B Voc MLT Revised Syllabus 2020 21_1 Syllabus Mumbai University

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Copy to : -
1. The Deputy Registrar, Academic Authorities Meetings and Services
(AAMS),
2. The Deputy Registrar, College Affiliations & Development
Department (CAD),
3. The Deputy Registrar, (Admissions, Enrolment, Eligibility and
Migration Department (AEM),
4. The Deputy Registrar, Research Administration & Promotion Cell
(RAPC),
5. The Deputy Registrar, Executive Authorities Section (EA),
6. The Deputy Registrar, PRO, Fort, (Publi cation Section),
7. The Deputy Registrar, (Special Cell),
8. The Deputy Registrar, Fort/ Vidyanagari Administration Department
(FAD) (VAD), Record Section,
9. The Director, Institute of Distance and Open Learni ng (IDOL Admin),
Vidyanagari,
They are requested to treat this as action taken report on the concerned
resolution adopted by the Academic Council referred to in the above circular
and that on separate Action Taken Report will be sent in this connection.

1. P.A to Hon’ble Vice -Chancellor,
2. P.A Pro -Vice-Chancellor,
3. P.A to Registrar,
4. All Deans of all Faculties,
5. P.A to Finance & Account Officers, (F.& A.O),
6. P.A to Director, Board of Examinations and Evaluation,
7. P.A to Director, Innovation, Incubation and Linkages,
8. P.A to Director, Board of Lifelong Learning and Extension (BLLE),
9. The Director, Dept. of Information and Communication Technology
(DICT) (CCF & UCC), Vidyanagari,
10. The Director of Board of Student Development,
11. The Director, Dep artment of Students Walfare (DSD),
12. All Deputy Registrar, Examination House,
13. The Deputy Registrars, Finance & Accounts Section,
14. The Assistant Registrar, Administrative sub -Campus Thane,
15. The Assistant Registrar, School of Engg. & Applied Sciences, Kalyan ,
16. The Assistant Registrar, Ratnagiri sub -centre, Ratnagiri,
17. The Assistant Registrar, Constituent Colleges Unit,
18. BUCTU,
19. The Receptionist,
20. The Telephone Operator,
21. The Secretary MUASA

for information.

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AC - ___________
Item No. ____________





















UNIVERSITY OF MUMBAI


Syllabus
for the
Program : Bachelor of Vocation (Medical
Laboratory Technology)
Sem. - I, II, III , IV, V & VI
(Choice Based and Credit System with effect from
the academic year 2020 -21)

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AC - ___________
Item No. ____________

UNIVERSITY OF MUMBAI

Sr.
No. Heading Particulars
1 Title of the Course Bachelor of Vocation (Medical Laboratory Technology)
(B.Voc. M.L.T.)
2 Eligibility for Admission 12th Pass (Science / MCVC (MLT)
12thPass in Arts / Commerce with D.M.L.T 2 or 3 years of
MSBTE or any UGC Recognized University or NSDC)
3 Passing Marks 40%
4 Ordinances / Regulations
( if any) --
5 No. of Years / Semesters 03 years (0 6 semesters)
6 Level Certificate / Diploma / U.G. √/ P.G.
( Strike out which is not applicable)
7 Pattern Yearly / Semester √
( Strike out which is not applicable)
8 Status √Revised / New
( Strike out which is not applicable)
9 To be implemented from Academic Year From Academic Year
2020- 21
Date: Signature:
Name Dr. Anuradha Majumdar
Dean, Science and Technology

Chairman/Principal of BoS

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PREAMBLE
Bachelor of Vocation (Medical Laboratory Technology) (B.Voc.M.L.T.) (Choice based
Credit System) Semester - I, II, III , IV, V & VI Revised Syllabus to be sanctioned &
implemented from Academic Year 20 20-21.

As per UGC Guidelines, the result of Job Roles assessment done by NSDC and
Health Sector Skill Council has to be included in the marksheet of B.Voc.(M.L.T.) at
semester I, IV & VI for 18 credit points.

Therefore, the modification in the credit points in existing syllabus and addition of
Skill Component Result of Heathcare Sector Skill Council has been proposed at Semester I,IV & VI.

PROGRAM OUTCOME
1. Knowledge, skills, and values that prepare students for future careers in our
interconnected society, whether in advanced study
2. This programme will also give students an improved sense of self -confidence and self -
efficacy and an awareness of their responsibilities as professionals in their field
3. Learners will comprehend the foundations, process, and practices, and demonstrate
proficiency in Laboratory Technology.
4. Understand the role of Healthcare Professionals .
5. Apply knowledge and perform tests of Clinical Biochemistry, Human An atomy and
Medical Parasitology.
6. Apply knowledge and perform tests in Clinical Immunology, Clinical Pathology &
Haematology
7. Understand the significance of Community Medicine.
8. Understand Risks and apply Preventive Measures and perform diagnosis of epidemics.
9. Gain knowledge to work as a skilled Technician in Blood Banks.
10. Gain knowledge and assist in Blood Transfusion Process.
Dr. Anuradha Majumdar (Dean, Science and Technology)
Prof. Shivram Garje (Associate Dean, Science)
Name of Chairperson (BoS)
Member(BoS)
Member (BOS)
Member(BOS)

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Vidya Vikas Education Society’s
Vikas College of Arts, Science & Commerce
Bachelor of Vocation (Medical Laboratory Technology)
1. General Guidelines
The Credits are defined in terms of the learner’s hours which are divided into two part su
ch as Actual and Notional. The value of a particular course can be measured in number of Credit
Points. The value of One (01) Credit is equal to 15 Hours of learners load.
The scheme of Examination shall be divided into two parts i.e. Internal Assessmen
t includes Assignments, Seminars, Case Studies and Unit Tests which will be of 25 marks and th
e Semester End Examinations which will be of 75 marks.The semester wise Credit Points will be
varied from program to program but the value of Credits for Under Graduate Programmes shall
be of 180 Credits.
Out of 30 credits per semester, the general Education component shall have 40 % of the
total credits (12 credit points per Semester) and balance 60 % credits (18 credit points per
semester) will be of skill component.
2. Credit Based Evaluation System
Scheme of Examination
The assessment for the general education component should be done by the university as
per their prevailing standards and procedures. The universit y has to necessarily establish a
credit based assessment and evaluation system for the B.Voc. Programme. The assessment of skill based components shall be done by the Healthcare Sector Skill Council ( HSSC) in
Semester I, IV and VI as per their prevailing s tandard and procedure, as per UGC
guidelines of B.Voc.
For all 6 semesters, the performance of the learners shall be evaluated into two components. The
first component shall carry 25% marks which will be an internal assessment while the second co
mponent shall carry 75% marks at semester end examination.
The allocation of ma
rks for the Internal Assessment
25% and Semester End Examinations 75% are as shown below:
Examination Pattern (Semester)
A) Internal Assessment (25%) = 25 Marks
• One class test held in the each semester 20 Marks
• Active participation in routine class instructional deliveries
(and in practical work, tutorial, field work etc as the case may be ) 05 Marks

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B) Theory External Examination (75%)= 75 Marks
i) Duration : These examinations shall be of 21/2 Hours duration for each paper.
ii) Theory Question Paper Pattern :
• There shall be five questions each of 15 marks. On each unit there will be one
question and the fifth one will be based on entire syllabus.
• All questions s hall be compulsory with internal choice within the questions.
Each question will be of 20 to 23 marks with options)
• Question may be subdivided into sub questions a, b, c…. and the allocation of
marks depend on the weightage of the topic.

C) Practical Ex ternal Examination = 50 Marks for each paper
D ) Skill Assessment Examination : -
According to UGC Guidelines for B.Voc. scheme, Skill component Assessment will
be done by Sector Skill Council(SSC) under National Skill Development Corporation
(NSDC). The SSC will map the curriculum as per Job Role / Qualification Pack(QP) and
National O ccupational Standards (NOS) with University syllabus. The separate assessment
examination (Theory & Practical) will be conducted by SSC through their authorised Assessment Agencies as per norms and assessment criteria of SSC – NSDC. This skill
Examination result will be provided by SSC to the respective B.Voc. College.The result of
skill assessment examination should be included in the student marksheet as per assessment criteria of SSC. The passing standards will be as per SSC – NSDC rules.
The assessment of Part ‘A’ i.e. Internal Assessment and Part ‘B’ i.e.Semester End Exami
nation as mentioned above for the Semesters I to IV shall be processed by the Colleges ‐‘Instituti
ons of their learners’ and issue the grade cards to them after the conversion of marks
into grade as per the procedure mentioned below.
The assessment of Part ‘A’ i.e. Internal Assessment as mentioned above for the Se
mesters V & VI shall be processed by the colleges / Institutions of their learners’ admitted for
the programme.
For part B that is semester end examination of the semesters V & VI, the University
shall conduct the assessment.
The Internal Assessment marks of learners appearing for Semesters V & VI shall be sub
mitted to the University by the respective colleges/ Institutions before the commencement of resp
ective Semester End Examinations. The Semester End Examinations for Semesters V & VIshall
be conducted by the University and the results shall be declared after processing the internal asse
ssment and the marks awarded to the learners. The grade card shall be issued by the University af
ter converting the marks into grades.
For Part D, As per UGC guidelines fo r B.Voc. course, Skill Assessment examination
will be conducted by Healthcare Sector Skill Council (HSSC) through Assessment Agencies
of HSSC at semesters I , IV and VI. The assessment criteria will be finalised by HSSC and

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the results provided by HSSC is to be included in the student marksheet for 18 credit
points. The passing standards of skill assessment will be as per HSSC -NSDC rules.
Skill Component Examination of Semester II& III will be conducted by college and
Sem V by University as per standard rules and procedures of University of
Mumbai,mentioned in Point A, B, C.

3. Eligibility for Admission:
(Circular No. UG/33 of 2014, Dated 11th September 2014)
12th Pass from any stream from any recognised state H.S.C. board or equivalent
Candidates for being eligible for admission to the 3year integrated course leading to the
Degree of B.Voc.( M.L.T,), shall be required to have passed the Higher Secondary School Certif
icate Examination (Academic or Vocational Stream) conducted by different Divisional Boards o
f Maharashtra State Board of Secondary and Higher Secondary Education or an Examinations of
any another University or Body recognized as equivalent thereof by the Senateof the University
of Mumbai.
4. Course Duration and Level of Awards: -
(Circular No. UG/16 of 2015, Dated 13th July 2015)
The course duration and certificatio n levels will lead to Diploma / Advanced Diploma/
B.Voc. Degree in Medical Laboratory Technology. Diploma (Sem I & II) and Advanced
Diploma (Sem III & IV) to be awarded by respective colleges and the final semester V & Vl
examinations will be conducted b y University of Mumbai for award of B.Voc. Degree.
Diploma (Medical Laboratory Technology) : - 1 Year (Two Semesters ( I & II))
Adv.Diploma (Medical Laboratory Technology) : - 2 Years (Four Semesters (I , II, III, IV))
B.Voc.( Medical Laboratory Technology) : - 3 Years (Six Semesters(I, II, III,IV, V. VI))

5. Student In‐take capacity:
One division of 50 seats each in Diploma, Advanced Diploma and B.Voc. Degree.
6. Course Contents: -
According to B.Voc. guidelines of UGC, The curriculum in each of the years of the
B.Voc. programme would be a suitable mix of General Education and Skill Development
Components. Out of 30 credits per semester, the general Education component shall have 40 %
of the total credits (12 credit points per Semester) and balance 60 % c redits ( 18 credit points per
semester ) will be of skill component.

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7. PERFORMANCE GRADING:
The Performance grading of the learners shall be on the 10 Point ranking system as under.

Percentage of Marks Obtained
Grade Point Grade Performance
80.00 and above 10 O Outstanding
70-79.99 9 A+ Excellent
60-69.99 8 A Very Good
55-59.99 7 B+ Good
50-54.99 6 B Above Average
45-49.99 5 C Average
40-44.99 4 D Pass
Less than 40 0 F Fail

The Performance grading shall be based on the aggregate performance of Internal
Assessment and Semester End Examination.
8. PASSING STANDARD
The Learners to pass course shall have to obtain a minimum of 40% marks in
aggregate for each course where the course consist of Internal Assessment and Semester End
Exam ination. The Learners shall obtain minimum 40% marks (ie.10 Out of 25) in the internal
assessment and 40% marks in Semester End Examination (ie.30 out of 75) separately, to pass the course and minimum of Grade D in each project wherever applicable, to pas s the particular
semester. A Learner will be said to have passed the course if the learner passes the internal assessment & Semester End Examination together.
The Passing standard for skill component assessment by Healthcare Sector Skill
Council ( HSSC) under NSDC – UGC will be as per rule / assessment criteria of H SSC.

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Vidya Vikas Education Society’s
Vikas Night College of Arts, Science and Commerce
Kannamwar Nagar -2, Vikhroli (East), Mumbai 400083

Bachelor of Vocation (Medical Laboratory Technology)
B.Voc. (MLT) Syllabus
Year 1 (Diploma)
CBCS (75:25) Pattern
Semester I
Course Code Component Unit Topic Credits Internal
Marks External
Marks Total
Marks
BMLT101
(General &
Human
Anatomy ,
Physiology –I) Skill I Basics of Human Anatomy -I

02

25


75

100 Skill II Basics of Physiology -I
Skill III Basic English
Skill IV Human Values and Professional Ethics
BMLT102
(Routine
Laboratory
Techniques -I) Skill I Human Healthcare and Safety
Regulations


02

25


75

100 Skill II Introduction to Haematology and
Routine tests
Skill III Specimen Collection
Skill IV Laboratory Preparation in Hematology
BMLT103
(Special
Laboratory
Techniques -I) Skill I Biochemical Test Profile -I

02


25


75

100 Skill II Biochemical Test Profile – II
Skill III Elementary Knowledge of Chemistry - I
Skill IV Elementary Knowledge of Chemistry - II
BMLT104
(Cell Biology
and
Biodiversity –I) General I Microscopy and Organization of Cell -I


01

25


75

100 General II Microscopy and Organization of Cell -II
General III Systematic study of Animals - I
General IV Systematic study of Animals - II

BMLT105
(Biomolecules) General I Structure, Functions and Classification
of Amino Acids and Proteins



01



25




75



100 General II Structure, Functions and Classification
of Carbohydrates
General III Structure, Functions and Classification
of Lipids
General IV Physical and Chemical Properties of
Nucleic Acids
BMLT106
(Fundamentals
of
Microbiology) General I Introductory Microbiology -1

01

25


75

100 General II Morphology and Structure of
Microorganisms
General III Recombinant DNA Technology
General IV Microbial Ecology and Biotic
Interactions
BMLTP101 Practicals of Course BMLT101 03 50 50
BMLTP102 Practicals of Course BMLT102 50 50
BMLTP103 Practicals of Course BMLT103 50 50
BMLTP104 Practicals of Course BMLT104 50 50
BMLTP105 Practicals of Course BMLT105 50 50
BMLTP106 Practicals of Course BMLT106 50 50
BMLTP10 7 Skill Component – I
(Skill Assessment of the course will be done by sector skill council under NSDC
–
UGC. The passing standards are as
per rule and assessment criteria of
sector skill council.10 point grading 18

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scale will be applicable. Results of skill
assessment by sector sk ill council will be
included in the final marksheet of
student and will be considered for
calculation of final grade)
Total Credits 30


Bachelor of Vocation (Medical Laboratory Technology)
B.Voc. (MLT) Syllabus
Year 1 (Diploma)
CBCS (75:25) Pattern
Semester II

Course Code Component Unit Topic Credits Internal
Marks External
Marks Total
Marks
BMLT107
(General &
Human
Anatomy ,
Physiology –II) Skill I Basics of Human Anatomy -II

04

25


75

100 Skill II Basics of Physiology -II
Skill III Basics of Computer Skills
Skill IV Communication Skills

BMLT108
(Routine
Laboratory
Techniques -II) Skill I Routinue Haematological Tests

04

25


75

100 Skill II Urine Examination
Skill III Stool Examination
Skill IV Sputum and Semen Examination
BMLT109
(Special
Laboratory
Techniques -II) Skill I Basic Microbiology

04

25


75

100 Skill II Introduction to serology
Skill III Serological Tests
Skill IV Staining Techniques
BMLT110
(Ecology and
Biodiversity -II) General I Systematic study of Animals - III

02

25


75

100 General II Systematic study of Animals - IV
General III Ecosystem -I
General IV Ecosystem -II
BMLT111
(Enzymology
and
Bioenergetics) General I Enzymes

02

25


75

100 General II Enzyme Purification and
Chromatography Techniques
General III Enzyme Kinetics
General IV Bioenergetics
BMLT112
Microbial
Physiology -
Metabolism
General I Microbial Nutrition, Cultivation,
Isolation and Preservation

02

25


75

100 General II Enzyme Regulation
General III Microbial Metabolism -I
General IV Microbial Metabolism –II
BMLTP107 Practicals of Course BMLT107 02
02
02
02
02
02
50 50
BMLTP108 Practicals of Course BMLT108 50 50
BMLTP109 Practicals of Course BMLT109 50 50
BMLTP110 Practicals of Course BMLT110 50 50
BMLTP111 Practicals of Course BMLT111 50 50
BMLTP112 Practicals of Course BMLT112 50 50
Total Credits 30

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Vidya Vikas Education Society’s
Vikas Night College of Arts, Science and Commerce
Kannamwar Nagar -2, Vikhroli (East), Mumbai 400083

Bachelor of Vocation (Medical Laboratory Technology)

B.Voc. (MLT) Syllabus

Year 2 (Advanced Diploma)
CBCS (75:25) Pattern
Semester III

Course Code Component Unit Topic Credits Internal External Total
BMLT201
(Hematology
and Blood
Banking -I) Skill I Special Heamatological Tests


04

25

75

100 Skill II Haemostasis & Bleeding
Disorders
Skill III Immunohaematology & Blood
Transfusion
Skill IV Routine Lab Procedures in
Blood Bank

BMLT202
(Microbiology
and Serology) Skill I Laboratory Diagnosis of
Mycotic and Emerging
Infections


04

25

75

100
Skill II Diagnostic Microbiology
Skill III Serology
Skill IV Bacteriology

BMLT203
(Clinical
Pathology and
Biochemistry) Skill I Miscellaneous Body Fluids

04

25

75

100 Skill II Biochemical Test Profile
Skill III Analytical Techniques
Skill IV Biochemical Processes
BMLT204
(Metabolism) General I Carbohydrate Metabolism

04

25

75

100 General II Lipid Metabolism
General III Protein Metabolism
General IV Nucleic Acids

BMLT205
(Pathogenic
Microbiology) General I Infectious Diseases

04

25

75

100 General II Microbes of Medical
Importance
General III Mode of Microbial Infections
General IV Antimicrobial Drugs

BMLTP201 Practicals of Course BMLT201 02 50 50
BMLTP202 Practicals of Course BMLT202 02 50 50
BMLTP203 Practicals of Course BMLT203 02 50 50
BMLTP204 Practicals of Course BMLT204 02 50 50
BMLTP205 Practicals of Course BMLT205 02 50 50

Total Credits 30

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Bachelor of Vocation (Medical Laboratory Technology)
B.Voc. (MLT) Syllabus
Year 2 (Advanced Diploma)
CBCS (75:25) Pattern
Semester IV
Course Code Component Unit Topic Credits Internal
Marks External
Marks Total
Marks
BMLT206
(Clinical
Biochemistry
and
Microbiology-
I) Skill I Metabolic Disorders &
Deficiency

02

25

75

100 Skill II Clinical Endocrinology
Skill III Body Fluid Specimen
Processing
Skill IV Blood Banking

BMLT207
(Histolohy -
Cytology –I) Skill I Introduction to Histology

02

25

75

100 Skill II Tissue Processing
Skill III Staining Procedures
Skill IV Instrumentation in
Histocytotechnology
BMLT208
(Parasitology
and Blood
Cell
Diso rders -I) Skill I Medical Parasitology

02

25

75

100 Skill II Common Intestinal worms
Skill III Malarial parasites, Filarial
parasites
Skill IV Lab. diagnosis of Parasitic
infections
BMLT209
(Biochemical
Techniques) General I Spectroscopic Techniques

02

25

75

100 General II Electrophoretic Techniques
General III Chromatographic Techniques
General IV Radio Isotopic Techniques
BMLT210
(Immunology) General I Introduction to Immunology

02

25

75

100 General II Humoral Immunity
General III Cell Mediated Immunity
General IV Antigen -Antibody Interactions
BMLTP206 Practicals of Course BMLT206

02 50 50
BMLTP207 Practicals of Course BMLT207 50 50
BMLTP208 Practicals of Course BMLT208 50 50
BMLTP209 Practicals of Course BMLT209 50 50
BMLTP210 Practicals of Course BMLT210 50 50
BMLTP 211 Skill Component – II
(Skill Assessment of the course
will be done by sector skill
council under NSDC – UGC.
The passing standards are as
per rule and assessment criteria
of sector skill council.
10 point grading scale will be
applicable. Results of skill
assessment by sector skill
council will be included in the final m arksheet of student and
will be considered for
calculation of final grade )



18
Total Credits 30

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Vidya Vikas Education Society’s
Vikas Night College of Arts, Science and Commerce
Kannamwar Nagar -2, Vikhroli (East), Mumbai 400083

Bachelor of Vocation (Medical Laboratory Technology)

B.Voc. (MLT) Syllabus
Year 3 (B.Voc. Degree)
CBCS (75:25) Pattern
Semester V

Course Code Component Unit Topic Credits Internal
Marks External
Marks Total
Marks
BMLT301
(Medical
Genetics and
Microbiology-
II) Skill I Genetics

05

25

75

100 Skill II CLIA techniques
Skill III Immunology and Virology
Skill IV Toxicology


BMLT302
(Histology-
Cytology –II) Skill I Exfoliative Cytology -
Specimen Preparation


05

25


75

100 Skill II Exfoliative Cytology -
Staining Techniques
Skill III Exfoliative Cytology -
Benign and Malignant
Cells
Skill IV Advanced
Instrumentation

BMLT303
(Parasitology
and Blood Cell
Disorders -II) General I Descriptive study of RBC
abnormalities

04

25

75

100 General II Disorders related to RBC

General III Normal White Cell Count
& Physiological variation
General IV Disorders related to WBC
BMLT304
(Pathogenic
Microbiology) General I Pathogenic Microbes,
Diagnosis, Prevention and
Control


04

25


75

100
General II Prevention and Control of
Viral Diseases
General III Human Mycotic
Infections
General IV Mechanisms and Control
of Parasitic Infections
BMLTP301 Practicals of Course
BMLT301 04 50 50
BMLTP302 Practicals of Course
BMLT302 04 50 50
BMLTP303 Practicals of Course
BMLT303 02 50 50
BMLTP304 Practicals of Course
BMLT304 02 50 50

Total Credits 30

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Vidya Vikas Education Society’s
Vikas Night College of Arts, Science and Commerce
Kannamwar Nagar -2, Vikhroli (East), Mumbai 400083

Bachelor of Vocation (Medical Laboratory Technology)
B.Voc. (MLT) Syllabus
Year 3 (B.Voc. Degree)
CBCS (75:25) Pattern
Semester VI
Course Code Component Unit Topic
Credits Internal
Marks External
Marks Total
Marks
BMLT305
(Clinical
Laboratory
Operations
and
Management)
Skill
I

Clinical Laboratory
Operations and
Management

02

25

75

100


BMLT306
(Professional
Training)
Skill
I
Professional Training for
three (3) months at reputed
hospital, diagnostic centre,
pathology laboratory,
research institute,
pharmaceutical industry,
etc.


03

100

100

200


BMLT307
(Project
Work)
Skill
I Student shall carry out the
project work in
consultation with faculty
and industrial partner
organizations.

03

100

100

200


BMLT308
(Food and
Industrial
Microbiology) General I Food Microbiology


02



25



75



100 General II Contamination,
Preservation and Spoilage
of Food
General III Production Strains
Isolation and Screening
Techniques
General IV Fermentation Products
BMLTP305 Practicals of Course
BMLT305 01 50 50
BMLTP308 Practicals of Course
BMLT308 01 50 50
BMLTP309 Skill Component – III
(Skill Assessment of the
course will be done by
sector skill council under
NSDC – UGC. The passing
standards are as per rule and assessment criteria of sector skill council.
10 point grading scale will be applicable. Results of
skill assessment by sector
skill council will be included in the final
marksheet of student and
will be considered for
calculation of final grade)





18
Total Credits 30

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Vidya Vikas Education Soc iety’s
Vikas Night College of Arts, Science and Commerce
Kannamwar Nagar -2, Vikhroli (East), Mumbai 400083

Bachelor of Voc ation (Medical Laboratory Technology)

B.Voc. (MLT) Syllabus

Year 1 (Diploma)
CBCS (75:25) Pattern
Semester I
Part A: Skill Component

Course
Code Title Credit
s NOS

BMLT
101 General & Human Anatomy , Physiology -I 02
Basics of Human Anatomy -I


Unit I

Introduction to: Anatomy, epithelial tissue, muscular tissue,
nervous tissue. Skeletal System, Structure of bones, types of
bones, Bones of cranium, face vertebral column upper and
lower limbs. HSS/N0302/N030
4/N0305

Circulation System: Structure of heart, names and position of
main blood vessels. HSS/N0302/N030
4/N0305
Lymphatic System: Lymph vessels, lymph nodes and
lymphoid organs, their structure & functions. HSS/N0302/N030
4/N0305

Digestive systems.: Parts of gastrointestinal tract and
associated glands.(names) HSS/N0302/N030
4/N0305
Respiratory System:. Parts of Respiratory System.(diagram
,Name, function) HSS/N0302/N030
4/N0305



Unit II Basics of Physiology - I
Blood. Composition and function of blood, haemopoesis,
blood coagulation. Blood groups, body fluid. Cardiovascular Systems. Circulation of blood. function of heart and blood vessels. Control of heart rate, blood volume.(Diagram of heart and Functions in details) HSS/N0302/N030
4/N0305
Respiratory system.: Function of lungs, (theory) Respiration
disorders like anoxia.dyspnea . (Theory) lung function tests.(theory) HSS/N0302/N030
4/N0305
Digestive Systems:. Digestion of food in mouth, stomach &
small intestines. Absorption of food, function of liver. (
formation of bilirubin & other functions in detail) HSS/N0302/N030
4/N0305

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Unit
III Basic English
Grammar
Use of Articles and Prepositions, Tense, Transformation of
Sentences, Parts of Speech, Idioms and Pharses, Vocabulary a) Synonyms b) Antonyms c) One Word Substitution d) Homophones & Homonyms, Punctuations,
Common Errors, Spelling in English HSS/N 9603/N960
4/N9605/N9607
Composition
Formal & Informal Writing, Precise, Essay Writing, Report
Writing, HSS/N9603/N960
4/N9605/N9607
Reading Comprehension HSS/N9603/N960
4/N9605/N9607





Unit
IV Human Values and Professional Ethics
Introduction
Need, basic guidelines, content and process for Value
Education, Self Exploration- its content and process;
‘Natural Acceptance’ and Experiential Validation - as the
mechanism for self exploration. Continuous Happiness and
Prosperity - basic Human Aspiratio ns, Right , Relationship
and Physical Facilities - the basic requirements for fulfillment
of aspirations of every human being with their correct
priority, Happiness and Prosperity .A critical appraisal of the
current scenario, Method to fulfill the above human
aspirations: understanding and living in harmony at various
levels
Implications of Harmony on Professional Ethics
Natural acceptance of human values, Definitiveness of
Ethical Human Conduct,
Basis for Humanistic Education, Humanistic Constitution
and Humanistic Universal Order , Competence in professional ethics: Ability to utilize the professional competence for augmenting universal human order, Ability to identify the scope and characteristics of people- friendly
and ecofriendly production systems, Ability to identify and develop appropriate technologies and management patterns for above production systems, Case studies of typical holistic technologies, management models and production systems, Strategy for transition from the prese nt state to
Universal Human Order: at the level of individual: as socially and ecologically responsible engineers, technologists and managers, at the level of society: as
mutually enriching institutions and organizations

Page 18

16

Course
Code Title Credi
ts NOS

BMLT
102 Routine Laboratory Techniques -I 02





Unit I Human Healthcare and Safety Regulations
Basic causes of accidents, common types of laboratory
accidents. First aid in laboratory HSS/N0301/N030
3/N9606
Human health and Homeostasis, medical care in India,
Medical Laboratories of developing countries, Importance of
Biomedical Waste. NABL and SOP HSS/N0301/N030
3/N9606
Organization of Laboratory
Functional components of clinical laboratories,( cleanliness,
precautions to be taken WRT patients ,reports, analysis.
Communication between physician ,patients, and the medical laboratory professional, basic needs of clinical laboratory
technician, awareness of soft skills,. HSS/N0301/N030
3/N9606
Basic Laboratory Equipments
Identif ication, use, maintenance and care of common
laboratory glassware and equipments, handling of all glassware ,,use, principle and care of centrifuge, colorimter, oven, incubator, microscope, Newber's chamber,
Autoclave.etc . HSS/N0301/N030
2/N0303/N0307/N
9606
Automation
Semiautoanalysers HSS/N0303



Unit II
Introduction to Haematology and Routine tests
Components of blood and their functions, Haematopoietic
systems of the body HSS/N0302/N030
4
Haematological Diseases
Anaemia and various types of anemias, Thalssemias,
Polycythemia, Leukemia, hemolytic disease of new
born,multiple myoloma, parasitic infections of blood HSS/N0301/N030
2/N0304

Unit III Specimen Collection
Specimen collection for hematological studies HSS/N0301/N030
2/N0303

Unit IV Laboratory Preparation in Hematology
Cleaning of Laboratory glassware in hematology HSS//N0303

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17
Course
Code Title Credits NOS

BMLT103 Special Laboratory Techniques -I 02


Unit I Biochemical Test Profile - I (Quantitative
determination of blood, plasma and serum)
Acid Phosphatase (ACP), Alkaline Phosphatase
(ALP), Amino acids, Bilirubin, Cholesterol,
Creatinine, Creatine Phosphokinase (CPK),
SGOT,SGPT,Uric Acid, Urea, TSH HSS/N0301/N0302/N0304
Biochemical Test Profile (Quantitative
determination of Urine)
Amylase, Calcium, Chlorides, Creatinine,
Sodium, Potassium, Glucose, Proteins, Urea
nitrogen, uric acid HSS/N0301/N0302/N0304



Unit II Biochemical Test Profile – II (Quantitative
determination of CSF)
Chlorides, Glucose, Proteins HSS/N0301/N0302/N0304
Sterilization Techniques
Definition & Methods, principles,
bacteriological filtration, irradiation,
tyndalization HSS/N0301/N0302/N0304

Elementary Knowledge of Chemistry - I


Unit III Elementary Knowledge of Inorganic
Chemistry
Structure of atom, atomic weight, molecular and
equivalent weight. Acids, bases and salts. pH indicators (pH meter, pH paper, universal indicator). Molar solutions, normal solutions, buffer solutions, percent solutions, saturated
solutions, standard solutions HSS/N0301/N0302/N0304/
N9602
Elementary Knowledge of Organic
Chemistry
Organic compounds, aliphatic, aromatic,
alcohol, ethers, phenols, acids, etc. HSS/N0301/N0302/N0304/
N9602

Elementary Knowledge of Chemistry - II


Unit IV Elementary Knowledge of Physical
Chemistry
Osmosis, osmotic pressure, diffusion,
hypotonic, hypertonic and isotonic solutions. Definition and classification of some colloids and crystalloids. HSS/N0301/N0302/N0304/
N9602
Elementary Knowledge of Analytical
Chemistry
Principles, instrumentation, working, uses, care,
maintenance : balances, - monopan, twopan,
toppan, centrifuges, pH meter, colorimeter, HSS/N0301/N0302/N0304/
N9602

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18
spectrophotometer, florimeter, flame
photometer, ion selective electrodes,
urinometer, chromatograph, electrophoresis, densitometer.

Part B: General Education Component

SUBJECT: ZOOLOGY
Course
Code Title Credits NOS

BMLT
104 Cell Biology and Biodiversity –I 01




UNIT I Microscopy and Organization of Cell -I
Methods in Cell Biology : Principles of light and electron
microscopes, fixation & fixatives, staining techniques.
Organisation of Cell : Extra nuclear and nuclear.
Plasma : Structure, Osmosis, active and passive transport,
endocytosis and exocytosis
Endoplasmic reticulum: Structure, types and associated
enzymes.
Mitochondria
Structure, mitochondrial enzymes and the role of
mitochondria in respiration and mitochondrial DNA.
Golgi complex : Structure and functions.
.



UNIT
II Microscopy and Organization of Cell -II
Ribosomes : Types of ribosomes, their structure and
functions.
Lysosomes : Polymorphism and their function
Centrosome : Structure and functions.
Nucleus : Structure and functions of nuclear membrane,
nucleolus and chromosomes. An elementary idea of cell transformation in Canc er.
An elementary idea of cellular basis of immunity.



UNIT
III Systematic study of Animals - I
Detailed study of the following animal types :
Protozoa : Amoeba, Paramecium and Plasmodium.
Prtozoa (Porifera) : Sycon , Cnidaria (Coelenterata): Obelia

Classification upto orders with brief ecological note and
economic importance (if any) of the following:
Protozoa : Entamoeba, Trypanosoma, Giardia,
Noctiluca, Eimeria, Opalina Vorticella, Balantidium and
Nyctotherus.
Parazoa (Porifera) : Grantia, Euplectella, Hyalonema and
Spongilla.

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19
Cnidaria (Coelenterata) : Hydra, Sertularia, Plumularia,
Obelia, Tu bularia, Bougainvillea, Porpita, Velella,
Physalia, Rhizostoma Millipora, Aurelia, Alcyonium,
Tubipora, Zoanthus, Metridium, Madrepora, Favia, Fungia and Astrangia.





UNIT
IV Systematic study of Animals - II
Detailed study of the following animal types :
Platyhelminthes: Fasciola, Taenia
Aschelminthes : Ascaris, Parasitic adaptations in
Helminths.
Annelida : Pheretima
Classification upto orders with brief ecological note and
economic importance (if any) of the f ollowing:
Platyhelminthes : Dugesia, Schistosoma and Echinococcus.
Aschelminthes: : Ascaris, Oxyuris, Wuchereria.
Annelida: Nereis, Polynoe, Eunice, Arenicola,
Aphrodite, Amphitrite, Chaetopterus, Tubifex and
Pontobdella.

SUBJECT: BIOCHEMISTRY
Course
Code Title Credits NOS

BMLT
105 Biomolecules 01
Structure, Functions and Classification of Amino Acids
and Proteins



UNIT I Amino Acids & Proteins : Introduction to Bio -chemistry.
Water as a biological solvent. Dissociation of water. Buffer
solution. Henderson Hasselbalch equation.

Amino Acids : Common structural features.
Stereoisomerism and RS system of designating
optical isomers . Classification based on the nature of
“R” groups. Amino acids present in proteins and
non-protein amino acids. Specialized role of amino
acids. Physical and Chemical properties of amino acids. Titration of amino acids.

Peptide Bonds : Rigid and plana r nature of a peptide bond.
Folding of peptide chains into regular repeating structures
(helix, pleated sheets). turn in polypeptides.
Chemical synthesis of polypeptides. Biologically active peptides.

Proteins : Levels of protein structure.
Determination of primary structure of proteins.
Forces stabilising structure and shape of

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20
proteins. Native proteins and their
conformations. Behaviour of proteins in solutions.
Salting in & salting out of proteins. Denaturation of proteins.

Structural and functional diversity of proteins, fibrous
proteins (keratins, collagen & elastin), globular proteins (hemoglobin, myoglobin) and conjugated proteins.




UNIT
II Structure, Functions and Classification of
Carbohydrates
Carbohydrates :
Definition and classification of carbohydrates.

Fischer and Haworth structures of carbohydrates. Stereoisomerism, and mutarotation. Anomeric forms of monosaccharides. Derivatives of monosaccharides (glycosides, deoxysugars, amino sugars and other derivatives of biological importance). Oligosaccharides (structure of maltose, lactose, sucrose, cellobiose, trehalose, raffinose).
Characteristic reactions of monosacchari des :
Reactions with hydrazine, hydrogen cyanide,
hydroxylamine; reduction and oxidation of sugars;
periodic acid oxidation; action of alkali upon
sugars; acylation and methylation of sugars.
Homo -and hetero- polysaccharides (structures of
amylose, amylopectin, starch, inulin, pectins,
dextrins, glycogen, cellulose, chitin). (GAGs) as
components of connective tissue. Polysaccharides
of bacterial cell well.




UNIT
III
Structure, Functions and Classification of Lipids
Lipids :
Definition and classification of fatty acids
(saturated and unsaturated). Essential fatty acids.
Important reactions of functional groups present in
fatty acids. Characteristics of fatty acids and fats (saponification, iodine, acid, acetyl and peroxide
values). Refractive index, m. p., b. p. and their relation to molecular size. Properties of glycerol. Fats as source of energy. Waxes.

Structures, characteristics and functions of lipids :
Triacylglycerols, phospholipids : lecithins
(Phosphotidyl Cholines), lysolecithins, cephalins
(Phosphotidyl ethanolamines), Phosphatidyl

Page 23

21
serines, phosphatidyl inositol, sphingomyelin s,
plasmalogens), cerebrosides, gangliosides,
sulfatides.

Lipoproteins —Composition, classification and biological
functions. Liposomes.
Terpenes and Steroids —Terpenes of biological
significance e.g. carotenes, phytol. Cholesterol and
other animal stero ls. Colour reactions of sterols.
Sterols of yeast and fungi (Mycosterols).
Phytosterols. Steroidal hormones. Bile acids.

Structure and properties of Eicosanoids -
Prostaglandins, Leukotrienes, Thromboxanes,
Prostacyclins.

Structure, sources and biochemical functions of fat soluble
vitamins.




UNIT
IV Physical and Chemical Properties of Nucleic Acids
Nucleic Acid and Porphyrins :
Nucleic Acids : Structure and properties of
purine and pyrimidine bases. Nucleosides and
nucleotides. Biologically important nucleotides.
Double helical model of DNA and forces
responsible for it. Shorthand representation of
polynucleotides. Denaturation of DNA. Physical
and chemical properties of nucleic acids. Methods
for isolation, purification and characterization of
nucleic acids. Chemical and enzymatic hydrolysis
of nucleic acids. Sequencing of polynucleotides.

Porph yrins : Porphyrin nucleus and classification
of porphyrins. Heme and other metalloporphyrins occurring in nature. Detection of Porphyrins spectrophotometrically and by fluorescence. Chemical
nature and physiol ogical significance of bile pigments.

SUBJECT: MICROBIOLOGY
Course
Code Title Credits NOS

BMLT
106 Fundamentals of Microbiology 01



UNIT I Introductory Microbiology
History, development, scope and applications of
Microbiology.

Page 24

22
Methods of Microbiology isolation of pure cultures, theory
and practice of sterilization.
Microscopic examination of micro -organism, bright
field microscopy, dark field microscopy, phase contrast microscopy, electron microscopy.
Staining of microbes, theory of Gram staining.
Nature of Microbial World : Prokaryotes and eucaryotes,
growth pattern in microbes



UNIT
II Morphology and Structure of Microorganisms
Morphology & fine structure of bacteria, fungi,
actinomycete and algae.
Organization of cell wall, cell membrane, flagella and capsules in bacteria.
Morphogenesis in bacteria, formation of spores and cysts.
Animal Viruses : Morphology, cultivation and viral disease
cycle.
Bacteriophages : Morphology, multiplication, detection and enumeration.
Biotransformation of (a) D -Sorbitol to L -Sorbose. (b)
Antibiotics.
(c) Steroids.



UNIT
III Recombinant DNA Technology
Recombinant DNA technology, genetic
engineering and gene cloning in micro-
organisms.
Strategies of genetic engineering. Restriction
enzymes, vectors, plasmids. Genetic engineering for human welfare : (a) Production of
pharmaceuticals. (b) Insect pest control. (c) Use of Genetically Engineered Micro -organisms (GEMs) for
control of pollution




UNIT
IV Microbial Ecology and Biotic Interactions
Rhizosphere & Rhizoplane micro -organisms, reasons for
increased microbial activity in rhizosphere.
Biogeochemical Cycling —Carbon cycle, Nitrogen cycle,
Phosphorus & Sulphur cycle.
Symbiotic & non- symbiotic Nitrogen fixation biofertilisers
& biopesticides.
Sewage (waste- water) treatment, chemical characteristics,
microbiological characteristics, w aste water treatment
processes.

Page 25

23
Vidya Vikas Education Society’s
Vikas Night College of Arts, Science and Commerce
Kannamwar Nagar -2, Vikhroli (East), Mumbai 400083

Bachelor of Vocation (Medical Laboratory Technology)

B.Voc. (MLT) Syllabus

Year 1 (Diploma)

Practicals for Semester I

Part A: Skill Component

Sr.
No Experiment Credits NOS
BMLTP101 (General –I & Anatomy, Physiology -I)
1 Study of Epithelial, Muscle, Nerve and mammalian blood
cells through permanent or temporary cells HSS/
N0302/N0304/N0305
2 Study of the skeletal system of human beings HSS/
N0302/N0304/N0305
3 To study human respiratory system HSS/
N0302/N0304/N0305
4 To study human circulatory system HSS/
N0302/N0304/N0305
5 To study human digestive system HSS/
N0302/N0304/N0305
6 To study the compound microscope and parts. HSS/
N0302/N0304/N0305
7 To separate the plasma and serum from given blood
sample HSS/
N0301/N0302/N0304
8 To visit the following places, meet people
visiting/living/working in that environment, understand
their life style, understand value of human life in each environment and share with them the aspects of their joys and sorrows:
1. Charitable and Government Hospitals 2. Orphanages
3. Old age homes 4. Training Institute for handicapped
5. Drug De -Addiction centers 6.
Schools in rural areas
7. Industries 8. Slums 9. Jails
(The students shall prepare their project note books during each visit mentioning their experience about life of the people to whom they visited)

Page 26

24
BMLTP102 (Routine Laboratory Technology -I)
9 To indentify and to study applications of the different
laboratory instruments.
(A)Hot air oven.
(B) centrifuge.
(C)autoclave
(D)burettes & pipettes
(E)colorimeter
(F)neubauer’s Chamber HSS/
N0301/N0303/N0307
10 Determination of haemoglobin concentration by sahil’s
method HSS/
N0301/N0302/N0304
11 Determination of haemoglobin concentration by cyanmeth
method HSS/
N0301/N0302/N0304
12 Determination of total erythrocyte(RBC) count HSS/
N0301/N0302/N0304
13 Determination of leukocyte (WBC) count HSS/
N0301/N0302/N0304
14 Determination of pack cell volume (PCV) HSS/
N0301/N0302/N0304
15 Determination of erythrocyte sedimentation rate (ESR) HSS/
N0301/N0302/N0304
16 Determination and calculation of red blood indices
MCH,MCH,MCHC HSS/
N0301/N0302/N0304
17 Study of differential leukocyte count HSS/
N0301/N0302/N0304
18 Determination of absolute Eosinoehil count HSS/
N0301/N0302/N0304
19 Determination of platelet count HSS/
N0301/N0302/N0304

BMLTP 103(Special Laboratory Technology -I)
20 Principals and working of laboratory instruments HSS/
N0301/N0303/N0307
21 Importance and methods of cleaning of glass apparatus HSS/ N0303
22 Calibration of apparatus and glasswares HSS/ N0303
23 Preparation and standardization of volumetric solutions HSS/N9602
24 Basic titration such as acid vs alkali, silver nitrate vs
sodium chloride HSS/
N0301/N0302/N0304
25 Preparation of buffer solution and measurement of their pH HSS/N9602
26 Verification of Beer Lamber’s Law HSS/
N0301/N0302/N0304
27 Determination of blood sugar level of plasma (or serum)
(a) Orthotoluidine method, (b) Glucose oxidase
method HSS/
N0301/N0302/N0304
28 Determination od the serum urea nitrogen
(a) Diacetyl monoxime method HSS/
N0301/N0302/N0304
29 Determination of serum creatinine : Alkaline picrate
method HSS/
N0301/N0302/N0304
27 Determination of serum total cholesterol HSS/

Page 27

25
N0301/N0302/N0304
28 Determination of serum bilirubin
(a) Malloy and Evelyn
(b) DMSO method HSS/
N0301/N0302/N0304
29 Determination of serum glutamate pyruvate transaminase
(SGPT) and serum glutamate Oxaloacetate transaminase
(SGOT) End point reaction HSS/
N0301/N0302/N0304
30 Sterilization Techniques HSS/
N0301/N0302/N0304

Part B: General Education Component


Course
Code Title Credits NOS

BMLTP
104 Cell Biology and Biodiversity –I
1 Classification upto orders with ecological notes
and economic importance, if any, of the
following animals :

Protozoa : (a)Examination of cultures of Euglena and
Paramecium. (b)Slides : Amoeba, Euglena, Trypanosoma, Monocystis, Paramoecium (Binary fission and cjtin ,

Parazoa (Porifera) : Specimens : Sycon, Grantia,
Euplectella, Hyalonema, Spongilla, Euspongia.

Cnidaria (Coelenterata) : (a) Specimens : Porpita, Velella, Physalia, Aurelia, Rhizostoma Metrid ium,
Millipora, Alcyonium, (b) Slides : Hydra (W.M.) Hydra with buds. Obelia (colony and medusa). Sertularia, Plumularia. Platyhelminthes : (a) Specimens : Dugesia, Fasciola, Taenia,
(b) Slides : Miracidium, Sporocyst, Redia,
Cercaria of Fas io , Scolex nottio ,Tamate
Alm n s:Ac is ma e nd fe le ric ll , An ylo t .
Ali : :Ph etima e is, Hete one i , olyno ,
Eun ce,
Arthropoda : Peripatus, Prawn, Lobster, Cancer
(Crab),
Sacculina, Eupagurus (Hermit crab), Lepas, Balanus, Apis, Lepisma (Silver Fish), Schistocerca (Locust), Poecilocerus, (Ak Grasshopper), Gryllus
(Cricket), Mantis (Preying Mantis) Cicada,

Page 28

26
Forficula (Earwig) Cimex, Scarabaeus
(Dung beetle), Agrian (Dragon fly),
Odontotermes

Mollusca: Anodonta, Mytilus, Ostrea, Cardium, Pholas,
Solen
(Razorfish) Pecten, Haliotis, Patella,
Aplysia, Doris,
Ehindermta : seschiu , Ohrxa Aneon ,
Hehordata alanossus
2. CELL BIOLOGY
Paper chromatography.
Gel electrophoresis through photographs or
through research laboratories. Familarity with TEM & SEM.
Study of different ultrastructures of cell organelles
through photographs.




Course
Code Title Credits NOS

BMLTP
105 Biomolecules
1. Qualitative tests for : (a) Carbohydrates. (b) Amino
acids and proteins , (c) Cholesterol and lipids
2. Determination of saponification value of fats
3. Determination of Iodine value of fats
4. Estimation of ascorbic acid by dye method
5. Titration curve for amino acids and determination of
pKa value
6. Verification of Beer -Lambert Law for nitrophenol or
cobalt chloride
7. Estimation of Amino acids by ninhydrin method
8. Estimation of Protein by biuret method
9. Estimation of Carbohydrate by anthrone method.

Page 29

27


Course
Code Title Credits NOS

BMLTP
106 Fundamentals of Microbiology
1. Use of microscope in examination of unstained
bacteria, fungi, algae, parasites and stained cell
preparations including simple staining, Gram’s staining, acid fast staining, capsule staining, spore staining using prokaryotic and eukaryotic cells, hanging drop
preparation.
2. Preparation of culture media, spread plates, pour plates,
selective media , differential media.
3. Separation of pure cultures and study the effect of
selective nutrients on prokaryotes.
4. Isolation of Soil Bacteria, Soil Fungi, Soil Actinomycets
5. Selective media for Soil microflora and use of growth
factors, Study of Rhizosphere interactions, Quantitative
measurements of Soil nutrients and Rhizosphere microflora and preparation of starter cultures of
Rhizobia, Azotobacter.

Course
Code Title Credits NOS

BMLT P
107 Skill Component
(Skill Assessment of the course will be
done by sector skill council under NSDC –
UGC. The passing standards are as per rule
and assessment criteria of sector skill council.
10 point grading scale will be
applicable. Results of skill assessment by sector skill council will be incl
uded in the final
marksheet of student and will be considered
for calculation of final grade)
18

Page 30

28
Vidya Vi kas Education Society’s
Vikas Night College of Arts, Science and Commerce
Kannamwar Nagar -2, Vikhroli (East), Mumbai 400083

Bachelor of Vocation (Medical Laboratory Technology)

B.Voc. (MLT) Syllabus

Year 1 (Diploma)

CBCS (75:25) Pattern
Semester II
Part A: Skill Component

Course
Code Title Credits NOS

BMLT10 7 General & Human Anatomy ,
Physiology -II 04
Basics of Human Anatomy -II
Urinary System.: Parts of Urinary
System.(name, Function) HSS/N0302/N0304/N0305


Unit I
Endocrine System: Various endocrine
glands. Thyroid. Parathyroid. Adrenal
glands pituitary pancreas. Thymus and sex glands.( detail function of each gland & clinical significance) HSS/N0302/N0304/N0305
Reproductive System. Male & female
Reproductive organs.(name & function) HSS/N0302/N0304/N0305
Nervous System.: Parts of brain, spinal
cord, peripheral nerves.(function) HSS/N0302/N0304/N0305
Basics of Human Physiology -II


Unit II Excretory Systems:. Structure &
function of kidney and urinary bladder. Mechanism of urine formation. disorders of kidney.
HSS/N0302/N0304/N0305
Reproductive Systems: Physiology of
reproductive organs. HSS/N0302/N0304/N0305
Nervous System: Neurone & its
function,. HSS/N0302/N0304/N0305




Unit III Basics of Computer Skills
Data, information,properties , Types of
information. computing files,internet,server. Introduction to computer: Introduction to associated
terms like CPU,storage devices, peripherals output & input devices etc. HSS/N9603/N9604/N 9605

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29
MS WORD: Basic. Making new
document, editing.formating the text(
text: border,color, spacing,copying the text, undo,Redo,repeate) F ormatting:
Paragraph alignment,( line spacing, paragraph spacing, paragrph indents) Borders paragraph border, shading.
Spelling and grammar, HSS/N9603/N9604/N 9605
COLUMNS: typing text by defining
columns, converting text to column &
columns to text HSS/N9603/N9604/N 9605
TABLES: selecting the table, insertion
of raw .columns text ,merging the cell converting table to text and text to table insert date ,time, foot notes header footer, end notes. MS WINDOW: making new file, folders.
saving data HSS/ N9603/N9604/N 9605





Unit IV Communication Skills

The Types of Business
Communication
Introduction, Business
Communication,The Classification, Functions & Scope of Business Communication, Internal Communication, External
Communication, HSS/N9603/N9604/N9605/N9607
The Communication Process
Elements of Communication, The
Communication Cycle, The Barriers
To Communication HSS/N9603/N9604/N9605/N9607
The Principles of Communication
Introduction, The Medium of
Communication, Accuracy, Brevity,
Clarity, Courtesy, Conclusion HSS/N9603/N9604/N9605/N9607
The Modes of Communication
Oral, written, messenger service,
postal service, FAX, Electronic mail HSS/N9603/N9604/N9605/N9607
The Essentials of Written
Communication
Office stationery and forms, telephone
and FAX equipments, Computers with internet connection HSS/N9603/N9604/N9605/N9607

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30
Course
Code Title Credits NOS

BMLT
108 Routine Laboratory Technology -II 04


Unit I Routinue Haematological Tests
Determination of hemoglobin
concentration ,determination of
haematocrit , enumeration of formed elements ,calculations of red blood cell indices - MCV, MCH,and MCHC,
Automated systems in haematology ,study of blood smear , Reticulocyte count, Erytrocyte s endimantation rate (
ESR ) Eosinophil count , platelet count HSS/N0301/N0302/N0304



Unit II Urine Examination
Urine analysis, routine examination of
urine, rapid chemical tests of Urine HSS/N0301/N0302/N0304
Clinical significance, specimen
collection, laboratory investigation, Clinical significance, specimen collection, laboratory investigation HSS/N0301/N0302/N0304



Unit III Stool Examination
Gross examination, physical examination
of stool, determination of pH, chemical examination of feces, microscopic
examination of stool specimen HSS/N0301/N0302/N0304
Clinical significance, specimen
collection, laboratory investigation, Clinical significance, specimen collection, laboratory investigation HSS/N0301/N0302/N0304




Unit IV Semen Examination
Semen analysis, routine examination of
semen, quantitative determination of
semen fructose, interpretative semen analysis, examination for the presence of sperms HSS/N0301/N0302/N0304
Sputum Examination
Indication, collection, container,
transport, preservation for different types of sputum analysis.
Physical examination and its significance, chemical examination and its significance.
Microscopic examination and its
significance. HSS/N0301/N0302/N0304

Page 33

31

Course
Code Title Credi
ts NOS

BML
T 109 Special Laboratory Technology -II 04

Unit I Basic Microbiology
Classification , morphology and physiology of bacteria,
anatomy of bacterial cell, growth requirement of bacteria -
growth curve, nutrients required. Gram positive & Gram
negative Bacteria.
Normal flora of human body. HSS/N0301/N0302/N
0304


Unit II Introduction to serology
Antigens, antibodies, structure and classes of antibodies,
monoclonal antibodies and its uses. Collection and
preparation of specimen, HSS/N0301/N0302/N
0304



Unit
III Serological Tests
Serological test for syphilis (STS),Agglutination - 4 tests
,C-reactiveprotein test ( CRP) ,Rheumatoid arthritis test
(RA) ,Serodignosis of streptococcal infection .HBsAg, HIV-1( Rapid TriDot test) Widal test, Tuberculine test HSS/N0301/N0302/N
0304


Unit
IV Staining Techniques
Gram positive & Gram negative Bacteria. Difference
between cocci & bacteria, virus( definition ,properties &
example) Sputum test for AFB HSS/N0301/N0302/N
0304

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32
Part B: General Education Component

SUBJECT: ZOOLOGY
Course
Code Title Credits NOS

BMLT
110 Ecology and Biodiversity -II 02
Systematic study of Animals - III



UNIT
I
Detailed study of the following animal types
Arthropoda : Periplanata, Prawn, Social organizations in
insects (honey bee and termite), life cycle of Anopheles and Culex.
Classification up to orders with ecological notes and
economic importance (if any) Arthropoda :
Peripatus, Prawn, Lobster, Cancer,(Crab) Sacculina,
Eupagurus (Hermit crab), Lepas, Balanus, Apis,
Lepisma (Silver Fish), Schistocerca (Locust),
Poecilocerus, (AkGrasshopper), Gryllus (Cricket), Mantis (Preying Mantis) Cicada, Forficula
(Earwig) Scarabaeus (Dung beetle), Agrian
(Dragon fly),Odontotermes, (Termite queen), Cimex
(Bed bug), Cicindela (Tiger beetle), Polistes
(Wasp), Bombyx (Silk moth), Julus
(Millipede), Scolopendra (Centipede) Palamnaeus
(Scorpion) Aranea (Spider) and Limulus (King
crab).
Systematic study of Animals – IV



UNIT
II Mollusca : Pila
Echinodermata : Asterias, Echinoderm larvae.
Hemichordata : Balanoglossus, External characters and
affinities.

Classification up to orders with ecological notes
and economic importance (if any) Mollusca : Chiton, Anodonta, Mytilus, Ostrea, Car dium,
Pholas, Solen (Razor Fish), Pecten, Haliotis, Patella, Aplysia, Doris, Limax, Loligo, Sepia, Octopus, Nautilus shell and Dentalium.
Echinodermata : Echinus, Cucumaria, Ophiothrix and Antedon. Hemichordata : Balanoglossus.

Ecosystem –I



UNIT
III Ecology - Scope of ecology and subdivisions.
Ecosystem - Components, ecological
energetics, food web, introduction to major
ecosystems of the world.
Ecological factors - Temperature, light and soil as
ecological factors.

Page 35

33
Nutrients : Biogeochemical cycles & concept of limiting
factors.
Ecological : Morphological, physiological and
behavioural adaptations in animals in different habitats.
Population : Characteristics and regulation of population.



UNIT
IV Ecosystem -II
Inter and intra - Competition, predation, parasitism,
commensalisms & specific relationships &
mutualism.
Biotic community - Characteristics, ecological
succession, ecological niche.
Natural resources - Renewable and nonrenewable natural
resources and their conservations.
Environmental Degradation. - Causes, impact and control
of environmental pollution.

SUBJECT: BIOCHEMISTRY
Course
Code Title Credits NOS

BMLT
111 Enzymology and Bioenergetics 02

UNIT I Enzymes
General Characteristics :
Introduction to enzymes. General characteristics of
enzymes. Prosthetic group. Holoenzymes, apoenzyme and cofactors. Coenzymes and their biochemical functions, assay of enzyme activity, units of enzyme activity. Active sites(s) of enzymes. IUB s ystem of
nomenclature and classification of enzymes.
Enzymes as catalysts. Theories of enzymes
catalysis : Proximity and orientation effects, acid
base catalysis, covalent catalysis. Role of metals
in enzyme catalysis.



UNIT II Enzyme Purification and Chromatography
Techniques
Enzyme Purification : Need for purification.
Preliminary fractionation procedures and precipitation
techniques, Chromatography methods : Gel filtration—, adsorption–, ion exchange –and affinity chromatography.
Types of support materials. Selection of appropriate conditions and elution procedures. Criteria of enzyme
purity.



Enzyme Kinetics
Enzyme Kinetics : Factors affecting velocity of enzyme
catalysed reactions : Enzyme concentration, pH and
temperature.

Page 36

34

UNIT
III Michaelis –Menten equation.
Determination of Km and its significance.
Enzyme inhibition. Various types of enzyme inhibitions.
Determination of Ki value.
Enzyme inhibitors and their importance.
Introduction to multisubstrate enzymes.
Allosteric enzymes and enzyme regulation.
Isoenzymes and their clinical significance.
Bioenergetics :



UNIT IV Bioenergetics
Biological systems and concept of free energy,
Endergonic processes and role of ATP & other
high energy compounds. Biological oxidations.
Redox potential. Enzymes and co- enzymes
involved in oxidations and reductions. Mitochondrial electron transport chain and
oxidative phosphorylation. Mechanism of
oxidative phosphorylation.

SUBJECT: MICROBIOLOGY

Course
Code Title Credits NOS

BMLT
112 Microbial Physiology —Metabolism 02



UNIT I Microbial Nutrition, Cultivation, Isolation and
Preservation
Microbial Nutrition: Requirements for Growth.
Physical requirement (Temperature, pH, osmotic
pressure), chemical requirements (C, N, S, P, O).
Culture Media : Chemically defined media, complex media, anaerobic growth media, selective & differ ential media, and enrichment
culture. Cultivation of Aerobes and Anaerobes.
Microbial Growth : Growth in population,
bacterial growth curve, mathematical nature
and expression, measurement of growth in
bacteria, Factors affecting growth in
microorganisms, continuous cultures and
synchronous cultures.




UNIT
II Enzyme Regulation
Enzymes and their Regulation: Chemical and physical
properties of enzymes.
Nomenclature of Enzymes.
Mechanism of enzymes action.

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35
Inhibition of enzyme action.
Regulation of enzymes.




UNIT
III Microbial Metabolism –I
Microbial Metabolism :
Respiration and fermentation. Glycolysis, Pentose Phosphate pathway, The Entner Doudoroff pathway, Fermentation.
Tricarboxylic acid cycle.
Catabolism of lipid, proteins.
Glyoxylate cycle.
Beta oxidation.




UNIT
IV Microbial Metabolism –II
Microbial Utilization of Energy & Biosynthesis :
Transport of nutrient by bacteria. Biochemical mechanisms of generation of ATP.
Synthesis of Amino Acids : Glutamate, lysine, glutamine,
serine, arginine family.
Structures and biosynthesis of cell wall peptidoglycan.
Biosynthesis of Carbohydrates (gluconeogenesis) & Phospholipids.
Replication of DNA molecules, Transcription & Translation (process of protein synthesis).
Bacterial Genetics : Conjugation, Transformation,
Transduction (generalized transduction, specialized
transduction), The Regulation of Gene Expression : Lac
operon, tryptophan operon.

Page 38

36
Vidya Vikas Education Society’s
Vikas Night College of Arts, Science and Commerce
Kannamwar Nagar -2, Vikhroli (East), Mumbai 400083

Bachelor of Vocation (Medical Laboratory Technology)

B.Voc. (MLT) Syllabus

Year 1 ( Diploma)

Practicals for Semester II
Part A: Skill Component


Sr.
No Experiment Credi
ts NOS
BML TP107 (General –I & Anatomy, Physiology -I) 02
1 To study human urinary system HSS/
N0302/N0304/N0305
2 To study human reproductive system HSS/
N0302/N0304/N0305
3 To study human nervous system HSS/
N0302/N0304/N0305
4 To measure the blood pressure of human being HSS/
N0302/N0304/N0305
5 To measure the body weight and height and calculate BMI of
a human (body mass index) HSS/
N0302/N0304/N0305
6 Introduction to the word HSS/N9603/N9604/N9
605
7 Introduction to the excel HSS/N9603/N9604/N9
605
8 Introduction to the internet HSS/N9603/N9604/N9
605

BMLTP108 (Routine Laboratory Technology -II) 02
10 To prepare of the 1/10 N HCL HSS/N9602
11 To prepare the different concentration of solutions. HSS/N9602
12 To prepare different bulbs required in the laboratory HSS/N0301/N0302/N0
304/
13 To determine the nature of the given solution HSS/N0301/N0302/N0
304/ N9602
14 To find out the normality of given solution HSS/N0301/N0302/N0
304/ N9602
15 Routine examination of urine (physical examination of urine) HSS/N0301/N0302/N0
304/ N9602
16 Determination of specific gravity of urine by urinometer and
refractormeter HSS/N0301/N0302/N0
304/ N9602
17 Chemical examination of urine. HSS/

Page 39

37
N0301/N0302/N0304
18 Microscopic examination of urine HSS/
N0301/N0302/N0304
19 Physical and chemical examination of semen HSS/
N0301/N0302/N0304
20 Microscopic examination of semen HSS/
N0301/N0302/N0304
21 Physical examination of stool HSS/
N0301/N0302/N0304
22 Chemical examination of stool HSS/
N0301/N0302/N0304
23 Microscopic examination of stool HSS/
N0301/N0302/N0304
24 Determination of reducing substances in stool HSS/
N0301/N0302/N0304
25 Routine examination of sputum HSS/
N0301/N0302/N0304

BMLTP109(Special Laboratory Technology -II) 02

26 Preparation of smear HSS/
N0301/N0302/N0304
27 Monochrome staining (simple staining) HSS/
N0301/N0302/N0304
28 Gram’s staining HSS/
N0301/N0302/N0304
29 Study of motility of capsule HSS/
N0301/N0302/N0304
30 Study of bacterial capsule HSS/
N0301/N0302/N0304
31 Study of acid fast bacilli HSS/
N0301/N0302/N0304
32 Study of malerial parasite HSS/
N0301/N0302/N0304
33 Isolation of bacteria by streak plate techniques HSS/
N0301/N0302/N0304
34 To perform qualitative widal test HSS/
N0301/N0302/N0304

(On Job Training)

Page 40

38

Part B: General Education Component
SUBJECT: ZOOLOGY

Course
Code Title Credits NOS

BMLTP
110 Ecology and Biodiversity -II 02
1. Study of the following permanent stained preparations :
L.S. and T.S. Sycon, gemmules, spicules and
spongin fibres of a sponge. T.S. Hydra (Testis and
ovary region).
T.S. Fasciola (Different regions). T.S. Ascaris (Male &
female).
T.S. Pheretima (Pharyngeal and typhlosolar
regions); setae, septal nephridia, spermathecae
and ovary of Pheretima ,Trachea, mouth parts of
Periplanata Radula and osphradium of Pila. T.S.
Star fish (Arm).
2. Preparation of the following slides :
Temporary preparation of Paramecium, mouth parts of Periplaneta (cockroach), radula of Pila & appendages of Prawn.
Preparation of permanent whole mount stained in borax carmine of Hydra, Obelia. Sertularia,
Plumularia and`Bougainvillea.
3. Dissections of the following animals :
Pheretima : Digestive, reproductive and nervous systems.
Periplanata : Digestive and nervous systems; mouth parts
and trachea. Pila : Pallial complex, digestive and nervous systems
4. ECOLOGY :Study of animal ad aptations with the
help of specimens, charts and models. Study of Zoogeographical regions and their fauna. Study of biotic components of an ecosystem. Study of different types of nests in birds.
Study & preparation of zoogeographical charts.

Course
Code Title Credits NOS

BMLTP
111 Enzymology and Bioenergetics 02
1. Assay of serum alkaline phosphatase activity
2. Effect of pH on enzyme activity
3. Effect of Temperature on enzyme activity
4. Effect of substrate concentration
on enzyme activity
5. Inhibition of alkaline phosphatase
by EDTA

Page 41

39

Course
Code Title Credits NOS

BMLT P
112 Microbial Physiology —Metabolism 02
1. Measurement of Soil Enzymes.
2. Use of ultraviolet light for its germicidal effect.
3. The replica plating technique.
4. Presumptive, confirmed and completed tests for safety of
water supplies.
5. Effect of temperature, Osmotic pressure, energy source
etc. on growth of prokaryotes
6. Relation of free oxygen to microbial growth, monitoring
of dissolved oxygen in various effluents
7. Determination of COD in Industrial effluents.
8. Effects of antimetabolites on Microbial culture (Inhibition
by Sulfanilamide).
9. Determination of Water Activity of various substrates and
assay of surface active agents.
10. Turbidimetric/spectrophotometric monitoring of growth
using liquid cultures.
Efficiency of photosynthesis in photoautotrophs.

Page 42

40
Vidya Vikas Education Society’s
Vikas Night College of Arts, Science and Commerce
Kannamwar Nagar -2, Vikhroli (East), Mumbai 400083

Bachelor of Vocation (Medical Laboratory Technology)

B.Voc. (MLT) Syllabus

Year 2 ( Advanced Diploma)
CBCS (75:25) Pattern

Semester III

Part A: Skill Component


Course
Code Title Credi
ts NOS

BMLT
201 Hematology and Blood Banking -I 04



Unit I Special Heamatological Tests
Special Heamatological tests & factors in Haemoglobin
synthesis & automation
Screening of sickle cell anaemia, Estimation of foetal
haemoglobin, Haemoglobin electrophoresis, Osmotic fragility test, Heinz body preparation, Laboratory diagnosis of protozone blood parasites, Lupus erythematosus (LE) cell preparation, Preparation of bone marrow smear for
microscopic examination , Cytochemical tests. HSS/N0301/N0302/
N0304
Autoanalysis - Electrolyte acid base balance
Acid base balance HSS/N0301/N0302/
N0304
Interpretation of lab findings in Haematology
Anaemias, Leukaemias, Miscellaneous disorders. HSS/N0301/N0302/
N0304



Unit II
Haemostasis & Bleeding Disorders
Introduction to Haemostasis & coagulation
Heamostasis,Mechanism of blood coagulation,
Fibrinolysis. HSS/N0301/N0302/
N0304
Laboratory Investigation & Bleeding Disorders
Laboratory preparation for coagulation tests , Routine
coagulation tests, (prothrombin time , plasma
recalcification time ,partial thromboplastin time , activated
partial thromboplastin time, thrombin time,Laboratory
diagnosis of bleeding disorders . HSS/N0301/N0302/
N0304

Immunohaematology & Blood Transfusion

Page 43

41

Unit
III Principles of Immunohaematology & Clinical of Blood
Transfusion
Principles of immunohaematology,Human blood group
systems,(basic ABO blood group systems, Clinical
significance of blood transfusion. HSS/N0301/N0302/
N0304
Collection & Processing of blood for transfusion
Preparation for blood collection, Blood collection,
Transportation of blood after collection , storage of blood,
Preparation and use of blood components. HSS/N0301/N0302/
N0304



Unit
IV Routine Lab Procedures in Blood Bank
Routine Lab procedures in Blood Bank
Specimen collection for blood bank, General laboratory
reagents in blood bank . Preparation of laboratory regents in blood bank ,Reporting of haemagglutination reaction, ABO blood grouping Rh blood typing Antihuman globulin (AHG) or crossmatching HSS/ N0301/N0302/N
0304
Transfusion reactions & Haemolytic Disease of a new
born
Blood transfusion process, Transfusion reaction ,
Haemolytic disease of the newborn . HSS/N0301/N0302/N
0304


Course
Code Title Credi
ts NOS

BMLT
202 Microbiology and Serology 04


Unit I Laboratory Diagnosis of Mycotic and Emerging
Infections
Introduction to Microbiology
Disease oriented microbiology,culture & sensitivity
test,aerobic, anaerobic techniques HSS/N0301/N0302/N
0304
Laboratory Diagnosis of Mycotic infections
Introduction to Fungi and parasitic fungi, specimen
collection, Laboratory diagnosis of mycotic infections,
Diagnostic mycology HSS/N0301/N0302/N
0304
Emerging / New infections in human being HSS/N0301/N0302/N
0304




Unit II Diagnostic Microbiology
Diagnostic Microbiology & Micro Techniques
Role of microbiology laboratory,specimen handling ,
laboratory records, safety Regulations,Basic procedures of Diagnostic Rapid and automation methods in Diagnostic Microbiology,Culture environments of microbes , Quality control in microbiology, Quick reference of media and biochemical tests HSS/N0301/N0302/N
0304
Lab Diagnosis of parasitic infections
Collection and handling of faecal specimen, Laboratory HSS/N0301/N0302/N

Page 44

42
techniques in parasitological investigation of stool,
Processing of specimens other than stool, Lab
identification of human parasites 0304

Serology



Unit
III Serology : Introduction & Serological Lab Procedures
Principles of immunologic reactions, serodiagnosis.
Collection and prepration of specimen,Serological test for syphilis (STS),Agglutination tests ,C -reactive protein test (
CRP) ,Rheumatoid arthritis test (RA) ,Serodiagnosis of streptococcal infection ,Serodiagnostic tests for
miscellaneous disorders, Immunologic test for pregnancy
RIA, ELISA HSS/N0301/N0302/N
0304
Parasitology
Introduction, Protozoa, Helminths, Medical Entomology HSS/N0301/N0302/N
0304




Unit
IV Bacteriology
Bacteriology
Gram positive - streptococcus, staphylococcus, bacillus,
mycobacterium, corynebacterium, Gram negative - E-coli,
Klebsiella, Salmonella, shingela, Vibrio, Pseudomonas HSS/N0301/N0302/N
0304
Diagnostic & Systemic Bacteriology
Staphylococcus, Streptococcus, spirochaetes, mycoplasma,
rickettsiae etc, Systematic grouping of pathogenic bacteria , Laboratory identification of infectious agents, Diagnosis of anaerobic infections , idenifying characteristics of common pathogenic bacteria,Antimicrobial susceptibili ty
test. IMViC, Urease, catalase, geletine liquification, coagulase, oxidase, sugar fermentation, antibiotic
sensitivity test. HSS/N0301/N0302/N
0304


Course
Code Title Credi
ts NOS

BMLT
203 Clinical Pathology and Biochemistry 04


Unit I Miscellaneous Body Fluids
Lab Examination of Miscellaneous Body Fluids
Cerebrospinal fluid ,Laboratory investigation ,Serous
fluids, Synovial fluid. HSS/N0301/N0302/
N0304
Routine Biochemical Tests
Phosphatases, transaminases, lactic dehydrogenase ,
Creatine kinase , Electrolytes ,Blood gases and
bicarbonate, Determination of serum / plasma bicarbonate HSS/N0301/N0302/N
0304

Biochemical Test Profile

Page 45

43


Unit II Normal & Abnormal Biochemical processes of the body
(Basic physiology and biochemistry of the body)
Basic physiology and biochemistry of the body ,
interrelated metabolic processes of the body. HSS/N0301/N0302/N
0304
Biochemical Test Profile
Liver tests ,Renal tests, Endocrine function tests, Lipid
profile, Transaminase, LDH, CPK, CPK -MB,
SGPT/SGOT/ Amylase.GTT HSS/N0301/N0302/N
0304




Unit
III Analytical Techniques
Basic Steps of Analytic Techniques
Basic steps in analytical chemistry, titrimetry photometry,
Electrochemistry, Immuno - chemistry, Seperation and
analysis of organic compounds HSS/N0301/N0302/
N0304
Principles of Analytic Techniques
Principles of analytical chemistry, titrimetry, photometry,
Electrochemistry, Immunochemistry. HSS/N0301/N0302/
N0304



Unit
IV Biochemical Processes
Normal & Abnormal Biochemical processes of the body
(Biochemical changes in the body under pathological
conditions)
Biochemical changes in the body under pathological
conditions. HSS/N0301/N0302/
N0304
Normal & Abnormal Biochemical processes of the body
(Functions of various organs and their clinical
assessment)
Functions of various organs and their clinical assessment HSS/N0301/N0302/
N0304

Page 46

44
Part B: General Education Component
SUBJECT: BIOCHEMISTRY
Course
Code Title Credits NOS

BMLT
204 Metabolism 04



UNIT I Carbohydrate Metabolism
Digestion & Absorption of Carbohydrates :
Metabolic Pathways of Carbohydrates, Glycolysis and
alcoholic fermentation, The Pentose Phosphate Pathway, Glucuronate and glyoxylate pathway, TCA cycle, Glycogenolysis & Glycogenesis, Gluconeogenesis, Biosynthesis of starch, Biosynthesis of
Ascorbic acid.


UNIT
II Lipid Metabolism
Digestion & Transport of Lipids : -Oxidation of fatty
acids including odd chain fatty acids. α - and ω - oxidation
of fatty acids Degradation of triglycerides and phospholipids. Formation and utilization of ketone bodies. Biosynthesis of saturated and unsaturated fatty acids. Biosynt hesis of triglycerides and phospholipids,
biosynthesis of cerebrosides; sulfatides and gangliosides. Biosynthesis of Cholesterol. Biosynthesis of Prostaglandins, Thromboxanes, Leukotrienes,
Lipoxins and Prostacyclins.




UNIT
III Protein Metabolism
Digestion of Proteins : General Reactions of Amino Acids
: Deamination, transamination and decarboxylation. Urea
cycle.
Catabolism of Carbon Skeletons of Amino Acids :
Glycine and Alanine, Serine and threonine, Phenylalanine and Tyrosine, Tryptophan, Histidine, Leucine, Valine
and Isoleucine, Cysteine and Methionine, Lysine, Glutamic acid and Glutamine, Aspartic acid and Asparagine.
Biosynthesis of Nutritionally Non -Essential Amino
Acids :
Glutamate and Glutamine, Aspartate and
Asparagine, Proline, Alanine, Cysteine &
Selenocysteine, Tyrosine, Serine, Glycine.



UNIT
IV Nucleic Acids
Nucleic Acids :
Degradation of purines and pyrimidines.
Biosynthesis of purines, pyrimidines and
nucleotides. Catabolism of Heme & F ormation of
Bile pigments. Biosynthesis of porphyrins and heme. Conjugation of bilirubin and its clinical
significance.

Page 47

45
SUBJECT: MICROBIOLOGY
Course
Code Title Credits NOS

BMLT
205 Pathogenic Microbiology 04



UNIT I Infectious Diseases
Brief introduction to terminology of Infections
diseases, Frequency of disease, Recognition of
Infectious disease, Infections, Disease cycle, Virulence and mode of transmission,
Emerging and reemerging Infectious
diseases, Global travel & Health
considerations, Nosocomial Infections.



UNIT
II Microbes of Medical Importance
Nomenclature and classification of microbes of
medical importance. Origin of the Normal Flora,
Germfree and Gnotobiotic Life, Distribution and
occurrence of Normal Flora of Skin, Eye, Respiratory Tract, Mouth, Intestinal Tract &
Genitourinary Tract.


UNIT
III Mode of Microbial Infections
Microbial adherence, Passive Penetration into
body, Active Penetration into body, Events in Infection following penetration, Microbial
virulence factors.




UNIT
IV Antimicrobial Drugs
Development of chemotherapy, General
characteristics of antimicrobial drugs, Determining level of antimicrobial activity,
Mechanism of action of antimicrobial agents,
factors influencing the effectiveness of
antimicrobial drugs, Antibacterial drugs viz .
sulfonamides, Quinolones, Penicillins,
Cephalosporins, Tetracyclines, Erythromycin,
Chloramphenicol, Drug Resistance, Antifungal
and Antiviral drugs.

Page 48

46

Vidya Vikas Education Society’s
Vikas Night College of Arts, Science and Commerce
Kannamwar Nagar -2, Vikhroli (East), Mumbai 400083

Bachelor of Vocation (Medical Laboratory Technology)

B.Voc. (MLT) Syllabus
Year 2 ( Advanced Diploma)

Practicals for Semester III
Part A: Skill Component

Sr.
No Experiment Credits NOS
BMLTP201(Hematology and Blood Banking -I) 02
1 To study sickling test using 2% sodium metabisulphite HSS/
N0301/N0302/N0304
2 Determination of reticulocyte count. HSS/
N0301/N0302/N0304
3 Determination of prothrombin time HSS/
N0301/N0302/N0304
4 Determination of glucose -6-phosphate dehydrogenase(G -6-
PD) HSS/
N0301/N0302/N0304
5 Determination of serum sodium and potassium using flame
photometer/commercial kit HSS/
N0301/N0302/N0304
6 Determination of serum chloride HSS/
N0301/N0302/N0304
7 Determination of bleeding time HSS/
N0301/N0302/N0304
8 Determination of blood clotting time
1.capillary method
2.tube method HSS/
N0301/N0302/N0304
9 Qualitative test for ABO grouping with antisera by slide
method HSS/
N0301/N0302/N0304
10 Qualitative test for ABO grouping with antisera by tube
method HSS/
N0301/N0302/N0304
11 Qualitative test for Determination of D(Rho) antigen on
human red blood cells.
1.tube method
2.slide method HSS/
N0301/N0302/N0304
12 Determination of D by tube method. HSS/
N0301/N0302/N0304
13 To perform cross matching test by saline -tube method HSS/
N0301/N0302/N0304
14 To perform direct coomb’s test HSS/
N0301/N0302/N0304
15 To perform indirect coomb’s test HSS/

Page 49

47
N0301/N0302/N0304
16 Determination of the anti -D antibody titer HSS/
N0301/N0302/N0304
17 Determination of the foetal haemoglobin HSS/
N0301/N0302/N0304

BMLTP202 (Microbiology and Serology) 02
18 Study of gram’s staining HSS/
N0301/N0302/N0304
19 Study of acid fast bacilli by ZNCF(hot stain)staining HSS/
N0301/N0302/N0304
20 Demonstration of bacterial capsule by negative staining. (
india ink method) HSS/
N0301/N0302/N0304
21 Demonstration of bacterial motility by hanging drop
preparation HSS/
N0301/N0302/N0304
22 Isolation of microorganism by streak method HSS/
N0301/N0302/N0304
23 To perform biochemical test
1.IMVic test
2.Catalase test
3.Coagulase test
4.Oxidase test
5.Gelatin liquefaction test
6.Urease test HSS/
N0301/N0302/N0304
24 Identification of organism from urine sample. HSS/
N0301/N0302/N0304
25 Identification of organism from pus sample. HSS/
N0301/N0302/N0304
26 Antibiotic sensitivity test from stalk culture or biological
specimen using commercial plates and discs HSS/
N0301/N0302/N0304
27 Identification of ova/cyst from given stool sample.
1.iodine preparation
2.saline preparation HSS/
N0301/N0302/N0304
28 Identification of malarial parasite by using blood smear. HSS/
N0301/N0302/N0304
29 To perform widal test -by tube method or slide method HSS/
N0301/N0302/N0304
30 To perform VDRL test/RPR HSS/
N0301/N0302/N0304
31 To perform RA test by latex agglutination HSS/
N0301/N0302/N0304

BMLTP203 (Clinical Pathology and Biochemistry) 02
13 Estimation of blood sugar level of plasma (or serum)
(a) orthotoluidine method (b)glucose -oxidase method HSS/
N0301/N0302/N0304
14 To perform pregnancy test by dipstick method HSS/
N0301/N0302/N0304
15 Estimation of the serum urea nitrogen. HSS/

Page 50

48
(a)diacetyl monoxime method. N0301/N0302/N0304
16 Estimation of serum creatinine.
(a)alkaline -picrate method. HSS/
N0301/N0302/N0304
17 Determination of protein in blood
Albumin, globulin HSS/
N0301/N0302/N0304
18 Determination of serum bilirubin.
(a)malloy and evelyn.
(b)DMSO method. HSS/
N0301/N0302/N0304
19 Estimation of serum total cholesterol. HSS/
N0301/N0302/N0304
20 Determination of serum glutamate pyruvate
transaminase(SGPT) and serum glutamate oxaloacette
tranasaminase(SGOT) (a)end point reaction HSS/
N0301/N0302/N0304
21 Determination of serum alkaline phosphatase HSS/
N0301/N0302/N0304
22 To perform glucose tolerance test HSS/
N0301/N0302/N0304


Part B: General Education Component

Course
Code Title Credits NOS

BMLTP
204 Metabolism 02
1. Estimation of blood glucose by the methods of (i) Folin
Wu (ii) Nelson Somogyi.
2. Isolation and assay of glycogen from rat liver.
3. Estimation of Ca+ in serum
4. Estimation of total and free cholesterol in serum.
5. Estimation of total lipids in serum by Vanillin method.
6. Estimation of proteins by Lowry’s method.
7. Estimation of Lipoproteins in plasma
8. Colorimetric estimation of inorganic phosphate.

Course
Code Title Credits NOS

BMLTP
205 Pathogenic Microbiology 02
1. Stainings – Gram’s, Alberts, ZNCF.
2. Isolation and Maintenance of Pure Cultures.
3. Physiological characteristics of bacteria and its use for
their identification.
4. Assay of antimicrobials.
5. Preparation of serum/plasma.
6. Sterilization – Introduction to autoclave, hot air oven,
filter sterilization.

Page 51

49
Vidya Vikas Education Society’s
Vikas Night College of Arts, Science and Commerce
Kannamwar Nagar -2, Vikhroli (East), Mumbai 400083

Bachelor of Vocation (Medical Laboratory Technology)

B.Voc. (MLT) Syllabus

Year 2 ( Advanced Diploma)
CBCS (75:25) Pattern
Semester IV
Part A: Skill Component

Course
Code Title Credits NOS

BMLT
206 Clinical Biochemistry and Microbiology -I 02


Unit I Metabolic Disorders & Deficiency
Diagnostic Test profile
Other than biochemical tests profiles i.e. ANC,
Arthritis, Cardiac, Hypertension, Anaemia. HSS/N0301/N0302/
N0304



Unit II Clinical Endocrinology
Hormonal studies & Clinical Endocrinology
Thyroid, Pancreas, Adrenal & Sexual glands,
hormones & it's diagnostic significance. HSS/N0301/N0302/
N0304




Unit
III Body Fluid Specimen Processing
Specimen processing for biochemical analysis
Blood, Urine, Cerebrospinal fluid, Body fluids HSS/N0301/N0302/N0304
Automation in Clinical Biochemistry L aboratory
Classification of automated systems , steps of
automation in biochemical analysis, some commonly
used automated analysers of biochemical laboratories HSS/N0301/N0302/N0304


Unit
IV Blood Banking
Blood Banking
Organization, operation, administration of bank and
maintenance of records, government regulation
(FDA) HSS/N0301/N0302/N0304

Page 52

50
Course
Code Title Credits NOS

BMLT
207 Histolog y-Cytology -I 02

Unit I Introduction to Histology
Introduction to Histology & Cytotechnology
Basic terminology , Laboratory equipment for histology
and cytology , Use and care of frequently used equipment
, Preparation of reagent solutions HSS/N0301/N0302/
N0304 / N409


Unit II Tissue Processing
Lab techniques in histology: Tissue Processing
Logging of specimen, preparation of tissues , processing
of tissues , Frozen section technique , Handling and
embedding of small tissue fragments. HSS/N0301/N0302/
N0304 / N409


Unit
III Staining Procedures
Lab techniques in histology: Staining Procedures
Routine staining procedure in histotechnology , special
stains and staining techniques , stains for particular
substances HSS/N0301/N0302/
N0304 / N409


Unit
IV Instrumentation in Histocytotechnology
Instrumentation in Histocytotechnology
Autoanalyser, Tissue Processor, Microtome HSS/N0301/N0302/
N0304 / N409
`

Course
Code Title Credits NOS

BMLT
208 Parasitology and Blood Cell Disorders -I 02
Unit I Medical Parasitology HSS/N0301/N0302/N0304

Unit II Common Intestinal worms HSS/N0301/N0302/N0304

Unit
III Malarial parasites, Filarial parasites HSS/N0301/N0302/N0304

Unit
IV Lab. diagnosis of Parasitic infections HSS/N0301/N0302/N0304

Page 53

51
Part B: General Education Component
SUBJECT: BIOCHEMISTRY
Course
Code Title Credits NOS

BMLT
209 Biochemical Techniques 02


UNIT
I Spectroscopic Techniques
Spectroscopic Techniques: Beer -Lambert’s Law.
Light absorption and its transmittance.
Determination and application of extinction
coefficient. Applications of following spectroscopic techniques in elucidating structure of
Biomolecules - Visible, U.V., i nfra-red and
fluorescence spectroscopy. ORD, C.D. and N.M.R.




UNIT
II Electrophoretic Techniques
Electrophoretic Techniques :
Principles and applications of the following
electrophoresis techniques. Paper and gel
electrophoresis, high voltage electrophoresis,
SDS-PAGE : Discontinuous electrophoresis,
isotachophoresis, isoelectric focussing and
immunoelectrophoresis.

Centrifugation Techniques :
Various centrifugation techniques and their
applications in Biochemistry. Preparative and
analytical ultra- centrifugation procedures.
Application of partial specific volume, diffusion
coefficient and viscosity measurements in the study of macr omolecules of biochemical
importance.



UNIT
III Chromatographic Techniques
Chromatographic Techniques :
General principles of chromatography and the
application of following chromatographic procedures in
isolation and purification of biomolecules : Absorption, partition, paper and thin layer chromatography. Gas liquid chromatography. High performance liquid chromatography (HPLC ), Ion exhange and Exclusion
chromatography. Affinity chromatography


UNIT
IV Radio Isotopic Techniques
Radio Isotopic Techniques :
Nature of isotopes and radioisotopes. Radioactive decay. Properties of radioactive emissions. Units of radioact ivity. Techniques used to measure radioactivity;
GM counter and liquid scintillation counting and gamma

Page 54

52
counter. Labelling of Biochemical compounds and
autoradiography. Use of radioactive tracers in the study of
enzyme reaction mechanisms and metabolic pathways. Radioimmuno assay. Biological hazards of radiation and safety measures in handling radioisotopes
SUBJECT: MICROBIOLOGY
Course
Code Title Credits NOS

BMLT
210 Immunology 02


UNIT I Introduction to Immunology
Introduction and history of Immunology, Non -
specific Defense; Physical Barriers, Chemical Barriers, Phagocytosis, Inflammation, Fever, Types of Immunity, Active & Passive Immunity, Immunological memory, Primary & Secondary Lymphoid organs , Mucosa
Associated Lymphoid tissue (MALT),
Cutaneous Associated Lymphoid Tissue
(CALT), Lymphocyte Traffic, Cells of immune system, Antigens; factors affecting
Immunogenicity, epitopes, haptens.


UNIT
II Humoral Immunity
Humoral Immune Response, Antibodies /
Immunoglobulins, Structure, function and type of
antibodies, Antigentic -combining regions of
antibodies, factors influencing antibody production, Genetic model, Multigene Organisation, generation of
antibody diversity.




UNIT
III Cell Mediated Immunity
Cell Mediated Immune System, Mechanism of
CMI, Types of effector T Cells, Helper T -cells,
Suppressor, T -cells, cytotoxic T cells, Killer T
cells, Cytokines, Lymphokines, Colony
Stimulating factors, Tumour Necrosis factor,
Interferons, Accessor y cells (Macrophages), the
Complement System, Classical and Alternate pathway, HLA, Monoclonal antibody technology and its applications, Interactions between B and T
lymphocytes.



UNIT
IV Antigen -Antibody Interactions
Antigen -Antibody Interactions : Precipitation reaction, Immuno -
diffusion test, counter current Immuno electrophoresis,
complement fixation tests, Widal test, Wasserman’s test, Weil
Felix reaction, Western Blotting, Types of vaccines.

Page 55

53
Vidya Vikas Education Society’s
Vikas Night College of Arts, Science and Commerce
Kannamwar Nagar -2, Vikhroli (East), Mumbai 400083

Bachelor of Vocation (Medical Laboratory Technology)

B.Voc. (MLT) Syllabus

Year 2 ( Advanced Diploma)

Practicals for Semester IV

Part A: Skill Component


Sr.
No Experiment Credi
ts NOS
BMLT P206 (Clinical Biochemistry and Microbiology -I) 02
1 Puncture fluid HSS/
N0301/N0302/N0304
2 Routine examination of peritoneal (ascitic) fluid HSS/
N0301/N0302/N0304
3 Routine examination of pleural fluid HSS/
N0301/N0302/N0304
4 Routine examination of synovial fluid HSS/
N0301/N0302/N0304
5 Routine examination of CSF HSS/
N0301/N0302/N0304
6 Chemical examination of CSF HSS/
N0301/N0302/N0304
7 To determine uric acid in serum. HSS/
N0301/N0302/N0304
8 To determine uric acid concentration of urine. HSS/
N0301/N0302/N0304
9 To estimate serum calcium and phosphorus HSS/
N0301/N0302/N0304
10 To estimate the concentration of serum amylase HSS/
N0301/N0302/N0304
11 To estimate the concentration of CPK total and LDH HSS/
N0301/N0302/N0304
12 To determine serum acid phosphatase HSS/
N0301/N0302/N0304
13 Determination of antistrptolysin O(ASO) HSS/
N0301/N0302/N0304
14 To perform C -reactive protein test (CRP) HSS/
N0301/N0302/N0304

BMLTP207 (Histology and Cytology -I)

Page 56

54
15 To study autoanalysers HSS/N0301/N0302/N0
304/N0409
16 Introduction to chromatography HSS/N0301/N0302/N0
304/ N0409

BMLT P208 (Parasitology and Blood Cell Disorders -I)
17 Routine examination of feces. HSS/
N0301/N0302/N0304
18 Gross examination and physical examination of stool. HSS/
N0301/N0302/N0304
19 Concentration method of microscopic stool examination HSS/
N0301/N0302/N0304
20 Microscopic examination of stool specimen. HSS/
N0301/N0302/N0304
21 Detection of malarial parasite HSS/
N0301/N0302/N0304
22 Detection of trypanosomes(the casual agent of sleeping
sickness) HSS/
N0301/N0302/N0304
23 Laboratory diagnosis of kala azar HSS/
N0301/N0302/N0304
24 Laboratory diagnosis of microfilaria(wuchereeia bancrofti) HSS/
N0301/N0302/N0304
25 Quantitative determination of serum (or plasma) igG class
antibodies to toxoplasma gondii by ELISA HSS/
N0301/N0302/N0304
26 Determination of IgM class antibodies to toxoplasma gondii
by ELISA HSS/
N0301/N0302/N0304

Page 57

55

Part B: General Education Component

Course
Code Title Credits NOS

BMLTP
209 Biochemical Techniques
1. Separation and identification of amino acids by
(i) Paper chromatography (ii) Thin layer
chromatography.
2. Separation of phospholipids by thin layer
chromatography
3. Estimation of lactic acid in blood before and after
exercise.
4. Preparation of starch from potato and its hydrolysis by
salivary amylase.

Course
Code Title Credits NOS

BMLTP
210 Immunology
1. Demonstration of Immune organs in dissected animal.
2. Demonstration of Immune cells in the smears prepared
from Immune organs.
3. Complement fixation.
4. Antigen -antibody interactions
– Agglutination
– Precipitation
– Blood grouping
– Immunodiffusion

Course
Code Title Credits NOS

BMLT P
211 Skill Component
(Skill Assessment of the course will be
done by sector skill council under NSDC –
UGC. The passing standards are as per rule
and assessment criteria of sector skill council.
10 point grading scale will be
applicable. Results of s kill assessment by sector
skill council will be included in the final
marksheet of student and will be considered
for calculation of final grade)
18

Page 58

56

Vidya Vikas Education Society’s
Vikas Night College of Arts, Science and Commerce
Kannamwar Nagar -2, Vikhroli (East), Mumbai 400083

Bachelor of Vocation (Medical Laboratory Technology)

B.Voc. (MLT) Syllabus

Year 3 ( B,Voc. Degree)
CBCS (75:25) Pattern
Semester V
Part A: Skill Component

Course
Code Title Credi
ts NOS

BMLT
301 Medical Genetics and Microbiology -II 05


Unit I Genetics
Genetics: Genetics disorders, Karyotyping,
Electrophoresis and Hybridization techniques
Introduction to Medical Genetics (Structures of DNA
RNA). Genetic of common diseases. HSS/N0301/N0302/
N0304


Unit II CLIA techniques
CLIA techniques HSS/N0301/N0302/
N0304
Care and handling of laboratory animals
Introdu ction, general care and handling, ethics and
legality in use of laboratory animals HSS/N0301/N0302/
N0304




Unit
III Immunology and Virology
Immunology
Immunity/Immune system, innate immunity, adaptive
immunity, cells and oragans involved in immune system HSS/N0301/N0302/
N0304
Virology
General characteristics of Viruses, Chemotherapy of Viral
diseases, classification of viruses, Oncogenic Viruses, RNA/DNA Viruses,AIDS, Miscellaneous viruses, Structure of viruses, lysogenic cycle, lytic cycle, smallpox,
polio, HIV,Hepatitis B HSS/N0301/N0302/
N0304


Unit
IV Toxicology
Toxicological investigation & Therapeutic drug
monitoring
Analystical Techniques, drug screening, heavy metals HSS/N0301/N0302/ N
0303 /N0304/N0305/

Page 59

57
N0306/ N0307/
N9602/N9606

Course
Code Title Credits NOS

BMLT
302 Histology -Cytology -II 05
Unit I Exfoliative Cytology -Specimen Preparation
Diagnostics Exfoliative cytology: Preparation of
specimen
Prepration of specimens for cytological evaluation , HSS/N0301/N0302/
N0304 /N0409


Unit II Exfoliative Cytology - Staining Techniques
Diagnostics Exfoliative cytology: Cytological
Stains and Staining Techniques
Cytological stains and staining techniques , HSS/N0301/N0302/N0304
/N0409


Unit
III Exfoliative Cytology - Benign and Malignant Cells
Diagnostics Exfoliative cytology: Characteristics
of Benign and malignant cells
Charecteristics of benign and malignan cells HSS/N0301/N0302/N0304
/N0409

Unit
IV Advanced Instrumentation in Laboratory
Technology HSS/N0301/N0307

Part B: General Education Component

Course
Code Title Credits NOS

BMLT
303 Parasitology and Blood Cell Disorders -II 04
Unit I Descriptive study of RBC abnormalities
Descriptive study of RBC abnormalities HSS/N0301/N0302/
N0304

Unit II Disorders related to RBC
Disorders related to RBC HSS/N0301/N0302/
N0304

Unit
III Normal white cell count & physiological variation
Normal white cell count & physiological variation HSS/N0301/N0302/
N0304

Unit Disorders related to WBC

Page 60

58
IV Disorders related to WBC HSS/N0301/N0302/
N0304


SUBJECT: MICROBIOLOGY
Course
Code Title Credits NOS

BMLT
304 Pathogenic Microbiology 04


UNIT
I Pathogenic Microbes, Diagnosis, Prevention and
Control
Introduction to important diseases caused by
Streptococcus, Pneumococcus, Neisseria,
Corynebacterium, Bacillus, Ciostridium,
enterobacteriaceae (Proteus, Shigella, Salmonella), Vibrio, Yersinia, Hemophilus,
Mycobacterium, The operative pathog enic
mechanisms, laboratory diagnosis, prevention
and control of these diseases.


UNIT
II Prevention and Control of Viral Diseases
Morphology, pathogenesis, life cycle, laboratory
diagnosis, prevention and control of viral
diseases viz. Rabies, Polio, Small pox, Herpes,
Measles, Influenza and AIDS.


UNIT
IIII Human Mycotic Infections
Introduction to Human mycotic infections viz
Cryptococcosis, Dermatophytosis,
Blastomycosis, Opportunisitc Mycosis;
Candidiasis and Aspergillosis.


UNIT
IV Mechanisms and Control of Parasitic Infections
Life cycle, pathogenic, mechanisms and control
of parasitic infections viz. amoebiasis, Kala -
azar, toxoplasmosis, ascariasis, filarasis, hook
worm infections.

Page 61

59


Vidya Vikas Education Society’s
Vikas Night College of Arts, Science and Commerce
Kannamwar Nagar -2, Vikhroli (East), Mumbai 400083

Bachelor of Vocation (Medical Laboratory Technology)

B.Voc. (MLT) Syllabus

Year 3 (B.Voc.Degree)

Practicals for Semester V
Part A: Skill Component

Sr.
No Experiment Credits NOS
BMLT P301 (Clinical Biochemistry and Microbiology -II) 04
1 To detect hepatitis -B surface antigen(HBsAg) HSS/
N0301/N0302/N0304
2 To detect HIV antibodies HSS/
N0301/N0302/N0304
3 To perform haemoglobin electrophoresis HSS/
N0301/N0302/N0304
4 To perform electrophoresis HSS/
N0301/N0302/N0304
5 To determine T4 by RIA/ELISA method HSS/
N0301/N0302/N0304
6 Visit to animal house and demonstration about care of
laboratory animals HSS/
N0301/N0302/N0304

BMLTP302 (Histology and Cytology -II) 04
7 Tissue processing by using tissue processor HSS/
N0301/N0302/N0304
8 Sharpening of the microtome knife HSS/
N0301/N0302/N0304
9 Gross examination and fixation of the specimen HSS/
N0301/N0302/N0304
10 Decalcification of calcified tissue HSS/
N0301/N0302/N0304
11 Processing of the tissue by manual method HSS/
N0301/N0302/N0304
12 Section cutting of paraffin wax embedded tissue HSS/
N0301/N0302/N0304
13 To fix the section on the slide HSS/
N0301/N0302/N0304
14 Staining of the tissue section by using hematoxylin and
eosin staining method HSS/
N0301/N0302/N0304

Page 62

60
Part B: General Education Component

BMLTP 303 (Parasitology and Blood Cell Disorders -II) 02
15 Preparation of staining of blood smear HSS/
N0301/N0302/N0304
16 Study of morphology of blood cells HSS/
N0301/N0302/N0304
17 Blood cells disorder in leukemia HSS/
N0301/N0302/N0304
18 Screening for sickle cell anemia HSS/
N0301/N0302/N0304
19 Determination of osmotic fragility of red blood cells HSS/
N0301/N0302/N0304
20 Determination of fetal hemoglobin. HSS/
N0301/N0302/N0304
21 Preparation of lupus erythromatosus(LE) cell HSS/
N0301/N0302/N0304
22 Preparation of Heinz bodies HSS/
N0301/N0302/N0304
23 Microscopic examination of bone marrow smear HSS/
N0301/N0302/N0304
24 Detection of presence of iron in bone marrow smear HSS/
N0301/N0302/N0304
25 Laboratory tests for diagnosis of aplastic anemia HSS/
N0301/N0302/N0304
26 Investigations of megaloblastic anemia HSS/
N0301/N0302/N0304
27 Laboratory tests in iron deficiency anemia HSS/
N0301/N0302/N0304
28 Laboratory test for diagnosis of hemolytic disorders. HSS/
N0301/N0302/N0304

Course
Code Title Credits NOS

BMLTP
304 Pathogenic Microbiology 02
1. Identification of both gram positive and gram
negative microorganisms on the basis of : (i)
Morphology.
(ii) Bio -chemical characteristics.
(iii) Serological reactions.
2. Demonstration of pathogens (Viruses, fungi, parasites) in
permanent mounted slides.
3. Demonstration of cysts/ovas of protozoa/Helminths.
4. Demonstration of Laboratory grown fungi on sabauraud’s
agar.
5. Germ tube test for candida albicans.
6. Demonstration of fungi through normal saline/KOH
preparation.

Page 63

61

Vidya Vikas Education Society’s
Vikas Night College of Arts, Science and Commerce
Kannamwar Nagar -2, Vikhroli (East), Mumbai 400083

Bachelor of Vocation (Medical Laboratory Technology)

B.Voc. (MLT) Syllabus

Year 3 ( B.Voc. Degree)

CBCS (75:25) Pattern

Semester VI
Part A: Skill Component


Course
Code Title Credits NOS

BMLT
305 Clinical Laboratory Operations and Management 02


Unit I Reagent preparation : The metric system, preparation of
molar, normal, percent solutions Buffers, Acid, Base, pH
( Definition and examples) Lab calculations and graphs.
100
Marks
HSS/N9602
Clinical sample collection e.g. Blood, Urine, Stool
examination, Saliva sample, Sputum sample, Semen
analysis etc. HSS/N0301/N0302
Preparing and maintaining Lab records : Labeling of
sample, ;.( making, entries storage, annexes),
management of histopathology records. HSS/N0304/N0305/N9
603 / N9604/N9605
Reporting results : a. Basic format of a test report, b.
Release of examination results
c. Alteration in reports HSS/N0304/N0305
Quality Management system : Internal and External
quality control HSS/N0306/N0307/
N9606
Biomedical waste management in a clinical laboratory :
Disposal of used samples, reagents and other biomedical
waste HSS/N9609
Calibration and Validation of Clinical Laboratory
instruments HSS/N0303
Ethics in Medical laboratory Practice : Pre -Examination
procedures, Examination procedures, Reporting of results, Preserving medical records, Access to Medical laboratory Records HSS/N0304/N0305 /
N9603 / N9604 /N9605
Audit in a Medical Laboratory HSS/N0304/N0305
Documentation HSS/N0304/N0305

Professional Training
03

Page 64

62
BMLT
306
Professional Training for three (3) months at reputed
hospital, diagnostic centre, pathology laboratory,
research institute, pharmaceutical industry, etc.
(Student shall submit the valid certificate of completion of training issued by the concern organiza tion to the
college for the award of B.Voc. degree)
(Professional Training completed / obtained by the student for 3 months will be included in this semester
for 200 marks)
200
Marks

HSS/N0307/N0308
Project Work 03
BMLT
307 Student shall carry out the project work in consultation
with faculty and industrial partner organizations.
(Project work done by the student will be included in
this semester for 200 marks) 200
Marks
HSS/N0307/N0308


Part B: General Education Component

SUBJECT: MICROBIOLOGY
Course
Code Title Credits NOS

BMLT
308 Food and Industrial Microbiology 02
Food Microbiology



UNIT I Food as a substrate for microorganisms, Nutritive value of food stuffs, effect of Hydrogen ion concentration (pH), moisture requirement on food, Important food borne diseases viz. Staphyococcal intoxication, Botulism. Salmonellosis, Shigillosis,
Qualitative and Quantitative analysis of food components (proteins, fats, lipids, carbohydrates), Microbiological examination of food products including dairy products, food
poisoning caused by bacteria and fungi.



UNIT
II Contamination, Preservation and Spoilage of Food
Contamination, preservation and spoilage in
various foods viz. cereals & cereal products
(cereal grains, flour, bread, pasta, macroni),
sugars & sugars products (Maple, Syrup, Honey, Candy), Vegetables & Fruits, Meat (Fresh meat, fresh beef, hamburger, fish), Milk
and Milk products (cheese, butter).

Production Strains Isolation and Screening

Page 65

63

UNIT
III Techniques
Production strains Isolation & screening
techniques, preservation and genetic
modification of Industrial Microorganisms,
Fermentation Media, characteristics of ideal production media, common substrates used in ideal fermentations, Batch and continuous
fermentations.



UNIT
IV Fermentation Products
Yeasts (Baker’s) and its uses, fermentation of
Beer, Wine and Alcohol, Production of
organic acids viz. acetic acid, lactic acid,
propionic and butyric acid and mixed acids.
Mass transfer in aerobic fermentation.

Page 66

64
Vidya Vikas Education Society’s
Vikas Night College of Arts, Science and Commerce
Kannamwar Nagar -2, Vikhroli (East), Mumbai 400083

Bachelor of Vocation (Medical Laboratory Technology)

B.Voc. (MLT) Syllabus
Year 3 (B.Voc. Degree)
Practicals for Semester VI
PartA: No Practicals for Professional Training / Project work

Course
Code Title Credits NOS

BMLTP
305 Clinical Laboratory Operations and Management 01
1. Reagent preparation : The metric system, preparation of
molar, normal, percent solutions Buffers, Acid, Base, pH
( Definition and examples) Lab calculations and graphs.
2. Clinical sample collection e.g. Blood, Urine, Stool
examination, Saliva sample, Sputum sample, Semen
analysis etc.
3. Preparing and maintaining Lab records : Labeling of
sample, ;.( making, entries storage, annexes),
management of histopathology records.
4. Reporting results : a. Basic format of a test report, b.
Release of examination results
c. Alteration in reports
5. Biomedical waste management in a clinical laboratory :
Disposal of used samples, reagents and other biomedical
waste
6. Calibration and Validation of Clinical Laboratory
instruments
Part B: General Education Component

Course
Code Title Credits NOS

BMLT P
308 Food and Industrial Microbiology 01
1. Quantitative examination of microbial types in raw
processed preserved food stuffs.
2. Direct microscopic determination of bacteria in raw,
pasteurized milk and reductase test.
3. Various biochemical tests and their importance in Food
Microbiology.


Course Title Credits NOS

Page 67

65
Code
BMLT P
309 Skill Component
(Skill Assessment of the course will be
done by sector skill council under NSDC –
UGC. The passing standards are as per rule
and assessment criteria of sector skill council.
10 point grading scale will be
applicable. Results of skill assessment by sector
skill council will be included in the final
marksheet of student and will be considered
for calculation of final grade)
18

Examination Pattern (Semester)
D) Internal Assessment ( 25%) = 25 Marks
• One periodical test on class instructions 20 Marks
• Active participation (attentiveness/ability to answer questions) 05 Marks

E) Theory External Examination ( 75%) = 75 Marks
iii) Duration : These examinations shall be of 21/2 Hours duration for each p aper.
iv) Theory Question Paper Pattern :
• There shall be f ive questions each of 15 marks. On each unit there will be one
question and the fifth one will be based on entire syllabus.
• All que stions shall be compulsory with internal choice within the questions.
Each question will be of 20 to 23 marks with options )
• Question may be subdivided into sub questions a, b, c…. and the allocation of
marks depend on the weightage of the topic.

F) Practical External Examination = 50 Marks for each paper
G) Skill Component :-
Skill Assessment of the course will be done by Healthcare S ector S kill
Council under NSDC – UGC. The passing standards are as per rule and assessment
criteria of Healthcare S ector S kill C ouncil.
10 point grading scale will be applicable. Results of skill assessment by
Healthcare S ector S kill C ouncil will be included in the final marksheet of student
and will be considered for ca lculation of final grade .

Page 68

66
Vidya Vikas Education Society’s
Vikas Night College of Arts, Science and Commerce
Kannamwar Nagar -2, Vikhroli (East), Mumbai 400083

Bachelor of Vocation (Medical Laboratory Technology)

B.Voc. (MLT) List Reference Books

Sr.
No. Title of the Book Author
01 A guidebook to Biochemistry Michael Yudkin
02 A Manual of Laboratory & Diagnostic
Tests (6/ e) Frances Fischbach
03 Anatomy & Physiology Ross and Wilson
04 Anatomy and Physiology N Murgesh
05 Anatomy and Physiology for nurses Evelyn Pearce
06 Anatomy and Physiology for nurses Sears
07 Anatomy and Physiology for nurses Pearson
08 Anatomy and Physiology: Understanding
the Human Body Clark
09 At the Bench : A Laboratory Navigator Kathe Barker
10 At the Helm : A Laboratory Navigator Kathe Barker
11 Atlas of haematology (5/e) G.A. McDonald
12 Bacterial Metabolism Gerhard
13 Basic Medical Laboratory techniques Barbara H. Estridge et al
14 Biochemistry Voet and Voet
15 Biochemistry Stryer
16 Biochemistry U. Satyanarayan. & U.
Chakrapani
17 Biology in the Laboratory Doris Helms
18 Biometrics Identity Sameer Nanawati
19 Biopharmaceutical and Pharmacokinetics Chatwal, G.R.
20 Biostatistics : A Foundation for Analysis in
Health Sciences Wayne W. Daniel
21 Biotechnological Innovations in Health
Care Butterworth – Heinmann
22 Calculations for Molecular Biology Stephenson
23 Cell Molecular Biology Gerald Karp
24 Churchill’s Medical Dictionary Churchill Livingstone
25 Churchill’s Medical Dictionary Churchill Livingstone
26 Clinical Biochemistry Richard Luxton
27 Clinical Diagnosis & Management by
Laboratory method0 (20/e) John Bernard Henary

Page 69

67
28 Clinical Haematology Christopher A. Ludlam
29 Clinical Laboratory Management Lynne Shore
30 Clinical Pediatric Neurology Gerals Fenichel
31 Color Atlas and Synopsis of Clinical
Dermatology Thomas Fitzpatrik
32 Color atlas of basic Histopathology ---
33 Companion to Microbiology Alan Bull and Paulin Meadow
34 Current topics in AIDS (Volume I) M.S. Gotlib
35 Di Fiore’s Atlas of Histology Di Fiore
36 Drugs for the heart Lionrl H. Opie
37 Endocrinology Headley
38 Fundamental Principles of Bacteriology Salle, S.J.
39 Gel Electrophoresis of Nucleic Acids D. RickWood and B.D. Hames
40 Gene VII Benjamin Lewin
41 Gene VIII Benjamin Lewin
42 General Microbiology Stanier
43 Haematology (International edition) Emmanuel C.Besa
44 Haematology (Pathophysiological basis for
clinical practice (3/e) Stephen M. Robinson
45 Haematology for students Practitioners Ramnik Sood
46 Hand book of Medical Laboratory
Technology (2/e) V.H. Talib
47 Hospital Acquired Infections Dr. V. Muralidhar
48 Human Physiology Andrew Davis
49 Immunology Riott
50 Immunology Rao, C.V.
51 Immunology Kuby
52 Immunology Roitt, Jonathaan Brostoff
and David Male
53 Immunology and Serology Joshi
54 Instrumental Analysis Chatwal Anand
55 Laboratory Reference Jane Roskams
56 Manual of Endocrinology and Metabolism Norman Levin
57 Medical Bacteriology Peter Hawkey
58 Medical Bacteriology Peter Hawkey
59 Medical Dictionary Oxford
60 Medical Informatics Mohan Bansal
61 Medical Laboratory Management Sangeeta Sharma et al
62 Medical Laboratory Sciences, Theory &
Practical A. Kolhatkar
63 Medical Laboratory Technology – Volume
I Kanai Mukherjee

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64 Medical Laboratory Technology – Volume
II Kanai Mukherjee
65 Medical Laboratory Technology – Volume
II Kanai Mukherjee
66 Medical Laboratory Technology Methods
& Interpretation (5/e) Ramnik Sood
67 Medical Microbiology Paniker & Satish Gupte
68 Medical Microbiology Paniker & Satish Gupte
69 Medical Mycology Dr. Jagdish Chander
70 Medical Parasitology R.L. Ichhpujani and Rajesh
Bhatia
71 Medicinal Chemistry Ashutosh Kar
72 Microbiology Pelczar
73 Microbiology Prescott
74 Molecular and antibody Probes in
Diagnosis Mathew R. Walker
75 Molecular and Antibody Probes in
Diagnosis Mathew R. Walker
76 Molecular Biology in Medicine Timothy M. Cox
77 Molecular Biology in Medicine M. Cox
78 Molecular Biotechnology Glick
79 Neurodegenerative Diseases Donald B. Calne
80 Outline of Biochemistry Conn Stumpf
81 Pharmaceutical Microbiology Hugo
82 Pharmacology H.P. Rang
83 Physicians’ Desk Reference Edward Barnhart
84 Practical Clinical Biochemistry Harold Varley
85 Practical Haematology J.B. Dacie
86 Practical Haematology (8/e) Sir John
87 Practical Medical Microbiology - (Volume
I and II) Mackie & MacCartney
88 Practical Medical Microbiology - (Volume
I and II) Mackie & MacCartney
89 Principal of Biochemistry M. A. Siddiqi
90 Principles of Biochemistry David L. Nelson
91 Principles of Biochemistry Lehninger
92 Principles of Clinical Toxicology Gosses
93 Principles of Clinical Toxicology Thomas
94 Principles of Genetics Gardner
95 Principles of Internal Medicine Isselbacher
96 Proteins and Proteomics : Laboratory
Manual Richard J. Simpson

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97 Purifying Proteins for Proteomics:
Laboratory Manual Richard J. Simpson
98 Research Methodology in Medical
Sciences Chandorkar
99 Text book of Medical Biochemistry Chaterjee & Shinde
100 Text book of Microbiology Ananthanereyan
101 Text book of Parasitology N.C. Dey
102 Text book of Parasitology Chaterjee
103 Textbook of Biochemistry and Human
Biology G.P. Talwar
104 Textbook of Medical Laboratory
Technology Godkar and Godkar
105 Textbook of Medical Physiology Guyton and Hall
106 Zinsser Microbiology Joklik and Willett

107 Oxford Practical Grammar Eastwood, John
108 Essential English Grammar Murphy, Raymond
109 Practical Communication Skills Chrissie Wright
110 Business Communication Sinha, K. K.
111 Business English Taylor, Ken.
112 English Vocbulary in Use Mccarthy, Micheal.
113 Business Letters, Emails Shirley Taylor
114 The Craft of Business Letter Writing Monipally, M. Matthew.
115 Presentations Laws Anne
116 Telephoning and Teleconferencing Skills Ken Taylor
117 Business and Administrative
Communication Locker, o. Kitty
118 Presentation Skills in English Bob Dignen
119 Public Speaking Osbrn, Micheal
120 Communication in Organisation Dalmer Fisher
121 Keep Talking : Communicative Fluiency
Activities for Language Teaching Friederike Klippel
122 A Handbook for English Language
Laboratories E. Sureshkumar
123 Cambridge Idioms Dictionary Cambridge
124 Write to the Point : How to Communicate
in business with style and purpose Salvatore J. Iacone
125 Commercial Correspondence and Office
Management R. S.N. Pillai

126 Comdex Computer Course Kit Gupta, V.
128 Computer Fundamentals P.K. Sinha
129 Excel 2010 Inside Out Dodge, Mark
130 Information Technology for Management Lucas, Henry C.

131 A Foundation Course in Value Education R R Gaur, R Sangal, G P
Bagaria, 2009

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132 Fundamentals of Ethics for Scientists &
Engineers E G Seebauer & Robert L.
Berry, 2000
133 Human Values , New Age International
Publishers A.N. Tripathy, 2003
134 Environment Biology Agarwal, K. C. 2001
135 Environment Protection and Laws Jadhav, H & Bhosale, V.M.
1995
136 Waste Water Treatment Rao M. N. & Datta A.K. 1987
137 Environment Science (1st Indian Edition) Daniel D. Chiras 2010
138 Essentials of Environment Science Joseph
139 Environment Pollution Control
Engineering Rao, C.S.
140 Perspectives in Environmental Studies Kaushik, A.
141 Clinical Biochemistry G. Guru
142 Handbook of Biochemistry M.A. Siddique
143 Textbook of Medical Biochemistry S. Ramkrishnan
144 Biochemical Techniques K. Choudhary
145 Blood Banking Operations G. Gurur
146 Blood Banking Training Manual Indian Society for Blood
Banking
147 Practical Medical Microbiology Mackie and MacCartney
148 Textbook of Medical Microbiology R. Ananthnarayan and C.K. J.
Paniker
149 Invertebrate Zoology E.L. Jordan / V.S. Verma
150 Concepts of Ecology N. Arunumugam
151 Essentials of Cytology C.B. Powar
152 Cell Biology , Genetics and Evolution N. Arunumugam
153 Zoology of F.Y.B.Sc. Volume I and II S.S. Bhattacharya
154 Zoology of S.Y.B.Sc. Volume I , II and III S.S. Bhattacharya & S.G. Yeragi
155 Molecular Biology V.S. Verma & V.V. Agrawal
156 Embryology, Ecology and Physiology N. Arunumugam
157 Manual of Zoology Ekumarnath Ayyer
158 Fundamentals of Cytogenetics & Genetics Mahabali Ram
159 Human Anatomy, Physiology & Health
Education Harie R. Berasari , Gandhi &
Goel