinformatics 2 4
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Page 10
10 References
Sr No Title of the Book Author Publisher
1 A Introduction to Biostatistics (Second
Edition ‐2005) N. Gurumani M J P Publishers
2 Basic Biostatistics (2008) B. Burt Gerstman Jones and Bartlett
Publishers
3 Biostatistics: Afoundation For Analysis In
Health Sciences ( 7th Edition 1999) Wayne W. Daniel John Wiley & Sons Inc.
4 Fundamentals of Biostatistics (2006) Veer BalaRastogi Ane Books India
5 Biostatistics ‐ The Bare Essentials ( Second
Edition 2000) NosmanStreiner B. C. Decker Inc.
6 Computer Based Decision Making in
Medicine E. A. Shortifile American Elsevier
7 Bioinformatics : Sequence and Genome
Analysis (Second Edition 2004) David W. Mount ColdspringHarbor
Laboratory Press
8 Bioinformatics and Functional Genomics (
2003 ) Jonathan Pevsner John Wiley & Sons
Publications
Sr No Title of the Book Author Publisher
1 Plant Cells in liquid culture (1991) Payne Shuler Hanser Publishers
2 Culture of Animal Cells : A Manual Of Basic
Techniques ( 4TH Edition, 2000) R. Ian Freshney Wiley ‐Liss
3 Principles and Practice of Animal Tissue
Culture (2007) SudhaGangal Universities Press
4 Langman’s Medical Embryology ( 9thEdition
2004) T. W. Sadler. Lippincott Williams &
Wilkins
5 Essential Developemental Biology (2nd
Edition 2006) J. M. W. Slack Blackwell Publishing
Page 11
11 6 Developemental Biology (8th Edition 2006) Scott F. Gilbert Sinauer Associates, Inc.
7 The Nanoscopeencyclopedia of
nanoscience and nanochehnology, Vol. I
(2005) Dr.ParagDiwan and
AshishBharadwaj Pentagon Press New
Delhi
8 The Nanoscopeencyclopedia of
nanoscience and nanochehnology, Vol V
(2005) Dr.ParagDiwan and
AshishBharadwaj Pentagon Press New
Delhi
9 The Nanoscopeencyclopedia of
nanoscience and nanochehnology, Vol VI
(2005) Dr.ParagDiwan and
AshishBharadwaj Pentagon Press New
Delhi
10 Nano forms of carbon and its applications
(2007) Prof.Maheshwar
Sharon and Dr.Madhuri
Sharon Manad Nanotech Pvt.
Ltd.
11 Biotechnanotechnology lessons from
Nature (2004) David Goodsell Wiley ‐Liss A John Wiley
and sons
12 Nanotechnology ‐ Basic science and
emerging technologies (2005) WillsonKannangava,
Smith, Simmons,
Raguse Oversease Press
13 Texbook of Biotechnology (2005) R. C. Dubey S. Chand and Co.
14 Nanotechnology ‐ Principles and practices S. K. Kulkarni Capital Publishing Co.
Sr No Title of the Book Author Publisher
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