UNIVERSITY OF MUMBAI
Syllabus for the M.Sc. Semester I and
Semester II
Program: M.Sc.
Course: Bio -Chemistry
(Credit Based Semester and Grading System with
effect from the academic year 2017–2018 )
SYLLABUS OF M.Sc. CREDIT SYSTEM IN BIOCHEMISTRY
TO BE BROUGHT INTO FO RCE FROM THE ACADEMIC YEARS 2017 -2018
Objectives
1. To Strengthen the base in fundamental aspects of Biochemistry viz. Bio -organic
and Biophysical Chemistry, Instrumentation, Metabolism, Med ical/Clinical
Chemistry and Human Nutrition and Dietetics.
2. To introduce soft skills development component to create awareness and develop
competence in personality development, communication skills, academic and
professional skills. Empower the students wi th leadership qualities,
entrepreneurship and start -ups for employment, stress & time management.
3. To develop broad perspective with respect to the advanced areas of Biochemistry
Viz. Genetics and Molecular Biology, Immunology, Recombinant DNA
Techniques and Industrial Biochemistry.
4. To familiarize with research methodology to help students develop research
aptitude through research projects.
5. To create awareness in Biostatistics for app licability to research .
6. To open up new vistas in the fast emerging and developing areas of Bio -
informatics and applications of computers in Bio -chemistry .
7. To sharpen practical skills in performing experiments involving latest protocols .
8. To augment experime ntal expertise by handling modern instruments .
9. To train young minds for gainful employment in industry, research -oriented career
and qualifying examinations like NET and SET .
10. To develop scientific temper and interest by exposure through Interne t.
Computers , various data bases and industrial visits and study/educational tours .
11. To provide platform for interaction with scientist s at research centres/ universities/
industries including internship for training/ summer project for 03 to 06 months
and also for the gainful employment.
Scheme for Theory Paper
(4 Credits per Paper per Semester)
M.Sc. Semester I
Course Code Topic Headings Credits
PSBCH -101 Advanced Bio -organic Chemistry 4
PSBCH -102 Advanced Instrumentation and Analytical Techniques 4
PSBCH -103 Industrial Biochemistry and Bioinformatics 4
PSBCH -104 Research Methodology, Bio -statistics & Soft Skills
Development 4
M.Sc. Semester II
Course Code Topic Headings Credits
PSBCH -201 Advanced Bio -organic Chemistry 4
PSBCH -202 Advanced Instrumentation and Analytical Techniques 4
PSBCH -203 Industrial Biochemistry and Bioinformatics 4
PSBCH -204 Research Methodology, Bio -statistics & Soft skills
Development 4
M.Sc. Semester III
Course Code Topic Headings Credits
PSBCH -301 Advanced Genetics 4
PSBCH -302 Advanced Immunology 4
PSBCH -303 Advanced Metabolism 4
PSBCH -304 Clinical and Pharmaceutical Biochemistry, Human Nutrition
and Dietetics 4
M.Sc. Semester IV
Course Code Topic Headings Credits
PSBCH -401 Advanced Genetics 4
PSBCH -402 Advanced Immunology 4
PSBCH -403 Advanced Metabolism 4
PSBCH -404 Clinical and Pharmaceutical Biochemistry, Human Nutrition 4
and Dietetics
Semester -wise Details of Unit I to IV in each theory paper
SEMESTER I
Course Code UNIT TOPIC HEADINGS Credits L /
Week
PSBCH101 I Biochemical Basic of Evolution
4 1
II Bioenergetics 1
III Protein chemistry& Enzymology 1
IV Membrane Biochemistry & Cell
Signalling 1
PSBCH102 I Colligative properties
4 1
II Acids, Bases & Buffers 1
III Microscopy and Radioactive
Techniques 1
IV Spectroscopic Techniques 1
PSBCH103 I Bioprocess Technology &
Fermentation
4 1
II Technologies in cell & Tissue
culture & Marine Biotechnology 1
III Techniques in Food Preservation 1
IV Bioinformatics – I 1
PSBCH 104 I Research and Research Design
4 1
II Presentation and Processing of Data 1
III Analysis of Data and Sampling
Techniques 1
IV Soft Skills Development - I 1
PSBCHP 101 Colorimetry, Volumetry, Enzymology,
Buffers, Microscopy 2 4
PSBCHP 102 Biochemical, Clinical Analysis 2 4
PSBCHP 103 Bioinformatics - 2 2 4
PSBCHP 104 Research Methodology, Biostatistics ,Soft 2 4
Skills Development -1
SEMESTER II
Course Code UNIT TOPIC HEADINGS Credits L / Week
PSBCH201 I Plant Biochemistry
4 1
II Endocrinology 1
III Biochemistry of Tissues 1
IV Bioluminescence, Unusual
Biomolecules &Natural Bioactive
Compounds 1
PSBCH202 I Centrifugation
4 1
II Chromatography
III Electrophoresis & Sequencing
Techniques
IV Special Instrumental Methods of
Analysis
PSBCH203 I Carbohydrates , Proteins and
Lipids of Industrial Importance
4 1
II Environmental Biotechnology 1
III Nanotechnology and other topics 1
IV Bioinformatics - II 1
PSBCH204 I Report writing & Presentation
4 1
II Estimation and testing of
Hypothesis 1
III Non-parametric tests, Diagnostic
Tests & Vital Statistics. 1
IV Soft Skills Development - II
PSBCHP 201 Chromatography and electrophoresis
techniques. 2 4
PSBCHP 202 Clinical Estimations 2 4
PSBCHP 203 Extraction, Isolation And Partial Purification
Techniques 2 4
PSBCHP 204 Biostatistics & Bioinformatics 2 4
Detail Theory Syllabus
Semester I
Course Code Title Credits
PSBCH101 Advanced Bioorganic Chemistry 4
Unit I:
1.1 Biochemical Basis of Evolution
1.1.1 Theories of Evolution – Time scale and spontaneous origin
of life.
1.1.2 Genesis of oxygen generating photosynthesis and aerobic
respiration. Methanogens – evolution of prokaryotes,protists &
eukaryotes
1.1.3 Opar in’s Hypothesis, Miller Experiment, Smith’s Model, RNA first
model . Theories regarding origin of mitochondria and chloroplast
1.1.4 Evolution of proteins and nucleic acid – elastic analysis. Evolution of
introns. Evolutionary view of exon domain relationships .
1.1.5 Process or Origin of life of Eu karyo tes, Molecular Evolution
of Proteins.
Number
of
Lectures
15
Unit II:
2.1 Bio-energetics
2.1.1 Chemistry of Water . Laws of thermodynamics as applied to
biological systems, enthalpy, entropy, free energy, standard free
energy
2.1.2 Role of High Energy phosphates in Bio -energetics and energy
capture, Theories of ATP Biosynthesis
Electron Transport Chain in Plants, Eukaryotes and Prokaryotes,
Significance or Redox potentials, Mechanism of Oxidative
Phosphorylation. Uncouplers and Inhibitors of energy transfer.
2.1.3 Numerical problems based on the above
15
Unit: III
3.1 Protein Chemistry
3.1.1 Polypeptide backbone, covalent and non -covalent interactions,
end-group analysis by chemical and enzymatic methods, Conformation,
Configuration
3.1.2 Details of 10, 20 , 30and 40structures, problems based on
determination of 10structure, Ramachandran Plot, structure -function
relation of protein (Ex. Haemoglobin) Protein -Protein
interaction (actin, tubulin),Leucine zipper, Zinc finger,
trans -membrane regions,
3.1.3 Chemical modification and cross -linking in proteins, dynamic properties
and mechanisms of protein folding. Domains , motifs, and folds in
protein structure . Prion proteins, prion domains. Their role in
neurodegenerative disease.
3.2 Enzymology
3.2.1 IUB/EC Enzymes classification active site identification and
Conformation
3.2.2 Thermodynamics of catalysis, energy activation, relation of ∆G
and K eq. Coupled reactions (endergonic and exergonic) in biochemical
pathways
3.2.3 Michaelis -Menten Kinetics of monosubstrate enzyme
reaction, LB Plot, EinsethalCornish Bowden Plots
3.2.4 Mechanism of Enzyme Action for Acid –Base Electrostatic
andCovalent Catalysis (Ex. Chymotrypsin, Carboxypeptidase -A),
factors affecting catalysis. Metal, co -factor, and co -enzyme requirements
3.2.5 Enzyme Inhibition -Reversible competitive, non -competitive,
uncompetitive, Partial, Mixed, Allosteric Irreversible and Feedback
Inhibition . Enzyme inhibitors as drugs
3.2.6 Allosteric Enzymes -Kinetics Significance of Sigmoidal Behaviour, Role
in Metabolic Regulation.
3.2.7 Iso-enzymes – separation and significance
3.2.8 Enzyme Reaction in non -aqueous Media.
3.2.9 Clinical Enzymology - Enzymes as therapeutic agents, diagnostic tools
and laboratory agents.
06
09
Unit: IV
4.1 Membrane Biochemistry
4.1.1 Biological membrane; structure and assembly: constituents, bacterial cell
envelop, asymmetry flip flop, protein lipid interaction, factors affecting physical
properties of membranes.
4.1.2 Molecular constituents of Membrane and supra -moleculararchitecture .
Biological and physical membrane models. Specialized features like lipid rafts,
caveolae andtight junctions
4.1.3 Principles and Mechanism of Diffusion and Passive, Active & facilitated
Transport . Endocytosis, exocytosis .
4.1.4 Specialized mechanism for transport of macromolecules, gap junctions, nuclear
pores, toxins, control of transport processes, binding proteins, hormone effects
.
4.1.5 Role of Na, K ATPase and the passive permeability of the plasma membrane to
Na, K and Cl, voltage and ligand gated ion channels, ATP -ADP exchanger.
Molecular mechanisms, ion translocating antibiotics , valinomycin, gramicidin,
ouabain, group translocation, ionophores , electrical gradient, energy coupling
mechanism. Disorders result from abnormalities of membrane structure and
Functions Familial hypercholesterolemia, cystic fibrosis, hereditary
Spherocytosis
4.1.6 Artificial Membranes (Liposomes) in Drug Delivery, Kinetics of Super-molecular
Membrane Assembly -Viruses and Ribosomes .
4.2 Cell Signalling
4.2.1 Classes of Cell Receptors, Molecular Mechanism of Cell Signalling via G -protein
linked Cell Surface Receptors. Signaling molecules and their receptors Modes of
cell-cell signaling (endocrine, paracrineand autocrine) Steroid hormones, thyroid
hormones, vit D3 and retinoic acid Steroid superfamily receptors and their
functions. Nitric oxide, neurotransmitter, peptide hormones, growth factors,
Eicosanoids, plant hormones.
4.2.1 Trimeric G -proteins and their regulatory mechanism, Role of Ca++ as an
intracellular signal, Ca++ / Calmodulin dependent protein kinase, cAMP - Ca++
Pathway
4.2.2 Pathways of intracellular signal transduction cAMP, cGMP, Phospholipid and
Ca, Ras, Raf and MAP kinase pathways JAK/STAT pathway
4.2.3 Signal transduction and cytoskeleton Integrin and signal transduction , regulation
ofActin Cytoskeleton Signaling indevelopment and differentiation using
following examples Mesoderm. Induction in xenopus and Eye development in
Drosophila
4.2.4 Programmed Cell Death (apoptosis) involving onco -genes and tumour
suppressor genes
07
08
Course Code Title Credits
PSBCH102 Advanced Instrumentation and Analytical Techniques 4
Unit I:
1.1 Colligative Properties
1.1.1 Definitions, Factors affecting and Physiological Applications of
Osmosis, Measurement of osmotic pressure,
Osmoregulation ,Adsorption, Colloids, Surface Tension and
Viscosity
1.1.2 Numerical Problems based on above concepts Number
of
Lectures
15
Unit II:
2.1. Acid, Bases and Buffers
2.1.1 Ionization, Dissociation, Acidity, Basicity theories of Acid and
Bases, Strength of Acids and Bases, Acid -Base Equilibrium in
Aqueous and Non -aqueous media.
2.1.2 pH, pH -dependent functions and structures off bio -molecules,
Henderson –Hasselbach Equation, Different methods for
measurement of pH. Use of Indicators, Buffers, Amino Acid
titrations, Formal Titration .Biologically important buffers,
Buffering of blood.
2.1.3 Numerical Problems based on above concepts
15
Unit: III
3.1 Microscopy
3.1.1 Basic principles, instrumentation and application of Phase,
ultraviolet and interference microscope and Fluorescence
microscopy
3.1.2 Electron microscope – scanning emission microscopy, transmission
emission microscopy
3.1.3 Confocal microscopy and Atomic force microscopy
3.1 Radioisotope Techniques
3.2.1 Nature of radioactivity & its detection and measurements of
Radioactivity , Radioactive decay, Interaction of radioactivity with
matter GM Counter, Scintillation Counter, Advantages and
Disadvantages of Scintillation Counting Pulse Height Analyser.
3.2.2 Isotope Dilution, Analysis, Autoradiography, Application of
Radioisotopes in Biological Science
3.2.3 Safety M easures in Handling Isotopes.
07
08
Unit: IV
4.1 Spectroscopic Techniques
4.1.1 Beer -Lamberts Law, Its verifications and Deviations, Concept of
Absorptions, Transmission, Scattering, Phosphorescence,
Fluorescence, Luminescence, Diffraction Spectra .
Infrared Spectra of common functional groups.
4.1.2 Principle Instrumentation, working and application of – U V,
Visible and IR Spectroscopy, Disadvantages of IR spectroscopy,
Turbidometry and Nephlometry.
4.1.3 Principle, instrumentation, working and application of –
Spectrofluorometric , Flame Spectrophotometry, Atomic
AbsorptionSpectrometry, Luminometry. Fluorescence spectra and
the study of protein structure.
4.1.4 Principle, instrumentation, working and application of - Nuclear
Magnetic Resonance(NMR), Electron Spin Resonance (ESR),
Mossbauer Spectroscopy, Matrix Assisted LASER Desorption,
ionization, Time of Flight -Mass Spectroscopy (MALDI -TOF -MS)
4.1.5 X-Ray Diff raction Spectra, Optical Rotatory Dispersion, (ORD),
Circular Dichroism (CD)
4.1.6 LASER - Principle, applications in Medicine and Biological
Sciences
15
Course Code Title Credits
PSBCH103 Industrial Biochemistry and Bioinformatics - 1 4
Unit I: Bio Process Technology and Fermentation
1.1 Bioprocess Technology
1.1.1 Types of Bioreactors - Stirred Tank, Recycle reactors,
discontinuous, semi continuous and continuous.
1.1.2 Parameters for Bio process – Bio mass, Substrates, product, O 2
and CO 2, Temperature, agitation.
1.1.3 Bio process monitoring with respect to O 2 transfer, energy
transfer, rate of utilization, efficiency and computer base
monitoring
1.1.4 Downstream processing, process for product recovery, recycling
of residual raw, by product recovery, waste/ effluent treatment
1.2 Fermentation
1.2.1 Primary and secondary of microbes, inoculums preparation,
fermentation media, industrial sterilization, strain improvement, Number of
Lectures
08
07
metabolic and genetic regulations during fermentations, pure and
mix culture fermentations.
1.2.2 Products from microorganisms – enzymes (Amylases, Proteases,
Pectinases), Primary metabolites (Glu, vit B12) , Antibiotics
(Penicillin), Beverages (wine, Beer)
1.2.3 Fuels from microbes, microbial polymers and microbial steroid
bio transformations.
Unit II: Technologies in Cell and Tissue Cultures and Marine Bio
Technology .
2.1 Plant Tissue Culture (PTC)
2.1.1 Principles, Techniques, Methodology and Application of PTC
2.1.2 Micropropogation and Protoplast fusion
2.1.3 Suspension Cultures for production and secondary metabolites
2.1.4 Use of PTC in production of transgenics.
2.2 Animal Tissue Culture (ATC)
2.2.1 Principles, Techniques, Methodology and Application of ATC
2.2.2 Transfection using eggs, cultured stem cells and nuclei in
development of transgenic animals.
2.2.3 Frontiers of contraceptive research, cryopreservation of sex
gametes& embryos, Eth ical issues in embryo research.
2.3 Microbial Tissue Culture (MTC)
2.3.1 Principles, Techniques, Methodology and Application of MTC
2.3.2 Commercial production of industrially important microbial
strains, role of ATCC and microbial cell banks.
2.3.3 Microbes as products, Single Cell Protein (SCP) and Yeast
(nutrient)
2.3.4 Bioremediation, Oil spills, Degradation of waste water,
Chemicals and heavy metals, microbial leaching (Cu, Zn, Fe,
Ag, Mn, Hg, As, Sb)
05
05
05
Unit: III Techniques in Food preservation
3.1 Bio Chemistry of Food Spoilage
3.1.1 Factors causing food spoilage during food ripening, vegetable
maturation and their control.
3.1.2 Post mortem changes in meat and their control.
3.2 Food Preservation
3.2.1 General principles of food preservation
3.2.2 Preservation by use of high and low temperatures, drying,
radiations, chemical preservatives, inert gases, mechanical
preservation techniques (vacuum packaging, tetra packs).
05
05
3.3 QC, GMP and other topics
3.3.1 General princip les of Quality Control and Good Manufacturing
Practices in food industry.
3.3.2 Determination of shelf – life of food products, transport of
perishable food items.
3.3.3 Food Adulteration – Common food adulterants, their harmful
effects and physical and chemical methods for their detection.
3.3.4 Role of ISI Agmark and FDA in food industry.
05
Unit: IV Bioinformatics - I
4.1 Introduction to Bioinformatics
4.1.1 Central Dogma of Molecular Biology
4.1.2 Human Genome Project - Ethical, legal and social issues
4.1.3 Bioinformatics - Need and applications on various fields of Biology
4.1.4Introduction to Next -Generation Sequencing technology (NGS)
4.1.5 Introduction to Databases - Biological application and Classification
4.2 Biological Databases and retrieval techniques
4.2.1 Nucleotide Databases - Genbank, Unigene
4.2.2 Literature Database - Pubmed, Medline
4.2.2 Protein Sequence Databases - Swissprot, PIR
4.2.3 Protein Structural Databases - PDB, SCOP, CATH
4.2.4 Metabolic pathway database - KEG G, Metacyc
4.2.5 Other databases - OMIM, Taxonomy
08
07
Course Code Title Credits
PSBCH104 Research Methodology, Biostatistics &Soft Skills Development -
1 4
Unit I: Research & Research Design
1.1 Research
1.1.1 Meaning of research, Research Process, Types of research
1.1.2 Formulating research problem
1.1.3 Criteria for good research. Significance of research.
1.2 Research Design
1.2.1 Meaning, features of good research design, types of research
designs. Desc riptive, analytical .
1.2.2 Basic principles of experimental designs. Prospective, retrospective,
prospective & retrospective, observational, experimental, clinical trials,
RCT,Cohort, cross sectional and case controlled studies . Number
of
Lectures
08
07
Unit II: Presentation & Processing of Data
2.1 Scales of measurement
2.2.1 Nominal, Ordinal, Interval, Ratio, Discrete, Continuous
2.2 Descriptive Statistics: Presentation of Data
2.2.1 Diagrammatic Presentation: Graphs and Charts; Tabular presentation
2.2.2 Skewness, Kurtosis, Quantiles, Outliers
2.2.3 Measures of Dispersion: Quartile deviation, Mean deviation,
2.2.4 Standard deviation, Standard error, Variance, Coefficient of variation
Measures of skewness (Karl Pearson, Bowley)
2.3Normal Distribution.
2.3.1 Probability mass/density function, cumulative mass/density function
2.3.2 Properties of Normal distribution
05
10
Unit: III:Analysis of Data and Sampling Techniques
3.1 Correlation and regression analysis
3.1.1 Simple correlation and regression.
3.1.2 Multiple correlation and regression, partial correlation, logistic regression.
3.1.3 Partial correlation analysis.
3.2 : Analysis
3.2.1 Univariate and multivariate analysis. Brief introduction to three main
frameworks: Monte -Carlo analysis, Parametric analysis, Bayesian analysis
3.2.2 Data Analysis: Computing sums of squares, standard error of differences
between means, fitting data to linear model, variances and covariance s,
least square parametric estimation, hypothesis test with regression
3.2.3 ANOVA and partitioning of sum of squares, assumptions, hypothesistests
07
08
with ANOVA, constructing F Ratios, ANOVA Tables, Analysisof
categorical data, two way contingency tables , Chi -square & G -Test
3.3 Power Analysis
3.3.1 Power analysis for contingency tables, t tests, ANOVA, correlation &
regression models. Sample size calculation.
3.4 : Measures of Association
3.4.1 Yule’s coefficient of association
3.4.2 Spearman’s Rank correlation coefficient
3.5 : Sampling
3.5.1 Representative sample, sample bias
3.5.2 Sampling techniques - Simple random, Systematic, Stratified, Cluster ,
Multistage
Unit: IV Soft Skills - I
4.1 : Introduction to Soft Skills
4.1.1: Personality Development – Self Esteem, Positive Thinking, Johari
Window, Physical Fitness
4.1.2 : Emotional Intelligence (EI) & Quotient (EQ) - Meaning, Components of
EI, IQ v/s EQ, Components of EI, Skills to develop EI
4.1.3 : Etiquettes & Manners – Meaning, Professional & Technology etiquettes.
4.1.4 : Communication Skills – Process & Significance of Communication,
Verbal, Non- verbal, formal & informal communication, Barriers,
Techniques to improve LSRW, Intercultural & Digit al Communication .
4.2 : Other Topics
4.2.1 : Leadership & Team Building - Leadership Types/ Styles/ Trails/ Trends,
Types of Teams & Team Building, Group Dynamics.
4.2.2 : Decision Making - Introduction, Steps/ Techniques/ Process of
Decision Making, fundamentals/ Styles/ Major Concepts of Negotiations.
4.2.3 : Stress & Time Management - Sources of &ways to cope with
stress, Planning & scheduling of work/ professional/ rewinding/
refreshing/ hobbies.
4.2.4 : Entrepreneurship & Start -ups – Employment v/s self- employment,
Govt. Schemes & funding Agencies for start – ups.
07
08
Detail Syllabus for
Semester - I Practical
PSBCH P101: Calorimetry, Volumetry, Enzymology, Buffers and Microscopy
1. Estimation of : Φ
a. Proteins by Biuret, Bradford &Folin -Lowry methods
b. Amino acids by Ninhydrin method
c. Glucose by Anthrone&Folin -Wu methods.
d. Percentage Purity of Start from Starch Hydrolystate by Willstatter’s method.
2. Enzymology: Φ
a. Amylase (Km, optimum pH, optimum temperature) from Sweet Potatos
b. Urease (Km) from Jack Beans Meal/ Soya been Seeds
c. Lipase (Km) from Ground Nut Seeds
d. Transaminase (Km) from Germinating Moong Seeds
3. Buffers and Microscopy : §
a. pka values of Ala or Gly by Titration Curve
b. Microscopy: (Permanent Slides may be used)
i. Gram Staining
ii. Spores Staining
iii. Capsule Staining
iv. Acid Fast Staining
PSBCH P102: Biochemical/ Clinical Analysis
1. Estimation of: ( from blood/plasma/serum/urine)
a. Glucose by GOD -POD Method §
b. Tryglycerides §
c. Cholesterol by Zak and Zaltsky Method §
d. Calcium (Ca) by Clark and Collip Method/ Trinder Method Φ
e. Iron (Fe) by Dipyridyl Method Φ
f. Copper (Cu) by Dithiocarbonate Method Φ
g. Phosphorus (P) by Fiske - Subbaraow Method Φ
Φ Experiments for Semester End Practical Examination (30Marks )
§ Experiments for Internal Assessment in Practical (20Marks)
PSBCH P103 : Isolation, Preparation, Extraction and Assays
Extraction, isolation, partial purification (if necessary), calculation of percentage yield and
performing a confirmatory test for the following (for serial nos. 1 to 4)
1. Proteins :
a. Casein from milk Φ
b. Albumins and globulins from egg white §
c. Proteins from ge rminating seeds §
2. Enzymes: Extraction, Partial Purification of the following enzymes and determination of
their Km values
a. GPT from Germinating Moong Seeds Φ
b. Alkaline Phosphatase from Germinating Moong Seeds Φ
3. Estimation of Sodium Benzoate from Jam/ Jelly §
PSBCH P104 : ResearchMethodology and Biostatistics
1. Preparation of Research Proposal for Minor / Major Research Projects to be submitted to
the funding agencies §
2. Review of Research work being carried out at any five National/ International Research
Centers or Institutes §
3. One numerical problem each on Φ
a. Measurement of Central Tendency ( Mean, Median,Mode)
b. Measurement of Dispersion/variability ( Mean Deviation, Standard Deviation,Co
efficient of variation)
Φ Experiments for Semester End Practical Ex amination (30Marks )
§ Experiments for Internal Assessment in Practical (20Marks)
Detail Theory Syllabus
Semester II
Course Code Title Credits
PSBCH201 Advanced Bioorganic Chemistry 4
Unit I:
1.1 Plant Biochemistry
1.1.1 Chlorophylls and accessary pigments
1.1.2 Photosynthesis -Light and Dark Phases, Schemes -I, II & Z,
Cyclic and Non -cyclic Photophosphorylation, C -3 & C -4
Pathways
1.1.3 Biosynthesis of Starch Sugars and Cellulose from Glucose
1.1.4 Photorespiration and photoperiodism
1.1.5 Plant growth hormones - Auxins -Gibberellines Cytokines
Abscisic Acid and Ethylene
1.1.6 Photosynthesis in Microbes, Bacteria, Fungi, Algae and Yeast
1.1.7 Nitrogen Fixation and Sulphur Assimilation in Plants.
Number of
Lectures
15
Unit II:
2.1 Endocrinology
2.1.1 Organization of Mammalian Endocrine System, Classification
of Hormones . Biosynthesis, Storage, Secretion, Transport and
Metabolic effects (including hypo and hyper conditions) of
Hormones of Pituitary, Hypothalamus, Thyroid, Parathyroid,
Pancreas, Adrenal Medulla, Adrenal Cortex, Gonads, Kidneys
and G I Tract.
2.1.2 Mechanisms of Hormone action, Role of Secondary
Messengers -cAMP, cGMP, Ca and Calmodulin
Cell membrane and intracellular receptors for hormones
Regulatory pathways (positive, negative, feedback loops)
2.1.3 Endocrine regulation of growth, stress, hung er, digestion,
obesity, renal function, cardiovascular system (angiotensin,
BNP, endothelin 1 (ET -1)Endocrinology of fertility (Changes
in menstruation, pregnancy & menopause). Medical uses of
steroid hormones (contraception, HRT, hydrocortisone,
anabolic steroids). Erythropoietin, Adipocytokines, O rexins
05
05
05
Unit: III
3.2 Biochemistry of Tissues
3.1.1 Muscles - Structure and composition of muscle fibres,
mechanism of muscle contraction and relaxation, energy source
for muscular work, muscular dystrophies
3.1.2 Bones - Composition, formulation, Structure and functions,
factors affecting bone metabolism, bone remodelling,
osteoporosis, osteomalaciaNerves - Structure of Neuron,
chemistry of nerve tissue, mechanism of nerve impulse
transmission, synapse an d synaptic transmission, Synthesis and
actions of neurotransmitters (GABA, Acetylcholine,
Dopamine), disorders related to defects in neurotransmission –
(Parkinson’s disease, stroke, Alzheimer’s
disease) Biochemistry of memory mechanisms, Blood Brain
Barrier, Sensory Receptors of Taste, Vision, Odour, Hearing,
Touch
3.1.3 Connective Tissue - Biosynthesis, composition, structure and
metabolism of Collagen and its Disorders -Ehler’s Syndrome
(Type I to VII), OsteogenesisImperfecta (Type I to IV ), Paget’s
disease
15
Unit: IV
4.1 Bioluminescence
4.1.1 History, Source of Bioluminescence material, examples of
bioluminescence organism
4.1.2 Mechanism of Bio -luminescence in specific organisms,
Evolution and Bioluminescence.
4.1.3 Use and applications of bioluminescence
4.2 Unusual Bio-molecules
4.2.1 Prions, Fullerenes, Small Nuclear Riboproteins (SNURPNs),
Lectins, Antifreeze proteins, Stress Proteins, Chaperons,
Ionophores (Crown ethers, Cryptans)
4.2.2 Biomimetic Chemistry - Mimicking of Ion Channels, Enzyme
receptor carriers, antibodies, Vesicles and Sensors,
Enzyme mimicking -Cram’s Protease Model, Rebok’s allosteric Model
and Flavinophores for NAD
4.2.3 Host -guest Chemistry -Cyclophanes, C alixanes, Cyclodext rins,
Cyclop eptides.
4.3 Natural Bioactive Compounds
4.3.1 Industrially & pharmacologically important primary &
secondary metabolites from living cells
4.3.2 Occurrence/ Source, Chemistry, Isolation, Biosynthesis
(Schematic) mode of action and physiological significance/
importance/ application of Antibiotics, Toxins & Glycolipids
05
05
05
from bacteria, enzymes, isoprenyl glycerol ethers from
Archaea, Antib iotics, anticancer agents & ACE Inhibitors from
fungi, Venoms (snakes & scorpions), ω -conotoxin (marine
snail), ecteinascidin (tunicate), discodermolide (sponge),
aleutherobin (coral) and biostalins (bryozoan) from a nimals,
Phytochemicals (phenols, polyphenols, tannins, terpenes &
alkaloids) from plants.
Course Code Title Credits
PSBCH202 Advanced Instrumentation and Analytical Techniques 4
Unit I:
1.1 Centrifugation
1.1.1 Basic principles of sedimentation, relation between g and rpm
1.1.2 Principles, Instrumentation, Working and Applications of
Preparative and Analytical Ultracentrifugation, Isopycnic
Centrifugation, Rate Zonal Centrifugation
Number of
Lectures
15
Unit II:
2.1 Chromatography
2.1.1 Basic Principles, Instrumentation, working and applications of
partition chromatography (Paper), Absorption
Chromatography (TLC, HPTLC, Column), Affinity
Chromatography, Ion Exchange Chromatography, Permeation
Chromatography, Gas-Liquid Chromatography (GLC), High
Pressure Liquid Chromatography (HPLC)
15
Unit: III
3.1 Electrophoresis
3.1.1 Basic principles, factors affecting electrophoresis, support
media used
3.1.2 General principles, instrumentation, working and applications
of electrophoretic techniques -zone, Disc, Capillary, 2 -D,
Pulsed Field Gel, Diagonal, Isoelectric Focussing, immune -
electrophoresis
3.1.3 Gel Documentation System
3.2 Sequencing Techniques
3.2.1 Basic Principles and Instrumentation, working and
applications of -
Purification of Proteins/ Enzymes
08
07
Protein Sequencing Techniques
DNA Sequencing Techniques
RNA Sequencing Techniques
Blotting Techniques
Unit: IV
4.1 Special Instrumentation Methods and Analysis
4.1.1 Basic Principles, Instrumentation, working and applications of
experimental techniques in Flow Cytometry Electroporation,
Autoclave, Inspissator
4.1.2 Basic Principles, Instrumentation, working and application of
instrumental methods of analysis in environmental methods of
analysis in environmental biochemistry - Conducto metry,
Potentiometry , Selective Ion Meters, High Frequency
Titrations, Polarography, Anode Stripping Voltammetry,
Neutron Activation Analysis, Inductively Couples Plasma
Emission Spectrometry
15
Course Code Title Credits
PSBCH 203 Industrial Biochemistry and Bioinformatics - 2 4
Unit I: Carbohydrates, lipids and proteins of Industrial Importance
1.1 Carbohydrates of industrial importance
1.1.1 Manufacturing and refining of cane sugar , starch, pectin &
cellulose
1.1.2. Manufacturing of polysaccharides . Plant polysaccharide
(Gum Arabic) , microbial polysaccharides , modified
carbohydrates – modified starches, modified celluloses.
1.2. Lipids of industrial importance
1.2.1 Extraction and refining of vegetable oils and animal fats in
general.
1.2.3 Extraction and applications of chlorophyll, carotene, lycopene
Turmeric, and essential oils.
1.3 Proteins of industrial importance
1.3.1Isolation and purification of Proteins & Enzymes – Source
ident ification, isolation, recovery, concentration.
1.3.2 Partial/total purification by salting in, salting out, precipitation,
ion exchange, dialysis, ultra -filtration, column chromatography
(Gel filtration, Affinity, HPLC)
1.4 Enzyme Immobilization
1.4.1 Methods of immobilization Number of
Lectures
04
03
03
1.4.2 Applications in industry and medicine
1.5 Production of vaccines, hormones and industrial proteins
1.5.1 Vaccines & Anti – toxoid Technology for measles, poliomyelitis,
typhoid, Hepatitis B, AIDS, anti –tetanus.
1.5.2 Hormones – conventional & engineered Insulin, Erythropoietin,
Growth hormones
1.5.3. Non – catalytic industrial proteins – casein, whey proteins,
Egg proteins, wheat germ proteins.
Unit II: Environmental Biotechnology
3.1 Air Pollution
3.1.1 Air pollution – classification & effects of air pollutants on human
health, Gases containing the oxides of carbon, sulphur and
nitrogen, ozone and CFC. Measures to control air pollution and
suspended particulate matters in air.
3.1.2 Green house effect & Global warming – sources, consequences &
remedial measures.
3.2 Water Pollution
3.2.1 Sources and effects of water pollutants on human health, quality
standards for drinking water, waste water treatment and recycling.
3.2.2 Concept and significance of BOD, COD and dissolved oxygen.
3.3 Noise Pollution
3.3.1 Sources, mea surement, health hazards, prevention & control of
noise pollution.
3.4 Toxins in environment
3.4.1 Chemical toxicology – Biochemical effects of heavy metals (Pb,
As, Hg, Cd), pesticides, insecticides, herbicides, weedicides,
larvicides, polyaromatic hydrocarbons, dy es, monomeric and
polymeric organics.
3.4.2 Emerging eco -friendly alternatives for chemical industry –Green
chemistry and Green Technology.
04
04
02
05
Unit : III Nanotechnology and other topics
4.1 Nano biotechnology
4.1.1 Definition and methods of preparation of nano -bioparticles.
4.1.2 Applications in drug designing, drug delivery & protein
engineering.
4.2 Other Topics
4.2.1 Biosensors – Construction, uses in industrial and environmental
processes and medical applications.
4.2.2 Principles, techniques and applicati ons of – Liposome formation,
07
08
natural & synthetic biofilms.
4.2.3 Clinical diagnostics – Diagnostic Kits and their applications.
4.2.4 Concept and significance of Bio safety, Bio Hazards and Bio ethics.
4.2.5 Concept of QC, QA GMP, GLP in labs & production processes.
Lab/pro cess validation & Accreditation.
4.2.6 Maintenance & Management of Lab /Experimental animals and
Animal House CPCEA guidelines.
Unit: IV - Bioinformatics II
4.1 Genomic and Protein Sequence Analysis
4.1.1 Pair wise sequence alignment, gaps, gap-penalties, scoring
matrices - PAM250, BLOSUM62, Local and global sequence alignment
4.1.2 Nucleotide and Protein sequence analysis using BLAST and variants
4.1.3 Introduction to multiple sequence alignment - Progressive
algorithms - Clustal programs.
4.2 In-silico Protein structure prediction
4.2.1 Introduction to protein structure
4.2.2 Protein -protein interaction
4.2.3 Computational methods in protein Secondary structure prediction
4.2.4 Computational methods in protein Tertiary structure prediction -
Homology modelling
08
07
Course Code Title Credits
PSBCH204 Research Methodology, Biostatistics &Soft Skills
Development. 4
Unit I: Report Writing & Presentation
1.1 Report Writing
1.1.1 Significance of report writing, different steps in report writing,
typesof report .
1.1.2 Mechanics and precautions of writing research reports for
scientific journals, popular magazines, seminars/symposia/
conferences/workshops
1.1.3. Layout of research paper, Layout for poster.
1.2 Presentation
1.2.1 Presentation – Oral & Written . Use of digital media .
1.2.2 Presentations in classrooms, scientific meets & public audience. Number of
Lectures
08
07
1.2.3 Defence of research thesis.
Unit II: Estimation and Testing of Hypothesis.
2.1.Properties and use of Normal Tables
2.2 Estimation and Testing of Population Parameters
2.2.1 Estimator of population proportion and mean and their sampling
distribution
2.2.2 Testing of Hypothesis – Type I and Type II errors, Level of
Significance. Confidence Int erval. Z - test for testing population
mean(s) and proportion(s). t - test for testing population mean
Paired & Unpaired t-test for testing population means
2.3. Measure s of outcome of clinical interventional studies :
2.3.1 Relative Risk(RR), Relative Risk Reduction(RRR) and
Odds Ratio(OR)
07
08
Unit III:Non-parametric tests, Diagnostic Tests & Vital Statistics.
3.1 Non-parametric tests
3.1.1 Importance of non -parametric tests.
3.1.2 Chi square test of goodness of fit.
3.1.3 Chi square t est for independence of attributes & yate’s correction.
3.2 Diagnostic tests
3.2.1 Importance of diagnostic tests
3.2.2 Sensitivity, specificity, positive predictive value, negative
predictive va lue, accuracy, probability and odds ratio , likelihood
ratio(LR ), LR of positive test, LR of negative test.
Receiver operating characteristics (ROC) curves
3.3 Demography & Vital Statistics
3.3.1 Collection of demographic data, vitalstatistics at state & National
levels, reports of special demographic surveys.
3.3.2 Measures of vital statistics: Rate of mortality, fertility,
reproduction, morbidity, comprehensive indicators, indices of
health population growth rates and density of population.
05
04
06
Unit: IV Soft Skills Development – 2
4.1 : Professional Skills
4.1.1 : Creativity at Workplace – Types of Workplace, Creativity/
Motivation/ Innovativeness/ Initiative at workplace.
4.1.2 : Ethical Values – Ethics/ Values/ Morals, Nurturing work ethics,
Gender , neutrality , Human Rights
4.1.3 : Capacity Building – Learn, Unlearn & Relearn, Skills for capacity
building, Zones & Ideas for Learning, Strategies for capacity
07
building.
4.2 Academic Skills
4.2.1 : Employment Communication – CV & Resume Building,
Scan able CV, Formats of CV/ Resume/ Job Application/ Covering
Letter, Professional presentations.
4.2.2: Job Interviews – Background information, Types & preparatory
steps for interviews, developing interview skills, mock interviews,
FAQs in interviews.
4.2.3 : Group Discussion – Ambience & Seating arrangements for GD,
Importance & significance of GD, GD/ Panel Discussion/ Debate,
Types of GD ( Topics – based & Case - based), Analysis of personal
traits in GD.
08
Detail Syllabus for
Semester - II Practical
PSBCH P201: Chromatography and Electrophoresis Techniques
1. Extraction of Plant Pigments from Spinach Leaves and their separation by Column
Chromatography Φ
2. Assay of Hormone
3. Chromatography: Φ
a. Ascending and Circular paper for Amino Acids and Sugars
b. TLC of Oils
4. Electrophoresis: Φ
a. Serum Proteins Electrophoresis (Agar/Agarose)
b. Haemoglobin Electrophoresis (Normal/Abnormal)
5. Separation of Glucose and Starch (Gel filtration) §
6. Separation of Starch and Casein (Gel filtration) §
PSBCH P202 :Clinical Estimations
1. Estimation of: from blood/plasma/serum/urine)
a. Creatinine by Jaffe’s method Φ
b. Blood Urea Nitrogen (BUN)by DiacetylMonoxime Method Φ
c. Uric Acid by Caraway method Φ
d. Haemoglobin by Drabkin Method §
e. Differential Proteins (A/G Ratio) by Reinhart Biuret Method §
PSBCH P203 : Extraction, Isolation, Partial Purification Techniques
Extraction, isolation, partial purific ation (if necessary), calculation of percentage yield and
performing a confirmatory test for the following:
1. Carbohydrates:
a. Cellulose from Grass §
b. Glycogen from Liver Φ
c. Starch from Potato Φ
d. Pectin from apples/bananas/oranges §
2. Lipids:
a. Estimation of Lecithin and cholesterol from egg yolk Φ
b. Estimation of Essential oils from orange peels Φ
3. Pigments (Separation of the following pigments on TLC slides): Φ
a. Curcumin from Turmetic
b. Carotenes from carrots
c. Chlorophylls from spinach
4. Estimation of §
a. Total Alk alinity of Water Effluent
b. COD of Waste Water
c. Total Hardness of Well Water
d. Chlorides from Water Sample by Schales&Schales Method
PSBCH P204 : Biostatistics and Bio -informatics
1. One numerical problem each on Φ
a. Z-Test
b. T-Test
c. Chi-Squares Test
d. Simple Regression
e. Multiple Regression
2. Bioinformatics - §
a. Searches on Medline, PubMed and BioMed Central
b. Use of Clustal X/W for alignment of protein and nucleic acid sequences
c. Use of TAXON to classify Microbes and Viruses
d. Methods of searching databases like BLAST and FASTA
3. Student will be required to:
a. § Access at least five scientific websites to collectrelevant information with
respect to the topics from the syllabus assigned to him or her by the teacher. A
one [page summ ary per website visited (i.e. a total of five pages) should be
entered in the journal as a part of practical IV. Teacher should encourage that
different topics from the theory syllabus are given to student and student would
access as far as possible different web sites form information collection.
b. § Select any two researchpapers from any leading nation and international
scientific journals (not older than two years) and present these papers in his or her
biochemistry department as if it his/her own research work. A one page summary
per research work. A one p age summary per research paper presented (i.e. a total
of two pages) should be entered in Journal as a part of practical IV. (Teacher may
help students in selecting such research papers from the scientific journals
available at their respective colleges or at other institutions/libraries). A
compilation of research papers entitled “Papers in Bio -chemistry” edited by John
Herriott, Gary Jacobson, Julius Marmur and William Parson published by
Eddison -Wesley Publications Co. Menlo Park, California, USA may be referred
to for classical original papers in biochemistry representing milestone discoveries
in bio -chemistry such as Krebs Cycles, Structure of Myoglobin and Haemoglobin,
etc.
A computer terminal with an internet connection will have to be made available in the
department of biochemi stry for conducting Practical 204 .
Φ Experiments for Semester End Practical Examination (30Marks )
§ Experiments for Internal Assessment in Practical (20Marks)
Suggested Readings for Paper 101,201 and Practical 101,201.
Ref:
1. Bioenergetics (Fourth Edition), David G. Nicholls and Stuart Ferguson,
Academic Press, 2013.
2. Fundamentals of Enzymology, Price.NC. And Stevens. L., Oxford University Press
3. Enzymes - Biochemistry, Biotechnology, Clinical chemistry - Palmer, T.,
Affiliated East -West press
4. Fundamentals of Enzyme Kinetics, Segel I H; Wiley Interscience,
5. Biochemical calculations, 2nd Edition by Irwin H. Segel. John Wiley & Sons,
6. Lehninger Principles of Biochemistry, David L. Nelson, Michael M. Cox
Publisher: W. H. Freeman
7. Enzymology by Dixon and Webb
8. Enzymes by Palmer
9. Biochemistry. Jeremy M. Berg, John L. Tymoczco, and Lubert Stryer: W.H. Freeman
10. Biochemistry. (4th Ed.). Donald Voet, Judith G. Voet – Publisher John Wiley & Sons.
11. Membranes and their cellular functions - IB Filnean, R. Coleman and R.H. Mitchell,
1984, Blackwell Scientific Publishers, Oxford, 3rd ed.
12. The cell by Geoffrey M. Cooper
13. Cell biology Deroberties
14. Illustrated Biochemistry Harpers 30th ed
15. Molecular biology of cell by Alberts 5th ed
16.Cell and molecular biology by Lippincott's illustrated Reviews
17. Williams Textbook of Endocrinology –Larsen, R.P. Korenberg, H. N. Melmed , S.
and Polensky, K. S. Saunders .
18. Human Physiology –Chatterjee.C.C, Medical Allied Agency
19. Principles of Biochemistry: Mammalian Biochemistry: Smith EL, Hill RL,
White A, McGraw Hill
20. The metabolic basis of Inherited diseases (Vol I & II) Scriver CR..Valle D,
Pub McGraw Hill
21. Vertebrate endocri nology. Norris DO (1985) 2nd Edition.
22. Endocrine Physiology - Martin C.R., (1985). Oxford University Press) N.Y.
23. Biochemistry. Zubay (1983) Addison, Wesley publ. Co.
24. Biochemical Endocrinology. E. Frieden (1983)
25. Chemistry of Natural products by SV Bhat, BA Nagasampagi & M
Sivakumar, Berlin Springer (2005) (ISBN 3-540-40669 -7)
26. Handbook of Pharmaceutical Natural Products by G Brahamachari,
Wiley -VCH (2010) (ISBN 978 -3-52732148 -3)
27. The secondary Metabolites Natural Products by JR Hansen, Royal
Society of Chemistry (2003) (ISBN 0-85404 -490-6)
28. Natural Products from Plants by PB Kaufman, CR press (1999)
(ISBN 0 -8493 -3134 -X)
29. Medicinal Chemistry of Bioactive Natural Products by X T Liang,
WS Fang (Eds), Wiley Interscience (2006) (ISBN 0471 -73933 -2)
Suggested Readings for Paper 102,202 and Practical 102,202.
1. Van Holde KE – Principles of Physical Biochemistry, Prentice Hall, 1998
2. Wilson K & Walker J – Principles and Techniques of practical Biochemisty. Cambridge Low
Price Edition
3. Frelfelder D - Physical Biochemistry
4. Skoog Douglas A – Principles of Instrumental Analysis Harcourt Brace publishers, London
5. Harvey David – Modern Analytical Chemist ry, International edition, McGraw, Hlll, Boston
6. Srivascava VK and Kishor K – Introduction to chromatography: Theory & Practice, S Chand
& Co, New Delhi
7. Holme David J – Problem solving in analytical biochemistry, H & Longman Sc. And Tech,
Essex
8. Brave Robert D – Introduction to Instrumental Analysis, McGraw Hlll Book Co, New York
9. Ninfa Alexander J and Ballou David P – Fundamental Laboratory Approaches for
Biochemistry and Biotechnology, Fitzgerald Science Press, Bethesda
10. Upadhyaya et al – Biophysical Chemistr y, Himalaya Publishing Home, New Delhi
11. Rodney Boyer Experimental Biochemistry Pearson Publ. Sawheny and Singh
12. Practical Biochemistry by David Plummer
13. Methods in Enzymology – Kaplan
Suggested Readings for Paper 102,202 and Practical 102,202 :
1. Greenberg David M – Metabolic Pathways. Vols . 2 and 3, 3rd editions. Academic Press, New
York
2. Henry Richard et al – Clinical Chemistry, Principles and Techniques, 2nd edition, Harper and
Row, New York
3. Kamal SH – Clinical Biochemistry for Medical Technologies, Church ill Livingston, London
4. Todd et al – Clinical Diagnosis and Management, 17th edition, WB Saunders, Philadelphia
5. Stokes Joan et al – Clinical Microbiology, Edward Arnold, London
6. Gill CV – Short cases in clinical biochemistry, Churchill Livingston, Edinburgh, 1984
7. RaoRanganathan – Text book of biochemistry 3rd edition, Prentice Hall, New Delhi
8. Rodrigues Fred K Carbohydrate chemistry with clinical correlations, New Age International,
New Delhi
9. BayensDominiezak – Medical biochemistry, Mosby Publishers, Harcourt, 1999
10. John Bernard Henry , Clinical Diagnosis and Management by Laboratory Methods, Saunders
publications, 20th edition
11. Feitz – Clinical Chemistry
12. Nelson DI, Cox MM – Lehninger Principles of Biochemistry
13. Murrary Robert – Harper’s biochemistry, 24th edition, Prentice Hall International UK LTD,
1990
14. Satyanarayanan – Biochemistry
15. Vasudevan Text Book of Medical Biochemistry
16. Voet&Voet – Biochemistry, 2nd edition
17. Chatterjee and RanaShinde Medical - Biochemistry
Suggested Reading for Paper 103,203 and P ractical 103,203 :
1. Industrial Microbiology – AH Patel, McMillan India Ltd, 1st Edition
2. Food Microbiology – Frazier &Westhoff, Tata McGraw Hill Publishers, New Delhi
3. Total synthesis of natural products, Vol I -John Apsinon
4. Chemical Process Industries – Norris Shreeve& Joseph Brink
5. Roger’s Industrial Chemistry Vol I & II – Edited by CC Furnas
6. Merck Index, 10th Edition
7. Encyclopedia of chemical technology, 3rd Edition
8. Chemistry of Natural Products – Agarwal& Sharma
9. Industry chemistry of Fats and Waxes – JP Hilditch
10. Essential Oils, Vol I – Ernst Guenther
11. Natural and Synthetic colouring matter and related fields – JS Gore, Joshi
12. Encyclopedia Britannica, Vol IV & V
13. Principles of Environmental Chemistry – Kothandaram&Sw aminathan, BI Publishers,
Chennai
14. Environmental Chemistry – AK De, New Age International Publishers, 4th Edition
15. Molecular Biology and Biotechnology – Edited by JM Walker & EB Gingdd, Panima
Educational Book Agency, New Delhi, 2nd Edition
16. GMP for pharmaceu ticals, A plan for TQC – SH Wiling & JR Stoker, Marul Dekker Inc.,
New York, 4th Edition, 1997
17. Total Quality Assurance for the Food Industries – WA Gould & RW Gould. CTI
Publications Inc., USA 1988
18. Current Good Manufacturing Practices for Food Plan Sanitat ion – WA Gould, CTI
Publications Inc. USA 1980
19. Fundamental Concepts of Environmental Chemistry – Sodhi, Narosa Publishing House, 2002
20. Introduction to plant Biotechnology – HS Chawla, oxford & IBH Publishing Co, New Delhi,
2nd Edition.
21. Nanotechnology, A Gen etic Introduction to the next big idea – Mark Ratner & Daniel Ratner,
Pearson Education
22. Animal Biotechnology – Edited by AK Srivastava , oxford & IBH publishing Co, New Delhi,
2005
23. Proteins, Biochemistry & Biotechnology – Gary Walsh, John Wiley & Sons, 2002
24. Biotechnology, An Introduction – Susan R Barnum, Vikas Publishing House, International
Student Edition
25. Enzymes, Biochemistry, Biotechnology, Clinical Biochemistry – Trevor Palmer, First East -
West Press Ed. 2004
Suggested Readings for paper 103,203 and P ractical 103,203 :
Suggested Readings for paper 104,204 and Practical 104,204 :
1. Lemeshow S, Homer DW, Klar J and Lwanga SK, (1996) Adequacy o sample size in
health studies. John Wiley and Sons, Chichester .
2. Machin, D., Campbell MJ, Fayers P, Pinol A., (1998) Statistical Tables for the design
of Clinical Studies, Second Edition, Blackwell Oxford.
3. Quinn & Keough, Experimental Design and Data Analysis for Biologists, Cambridge
University Press. http://www.lacbiosafety.org/wp -
4. Statistical methods for resear ch workers – RA Fisher, 14th edition, Oliver Boyd publication
5. Statistical methods in research and production – Davelr OL & Goldsmith PL, Longman
6. Methods in biostatistics for medical students and research workers – BK Mahajan, Jaypee
Brothers, New Delhi
7. Research methodology, Methods and techniques – CR Kothari Willey Eastern Ltd, Mumbai
8. Research methods – Ram Ahuja, Rawat Publications, New Delhi
9. Genetics and Biostatistics – MeyyanPillai, Saras Publication, Kanyakumari
10. Papers in Biochemistry – Jon Herriott Gary Jacobson, Julius Marmur and William parson,
Addison -wesley publication Co, California
11. Experimental Biochemistry, Theory and exercises in fundamental methods – Robert Switzer
and Liam Garrity, 3rd edition, WH Freeman & Co. NY
12. Computational methods in M olecular Biology – SL Slazberg (ed), Elsevier Science
13. The Molecular Biology Database Collection, An updated compilation of biological database
resources in Nucleic Acid Research 2001, Academic Press
14. Using cluster for multiple sequence alignment – Higgins D G, Methods in Enzymology, 256,
Academic Press
15. Modelling cellular behaviours – Endy& Brent, Nature, 409:391 -395, 2001
16. Cracking the genome – Kavin Davis, Scientific American Publ.
17. Statistical methods in biological array – Davids J Finney, 3rd edition charl es Griffin & co,
London
18. Research Methodology – SM Israney, universal Publishing Corporation
19. Bioinformatics, Methods and protocols – edited by Stephen Messens and Stephen Krawetz,
humana press, Totowa, New Jersey
20. Bioinformatics, A practical guide to analysi s of genes and proteins – Andreas P Baxevanis
and BF Francis Quellette, Wiley Interscience, 1998
21. Statistics for Biology – Bishop ON, 1983, Longman
22. A Textbook of Biostatistics by A Annadurai, New Age Publication
23. Bioinformatics - Methods and Applications by S C Rastogi, N. Mendiratta& P. Rastogi, PHI
Learning Pvt. Ltd.
Scheme of University Examination for M.Sc. Degree (by papers) in Theory &
Practical in Biochemistry to be brought in force from 2012 -2013 & progressively
thereafter as Credit Based Semester And Grading System .
A Distribution of Credits
Credits for Theory Credits for Practicals
Paper Credits per Semester Practical Credit per Semester
Each 4 Each 2
Total Number of
Semesters Number of Theory
Papers per Semester Total Number of
Theory Papers Total Number of
Credits
4 4 16 16 X 4 = 64 (a)
Total Number of
Semesters Number of
Practicals per
Semester Total Number of
Practicals Total Number of
Credits
4 4 16 16 X 2 = 32 (b)
Total Number of credits for MSc degree by papers in Biochemistry (a) + (b) = 96
B Distribution of Marks
Theory
Paper Theory 100 Marks per paper Practicals
(50 Marks/Practical) Semester End Theory
Exam. (60) Internal Assessment
(40)
No.
of
Units Marks
per
Unit Total
Marks Class Test
(1 X 20
Marks) Assessment
(2 X 10
Marks) Practical Semester
End
Practical Internal
Assessment
(Marks)
Exam.
(Marks)
Each 04 15 60 20 20 Each 30 20
Year Semester Total Theory
Marks
(a) Total Practical
Marks
(b) Grand Total
(a) + (b)
M. Sc. Part I I 4 Papers X 100 =
400 Marks 4 Practicals X 50
= 200 Marks 600 Marks
II 4 Papers X 100 =
400 Marks 4 Practicals X 50
= 200 Marks 600 Marks
M. Sc. Part II I 4 Papers X 100 =
400 Marks 4 Practicals X 50
= 200 Marks 600 Marks
II 4 Papers X 100 =
400 Marks 4 Practicals X 50
= 200 Marks 600 Marks
2400 Marks
Use of a simple calculator shall be permitted for solving numerical and statistical problem at
theory and practical examination.
1. Duration of practical examination
a. Semester -end practical examination
i. One Day practical examination with two sessions of three hours thirty minutes each, i.e.
Session I - 9am to 12:30 pm and Session II - 1:30 pm to 4:30 pm. With lunch break from
12:30pm to 1:30pm
ii. Examination of five experiments (not more than two experiments from each practical)
for 20 marks each shall be conducted over two sessions, including viva voce
examination ..
iii. Only those experiments indicated by symbol Φin practical syllabus shall be asked in
semester -end practical examination .
iv. Only those expe riments indicated by symbol §in practical syllabus shall be asked in
the internal practical examination.
8. Each candidate is required to submit a certified journal for each of the semesters at the time
of semester -end practical examination.